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The map label for this gene is prf1 [H]

Identifier: 227826548

GI number: 227826548

Start: 121794

End: 122867

Strand: Direct

Name: prf1 [H]

Synonym: M1425_0138

Alternate gene names: 227826548

Gene position: 121794-122867 (Clockwise)

Preceding gene: 227826547

Following gene: 227826550

Centisome position: 4.67

GC content: 36.5

Gene sequence:

>1074_bases
TTGAAGGCACTAATTAAGGAATTGAAGAAATGGAAAGCTCCAGCTACTGTTCTTCTGTCTTTATATATACCACCCGGTAG
ACCAATTCCAGATGTGGTTAATCTACTTAGGCAAGAGTACTCCATAGCTCAGAATATAAAGCTCAAGAGGACTAGAGATG
CAGTACTTTCCGCGATAGGTGCGGCAATAGATAGGCTGAACAAGATACCGAAGATAGATGGTAATGGTTTAGTATTGTTT
TGTGGTGAAAATTTCGATACTGAGGATTTCAAATGTTTTATGTTTTCTCCTCCTGATAAAGTTCCATTATTCTTCTATAG
AACTGACAAGGAGTTTCATGTGGAATTTTTGGAGGATATGGTGGAGGATACTGTAGTATATGGTCTTATAATTGTGGAGA
GGGATGAGGCCACTATAGGTTTACTTAAGGGAACTAGAATAGAAATTTTAGAGGAAATTGAAGGTTTTGTGCCAGGGAAG
CATATGATGGGAGGACAGTCCCAGAGGAGAATTGACAGAATAATAGACGAAATGTATCATAATTTCTTAAAAGAGGTTGG
TGAGAAAGTCAATGCGTATTTTATGCCTTTTATTCAGACGGGCAAGATGAAAGGTATATTATTAGGTGGTCCAGGATATG
CTAAGGAGGATTTCTATAAGGAAGATTATGTTGACTATAGGATAAAGAATTTAATTTTACAACCCTTAATTGACGTTTCT
GATCAAGGTGAGGTTGGATTAAGGGAAATGATAATGAGGGCTGAGGATTTATTAAAGAATCAACAATACGTTGAGGTGGA
GAAATTACTAGAGGAATTAAAATATCATTTGGCTAAGGATGATGGATTAATAATATATGGAAAGGAGCAAATTAAAAAAG
CTATGGAAATGGGAGCAGTAGAGGCTATAGTAATTCATGAGGACTCTAGTGATAAAGAGTTAGAGAAATTAGCTCAAGAT
GCGGAAAATTATGGCGTTAAGGTTTTTGTGGTTGGTGATGAAGTACCAGAGGCTGAATGGGTTAAAAAAACGTTTAATGG
CGTAGTGGGTAAGTTAAGGTATAGGCTATATTAA

Upstream 100 bases:

>100_bases
TTGATATGAAGCAAGATCGGCAGATTTTTAAGGGATAACATGTTAAAGTGATAATGATTAGGGATGAGTCTTTGACGAAT
ATGCTTTAAATAAGCAAGAA

Downstream 100 bases:

>100_bases
TTAGATTATTTTTCTTCTTTTCATTTCCATTAGTATTTTATCTAATGAGATTGGTGGGTCATTTATTCCATAATTTTCAA
CAAAATTCTTTTTAAGCTCA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: Translation termination factor aRF1 [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKALIKELKKWKAPATVLLSLYIPPGRPIPDVVNLLRQEYSIAQNIKLKRTRDAVLSAIGAAIDRLNKIPKIDGNGLVLF
CGENFDTEDFKCFMFSPPDKVPLFFYRTDKEFHVEFLEDMVEDTVVYGLIIVERDEATIGLLKGTRIEILEEIEGFVPGK
HMMGGQSQRRIDRIIDEMYHNFLKEVGEKVNAYFMPFIQTGKMKGILLGGPGYAKEDFYKEDYVDYRIKNLILQPLIDVS
DQGEVGLREMIMRAEDLLKNQQYVEVEKLLEELKYHLAKDDGLIIYGKEQIKKAMEMGAVEAIVIHEDSSDKELEKLAQD
AENYGVKVFVVGDEVPEAEWVKKTFNGVVGKLRYRLY

Sequences:

>Translated_357_residues
MKALIKELKKWKAPATVLLSLYIPPGRPIPDVVNLLRQEYSIAQNIKLKRTRDAVLSAIGAAIDRLNKIPKIDGNGLVLF
CGENFDTEDFKCFMFSPPDKVPLFFYRTDKEFHVEFLEDMVEDTVVYGLIIVERDEATIGLLKGTRIEILEEIEGFVPGK
HMMGGQSQRRIDRIIDEMYHNFLKEVGEKVNAYFMPFIQTGKMKGILLGGPGYAKEDFYKEDYVDYRIKNLILQPLIDVS
DQGEVGLREMIMRAEDLLKNQQYVEVEKLLEELKYHLAKDDGLIIYGKEQIKKAMEMGAVEAIVIHEDSSDKELEKLAQD
AENYGVKVFVVGDEVPEAEWVKKTFNGVVGKLRYRLY
>Mature_357_residues
MKALIKELKKWKAPATVLLSLYIPPGRPIPDVVNLLRQEYSIAQNIKLKRTRDAVLSAIGAAIDRLNKIPKIDGNGLVLF
CGENFDTEDFKCFMFSPPDKVPLFFYRTDKEFHVEFLEDMVEDTVVYGLIIVERDEATIGLLKGTRIEILEEIEGFVPGK
HMMGGQSQRRIDRIIDEMYHNFLKEVGEKVNAYFMPFIQTGKMKGILLGGPGYAKEDFYKEDYVDYRIKNLILQPLIDVS
DQGEVGLREMIMRAEDLLKNQQYVEVEKLLEELKYHLAKDDGLIIYGKEQIKKAMEMGAVEAIVIHEDSSDKELEKLAQD
AENYGVKVFVVGDEVPEAEWVKKTFNGVVGKLRYRLY

Specific function: Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA [H]

COG id: COG1503

COG function: function code J; Peptide chain release factor 1 (eRF1)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic release factor 1 family [H]

Homologues:

Organism=Homo sapiens, GI4759034, Length=404, Percent_Identity=29.2079207920792, Blast_Score=165, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI72000368, Length=412, Percent_Identity=27.6699029126214, Blast_Score=153, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6319619, Length=407, Percent_Identity=29.4840294840295, Blast_Score=159, Evalue=5e-40,
Organism=Drosophila melanogaster, GI28574632, Length=405, Percent_Identity=28.3950617283951, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21356433, Length=405, Percent_Identity=28.3950617283951, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24667392, Length=405, Percent_Identity=28.3950617283951, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24667388, Length=405, Percent_Identity=28.3950617283951, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24667380, Length=405, Percent_Identity=28.3950617283951, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24667384, Length=405, Percent_Identity=28.3950617283951, Blast_Score=156, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005140
- InterPro:   IPR005141
- InterPro:   IPR005142
- InterPro:   IPR020918
- InterPro:   IPR004403 [H]

Pfam domain/function: PF03463 eRF1_1; PF03464 eRF1_2; PF03465 eRF1_3 [H]

EC number: NA

Molecular weight: Translated: 40957; Mature: 40957

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALIKELKKWKAPATVLLSLYIPPGRPIPDVVNLLRQEYSIAQNIKLKRTRDAVLSAIG
CHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AAIDRLNKIPKIDGNGLVLFCGENFDTEDFKCFMFSPPDKVPLFFYRTDKEFHVEFLEDM
HHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHH
VEDTVVYGLIIVERDEATIGLLKGTRIEILEEIEGFVPGKHMMGGQSQRRIDRIIDEMYH
HHHHHEEEEEEEECCCCEEEEECCCHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHH
NFLKEVGEKVNAYFMPFIQTGKMKGILLGGPGYAKEDFYKEDYVDYRIKNLILQPLIDVS
HHHHHHHHHHCEEEECCEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DQGEVGLREMIMRAEDLLKNQQYVEVEKLLEELKYHLAKDDGLIIYGKEQIKKAMEMGAV
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHCCCE
EAIVIHEDSSDKELEKLAQDAENYGVKVFVVGDEVPEAEWVKKTFNGVVGKLRYRLY
EEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKALIKELKKWKAPATVLLSLYIPPGRPIPDVVNLLRQEYSIAQNIKLKRTRDAVLSAIG
CHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AAIDRLNKIPKIDGNGLVLFCGENFDTEDFKCFMFSPPDKVPLFFYRTDKEFHVEFLEDM
HHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHH
VEDTVVYGLIIVERDEATIGLLKGTRIEILEEIEGFVPGKHMMGGQSQRRIDRIIDEMYH
HHHHHEEEEEEEECCCCEEEEECCCHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHH
NFLKEVGEKVNAYFMPFIQTGKMKGILLGGPGYAKEDFYKEDYVDYRIKNLILQPLIDVS
HHHHHHHHHHCEEEECCEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DQGEVGLREMIMRAEDLLKNQQYVEVEKLLEELKYHLAKDDGLIIYGKEQIKKAMEMGAV
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHCCCE
EAIVIHEDSSDKELEKLAQDAENYGVKVFVVGDEVPEAEWVKKTFNGVVGKLRYRLY
EEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]