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The map label for this gene is exoA [H]

Identifier: 227826512

GI number: 227826512

Start: 88382

End: 89125

Strand: Direct

Name: exoA [H]

Synonym: M1425_0101

Alternate gene names: 227826512

Gene position: 88382-89125 (Clockwise)

Preceding gene: 227826511

Following gene: 227826514

Centisome position: 3.39

GC content: 33.6

Gene sequence:

>744_bases
GTGAAGATAGTAAGTTGGAATGTTAATGGTATACGTGCAGCTTTAAAGAAGAATCTAATAGACTTCATAGAAAATAACAT
GTTTGAAGTGATAATGTTTCAAGAAACTAAAGGTGATATAGTTCCACTTGATTTTATTATGATGGGTTATGAAGTAATTT
CATTTCCAGCAAGGAGAAAAGGATATAGTGGGGTGATGACATTAACTAAGATAAAGCCCATAAATGTGATTAAAGGTCTT
CAAATTAAGGAATTCGACGATGAAGGAAGAACAGTTACCTTAGAACTGAAGGACTTCTACGTGATTAATGCATACTTTCC
TAGGGCAGGAGATAACCTAGAAAGGTTGGACTTTAAGCTAAAATTTAATAATGAAATTGAAAATTTTGTTCTTAAATTAA
GAAAAGCTAAACCGGTAATACTTTGTGGAGATTTTAACATTGCACACCAAAATATTGATGGAGCTTTTTCTGACCCAACA
ATTCCCGGACTTACACCACAAGAGAGATCGTGGTTCTCTCACTTTCTCTCTTTAGGTTTCATTGATACTTTTAGATATCT
ACACCCAAATGTTAGAAAATATAGTTGGTGGAGCTACATGGGAAAGGCTAGGGAGAAGAATTTGGGACTAAGATTAGATT
ATTGTATAGTGTCGGAAGAGTTAAAAGATAGGATAAAAATGGCTGATATTTTGATAGATATACAAGGCTCAGATCACGCA
CCTATTATCTTAGAATTAACGTAA

Upstream 100 bases:

>100_bases
TTCTTATCGAGTGATGAAGCGAGGTACATTACTGGGCAAGTCATAGTTGCAGATGGAGGGAGAATAGATAATTTAACTCA
TTCGTTATAATCTTCTAAAT

Downstream 100 bases:

>100_bases
ATTATATTCTATAGTAGTATAGATTGTTCTAGCGTTAGTTCTTTGTAATAGCATGTCATACAAGAGGTTAACTAACCATG
ATGGCATATTATTTATTTTA

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MKIVSWNVNGIRAALKKNLIDFIENNMFEVIMFQETKGDIVPLDFIMMGYEVISFPARRKGYSGVMTLTKIKPINVIKGL
QIKEFDDEGRTVTLELKDFYVINAYFPRAGDNLERLDFKLKFNNEIENFVLKLRKAKPVILCGDFNIAHQNIDGAFSDPT
IPGLTPQERSWFSHFLSLGFIDTFRYLHPNVRKYSWWSYMGKAREKNLGLRLDYCIVSEELKDRIKMADILIDIQGSDHA
PIILELT

Sequences:

>Translated_247_residues
MKIVSWNVNGIRAALKKNLIDFIENNMFEVIMFQETKGDIVPLDFIMMGYEVISFPARRKGYSGVMTLTKIKPINVIKGL
QIKEFDDEGRTVTLELKDFYVINAYFPRAGDNLERLDFKLKFNNEIENFVLKLRKAKPVILCGDFNIAHQNIDGAFSDPT
IPGLTPQERSWFSHFLSLGFIDTFRYLHPNVRKYSWWSYMGKAREKNLGLRLDYCIVSEELKDRIKMADILIDIQGSDHA
PIILELT
>Mature_247_residues
MKIVSWNVNGIRAALKKNLIDFIENNMFEVIMFQETKGDIVPLDFIMMGYEVISFPARRKGYSGVMTLTKIKPINVIKGL
QIKEFDDEGRTVTLELKDFYVINAYFPRAGDNLERLDFKLKFNNEIENFVLKLRKAKPVILCGDFNIAHQNIDGAFSDPT
IPGLTPQERSWFSHFLSLGFIDTFRYLHPNVRKYSWWSYMGKAREKNLGLRLDYCIVSEELKDRIKMADILIDIQGSDHA
PIILELT

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=256, Percent_Identity=36.71875, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI18375503, Length=256, Percent_Identity=36.71875, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI18375501, Length=256, Percent_Identity=36.71875, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI18375507, Length=313, Percent_Identity=28.1150159744409, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1788046, Length=265, Percent_Identity=32.0754716981132, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71989536, Length=254, Percent_Identity=37.007874015748, Blast_Score=181, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI71989539, Length=143, Percent_Identity=30.7692307692308, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221330655, Length=252, Percent_Identity=39.2857142857143, Blast_Score=180, Evalue=8e-46,
Organism=Drosophila melanogaster, GI17136678, Length=252, Percent_Identity=39.2857142857143, Blast_Score=179, Evalue=1e-45,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 28638; Mature: 28638

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVSWNVNGIRAALKKNLIDFIENNMFEVIMFQETKGDIVPLDFIMMGYEVISFPARRK
CEEEEECCHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCEEEHHHHHHHHHHCCCCHHCC
GYSGVMTLTKIKPINVIKGLQIKEFDDEGRTVTLELKDFYVINAYFPRAGDNLERLDFKL
CCCCEEEEEEECCCHHHCCCEEEEECCCCCEEEEEEEEEEEEEEECCCCCCCHHHEEEEE
KFNNEIENFVLKLRKAKPVILCGDFNIAHQNIDGAFSDPTIPGLTPQERSWFSHFLSLGF
EECCHHHHHHHHHHCCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
IDTFRYLHPNVRKYSWWSYMGKAREKNLGLRLDYCIVSEELKDRIKMADILIDIQGSDHA
HHHHHHHCCCCCEEHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHEEEEEEEEECCCCCC
PIILELT
CEEEEEC
>Mature Secondary Structure
MKIVSWNVNGIRAALKKNLIDFIENNMFEVIMFQETKGDIVPLDFIMMGYEVISFPARRK
CEEEEECCHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCEEEHHHHHHHHHHCCCCHHCC
GYSGVMTLTKIKPINVIKGLQIKEFDDEGRTVTLELKDFYVINAYFPRAGDNLERLDFKL
CCCCEEEEEEECCCHHHCCCEEEEECCCCCEEEEEEEEEEEEEEECCCCCCCHHHEEEEE
KFNNEIENFVLKLRKAKPVILCGDFNIAHQNIDGAFSDPTIPGLTPQERSWFSHFLSLGF
EECCHHHHHHHHHHCCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
IDTFRYLHPNVRKYSWWSYMGKAREKNLGLRLDYCIVSEELKDRIKMADILIDIQGSDHA
HHHHHHHCCCCCEEHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHEEEEEEEEECCCCCC
PIILELT
CEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]