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The map label for this gene is fabG2 [H]

Identifier: 227826497

GI number: 227826497

Start: 73608

End: 74393

Strand: Direct

Name: fabG2 [H]

Synonym: M1425_0086

Alternate gene names: 227826497

Gene position: 73608-74393 (Clockwise)

Preceding gene: 227826496

Following gene: 227826498

Centisome position: 2.82

GC content: 37.91

Gene sequence:

>786_bases
ATGGATATGGGAATAAAGGGGAAAAAGGTAATAGTGACAGCAGCAAGTAAAGGTATAGGTTTAGCAATAGCCAAACGTTT
TTTAGAAGAGGGCGCGAAAGTAATTATTTCATCTCATGAAGAATCAAATCTAATAAAAGCCTACAATAAACTAAGGGAGA
TTTATGGTGACAATGTAAGTTATATAAAGGCAGATTTGACGAATCCACAAGAAGTTAAGTATCTAGTTCATGAAGGATAT
AGAAAACTTGGAGGCTTAGAGGTTTTAGCTTACGTTACCGGTAGTCCAAAACCTGGAAATTTATTTTCCTTAACAGATAG
GGATTGGATAGACGCTTTTAACCTACTTTTACTCAGTGCAGTTATTGCAGTCAGAGAAGCTGGTCAGCTAATGCAAAGTG
GAGGTAGGATAATTCTATCTACATCAACCACATTAAGGCAACCTATAGATAACCTCGATTTATCAAATGTTGTGAGACTC
TCATTAGCAGGCTTAATTAAGGTTGCGTCAAGGGAACTGGGACCTAAAGGAATTTTAGTAAATGGAATAATGCCTGGTTG
GACAAAGACGGAAAGGCTGCTTCAGATTGTAAGAGATAGAGCGATGAGGGATAGTAAAAGTGAAGAGGAAGTTCTTAAGG
CGATTGTCGCTGATGTACCTCTTGGTAGAGCAGGAGAACCAGAAGAGATTGCTAACGTTGTAATATTTTTGGCATCCAAT
CTTGCTACTTATGTTAACGGTACCTTAATACCAGTAGATGGAGGTAGCATAAAAGGAGTCTTTTAA

Upstream 100 bases:

>100_bases
GCTCTTTATAATCCACCTATTAGAAAAATACTAGAAGAAGATTTCAGAAAAGTTAAAGAAGCATTAAGTTCAAAACCTAT
TACTTTAGATAATTTCTTGT

Downstream 100 bases:

>100_bases
GCGTTTTTGCAGATAATACTCCGTTTTTTCTCTAACAATAGATTAACGTTAAAGAAGCCTGCTAACGATTATATTTTATT
CTGCTAAGTATTACCGTAGA

Product: short-chain dehydrogenase/reductase SDR

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MDMGIKGKKVIVTAASKGIGLAIAKRFLEEGAKVIISSHEESNLIKAYNKLREIYGDNVSYIKADLTNPQEVKYLVHEGY
RKLGGLEVLAYVTGSPKPGNLFSLTDRDWIDAFNLLLLSAVIAVREAGQLMQSGGRIILSTSTTLRQPIDNLDLSNVVRL
SLAGLIKVASRELGPKGILVNGIMPGWTKTERLLQIVRDRAMRDSKSEEEVLKAIVADVPLGRAGEPEEIANVVIFLASN
LATYVNGTLIPVDGGSIKGVF

Sequences:

>Translated_261_residues
MDMGIKGKKVIVTAASKGIGLAIAKRFLEEGAKVIISSHEESNLIKAYNKLREIYGDNVSYIKADLTNPQEVKYLVHEGY
RKLGGLEVLAYVTGSPKPGNLFSLTDRDWIDAFNLLLLSAVIAVREAGQLMQSGGRIILSTSTTLRQPIDNLDLSNVVRL
SLAGLIKVASRELGPKGILVNGIMPGWTKTERLLQIVRDRAMRDSKSEEEVLKAIVADVPLGRAGEPEEIANVVIFLASN
LATYVNGTLIPVDGGSIKGVF
>Mature_261_residues
MDMGIKGKKVIVTAASKGIGLAIAKRFLEEGAKVIISSHEESNLIKAYNKLREIYGDNVSYIKADLTNPQEVKYLVHEGY
RKLGGLEVLAYVTGSPKPGNLFSLTDRDWIDAFNLLLLSAVIAVREAGQLMQSGGRIILSTSTTLRQPIDNLDLSNVVRL
SLAGLIKVASRELGPKGILVNGIMPGWTKTERLLQIVRDRAMRDSKSEEEVLKAIVADVPLGRAGEPEEIANVVIFLASN
LATYVNGTLIPVDGGSIKGVF

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI5031737, Length=250, Percent_Identity=30.4, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI32483357, Length=256, Percent_Identity=29.296875, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI33667109, Length=188, Percent_Identity=31.9148936170213, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI126723750, Length=262, Percent_Identity=26.3358778625954, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI15277342, Length=255, Percent_Identity=25.4901960784314, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI66933014, Length=254, Percent_Identity=28.740157480315, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI10190704, Length=258, Percent_Identity=24.4186046511628, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI40254992, Length=250, Percent_Identity=25.6, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI19923817, Length=261, Percent_Identity=25.2873563218391, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI7705925, Length=261, Percent_Identity=25.6704980842912, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787905, Length=262, Percent_Identity=27.4809160305344, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787335, Length=258, Percent_Identity=30.2325581395349, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789378, Length=261, Percent_Identity=28.735632183908, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI87082160, Length=258, Percent_Identity=27.5193798449612, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1786812, Length=261, Percent_Identity=29.1187739463602, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082100, Length=258, Percent_Identity=26.7441860465116, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI2367365, Length=251, Percent_Identity=28.2868525896414, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788459, Length=251, Percent_Identity=28.6852589641434, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789208, Length=260, Percent_Identity=25.7692307692308, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790717, Length=263, Percent_Identity=24.7148288973384, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17561402, Length=254, Percent_Identity=30.7086614173228, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17555706, Length=259, Percent_Identity=32.046332046332, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17560676, Length=248, Percent_Identity=28.2258064516129, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25147288, Length=263, Percent_Identity=27.3764258555133, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17562904, Length=265, Percent_Identity=29.0566037735849, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17562910, Length=262, Percent_Identity=28.2442748091603, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17561272, Length=241, Percent_Identity=27.3858921161826, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI115534694, Length=253, Percent_Identity=26.4822134387352, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560220, Length=270, Percent_Identity=26.2962962962963, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17544670, Length=268, Percent_Identity=25, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17536651, Length=262, Percent_Identity=25.9541984732824, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI72000259, Length=260, Percent_Identity=26.5384615384615, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17536025, Length=261, Percent_Identity=24.904214559387, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI193204405, Length=268, Percent_Identity=27.2388059701493, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=259, Percent_Identity=27.4131274131274, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=258, Percent_Identity=25.968992248062, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21355319, Length=254, Percent_Identity=33.0708661417323, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24639444, Length=264, Percent_Identity=30.3030303030303, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI23397609, Length=255, Percent_Identity=30.5882352941176, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24644339, Length=255, Percent_Identity=25.8823529411765, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28571526, Length=256, Percent_Identity=26.171875, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24644337, Length=253, Percent_Identity=28.8537549407115, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21357041, Length=253, Percent_Identity=26.4822134387352, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 28255; Mature: 28255

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMGIKGKKVIVTAASKGIGLAIAKRFLEEGAKVIISSHEESNLIKAYNKLREIYGDNVS
CCCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHHHCCCCE
YIKADLTNPQEVKYLVHEGYRKLGGLEVLAYVTGSPKPGNLFSLTDRDWIDAFNLLLLSA
EEEECCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHH
VIAVREAGQLMQSGGRIILSTSTTLRQPIDNLDLSNVVRLSLAGLIKVASRELGPKGILV
HHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
NGIMPGWTKTERLLQIVRDRAMRDSKSEEEVLKAIVADVPLGRAGEPEEIANVVIFLASN
ECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LATYVNGTLIPVDGGSIKGVF
HHHHHCCEEEEECCCCCCCCC
>Mature Secondary Structure
MDMGIKGKKVIVTAASKGIGLAIAKRFLEEGAKVIISSHEESNLIKAYNKLREIYGDNVS
CCCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHHHCCCCE
YIKADLTNPQEVKYLVHEGYRKLGGLEVLAYVTGSPKPGNLFSLTDRDWIDAFNLLLLSA
EEEECCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHH
VIAVREAGQLMQSGGRIILSTSTTLRQPIDNLDLSNVVRLSLAGLIKVASRELGPKGILV
HHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
NGIMPGWTKTERLLQIVRDRAMRDSKSEEEVLKAIVADVPLGRAGEPEEIANVVIFLASN
ECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LATYVNGTLIPVDGGSIKGVF
HHHHHCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]