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The map label for this gene is mhpC [H]

Identifier: 227826484

GI number: 227826484

Start: 65477

End: 66070

Strand: Direct

Name: mhpC [H]

Synonym: M1425_0073

Alternate gene names: 227826484

Gene position: 65477-66070 (Clockwise)

Preceding gene: 227826481

Following gene: 227826485

Centisome position: 2.51

GC content: 35.69

Gene sequence:

>594_bases
ATGCAGCAGTTAATAGATAAGTTCTTAGAGGTAAAAGGACGTAAAATACATTATATTGAGAGTGGAGACGGCTCTCCAGT
TCTTTTATTTCACGGTGCGAGGTTCAATGCAAGAACGTGGGTAGAGACTAATACAGTTGACTCAATTTCGAATATAGGGT
ACAAGGCAATTTCGGTGGACTTTCCAGGATTTGGATCTTCCGAAAAGATTGAAGGCATTAGTCTCTCAGAGTTCATAAAT
CTCTTCATGAATTCTCTAGGTATCAGTAAGGCAATATTGCTGGGTGCCTCGATGGGTGGAAAGGCAGTACTAGAATTTAG
TCTTAAATATACTGAATCTGTTTATGGGCTAGTACTAGTAGGTGCTGTTGGTGTAGAGGAGTTTGAGGACCAAATATCAA
AACTAAATAAGATTCCCTTATTATTAATTTGGGGTTCTCGTGATACTGTTTCTCCTAAAAGAAATTATGAGTTAATATTA
GATAAAGTGAAAAATGCGAAACTAGAAATTGTAGGTAAAAATCACGCTTGTTATTTAGATGATCCAAATACGTTTAATGA
AAAAATTGTTAATTTTCTCAAGGGGATGAAATGA

Upstream 100 bases:

>100_bases
TATAAACTTTATTATCTCCCTTTTTCTAAAGTTATCATTAAGTATTTCATTCTGTTAAAAATGTAAAAAAGCTTATAACT
TTTTGAACCAGTAATTATTC

Downstream 100 bases:

>100_bases
GTGATAAAGAGGAATTAAGCGCTAAACTTAGGATAATATTGAGAGATATAAGATATCTTACTATTCTAAAACACTTGAAA
ATTGCTAATGTAGACTATGG

Product: hypothetical protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase [H]

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MQQLIDKFLEVKGRKIHYIESGDGSPVLLFHGARFNARTWVETNTVDSISNIGYKAISVDFPGFGSSEKIEGISLSEFIN
LFMNSLGISKAILLGASMGGKAVLEFSLKYTESVYGLVLVGAVGVEEFEDQISKLNKIPLLLIWGSRDTVSPKRNYELIL
DKVKNAKLEIVGKNHACYLDDPNTFNEKIVNFLKGMK

Sequences:

>Translated_197_residues
MQQLIDKFLEVKGRKIHYIESGDGSPVLLFHGARFNARTWVETNTVDSISNIGYKAISVDFPGFGSSEKIEGISLSEFIN
LFMNSLGISKAILLGASMGGKAVLEFSLKYTESVYGLVLVGAVGVEEFEDQISKLNKIPLLLIWGSRDTVSPKRNYELIL
DKVKNAKLEIVGKNHACYLDDPNTFNEKIVNFLKGMK
>Mature_197_residues
MQQLIDKFLEVKGRKIHYIESGDGSPVLLFHGARFNARTWVETNTVDSISNIGYKAISVDFPGFGSSEKIEGISLSEFIN
LFMNSLGISKAILLGASMGGKAVLEFSLKYTESVYGLVLVGAVGVEEFEDQISKLNKIPLLLIWGSRDTVSPKRNYELIL
DKVKNAKLEIVGKNHACYLDDPNTFNEKIVNFLKGMK

Specific function: Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mhpC alpha/beta hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI226443152, Length=201, Percent_Identity=25.8706467661692, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI14249382, Length=201, Percent_Identity=25.8706467661692, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI226437571, Length=175, Percent_Identity=24.5714285714286, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI87081721, Length=127, Percent_Identity=33.8582677165354, Blast_Score=64, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17543658, Length=188, Percent_Identity=31.3829787234043, Blast_Score=94, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 21853; Mature: 21853

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQLIDKFLEVKGRKIHYIESGDGSPVLLFHGARFNARTWVETNTVDSISNIGYKAISVD
CHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCEEEEECCCHHHHHHCCEEEEEEE
FPGFGSSEKIEGISLSEFINLFMNSLGISKAILLGASMGGKAVLEFSLKYTESVYGLVLV
CCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHEEEECCCCCCEEEEEEEHHHHHHHHEEEE
GAVGVEEFEDQISKLNKIPLLLIWGSRDTVSPKRNYELILDKVKNAKLEIVGKNHACYLD
ECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEECCCEEEEC
DPNTFNEKIVNFLKGMK
CCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MQQLIDKFLEVKGRKIHYIESGDGSPVLLFHGARFNARTWVETNTVDSISNIGYKAISVD
CHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCEEEEECCCHHHHHHCCEEEEEEE
FPGFGSSEKIEGISLSEFINLFMNSLGISKAILLGASMGGKAVLEFSLKYTESVYGLVLV
CCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHEEEECCCCCCEEEEEEEHHHHHHHHEEEE
GAVGVEEFEDQISKLNKIPLLLIWGSRDTVSPKRNYELILDKVKNAKLEIVGKNHACYLD
ECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEECCCEEEEC
DPNTFNEKIVNFLKGMK
CCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA