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The map label for this gene is pckA [H]

Identifier: 227817563

GI number: 227817563

Start: 4556502

End: 4557857

Strand: Direct

Name: pckA [H]

Synonym: BAMEG_5056

Alternate gene names: 227817563

Gene position: 4556502-4557857 (Clockwise)

Preceding gene: 227817562

Following gene: 227817565

Centisome position: 87.12

GC content: 38.79

Gene sequence:

>1356_bases
GTGAATCAACCGATTTCTGAAGAACATTTTAATAAATTATATACTAAAGTTTTAGAATACTTAAAAGAAAAAGAAGAGTT
ATTCGTCTTCAAAGGATTTGCAGGCGCGGACCGCAATTACCGTCTACCAATCCAAGTTATTAATGAATATGCATGGCACA
ATTTATTTGTACATCAATTATTCATTCGTCCAACTGAAGAAGAATTAACGACTCATGAATCAGAATTCACAATTGTTTCT
GCACCAAATTTCAAAGCTGATCCAGCTGTTGACGGTACAAACTCTGAAGCATTCATTATGGTTTCATTCGAAAAACGTAT
CGTACTAATTGGTGGTACAGAATACGCTGGAGAAATGAAAAAATCAATCTTCTCTATTATGAACTTCTTACTACCTGAAC
AAGATATTCTTTCTATGCATTGCTCTGCAAACGTAGGTGAAGAAGGCGACGTAGCACTATTCTTCGGTTTATCTGGAACA
GGTAAAACAACATTATCTGCTGATCCAAACCGTAAATTAATCGGTGATGATGAGCATGGTTGGTCTGATAACGGTGTATT
CAATATTGAAGGCGGTTGCTACGCAAAATGTGTAAACCTTTCTCACGAGAAAGAACCACAAATTTTCGATGCAATCACAT
TTGGATCTGTTTTAGAAAACGTTATCATTAATGACCAAACGCGTATCGCAGACTATAACGATACTACTTTAACAGAAAAT
ACTCGTGCTGCATACCCTATGCATGCGATCGACAATATCATACTGCCAAGTGTTGCTGGACACCCAAATACAATTATTTT
CTTAACTGCTGATGCATCTGGCGTATTGCCTCCAATCAGCAAGTTATCAAAAGAACAAGCTATGTACCATTTCTTAAGTG
GTTACACTAGTAAACTAGCAGGAACAGAGCGCGGCGTTACATCACCGCAAGCAACATTCTCAACTTGCTTCGGTTCACCA
TTCTTACCACTTGATGCATCTCGCTATGCTGAAATGCTTGGTGAGAAAATCGAGAAACACGATGCAAAAGTATTCTTAGT
AAACACTGGCTGGACTGGTGGCGAATACGGCGTTGGTAAACGTATGAACTTAGGTTACACTCGTGCAATGATTCAAGCAG
CATTAAACGGTGAACTTGCGAAAACTGAAACAGCTAAACATGACATCTTCGGTCTTGAAGTTCCACTTCACGTACCAGGT
GTACCTGACGAAGTGTTAATGCCTGAACAAACTTGGGCTGATAAAGCTGCTTACAAAGCAAAAGCAATCGAACTTGCAAA
TGAATTCAAAGCGAACTTCAAAAAGTTCGACAGCGTATCTGAAGACATTATTAACTTAGGCGGTCCAATCGCTTAA

Upstream 100 bases:

>100_bases
GCCGTTTCTGCTTCAACTGGAAAATATACAGGACGTTCTCCTAAAGATAAATTTATTGTGAAAGAAGCATCGGTTGCTGA
CAAAATTGCTTGGGGGAGCT

Downstream 100 bases:

>100_bases
GTGCAAACTTTCTGTTGGAATAACCCTTTACTCATATGAATAAGTGTCGTGTAACCGTAAAGTTTCGCGTACTCACCGAC
AAAACCCAATGCGGCTTAAC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNQPISEEHFNKLYTKVLEYLKEKEELFVFKGFAGADRNYRLPIQVINEYAWHNLFVHQLFIRPTEEELTTHESEFTIVS
APNFKADPAVDGTNSEAFIMVSFEKRIVLIGGTEYAGEMKKSIFSIMNFLLPEQDILSMHCSANVGEEGDVALFFGLSGT
GKTTLSADPNRKLIGDDEHGWSDNGVFNIEGGCYAKCVNLSHEKEPQIFDAITFGSVLENVIINDQTRIADYNDTTLTEN
TRAAYPMHAIDNIILPSVAGHPNTIIFLTADASGVLPPISKLSKEQAMYHFLSGYTSKLAGTERGVTSPQATFSTCFGSP
FLPLDASRYAEMLGEKIEKHDAKVFLVNTGWTGGEYGVGKRMNLGYTRAMIQAALNGELAKTETAKHDIFGLEVPLHVPG
VPDEVLMPEQTWADKAAYKAKAIELANEFKANFKKFDSVSEDIINLGGPIA

Sequences:

>Translated_451_residues
MNQPISEEHFNKLYTKVLEYLKEKEELFVFKGFAGADRNYRLPIQVINEYAWHNLFVHQLFIRPTEEELTTHESEFTIVS
APNFKADPAVDGTNSEAFIMVSFEKRIVLIGGTEYAGEMKKSIFSIMNFLLPEQDILSMHCSANVGEEGDVALFFGLSGT
GKTTLSADPNRKLIGDDEHGWSDNGVFNIEGGCYAKCVNLSHEKEPQIFDAITFGSVLENVIINDQTRIADYNDTTLTEN
TRAAYPMHAIDNIILPSVAGHPNTIIFLTADASGVLPPISKLSKEQAMYHFLSGYTSKLAGTERGVTSPQATFSTCFGSP
FLPLDASRYAEMLGEKIEKHDAKVFLVNTGWTGGEYGVGKRMNLGYTRAMIQAALNGELAKTETAKHDIFGLEVPLHVPG
VPDEVLMPEQTWADKAAYKAKAIELANEFKANFKKFDSVSEDIINLGGPIA
>Mature_451_residues
MNQPISEEHFNKLYTKVLEYLKEKEELFVFKGFAGADRNYRLPIQVINEYAWHNLFVHQLFIRPTEEELTTHESEFTIVS
APNFKADPAVDGTNSEAFIMVSFEKRIVLIGGTEYAGEMKKSIFSIMNFLLPEQDILSMHCSANVGEEGDVALFFGLSGT
GKTTLSADPNRKLIGDDEHGWSDNGVFNIEGGCYAKCVNLSHEKEPQIFDAITFGSVLENVIINDQTRIADYNDTTLTEN
TRAAYPMHAIDNIILPSVAGHPNTIIFLTADASGVLPPISKLSKEQAMYHFLSGYTSKLAGTERGVTSPQATFSTCFGSP
FLPLDASRYAEMLGEKIEKHDAKVFLVNTGWTGGEYGVGKRMNLGYTRAMIQAALNGELAKTETAKHDIFGLEVPLHVPG
VPDEVLMPEQTWADKAAYKAKAIELANEFKANFKKFDSVSEDIINLGGPIA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=441, Percent_Identity=50.7936507936508, Blast_Score=440, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6322950, Length=454, Percent_Identity=51.3215859030837, Blast_Score=468, Evalue=1e-133,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 49846; Mature: 49846

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQPISEEHFNKLYTKVLEYLKEKEELFVFKGFAGADRNYRLPIQVINEYAWHNLFVHQL
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHEEHHH
FIRPTEEELTTHESEFTIVSAPNFKADPAVDGTNSEAFIMVSFEKRIVLIGGTEYAGEMK
EECCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEEECCHHHHHHH
KSIFSIMNFLLPEQDILSMHCSANVGEEGDVALFFGLSGTGKTTLSADPNRKLIGDDEHG
HHHHHHHHHHCCCHHHHHEECCCCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCCC
WSDNGVFNIEGGCYAKCVNLSHEKEPQIFDAITFGSVLENVIINDQTRIADYNDTTLTEN
CCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
TRAAYPMHAIDNIILPSVAGHPNTIIFLTADASGVLPPISKLSKEQAMYHFLSGYTSKLA
CCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GTERGVTSPQATFSTCFGSPFLPLDASRYAEMLGEKIEKHDAKVFLVNTGWTGGEYGVGK
CCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
RMNLGYTRAMIQAALNGELAKTETAKHDIFGLEVPLHVPGVPDEVLMPEQTWADKAAYKA
CCCCHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEEEECCCCCHHHCCCCHHHHHHHHHHH
KAIELANEFKANFKKFDSVSEDIINLGGPIA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNQPISEEHFNKLYTKVLEYLKEKEELFVFKGFAGADRNYRLPIQVINEYAWHNLFVHQL
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHEEHHH
FIRPTEEELTTHESEFTIVSAPNFKADPAVDGTNSEAFIMVSFEKRIVLIGGTEYAGEMK
EECCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEEECCHHHHHHH
KSIFSIMNFLLPEQDILSMHCSANVGEEGDVALFFGLSGTGKTTLSADPNRKLIGDDEHG
HHHHHHHHHHCCCHHHHHEECCCCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCCC
WSDNGVFNIEGGCYAKCVNLSHEKEPQIFDAITFGSVLENVIINDQTRIADYNDTTLTEN
CCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
TRAAYPMHAIDNIILPSVAGHPNTIIFLTADASGVLPPISKLSKEQAMYHFLSGYTSKLA
CCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GTERGVTSPQATFSTCFGSPFLPLDASRYAEMLGEKIEKHDAKVFLVNTGWTGGEYGVGK
CCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
RMNLGYTRAMIQAALNGELAKTETAKHDIFGLEVPLHVPGVPDEVLMPEQTWADKAAYKA
CCCCHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEEEECCCCCHHHCCCCHHHHHHHHHHH
KAIELANEFKANFKKFDSVSEDIINLGGPIA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA