Click here to switch to the map view.

The map label for this gene is bmr3 [H]

Identifier: 227817500

GI number: 227817500

Start: 4507732

End: 4509135

Strand: Direct

Name: bmr3 [H]

Synonym: BAMEG_4992

Alternate gene names: 227817500

Gene position: 4507732-4509135 (Clockwise)

Preceding gene: 227817499

Following gene: 227817503

Centisome position: 86.19

GC content: 39.39

Gene sequence:

>1404_bases
ATGACGTTTCTTTCTGCGGTAGAAGGAACGATTGTTAGTACAGCAATCCCTCGTATAACGAGTGATTTGTCAGGCGTTGA
ACTTGTTAGCTGGGTTTACGCAATTTACATGCTTGCCACAGCGGTTTCAACTCCAATATACGGAAAGCTAGCTGATTTAT
TTGGCCGTAAAAAAGTTTTACTTATTGGAGCTACAATCTTTTTAGTCGGCTCTGCACTTTGCGGTGTCGTTACATCAATG
GAACAATTAATCTTCTTCCGCGCACTTCAAGGTATCGGTGCTGGTGCTGTTATGCCGATCACAATGACGATTATTGGAGA
CTTATATAGCGAAGCGAAAGACCGCGCGAAAGCACAAGGCTGGATGAGTGCTGTTTGGGGCGTCTCAGGTGTTATCGGAC
CATTAGTCGGCGGATTTTTAGTTGATTCTCTTTCTTGGCGTTATATTTTCTTCTTAAACGTTCCATTTGGAATTATCGCT
TGCTTAATGATTGCAATTTATTACAAAGAATCTATTAAGCCTGCTAAGCACCATATCGACTATCTTGGTGCAACAGTATT
CTCACTAAGTATGATTGCTCTACTATACGCATTATTAACAGGTAGCAGTAAGCAAAACTGGGGAGATGTAACAATTATCG
GCCTATTAATCTTCGCCGTCGTTTCATTCATTATTTTCTTATTCATCGAGAAAAAATCTCCGGAACCATTAATTCCGCTA
GCACTTTTCTCTAACCGTACATTATCTACGATAAATATATTAACGCTTATCGCTGGCGCAATGATTATTAGTATTACAAT
GTACCTTCCAATTTGGAGCCAAGGTGTATTAGGGAAAAATGCGACAGAAGCCGGACTTATTTTAATGCCAATCCCTGTTA
TGTGGACATTTGGTGCTATTTTCTCCGGTAACTTAGTCGGCAAATTAAAAACGAAACAAATCATTTTACTTGGAGCTAGC
ATTTTATCAGTTGCTACGTTCTTATTATTTACATTATCAACAAATTCACCATCGTTCCTTATTTATGTAGCAGTCGGATT
ATTTGGCTTAGGAATGGGACTTGTGACACCGATTTATATGGTAACAATTCAAGCAGCCGTACCAGCTCATACACGCGGAA
CAGCCGTTGGTTTAAACACATTTATTAATACATTTAGCCAAACATTAGGTGCGGCAGTATTCGGTACAATCTTTAATACG
ATGATTCACGCACGTGGCATTAAAAACCTTGATCTTGTATCTGGCGGACACGGCGGAGCTACAGCAAACGTAGTAACTGA
ATCACAATCTGCATTAGCATCAAGTGTACACGTTATTTATATGAGCACTTTCGTATTAGCAGTATGTACATTAATTATCG
CTTGGCTTTTATTAAAGCCCGCTACACAAACAAGTGAGCAATAA

Upstream 100 bases:

>100_bases
TTTATATACCTTAGTACCTTTATATTTTATATTGTTGAATATTTGAAAAAGGGTAAGGGGAAAACATATGAGAAAAAAAG
TCATGATGTCTTTAATGCTA

Downstream 100 bases:

>100_bases
TAAAGAGGATGACCATTTCGGTCATCCTCTTTTACTCTTCATTCTCCACAATATGTTTCAGCATGGACAATTTATTATCC
CAAAAACGTTCATAATACGA

Product: drug resistance transporter, EmrB/QacA family

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTFLSAVEGTIVSTAIPRITSDLSGVELVSWVYAIYMLATAVSTPIYGKLADLFGRKKVLLIGATIFLVGSALCGVVTSM
EQLIFFRALQGIGAGAVMPITMTIIGDLYSEAKDRAKAQGWMSAVWGVSGVIGPLVGGFLVDSLSWRYIFFLNVPFGIIA
CLMIAIYYKESIKPAKHHIDYLGATVFSLSMIALLYALLTGSSKQNWGDVTIIGLLIFAVVSFIIFLFIEKKSPEPLIPL
ALFSNRTLSTINILTLIAGAMIISITMYLPIWSQGVLGKNATEAGLILMPIPVMWTFGAIFSGNLVGKLKTKQIILLGAS
ILSVATFLLFTLSTNSPSFLIYVAVGLFGLGMGLVTPIYMVTIQAAVPAHTRGTAVGLNTFINTFSQTLGAAVFGTIFNT
MIHARGIKNLDLVSGGHGGATANVVTESQSALASSVHVIYMSTFVLAVCTLIIAWLLLKPATQTSEQ

Sequences:

>Translated_467_residues
MTFLSAVEGTIVSTAIPRITSDLSGVELVSWVYAIYMLATAVSTPIYGKLADLFGRKKVLLIGATIFLVGSALCGVVTSM
EQLIFFRALQGIGAGAVMPITMTIIGDLYSEAKDRAKAQGWMSAVWGVSGVIGPLVGGFLVDSLSWRYIFFLNVPFGIIA
CLMIAIYYKESIKPAKHHIDYLGATVFSLSMIALLYALLTGSSKQNWGDVTIIGLLIFAVVSFIIFLFIEKKSPEPLIPL
ALFSNRTLSTINILTLIAGAMIISITMYLPIWSQGVLGKNATEAGLILMPIPVMWTFGAIFSGNLVGKLKTKQIILLGAS
ILSVATFLLFTLSTNSPSFLIYVAVGLFGLGMGLVTPIYMVTIQAAVPAHTRGTAVGLNTFINTFSQTLGAAVFGTIFNT
MIHARGIKNLDLVSGGHGGATANVVTESQSALASSVHVIYMSTFVLAVCTLIIAWLLLKPATQTSEQ
>Mature_466_residues
TFLSAVEGTIVSTAIPRITSDLSGVELVSWVYAIYMLATAVSTPIYGKLADLFGRKKVLLIGATIFLVGSALCGVVTSME
QLIFFRALQGIGAGAVMPITMTIIGDLYSEAKDRAKAQGWMSAVWGVSGVIGPLVGGFLVDSLSWRYIFFLNVPFGIIAC
LMIAIYYKESIKPAKHHIDYLGATVFSLSMIALLYALLTGSSKQNWGDVTIIGLLIFAVVSFIIFLFIEKKSPEPLIPLA
LFSNRTLSTINILTLIAGAMIISITMYLPIWSQGVLGKNATEAGLILMPIPVMWTFGAIFSGNLVGKLKTKQIILLGASI
LSVATFLLFTLSTNSPSFLIYVAVGLFGLGMGLVTPIYMVTIQAAVPAHTRGTAVGLNTFINTFSQTLGAAVFGTIFNTM
IHARGIKNLDLVSGGHGGATANVVTESQSALASSVHVIYMSTFVLAVCTLIIAWLLLKPATQTSEQ

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=424, Percent_Identity=26.4150943396226, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI87081983, Length=400, Percent_Identity=28, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788710, Length=492, Percent_Identity=25.2032520325203, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1790195, Length=362, Percent_Identity=27.9005524861878, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1788392, Length=294, Percent_Identity=28.9115646258503, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790146, Length=161, Percent_Identity=27.9503105590062, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788509, Length=151, Percent_Identity=29.8013245033113, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI48994889, Length=202, Percent_Identity=29.7029702970297, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=459, Percent_Identity=24.6187363834423, Blast_Score=123, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6323735, Length=301, Percent_Identity=27.5747508305648, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6319770, Length=368, Percent_Identity=26.3586956521739, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319783, Length=382, Percent_Identity=24.0837696335079, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6321663, Length=463, Percent_Identity=22.6781857451404, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324954, Length=410, Percent_Identity=22.6829268292683, Blast_Score=73, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=141, Percent_Identity=29.7872340425532, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323520, Length=292, Percent_Identity=26.7123287671233, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49777; Mature: 49646

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFLSAVEGTIVSTAIPRITSDLSGVELVSWVYAIYMLATAVSTPIYGKLADLFGRKKVL
CCCHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEE
LIGATIFLVGSALCGVVTSMEQLIFFRALQGIGAGAVMPITMTIIGDLYSEAKDRAKAQG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WMSAVWGVSGVIGPLVGGFLVDSLSWRYIFFLNVPFGIIACLMIAIYYKESIKPAKHHID
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHH
YLGATVFSLSMIALLYALLTGSSKQNWGDVTIIGLLIFAVVSFIIFLFIEKKSPEPLIPL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEH
ALFSNRTLSTINILTLIAGAMIISITMYLPIWSQGVLGKNATEAGLILMPIPVMWTFGAI
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHH
FSGNLVGKLKTKQIILLGASILSVATFLLFTLSTNSPSFLIYVAVGLFGLGMGLVTPIYM
HCCCCEEEECCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
VTIQAAVPAHTRGTAVGLNTFINTFSQTLGAAVFGTIFNTMIHARGIKNLDLVSGGHGGA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCC
TANVVTESQSALASSVHVIYMSTFVLAVCTLIIAWLLLKPATQTSEQ
EEHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TFLSAVEGTIVSTAIPRITSDLSGVELVSWVYAIYMLATAVSTPIYGKLADLFGRKKVL
CCHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEE
LIGATIFLVGSALCGVVTSMEQLIFFRALQGIGAGAVMPITMTIIGDLYSEAKDRAKAQG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WMSAVWGVSGVIGPLVGGFLVDSLSWRYIFFLNVPFGIIACLMIAIYYKESIKPAKHHID
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHH
YLGATVFSLSMIALLYALLTGSSKQNWGDVTIIGLLIFAVVSFIIFLFIEKKSPEPLIPL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEH
ALFSNRTLSTINILTLIAGAMIISITMYLPIWSQGVLGKNATEAGLILMPIPVMWTFGAI
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHH
FSGNLVGKLKTKQIILLGASILSVATFLLFTLSTNSPSFLIYVAVGLFGLGMGLVTPIYM
HCCCCEEEECCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
VTIQAAVPAHTRGTAVGLNTFINTFSQTLGAAVFGTIFNTMIHARGIKNLDLVSGGHGGA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCC
TANVVTESQSALASSVHVIYMSTFVLAVCTLIIAWLLLKPATQTSEQ
EEHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]