| Definition | Bacillus anthracis str. CDC 684, complete genome. |
|---|---|
| Accession | NC_012581 |
| Length | 5,230,115 |
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The map label for this gene is amyX [H]
Identifier: 227817491
GI number: 227817491
Start: 4498471
End: 4500612
Strand: Reverse
Name: amyX [H]
Synonym: BAMEG_4983
Alternate gene names: 227817491
Gene position: 4500612-4498471 (Counterclockwise)
Preceding gene: 227817492
Following gene: 227817490
Centisome position: 86.05
GC content: 37.54
Gene sequence:
>2142_bases ATGTTGAAAGTAAAACGTCCATTTGATGCCTATTTAGATGAAATGAATAAAATTACAATTTTGCTCCCACATGCGTATGG AACAAGTCGGACATTTCGTTTGCAAGAAGGAAGTAACGTGAAAGATTTACCGATTGCTCATACAATTGCGCTTCCGGATG CAACGAAGTATGAGTGCTTTATAGAAGAGCCGCTAGATGTGGGGAAGTATTATACAGTACGAGATGAACGGAATGAAGAA ACAGATTTACAAATAGGTGCTGTCATAAGAACAGAGATTTTCGATGAAAAGTATTATTACGAAGGTACAGATTTAGGGGC TGTATATCAAAAAGAAGCAACTACATTTAAAGTATGGGCTCCAACTGCGAGGCTTGCGAAAGTAAGAATTTATAAAAGTG ATAAAGAATATACCGATCACGAAATGCACAGAGAAGAAAATGGGGTATGGGCTCATACATTACAAGGCGATTTAGCCGGG GAAAAGTATACATTTCTTGTTTGTATTAATTTAATATGGAATGAAGCTGTGGATCCTTATGCAAAATCAGTGACTGTAAA TGGGAAGTACGGCGTTGTTATCGATTTGGAAAAAACAAATGTAACAAAGCAAGAGCAATTACCACCATTACAAGCAATGA CAGATGCTATATTGTATGAACTGCATATTCGTGATGCTACTATTCATGAGGGCAGTGGAGTGAGTAAAAAAGGAACGTAT AAAGGGCTAATGGAAGAAGGAACAACAGGGCGAAATGGGACGTTAACAGGTTTGTCTCATATAAAAGATTTAGGTGTTAC ACATGTTGAATTATTGCCTTTATATTGTTTTGGTGGTGTAGATGAGGCGAATCCTTCTTCTGCATATAATTGGGGTTACA ATCCATTATATTACAACGCGCCAACGGGGTTTTATGCTACAAACCCATCTGACCCGTATAACAGGATCTTAGAGTGTAAG CAACTGATTGAAACATTTCATGAACACGGCATTAGGGTGATTATAGATGTTGTATATAATCATGTGTATGAAAGAGAGCT ATCATCATTTGAAAAGCTTGTCCCAGGATATTATTTTCGTCATGGTGAAAATGGTATGCCTTCTAATGGGACGGGAGTCG GAAATGATATAGCATCTGAACGGAAAATGATGAGGAAATTCATTGTAGAGTCTATTTTATATTGGCTTACGGAATACAAT GTTGATGGATTCCGGTTCGACTTAATGGGGATTTTAGATGTCGATACAATCAATATAATAGAAAAAGAAGTGCGAAACAT AAAGCGAGATGCATTGCTATTAGGAGAAGGGTGGGATCTGCAAACACCACTTCCTCTAGAGGAAAAAGCAACGTTAAATA ATGCAAACAAAATGCCGCATATTGCGCAATTTAATGATCAATTTCGTGATGGGATAAAAGGGAGTACCTTTCATATAAAT AAACGTGGTTTTGCATTTGGTGGGCATGTAGACCGTAATCATTTGCGGTATATAGCATCAGGAAGCCTTTTGAGTATGAA AGAAACAGGTTTGTTTCTAGAGCCTGTGCAAAGCATTAACTATGTAGAATGTCATGACAATATGACGATGTGGGATAAAC TAATGCGCAGTAATGAAGAGTCGGAAGAGATTTTGAAAAAACGTCACGTGCTAGCAACTGCAATGGTGATTCTGTCGCAA GGCATCCCTTTCTTACATGCGGGGCAAGAGTTTTATCGTACGAAGCAAGGGAATGAAAATAGTTATAATGCAAATGATGA AATTAATCAATTAGATTGGGATCGAAAAGAGAAAGAAATAGAGACCGTTAACTATATAAAAGGTTTAATTGCGATTCGTA AAGAGCACGGAGCATTCCGATTACAAAACGCAGACTTAATAAAAAAGCACATGACTTTTTTGCAAACATCTCCAGAAGTA CTTGCATATCACTTAGAGCACGTAGAATCGTTTGGACCGTGGAAAGAGATTGTCGTTTTATTTAATAGCAGTTTAGAAGC GAAAACGGTGCAACTTCCGAAAGAAGAAACGTGGCATGTGTTAGTAAACGAAAAGCAAGCGAAAATAGAGCCAATTTCTT CATTTAGAGGAAAGGAACTTAGGCTCTCTCCAATTAGCACGTATATATTGACGATAATGTGA
Upstream 100 bases:
>100_bases ATAAAGAATAATGTGTTACTGTATAGGGCTTCGCTGATAAGAAAAGCCTTATCATTTGCAGCTCGTTTCATTTTAACTTT AAGAATGATGGTGATTTTTT
Downstream 100 bases:
>100_bases CAAATCGCTACTTGACGGTGAGAATGTAATAAATGTAGAATTGACAAAGGTGACGATTTTGATAAGTTGTCATTCTATGG CTGGTTTTGTAAAATAAAAT
Product: putative pullulanase
Products: NA
Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]
Number of amino acids: Translated: 713; Mature: 713
Protein sequence:
>713_residues MLKVKRPFDAYLDEMNKITILLPHAYGTSRTFRLQEGSNVKDLPIAHTIALPDATKYECFIEEPLDVGKYYTVRDERNEE TDLQIGAVIRTEIFDEKYYYEGTDLGAVYQKEATTFKVWAPTARLAKVRIYKSDKEYTDHEMHREENGVWAHTLQGDLAG EKYTFLVCINLIWNEAVDPYAKSVTVNGKYGVVIDLEKTNVTKQEQLPPLQAMTDAILYELHIRDATIHEGSGVSKKGTY KGLMEEGTTGRNGTLTGLSHIKDLGVTHVELLPLYCFGGVDEANPSSAYNWGYNPLYYNAPTGFYATNPSDPYNRILECK QLIETFHEHGIRVIIDVVYNHVYERELSSFEKLVPGYYFRHGENGMPSNGTGVGNDIASERKMMRKFIVESILYWLTEYN VDGFRFDLMGILDVDTINIIEKEVRNIKRDALLLGEGWDLQTPLPLEEKATLNNANKMPHIAQFNDQFRDGIKGSTFHIN KRGFAFGGHVDRNHLRYIASGSLLSMKETGLFLEPVQSINYVECHDNMTMWDKLMRSNEESEEILKKRHVLATAMVILSQ GIPFLHAGQEFYRTKQGNENSYNANDEINQLDWDRKEKEIETVNYIKGLIAIRKEHGAFRLQNADLIKKHMTFLQTSPEV LAYHLEHVESFGPWKEIVVLFNSSLEAKTVQLPKEETWHVLVNEKQAKIEPISSFRGKELRLSPISTYILTIM
Sequences:
>Translated_713_residues MLKVKRPFDAYLDEMNKITILLPHAYGTSRTFRLQEGSNVKDLPIAHTIALPDATKYECFIEEPLDVGKYYTVRDERNEE TDLQIGAVIRTEIFDEKYYYEGTDLGAVYQKEATTFKVWAPTARLAKVRIYKSDKEYTDHEMHREENGVWAHTLQGDLAG EKYTFLVCINLIWNEAVDPYAKSVTVNGKYGVVIDLEKTNVTKQEQLPPLQAMTDAILYELHIRDATIHEGSGVSKKGTY KGLMEEGTTGRNGTLTGLSHIKDLGVTHVELLPLYCFGGVDEANPSSAYNWGYNPLYYNAPTGFYATNPSDPYNRILECK QLIETFHEHGIRVIIDVVYNHVYERELSSFEKLVPGYYFRHGENGMPSNGTGVGNDIASERKMMRKFIVESILYWLTEYN VDGFRFDLMGILDVDTINIIEKEVRNIKRDALLLGEGWDLQTPLPLEEKATLNNANKMPHIAQFNDQFRDGIKGSTFHIN KRGFAFGGHVDRNHLRYIASGSLLSMKETGLFLEPVQSINYVECHDNMTMWDKLMRSNEESEEILKKRHVLATAMVILSQ GIPFLHAGQEFYRTKQGNENSYNANDEINQLDWDRKEKEIETVNYIKGLIAIRKEHGAFRLQNADLIKKHMTFLQTSPEV LAYHLEHVESFGPWKEIVVLFNSSLEAKTVQLPKEETWHVLVNEKQAKIEPISSFRGKELRLSPISTYILTIM >Mature_713_residues MLKVKRPFDAYLDEMNKITILLPHAYGTSRTFRLQEGSNVKDLPIAHTIALPDATKYECFIEEPLDVGKYYTVRDERNEE TDLQIGAVIRTEIFDEKYYYEGTDLGAVYQKEATTFKVWAPTARLAKVRIYKSDKEYTDHEMHREENGVWAHTLQGDLAG EKYTFLVCINLIWNEAVDPYAKSVTVNGKYGVVIDLEKTNVTKQEQLPPLQAMTDAILYELHIRDATIHEGSGVSKKGTY KGLMEEGTTGRNGTLTGLSHIKDLGVTHVELLPLYCFGGVDEANPSSAYNWGYNPLYYNAPTGFYATNPSDPYNRILECK QLIETFHEHGIRVIIDVVYNHVYERELSSFEKLVPGYYFRHGENGMPSNGTGVGNDIASERKMMRKFIVESILYWLTEYN VDGFRFDLMGILDVDTINIIEKEVRNIKRDALLLGEGWDLQTPLPLEEKATLNNANKMPHIAQFNDQFRDGIKGSTFHIN KRGFAFGGHVDRNHLRYIASGSLLSMKETGLFLEPVQSINYVECHDNMTMWDKLMRSNEESEEILKKRHVLATAMVILSQ GIPFLHAGQEFYRTKQGNENSYNANDEINQLDWDRKEKEIETVNYIKGLIAIRKEHGAFRLQNADLIKKHMTFLQTSPEV LAYHLEHVESFGPWKEIVVLFNSSLEAKTVQLPKEETWHVLVNEKQAKIEPISSFRGKELRLSPISTYILTIM
Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]
COG id: COG1523
COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 13 family [H]
Homologues:
Organism=Escherichia coli, GI2367229, Length=669, Percent_Identity=23.4678624813154, Blast_Score=144, Evalue=3e-35,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006047 - InterPro: IPR004193 - InterPro: IPR017853 - InterPro: IPR013781 - InterPro: IPR013783 - InterPro: IPR011840 [H]
Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]
EC number: =3.2.1.41 [H]
Molecular weight: Translated: 81733; Mature: 81733
Theoretical pI: Translated: 5.93; Mature: 5.93
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 3.1 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 3.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLKVKRPFDAYLDEMNKITILLPHAYGTSRTFRLQEGSNVKDLPIAHTIALPDATKYECF CCCCCCCHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCEEEE IEEPLDVGKYYTVRDERNEETDLQIGAVIRTEIFDEKYYYEGTDLGAVYQKEATTFKVWA ECCCCCCCCEEEECCCCCCCCCEEEHHEEEHHHHCCCEEECCCCCCCEEECCCCEEEEEC PTARLAKVRIYKSDKEYTDHEMHREENGVWAHTLQGDLAGEKYTFLVCINLIWNEAVDPY CCCEEEEEEEEECCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEEHHHHHCCCCH AKSVTVNGKYGVVIDLEKTNVTKQEQLPPLQAMTDAILYELHIRDATIHEGSGVSKKGTY HHEEEECCCEEEEEEEECCCCCHHHCCCCHHHHHHHHEEEEEECCEEEECCCCCCCCCCH KGLMEEGTTGRNGTLTGLSHIKDLGVTHVELLPLYCFGGVDEANPSSAYNWGYNPLYYNA HHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEHHEECCCCCCCCCCCCCCCCCCEEECC PTGFYATNPSDPYNRILECKQLIETFHEHGIRVIIDVVYNHVYERELSSFEKLVPGYYFR CCCCEECCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEE HGENGMPSNGTGVGNDIASERKMMRKFIVESILYWLTEYNVDGFRFDLMGILDVDTINII CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHH EKEVRNIKRDALLLGEGWDLQTPLPLEEKATLNNANKMPHIAQFNDQFRDGIKGSTFHIN HHHHHHHHHCEEEEECCCCCCCCCCCCHHHCCCCCCCCCCHHHHCHHHHCCCCCCEEEEE KRGFAFGGHVDRNHLRYIASGSLLSMKETGLFLEPVQSINYVECHDNMTMWDKLMRSNEE CCCEEECCCCCCCCEEEEECCCEEEHHHCCCCHHHHCCCCEEEECCCHHHHHHHHHCCCH SEEILKKRHVLATAMVILSQGIPFLHAGQEFYRTKQGNENSYNANDEINQLDWDRKEKEI HHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH ETVNYIKGLIAIRKEHGAFRLQNADLIKKHMTFLQTSPEVLAYHLEHVESFGPWKEIVVL HHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHEE FNSSLEAKTVQLPKEETWHVLVNEKQAKIEPISSFRGKELRLSPISTYILTIM ECCCCCCEEEECCCCCCEEEEECCCCCCCCCHHHCCCCEEEECHHCEEEEEEC >Mature Secondary Structure MLKVKRPFDAYLDEMNKITILLPHAYGTSRTFRLQEGSNVKDLPIAHTIALPDATKYECF CCCCCCCHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCEEEE IEEPLDVGKYYTVRDERNEETDLQIGAVIRTEIFDEKYYYEGTDLGAVYQKEATTFKVWA ECCCCCCCCEEEECCCCCCCCCEEEHHEEEHHHHCCCEEECCCCCCCEEECCCCEEEEEC PTARLAKVRIYKSDKEYTDHEMHREENGVWAHTLQGDLAGEKYTFLVCINLIWNEAVDPY CCCEEEEEEEEECCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEEHHHHHCCCCH AKSVTVNGKYGVVIDLEKTNVTKQEQLPPLQAMTDAILYELHIRDATIHEGSGVSKKGTY HHEEEECCCEEEEEEEECCCCCHHHCCCCHHHHHHHHEEEEEECCEEEECCCCCCCCCCH KGLMEEGTTGRNGTLTGLSHIKDLGVTHVELLPLYCFGGVDEANPSSAYNWGYNPLYYNA HHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEHHEECCCCCCCCCCCCCCCCCCEEECC PTGFYATNPSDPYNRILECKQLIETFHEHGIRVIIDVVYNHVYERELSSFEKLVPGYYFR CCCCEECCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEE HGENGMPSNGTGVGNDIASERKMMRKFIVESILYWLTEYNVDGFRFDLMGILDVDTINII CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHH EKEVRNIKRDALLLGEGWDLQTPLPLEEKATLNNANKMPHIAQFNDQFRDGIKGSTFHIN HHHHHHHHHCEEEEECCCCCCCCCCCCHHHCCCCCCCCCCHHHHCHHHHCCCCCCEEEEE KRGFAFGGHVDRNHLRYIASGSLLSMKETGLFLEPVQSINYVECHDNMTMWDKLMRSNEE CCCEEECCCCCCCCEEEEECCCEEEHHHCCCCHHHHCCCCEEEECCCHHHHHHHHHCCCH SEEILKKRHVLATAMVILSQGIPFLHAGQEFYRTKQGNENSYNANDEINQLDWDRKEKEI HHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH ETVNYIKGLIAIRKEHGAFRLQNADLIKKHMTFLQTSPEVLAYHLEHVESFGPWKEIVVL HHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHEE FNSSLEAKTVQLPKEETWHVLVNEKQAKIEPISSFRGKELRLSPISTYILTIM ECCCCCCEEEECCCCCCEEEEECCCCCCCCCHHHCCCCEEEECHHCEEEEEEC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9387221; 9384377 [H]