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The map label for this gene is nifS [H]

Identifier: 227817211

GI number: 227817211

Start: 4240339

End: 4241475

Strand: Direct

Name: nifS [H]

Synonym: BAMEG_4697

Alternate gene names: 227817211

Gene position: 4240339-4241475 (Clockwise)

Preceding gene: 227817205

Following gene: 227817212

Centisome position: 81.08

GC content: 37.55

Gene sequence:

>1137_bases
ATGATATATCTTGACTATGCAGCTACTACACCTATGAGTGTGGAAGCATTACAAACATATACGAAAGCAGCATCGCAATA
CTTCGGAAACGAACAAAGTTTACACGATATTGGAGGAACAGCCTCTTCTTTATTACAAGTTTGCAGAAAAACATTTGCTG
ACATGATCGGTGGAAAGGAACAAGGGGTATTCTTCACGAGCGGCGGGTCAGAATCGAACTATCTTGCGATTCAATCCCTT
CTCAATGCCCAAAATAAGAAGCATATTATTACGACACCTATGGAACATGCATCCATTCGAAGTTATTTTCAATCTCTAAA
ATCACAAGGCTATACGATTACTGAAATTCCTGTTGATAAAAGTGGACTCATTCGTTTAGTAGATTTAGAAACAGCTATTA
CAGAAGATACTGTTCTAGCAAGTATACAGCACGGAAACTCTGAAATCGGAACCGTTCAAAATATCGCAGAGATCGGGGCA
CTTTTAAAAAAATATAACGTTTTATTTCATTCAGATTGTGTGCAAACATTTGGTAAACTTCCTATACATGTTTTTGAGAT
GGGGATTGATAGTCTTTCTGTTTCAGCACATAAAATATACGGACCAAAGGGTGTTGGCGCTTGCTATATAAATCCGCAAG
TTCGCTGGACACAAGTATTTCCGGAAACTTCTCACGAAAAAGGTTTTCGCCCTGGCACAGTAAACGTCCCTGGCATCGCA
TCTTTTTTAACAGCTGCTGAGAATATATTAAAAAATCAACAAGAAGAAAGTTTACGTTTTAAAGAGTTACGATCCTACTT
TTTAGAGCAAATACAAACACTTCCGCTAGAAATTGAAGTAGAAGGTCATTCTACTTCTTGTTTACCACATATTATTGGGG
TTACAATAAAAGGAATAGAAGGTCAGTATACAATGCTAGAATGTAATCGCCGCGGTATTGCCATTTCCACGGGAAGTGCT
TGCCAAGTCGGTAAACAAGAACCTTCGAAAACAATGCTTGCAATTGGAAAAACGTATGAAGAGGCAAAACAATATGTCCG
TTTCTCTTTCGGACAACAAACAACGAAAGATCAAATTGATACTACTATTCATGCACTACACACAATTGGAAATCAATTTT
ATAGAGGTGTTAAATGA

Upstream 100 bases:

>100_bases
AGTTTTAAAATAATGACAAGAGTTTTTTTATAATTCTTATTTTCACTATCATTTAAAAATAAAAGAAATGTAACTCTTAC
TAGAGAGAGGAACTTACATG

Downstream 100 bases:

>100_bases
TAATGAAACAAAATGAACAAAAAAAGATTTTAGGTGAAGAAAGACGACAACTTATCCTTCAGTGGCTTCTAGCTGCAAAT
GAACCGTTATCTGGGAATGA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MIYLDYAATTPMSVEALQTYTKAASQYFGNEQSLHDIGGTASSLLQVCRKTFADMIGGKEQGVFFTSGGSESNYLAIQSL
LNAQNKKHIITTPMEHASIRSYFQSLKSQGYTITEIPVDKSGLIRLVDLETAITEDTVLASIQHGNSEIGTVQNIAEIGA
LLKKYNVLFHSDCVQTFGKLPIHVFEMGIDSLSVSAHKIYGPKGVGACYINPQVRWTQVFPETSHEKGFRPGTVNVPGIA
SFLTAAENILKNQQEESLRFKELRSYFLEQIQTLPLEIEVEGHSTSCLPHIIGVTIKGIEGQYTMLECNRRGIAISTGSA
CQVGKQEPSKTMLAIGKTYEEAKQYVRFSFGQQTTKDQIDTTIHALHTIGNQFYRGVK

Sequences:

>Translated_378_residues
MIYLDYAATTPMSVEALQTYTKAASQYFGNEQSLHDIGGTASSLLQVCRKTFADMIGGKEQGVFFTSGGSESNYLAIQSL
LNAQNKKHIITTPMEHASIRSYFQSLKSQGYTITEIPVDKSGLIRLVDLETAITEDTVLASIQHGNSEIGTVQNIAEIGA
LLKKYNVLFHSDCVQTFGKLPIHVFEMGIDSLSVSAHKIYGPKGVGACYINPQVRWTQVFPETSHEKGFRPGTVNVPGIA
SFLTAAENILKNQQEESLRFKELRSYFLEQIQTLPLEIEVEGHSTSCLPHIIGVTIKGIEGQYTMLECNRRGIAISTGSA
CQVGKQEPSKTMLAIGKTYEEAKQYVRFSFGQQTTKDQIDTTIHALHTIGNQFYRGVK
>Mature_378_residues
MIYLDYAATTPMSVEALQTYTKAASQYFGNEQSLHDIGGTASSLLQVCRKTFADMIGGKEQGVFFTSGGSESNYLAIQSL
LNAQNKKHIITTPMEHASIRSYFQSLKSQGYTITEIPVDKSGLIRLVDLETAITEDTVLASIQHGNSEIGTVQNIAEIGA
LLKKYNVLFHSDCVQTFGKLPIHVFEMGIDSLSVSAHKIYGPKGVGACYINPQVRWTQVFPETSHEKGFRPGTVNVPGIA
SFLTAAENILKNQQEESLRFKELRSYFLEQIQTLPLEIEVEGHSTSCLPHIIGVTIKGIEGQYTMLECNRRGIAISTGSA
CQVGKQEPSKTMLAIGKTYEEAKQYVRFSFGQQTTKDQIDTTIHALHTIGNQFYRGVK

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Seems to be required for NAD biosynthesis [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=368, Percent_Identity=35.8695652173913, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI156713448, Length=406, Percent_Identity=31.5270935960591, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI48994898, Length=369, Percent_Identity=35.7723577235772, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1787970, Length=399, Percent_Identity=23.0576441102757, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25143064, Length=368, Percent_Identity=34.7826086956522, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17533177, Length=330, Percent_Identity=30.9090909090909, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319831, Length=370, Percent_Identity=35.4054054054054, Blast_Score=224, Evalue=1e-59,
Organism=Drosophila melanogaster, GI20129463, Length=366, Percent_Identity=34.4262295081967, Blast_Score=217, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41693; Mature: 41693

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDYAATTPMSVEALQTYTKAASQYFGNEQSLHDIGGTASSLLQVCRKTFADMIGGKE
CEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCC
QGVFFTSGGSESNYLAIQSLLNAQNKKHIITTPMEHASIRSYFQSLKSQGYTITEIPVDK
CCEEEECCCCCCCHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHCCCEEEECCCCC
SGLIRLVDLETAITEDTVLASIQHGNSEIGTVQNIAEIGALLKKYNVLFHSDCVQTFGKL
CCCEEEEECHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PIHVFEMGIDSLSVSAHKIYGPKGVGACYINPQVRWTQVFPETSHEKGFRPGTVNVPGIA
CEEEEECCCCHHCCCHHEEECCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCHHH
SFLTAAENILKNQQEESLRFKELRSYFLEQIQTLPLEIEVEGHSTSCLPHIIGVTIKGIE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCEEEECCC
GQYTMLECNRRGIAISTGSACQVGKQEPSKTMLAIGKTYEEAKQYVRFSFGQQTTKDQID
CCEEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHH
TTIHALHTIGNQFYRGVK
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIYLDYAATTPMSVEALQTYTKAASQYFGNEQSLHDIGGTASSLLQVCRKTFADMIGGKE
CEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCC
QGVFFTSGGSESNYLAIQSLLNAQNKKHIITTPMEHASIRSYFQSLKSQGYTITEIPVDK
CCEEEECCCCCCCHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHCCCEEEECCCCC
SGLIRLVDLETAITEDTVLASIQHGNSEIGTVQNIAEIGALLKKYNVLFHSDCVQTFGKL
CCCEEEEECHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PIHVFEMGIDSLSVSAHKIYGPKGVGACYINPQVRWTQVFPETSHEKGFRPGTVNVPGIA
CEEEEECCCCHHCCCHHEEECCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCHHH
SFLTAAENILKNQQEESLRFKELRSYFLEQIQTLPLEIEVEGHSTSCLPHIIGVTIKGIE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCEEEECCC
GQYTMLECNRRGIAISTGSACQVGKQEPSKTMLAIGKTYEEAKQYVRFSFGQQTTKDQID
CCEEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHH
TTIHALHTIGNQFYRGVK
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8444804; 9384377 [H]