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The map label for this gene is ykwC [H]

Identifier: 227816821

GI number: 227816821

Start: 3899026

End: 3899388

Strand: Reverse

Name: ykwC [H]

Synonym: BAMEG_4303

Alternate gene names: 227816821

Gene position: 3899388-3899026 (Counterclockwise)

Preceding gene: 227816822

Following gene: 227816820

Centisome position: 74.56

GC content: 34.16

Gene sequence:

>363_bases
ATGGAACATAAAACTTTATCAATAGGTTTCATCGGTATTGGTGTAATGGGAAAAAGTATGGTTCATCATTTAATGCAAGA
AGGTCATAAGGTATATGTATATAATAGAACGAAAGCGAAAACAGATTCTTTAGTGCAAGATGGTGCAAATTGGTGTGATA
CACCGAAAGACTTAGTAAAGCAAGTGGATGTTGTAATGACTATGGTAGGATATCCACATGATGTAGAAGAAGTGTATTTT
GGGATAGATGGGATTTTGGAGAATGCAAATGAAGGTACAATTACAATTGATTTCACGACATCTACACCAACATTGGCAAA
ACGTATTAATGAAGCGGGTAAAAGTAAGAATATATATCGTTAG

Upstream 100 bases:

>100_bases
ATGTAATGAGAAGTTTTGGTTGGAGATAAAGAACGTGTTATATTGAATAAAGAGTTCTGAAATATAGTAAGAGAGTTATT
AGAACAAAGGGGATGAGAAA

Downstream 100 bases:

>100_bases
ATGCGCCTGTATCTGGAGGAGACGTCGGTGCGAAAGAGGCAAGACTCGCAATTATGGTCGGTGGCGAGAAAGAAATATAT
GAAAAATGTTTACCGTTATT

Product: 2-hydroxy-3-oxopropionate reductase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MEHKTLSIGFIGIGVMGKSMVHHLMQEGHKVYVYNRTKAKTDSLVQDGANWCDTPKDLVKQVDVVMTMVGYPHDVEEVYF
GIDGILENANEGTITIDFTTSTPTLAKRINEAGKSKNIYR

Sequences:

>Translated_120_residues
MEHKTLSIGFIGIGVMGKSMVHHLMQEGHKVYVYNRTKAKTDSLVQDGANWCDTPKDLVKQVDVVMTMVGYPHDVEEVYF
GIDGILENANEGTITIDFTTSTPTLAKRINEAGKSKNIYR
>Mature_120_residues
MEHKTLSIGFIGIGVMGKSMVHHLMQEGHKVYVYNRTKAKTDSLVQDGANWCDTPKDLVKQVDVVMTMVGYPHDVEEVYF
GIDGILENANEGTITIDFTTSTPTLAKRINEAGKSKNIYR

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=93, Percent_Identity=35.4838709677419, Blast_Score=73, Evalue=4e-14,
Organism=Homo sapiens, GI23308751, Length=102, Percent_Identity=29.4117647058824, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI145693186, Length=113, Percent_Identity=36.283185840708, Blast_Score=87, Evalue=3e-19,
Organism=Escherichia coli, GI1790315, Length=97, Percent_Identity=31.9587628865979, Blast_Score=63, Evalue=7e-12,
Organism=Escherichia coli, GI1786719, Length=112, Percent_Identity=30.3571428571429, Blast_Score=62, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28574115, Length=112, Percent_Identity=33.9285714285714, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 13391; Mature: 13391

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHKTLSIGFIGIGVMGKSMVHHLMQEGHKVYVYNRTKAKTDSLVQDGANWCDTPKDLVK
CCCCEEEEEEEEHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCCCHHHHHH
QVDVVMTMVGYPHDVEEVYFGIDGILENANEGTITIDFTTSTPTLAKRINEAGKSKNIYR
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEHKTLSIGFIGIGVMGKSMVHHLMQEGHKVYVYNRTKAKTDSLVQDGANWCDTPKDLVK
CCCCEEEEEEEEHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCCCHHHHHH
QVDVVMTMVGYPHDVEEVYFGIDGILENANEGTITIDFTTSTPTLAKRINEAGKSKNIYR
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]