Click here to switch to the map view.

The map label for this gene is metE [H]

Identifier: 227816779

GI number: 227816779

Start: 3860955

End: 3863243

Strand: Direct

Name: metE [H]

Synonym: BAMEG_4259

Alternate gene names: 227816779

Gene position: 3860955-3863243 (Clockwise)

Preceding gene: 227816778

Following gene: 227816782

Centisome position: 73.82

GC content: 36.3

Gene sequence:

>2289_bases
ATGGCAATTCAAACAAGTAACTTAGGTTATCCACGTATCGGATTACAACGAGAGTGGAAAAAAACATTGGAAGCTTTTTG
GTCCAATAAAATCAATGAAGAACAATTTTTAACAACAATGAAAGAAATTCGCCTTCAACACGTAAAAGTACAGCAAGAAA
AAGGGATTGAACTCATTCCAATTGGCGACTTTACATATTACGATCACGTTTTGGATACTGCTTATATGCTAGGATTTATC
CCATCACGTTTTTCTGAGTTTACATCTTACCTAGATGTATATTTTGCAATGGCGCGTGGCTCTAAAGATCACGTAGCTTC
CGAAATGACAAAATGGTTTAACACAAACTATCATTATATCGTTCCTGAATATGAAGAGGGATTACAAATCTCTTTAAAAG
ATAATCGTCCACTTCGCTTATACGAAGAGGCAAAACAAGAATTGGGTGTAGATGGAAAACCTGTTATTTTAGGACCATAT
ACTTTCTTGAAATTAGCTAAAGGCTATACACAAGAGCAATTTGCTACTATTTTAAAACAGTTAGTTGCACCTTACGTACA
ACTGCTTTCAGAACTACATGCAGCTGGTGCACAAATCATTCAAGTTGATGAACCGATTTTCGCTTCTTTAACGAAAGAAG
AAGTTCAACAAGCAAAAGAAATTTATGAAGCTATTCGTAAAGAGGTTCCAAATGCGACTCTTCTTTTACAAACATACTTT
GATAGTGTAGAAGAAAACTATGAAGAAATTATTACATTCCCAGTATCAAGTATTGGATTAGATTTCGTTCATGGTAAAGA
AGGTAATTTAAATGCTGTTTCAAAATATGGATTCCCAGCTGATAAAACTTTAGCTGTTGGTTGTATAGATGGCCGTAACA
TTTGGAGAGCTGACCTTGATGAAGTTCTTACGTTATTTACAACGTTACAAAAACAAGTCCAAACGAAAGATCTCATCGTT
CAACCTTCTTGTAGCTTATTGCATACACCAATCGATAAAACAGAAGAAACTCACTTATCAACTGAGCTATTTGATGCGTT
GGCATTTGCAAATCAAAAATTAGAAGAGTTAGTTCTTATTCATTCCGCTCTGACTCAAGGTACAGAAAGCATTAGTAATG
AACTGGAAACATATCGAAACGTACATCATACAATTCGTTCATCTGCTGCACGTAACCGAGAAGATGTCAAAGCAGCACGA
ACAGCACTAAAAGAAGAAGATTTTTCACGTCCTCTTCCATTTGAAAAACGATACGAATTACAACAAGTTGCCCTAAAGTT
ACCGTTGTTACCAACAACGACTATCGGTAGCTTCCCTCAAACAACTGAAGTTCGCCAAACGCGAAAAGAATGGCGTAATG
GTATTATTTCAAATGAACAATATGAACAATTTATTGAAAAAGAGACAGAAAAATGGATTCGTTACCAAGAAGAAATTGGT
CTTGATGTTCTTGTTCATGGCGAGTTTGAAAGAACTGACATGGTCGAATATTTTGGTGAGCGCCTTGCTGGCTTCTCATT
CACTAAAAACGGTTGGGTACAATCATACGGTTCTCGTTGCGTAAAACCACCTGTTATTTATGGTGATGTAGCCTTTATTA
ACGGCATGACTATTAAGGAAACGGTTTATGCACAAAGCTTAACAGAGAAAGTTGTAAAAGGAATGTTAACTGGACCTGTT
ACGATTTTAAATTGGTCCTTCGTTCGAAATGACATTCCAAGAAAAGAAGTTTCGTATCAAATTGCATTAGCTCTTCGTCA
TGAAATTGAACTACTTGAATCTTCTGGAATTCGAGTGATCCAAGTCGATGAGCCAGCACTTCGTGAAGGAATGCCACTGA
AAGAAAAAGATTGGGACGCTTATATTACATGGGCAGTACAATCCTTCCTTTTAGCAACTTCTTCTGTAGCAAATGAAACA
CAAATTCATACGCATATGTGTTACAGTAACTTCGAAGATATTGTTGACGCGATTCGCGCATTAGATGCAGATGTGATTTC
TATCGAAACATCAAGAAGTCACGGAGAATTTATTGATACATTAAAACATACAACATACGAAAAGGGCATCGGTCTAGGTG
TATATGATATTCATAGCCCACGTGTACCAAGTAAAGATGAAATGTATAAAATCGTAGAACAATCTTTACAAGTATGCGAT
CCTAAATATTTCTGGATTAATCCTGATTGTGGTTTAAAAACGCGAAGAACAGAAGAAGTTATTCCAGCTCTAGAACATAT
GGTGCAAGCAGCAAAAGATGCTCGTTCCCTACTAAAAACAAACGCATAA

Upstream 100 bases:

>100_bases
AAACAAAAAGGAAGGCTTATTTTCTATGCCTTTATGCTTTTTGTTATAGGTATTTCCTAGTAAACGGAAGTGCTTACTTT
TAATTAGGGAGGAATTTGAA

Downstream 100 bases:

>100_bases
TAAAAATGGGTTATCCTCCAAAAGGATAACCCATTTTTATTCTTTACTTTCAAAAAAGAATTCGTATTGTATTTTATATA
ATTCATCATCCGTTGGTTCT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 762; Mature: 761

Protein sequence:

>762_residues
MAIQTSNLGYPRIGLQREWKKTLEAFWSNKINEEQFLTTMKEIRLQHVKVQQEKGIELIPIGDFTYYDHVLDTAYMLGFI
PSRFSEFTSYLDVYFAMARGSKDHVASEMTKWFNTNYHYIVPEYEEGLQISLKDNRPLRLYEEAKQELGVDGKPVILGPY
TFLKLAKGYTQEQFATILKQLVAPYVQLLSELHAAGAQIIQVDEPIFASLTKEEVQQAKEIYEAIRKEVPNATLLLQTYF
DSVEENYEEIITFPVSSIGLDFVHGKEGNLNAVSKYGFPADKTLAVGCIDGRNIWRADLDEVLTLFTTLQKQVQTKDLIV
QPSCSLLHTPIDKTEETHLSTELFDALAFANQKLEELVLIHSALTQGTESISNELETYRNVHHTIRSSAARNREDVKAAR
TALKEEDFSRPLPFEKRYELQQVALKLPLLPTTTIGSFPQTTEVRQTRKEWRNGIISNEQYEQFIEKETEKWIRYQEEIG
LDVLVHGEFERTDMVEYFGERLAGFSFTKNGWVQSYGSRCVKPPVIYGDVAFINGMTIKETVYAQSLTEKVVKGMLTGPV
TILNWSFVRNDIPRKEVSYQIALALRHEIELLESSGIRVIQVDEPALREGMPLKEKDWDAYITWAVQSFLLATSSVANET
QIHTHMCYSNFEDIVDAIRALDADVISIETSRSHGEFIDTLKHTTYEKGIGLGVYDIHSPRVPSKDEMYKIVEQSLQVCD
PKYFWINPDCGLKTRRTEEVIPALEHMVQAAKDARSLLKTNA

Sequences:

>Translated_762_residues
MAIQTSNLGYPRIGLQREWKKTLEAFWSNKINEEQFLTTMKEIRLQHVKVQQEKGIELIPIGDFTYYDHVLDTAYMLGFI
PSRFSEFTSYLDVYFAMARGSKDHVASEMTKWFNTNYHYIVPEYEEGLQISLKDNRPLRLYEEAKQELGVDGKPVILGPY
TFLKLAKGYTQEQFATILKQLVAPYVQLLSELHAAGAQIIQVDEPIFASLTKEEVQQAKEIYEAIRKEVPNATLLLQTYF
DSVEENYEEIITFPVSSIGLDFVHGKEGNLNAVSKYGFPADKTLAVGCIDGRNIWRADLDEVLTLFTTLQKQVQTKDLIV
QPSCSLLHTPIDKTEETHLSTELFDALAFANQKLEELVLIHSALTQGTESISNELETYRNVHHTIRSSAARNREDVKAAR
TALKEEDFSRPLPFEKRYELQQVALKLPLLPTTTIGSFPQTTEVRQTRKEWRNGIISNEQYEQFIEKETEKWIRYQEEIG
LDVLVHGEFERTDMVEYFGERLAGFSFTKNGWVQSYGSRCVKPPVIYGDVAFINGMTIKETVYAQSLTEKVVKGMLTGPV
TILNWSFVRNDIPRKEVSYQIALALRHEIELLESSGIRVIQVDEPALREGMPLKEKDWDAYITWAVQSFLLATSSVANET
QIHTHMCYSNFEDIVDAIRALDADVISIETSRSHGEFIDTLKHTTYEKGIGLGVYDIHSPRVPSKDEMYKIVEQSLQVCD
PKYFWINPDCGLKTRRTEEVIPALEHMVQAAKDARSLLKTNA
>Mature_761_residues
AIQTSNLGYPRIGLQREWKKTLEAFWSNKINEEQFLTTMKEIRLQHVKVQQEKGIELIPIGDFTYYDHVLDTAYMLGFIP
SRFSEFTSYLDVYFAMARGSKDHVASEMTKWFNTNYHYIVPEYEEGLQISLKDNRPLRLYEEAKQELGVDGKPVILGPYT
FLKLAKGYTQEQFATILKQLVAPYVQLLSELHAAGAQIIQVDEPIFASLTKEEVQQAKEIYEAIRKEVPNATLLLQTYFD
SVEENYEEIITFPVSSIGLDFVHGKEGNLNAVSKYGFPADKTLAVGCIDGRNIWRADLDEVLTLFTTLQKQVQTKDLIVQ
PSCSLLHTPIDKTEETHLSTELFDALAFANQKLEELVLIHSALTQGTESISNELETYRNVHHTIRSSAARNREDVKAART
ALKEEDFSRPLPFEKRYELQQVALKLPLLPTTTIGSFPQTTEVRQTRKEWRNGIISNEQYEQFIEKETEKWIRYQEEIGL
DVLVHGEFERTDMVEYFGERLAGFSFTKNGWVQSYGSRCVKPPVIYGDVAFINGMTIKETVYAQSLTEKVVKGMLTGPVT
ILNWSFVRNDIPRKEVSYQIALALRHEIELLESSGIRVIQVDEPALREGMPLKEKDWDAYITWAVQSFLLATSSVANETQ
IHTHMCYSNFEDIVDAIRALDADVISIETSRSHGEFIDTLKHTTYEKGIGLGVYDIHSPRVPSKDEMYKIVEQSLQVCDP
KYFWINPDCGLKTRRTEEVIPALEHMVQAAKDARSLLKTNA

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=764, Percent_Identity=45.5497382198953, Blast_Score=653, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=767, Percent_Identity=44.4589308996089, Blast_Score=638, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 87219; Mature: 87088

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIQTSNLGYPRIGLQREWKKTLEAFWSNKINEEQFLTTMKEIRLQHVKVQQEKGIELIP
CCEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IGDFTYYDHVLDTAYMLGFIPSRFSEFTSYLDVYFAMARGSKDHVASEMTKWFNTNYHYI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEE
VPEYEEGLQISLKDNRPLRLYEEAKQELGVDGKPVILGPYTFLKLAKGYTQEQFATILKQ
CCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCEEECCHHHHHHHCCCCHHHHHHHHHH
LVAPYVQLLSELHAAGAQIIQVDEPIFASLTKEEVQQAKEIYEAIRKEVPNATLLLQTYF
HHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHH
DSVEENYEEIITFPVSSIGLDFVHGKEGNLNAVSKYGFPADKTLAVGCIDGRNIWRADLD
HHHHHHHHHHHCCCHHHHCCEEEECCCCCCCHHHHCCCCCCCEEEEEEECCCCCCHHHHH
EVLTLFTTLQKQVQTKDLIVQPSCSLLHTPIDKTEETHLSTELFDALAFANQKLEELVLI
HHHHHHHHHHHHHHHHHEEECCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HSALTQGTESISNELETYRNVHHTIRSSAARNREDVKAARTALKEEDFSRPLPFEKRYEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHH
QQVALKLPLLPTTTIGSFPQTTEVRQTRKEWRNGIISNEQYEQFIEKETEKWIRYQEEIG
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LDVLVHGEFERTDMVEYFGERLAGFSFTKNGWVQSYGSRCVKPPVIYGDVAFINGMTIKE
CEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCEEECCHHHHCCCCHHH
TVYAQSLTEKVVKGMLTGPVTILNWSFVRNDIPRKEVSYQIALALRHEIELLESSGIRVI
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
QVDEPALREGMPLKEKDWDAYITWAVQSFLLATSSVANETQIHTHMCYSNFEDIVDAIRA
EECCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LDADVISIETSRSHGEFIDTLKHTTYEKGIGLGVYDIHSPRVPSKDEMYKIVEQSLQVCD
CCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCC
PKYFWINPDCGLKTRRTEEVIPALEHMVQAAKDARSLLKTNA
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIQTSNLGYPRIGLQREWKKTLEAFWSNKINEEQFLTTMKEIRLQHVKVQQEKGIELIP
CEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IGDFTYYDHVLDTAYMLGFIPSRFSEFTSYLDVYFAMARGSKDHVASEMTKWFNTNYHYI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEE
VPEYEEGLQISLKDNRPLRLYEEAKQELGVDGKPVILGPYTFLKLAKGYTQEQFATILKQ
CCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCEEECCHHHHHHHCCCCHHHHHHHHHH
LVAPYVQLLSELHAAGAQIIQVDEPIFASLTKEEVQQAKEIYEAIRKEVPNATLLLQTYF
HHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHH
DSVEENYEEIITFPVSSIGLDFVHGKEGNLNAVSKYGFPADKTLAVGCIDGRNIWRADLD
HHHHHHHHHHHCCCHHHHCCEEEECCCCCCCHHHHCCCCCCCEEEEEEECCCCCCHHHHH
EVLTLFTTLQKQVQTKDLIVQPSCSLLHTPIDKTEETHLSTELFDALAFANQKLEELVLI
HHHHHHHHHHHHHHHHHEEECCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HSALTQGTESISNELETYRNVHHTIRSSAARNREDVKAARTALKEEDFSRPLPFEKRYEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHH
QQVALKLPLLPTTTIGSFPQTTEVRQTRKEWRNGIISNEQYEQFIEKETEKWIRYQEEIG
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LDVLVHGEFERTDMVEYFGERLAGFSFTKNGWVQSYGSRCVKPPVIYGDVAFINGMTIKE
CEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCEEECCHHHHCCCCHHH
TVYAQSLTEKVVKGMLTGPVTILNWSFVRNDIPRKEVSYQIALALRHEIELLESSGIRVI
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
QVDEPALREGMPLKEKDWDAYITWAVQSFLLATSSVANETQIHTHMCYSNFEDIVDAIRA
EECCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LDADVISIETSRSHGEFIDTLKHTTYEKGIGLGVYDIHSPRVPSKDEMYKIVEQSLQVCD
CCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCC
PKYFWINPDCGLKTRRTEEVIPALEHMVQAAKDARSLLKTNA
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA