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The map label for this gene is malK [H]

Identifier: 227816553

GI number: 227816553

Start: 3652958

End: 3654517

Strand: Reverse

Name: malK [H]

Synonym: BAMEG_4010

Alternate gene names: 227816553

Gene position: 3654517-3652958 (Counterclockwise)

Preceding gene: 227816554

Following gene: 227816552

Centisome position: 69.87

GC content: 33.85

Gene sequence:

>1560_bases
TTGTTAGTTTGTACAGTTGTAATTTTTTCTTTACTTGTGACAGATATATTAATTAGTCACAATGTAGAACGGACAACGGA
GGATAGCCAAGCAGAGAAAGCAAAAACAATTGCACACATTGTGGCGAATGATTCAATTGTAATCGATGGTTTAATTGGGA
AAGCAGATACTTCTGCAATTCAAACGTATACAAATAGAATATTAAAAAACACAGGTGTTCAATTTATTGTAGTTATGGAC
ATGAATGGAATAAGAAAGTCACATCCAAATCCTCAAAAAATAGGTCATCATTTTATTGGGGGAGATGAAGGACCTGCATT
GAAAGGAAAGGAACATGTATCGCTGGCAGAAGGAACTTTAGGTATTTCCATGCGAGTGTTTGTACCAATATTTTCTGAAA
CAGGTGAACAACTTGGAGCAGTGGCTGTTGGTATTTCAGCAGATAATGTAAAAGAGAGAGTTAAGGAAAGTAGACATATC
ATTTATATTGGTGTCGGAGTTGGTGTATTAGTCGGAATTATAGGAGCAATATTGCTAGCTAGACATATAAAGAAAAGTTT
ATTCGGTCTTGAGCCGCACAGGATAGCAAAAATTCTTGAAGAAAGAAATACGATGCTACAATCTGTAAAGGAAGGTATTA
TTGCTGTAGATAAAGAGGCTAGAGTAACGTTAATTAATAATGAAGCGAAACGTCTATTTAAAAAAAGCGGACTTGAAGAA
GATTTTATCGGTAAAGATGTTGAGTTGTATATGCCAAATTCACGTATAAAAGAAGTATTGCAAACGGGAGAAGTACAATT
AAACGAAGAACAAAATATTTACGGAATTACGATTGTGACGAATCGAGTTCCTTTATATGTAAAAGAAGAAATAGTTGGTG
CAATTGCAACATTTCGTGATAAAACAGAGATTAGAAAACTAGCAGAGGAACTAACTGGTATTAGACTATATGCAGAAGCA
TTACGGGCACAATCTCATGAGTTTATGAATAAGATGCATGTCGTATTAGGGCTTACACATATGAAACAGTATGAAGAATT
ACAGAAGTATATAAGCGGCATGGTATCAGAGCATCAATATGAAATTGGTGGAGTTATGAAAAGAATAAAAAGTCCAGTAT
TTGCTGGTTTTTTACTAGGTAAGCTCAGCTATGCTAGAGAGAAAAATATAAAATTAATTATAAGTGAAGATTCTTATATG
CCAGAAATAGATGATGAAAGTATTACTCATGAACTTATTACGATTGTCGGAAATTTAATTGATAATGCATTAGAGGCAGT
TACGAATTGTGAAAAGAAGCGAGTTGAAGTTAAGATACAACATGAGGATATACTAACTATTACAGTACAAGATACGGGAA
AAGGTATACAAGAAAAAGAAATAGAGGAATTATTTACGAAAGGTTATTCCACAAAGGGAGATAACCGCGGTTATGGTTTG
TATCTTGTAAAAGAAAGTATACAGCGAATAAATGGGGAAATTCATATGCATTCATTAGTAGGTAAGGGAACAACGATAAC
AATTGAAATACCTAAAGGTAGGGATGAGAGGCAAATATGA

Upstream 100 bases:

>100_bases
ATAATATCACCTTACTTATTTTTCTATTCAGCAATTAGGGGAGTCATTAACGGGATGAAAAAAAAGGAAAAGACTATGGA
ATTTATGGAAAACGATTACA

Downstream 100 bases:

>100_bases
TTAAAGTTTTAATTGTAGAAGATGACCCGATGGTAGCAATGCTAAATACGCATTATTTAGAGCAAGTAGGAGGATTTGAA
CTTGTTCAAGCAGTTAATTC

Product: sensory histidine kinase DcuS

Products: NA

Alternate protein names: Malate kinase sensor [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDSIVIDGLIGKADTSAIQTYTNRILKNTGVQFIVVMD
MNGIRKSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTLGISMRVFVPIFSETGEQLGAVAVGISADNVKERVKESRHI
IYIGVGVGVLVGIIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEARVTLINNEAKRLFKKSGLEE
DFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPLYVKEEIVGAIATFRDKTEIRKLAEELTGIRLYAEA
LRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGVMKRIKSPVFAGFLLGKLSYAREKNIKLIISEDSYM
PEIDDESITHELITIVGNLIDNALEAVTNCEKKRVEVKIQHEDILTITVQDTGKGIQEKEIEELFTKGYSTKGDNRGYGL
YLVKESIQRINGEIHMHSLVGKGTTITIEIPKGRDERQI

Sequences:

>Translated_519_residues
MLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDSIVIDGLIGKADTSAIQTYTNRILKNTGVQFIVVMD
MNGIRKSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTLGISMRVFVPIFSETGEQLGAVAVGISADNVKERVKESRHI
IYIGVGVGVLVGIIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEARVTLINNEAKRLFKKSGLEE
DFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPLYVKEEIVGAIATFRDKTEIRKLAEELTGIRLYAEA
LRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGVMKRIKSPVFAGFLLGKLSYAREKNIKLIISEDSYM
PEIDDESITHELITIVGNLIDNALEAVTNCEKKRVEVKIQHEDILTITVQDTGKGIQEKEIEELFTKGYSTKGDNRGYGL
YLVKESIQRINGEIHMHSLVGKGTTITIEIPKGRDERQI
>Mature_519_residues
MLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDSIVIDGLIGKADTSAIQTYTNRILKNTGVQFIVVMD
MNGIRKSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTLGISMRVFVPIFSETGEQLGAVAVGISADNVKERVKESRHI
IYIGVGVGVLVGIIGAILLARHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEARVTLINNEAKRLFKKSGLEE
DFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPLYVKEEIVGAIATFRDKTEIRKLAEELTGIRLYAEA
LRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGVMKRIKSPVFAGFLLGKLSYAREKNIKLIISEDSYM
PEIDDESITHELITIVGNLIDNALEAVTNCEKKRVEVKIQHEDILTITVQDTGKGIQEKEIEELFTKGYSTKGDNRGYGL
YLVKESIQRINGEIHMHSLVGKGTTITIEIPKGRDERQI

Specific function: Member of a two-component regulatory system malK/malR. Involved in the activation of maeA, maeN and yflS in presence of malate. Probably activates malR by phosphorylation [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=515, Percent_Identity=40.3883495145631, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1786837, Length=526, Percent_Identity=30.6083650190114, Blast_Score=244, Evalue=7e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57883; Mature: 57883

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDSIVIDGLIGKADTSAI
CEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
QTYTNRILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTL
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCEEECCCC
GISMRVFVPIFSETGEQLGAVAVGISADNVKERVKESRHIIYIGVGVGVLVGIIGAILLA
CEEEEEEEEEHHHCCHHHCEEEEECCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHH
RHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEARVTLINNEAKRLFKKSGLEE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEECHHHHHHHHHCCCCH
DFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPLYVKEEIVGAIATFRD
HHCCCCEEEECCCHHHHHHHHCCCEEECCCCCEEEEEEEECCCCEEEHHHHHHHHHHHCC
KTEIRKLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGGVMKRIKSPVFAGFLLGKLSYAREKNIKLIISEDSYMPEIDDESITHELITIVGNLI
HHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
DNALEAVTNCEKKRVEVKIQHEDILTITVQDTGKGIQEKEIEELFTKGYSTKGDNRGYGL
HHHHHHHHCCCCCEEEEEEEECCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCEEE
YLVKESIQRINGEIHMHSLVGKGTTITIEIPKGRDERQI
EEEHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDSIVIDGLIGKADTSAI
CEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
QTYTNRILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTL
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCEEECCCC
GISMRVFVPIFSETGEQLGAVAVGISADNVKERVKESRHIIYIGVGVGVLVGIIGAILLA
CEEEEEEEEEHHHCCHHHCEEEEECCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHH
RHIKKSLFGLEPHRIAKILEERNTMLQSVKEGIIAVDKEARVTLINNEAKRLFKKSGLEE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEECHHHHHHHHHCCCCH
DFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPLYVKEEIVGAIATFRD
HHCCCCEEEECCCHHHHHHHHCCCEEECCCCCEEEEEEEECCCCEEEHHHHHHHHHHHCC
KTEIRKLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGGVMKRIKSPVFAGFLLGKLSYAREKNIKLIISEDSYMPEIDDESITHELITIVGNLI
HHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
DNALEAVTNCEKKRVEVKIQHEDILTITVQDTGKGIQEKEIEELFTKGYSTKGDNRGYGL
HHHHHHHHCCCCCEEEEEEEECCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCEEE
YLVKESIQRINGEIHMHSLVGKGTTITIEIPKGRDERQI
EEEHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 12949159; 12949160 [H]