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The map label for this gene is ytwF [H]

Identifier: 227816349

GI number: 227816349

Start: 3442243

End: 3442539

Strand: Reverse

Name: ytwF [H]

Synonym: BAMEG_3778

Alternate gene names: 227816349

Gene position: 3442539-3442243 (Counterclockwise)

Preceding gene: 227816350

Following gene: 227816348

Centisome position: 65.82

GC content: 37.04

Gene sequence:

>297_bases
ATGAAAGAAATGATAGCAAAAGAATTAGAAGAAAAATTGTTACGTAAAGAAGCAGTAAATATCGTAGACGTACGTGAAGT
AGAAGAAGTAGCTGAAGGGAAAATTCCAGAAGCGTGTAACATTCCACTAGGGCTGTTAGAATTTCGTATGCATGAGTTGG
ATAAAAAGAAAGAGTATATTATCGTTTGTCGTTCTGGCGGAAGAAGTGCAAGGGCAGTTCAATTTTTAGAAGGTTACGGT
TTTCGAGTGATTAATATGGTAGGTGGTATGTTAGCGTGGGAAGGTAAAGTAGAATAG

Upstream 100 bases:

>100_bases
TGAGAAGTAAACAAGCAGCGAAAATGTTAAAAAAATTAGGATTTCAGCATGTTATCAATGTTTCAGGCGGGATGAATGGT
TTGTGAGGAGGAAATGTAAA

Downstream 100 bases:

>100_bases
GGCGAGACGATTTTTTATAAAAATAAAAATACCCCTATAGGTAATTGGAGGGAGAACAACATGGAACAACAGAAGAAAAC
AACAATCGTTTTATTTAGCG

Product: rhodanese domain family protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 98; Mature: 98

Protein sequence:

>98_residues
MKEMIAKELEEKLLRKEAVNIVDVREVEEVAEGKIPEACNIPLGLLEFRMHELDKKKEYIIVCRSGGRSARAVQFLEGYG
FRVINMVGGMLAWEGKVE

Sequences:

>Translated_98_residues
MKEMIAKELEEKLLRKEAVNIVDVREVEEVAEGKIPEACNIPLGLLEFRMHELDKKKEYIIVCRSGGRSARAVQFLEGYG
FRVINMVGGMLAWEGKVE
>Mature_98_residues
MKEMIAKELEEKLLRKEAVNIVDVREVEEVAEGKIPEACNIPLGLLEFRMHELDKKKEYIIVCRSGGRSARAVQFLEGYG
FRVINMVGGMLAWEGKVE

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 11166; Mature: 11166

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEMIAKELEEKLLRKEAVNIVDVREVEEVAEGKIPEACNIPLGLLEFRMHELDKKKEYI
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEE
IVCRSGGRSARAVQFLEGYGFRVINMVGGMLAWEGKVE
EEECCCCCCHHHHHHHHCCCCHHHHHHCCHHCCCCCCC
>Mature Secondary Structure
MKEMIAKELEEKLLRKEAVNIVDVREVEEVAEGKIPEACNIPLGLLEFRMHELDKKKEYI
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEE
IVCRSGGRSARAVQFLEGYGFRVINMVGGMLAWEGKVE
EEECCCCCCHHHHHHHHCCCCHHHHHHCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]