Click here to switch to the map view.

The map label for this gene is ybbF [H]

Identifier: 227816305

GI number: 227816305

Start: 3402640

End: 3404004

Strand: Reverse

Name: ybbF [H]

Synonym: BAMEG_3733

Alternate gene names: 227816305

Gene position: 3404004-3402640 (Counterclockwise)

Preceding gene: 227816306

Following gene: 227816304

Centisome position: 65.08

GC content: 40.07

Gene sequence:

>1365_bases
ATGAAGAAAGAAGAGAGAATGGCGAAAGAAATTTCAGAGCAACTTGGGGGAGTAAAAAATATTCGTGGCATTGCTCATTG
TATGACGAGATTACGATTAACGCTTCATGATGAAAGTAAAGTAAACATGGACCTTTTGAAAAAGGTAGAAGGGGTTATGG
GCGTTATTGAAGATGAGACGCTTCAAGTTGTTGTTGGTCCAGGAACGGTAAATAAAGTAGCAGCTGAGATGGAAGGTTTA
ACGGGACTCCGAATTGGTGAGGTGGCAGATTATCACCTTGAAGATCTTGGGCAAGAGATGAAGTCAGAGATTAAGAAGAA
AAATAATACACCAGTGAAAAACTTTTTAAGAAAAATAGGAAGTATCTTTATTCCATTAATTCCGGGTCTTGTTGCATCAG
GTATTATAAACGGGGTTGCTAACTTTGCGAAAAACGCAGGTGCTGATCCGAATGCAACTTGGCTACAAATGTTACTACTT
ATTGGTGGCGGTATCTTTACATTCTTAGGCATTTTAGTCGGCTGGAATACAGCGAAAGAGTTTGGCGGGACGCCAGTTCT
TGGGGCAATTGCTGGTATTTTAATTTTCAACCCGGCAATGGCGAATGTAAAATTATTTGGTGAAGCGCTCGTACCCGGAC
GGGGCGGATTATTTGCAGTTATTTTTGCAGCATGGCTTATGGTTGTAGTGGAAAGACAAGTTCGAAAAGCTGTACCAAAT
GCAGTTGATATTATTATAACGCCGCTTATTACTGTGTTAGTCGTAAGTATCGTAACGATGTTAGCAATTCAGCCGGTAGC
AGGTTTCTTATCTGAAGGAATTACAAACGGAATTAATGGCATTTTAAATATTGGCGGAGCGTTTGCGGGAGCAGTACTTG
CAGGAACATTTTTACCGCTCGTTATGGTCGGATTACATCACGGTTTAACACCGATTCATATGGAGTTTATTAATCAAACG
CACGTAACGCCTTTATTACCAGTACTCGCAATGGCTGGAGCTGGTCAAGTAGGTGCAGCGATTGCTATTTTTGTAAAAAC
AAAAAACAAACGACTTCGTAACGTAATTAAAGGGGCATTACCAGTTGGATTTTTAGGGATTGGTGAACCGTTATTATACG
GGGTTACATTACCACTGGGAAAACCGTTTCTTACAGCTTGTTTAGGAGCAGCAGTTGGCGGTGCGTTCCAAGCAGTTATG
AAAACAGCTGCACTCGGAATCGGTGTATCTGGATTATCATTAATTCCGTTAATTGCTGATAATAAATATTTATTATATTT
CCTTGGATTAGTAGTGGCATATACGTTCGGATTCATCTTTACGTACTTCTTCGGATTTAAAGAAGAAATGGCAGAAAACA
TATAG

Upstream 100 bases:

>100_bases
GCTATTGCAGTGTGAGTATGGGGAAGCACTGGAGAAACTAAAACAAGCAAAAGGGTTTGTGAAGAAGGCATTATAAAATA
ATAGGGAGGGGGATTCTATT

Downstream 100 bases:

>100_bases
AGAAAGGGATTCCTTTCTCTTTTTTTATTCATAGAAAGGGAGATTCATATGCTAGGAGTATCAATTTATCTTTCAAAAGA
GCGAGTAAAGAAACAAGAAG

Product: PTS system, sucrose-specific IIBC component

Products: NA

Alternate protein names: Phosphotransferase enzyme IIB component; PTS system EIIB component; Permease IIC component; PTS system EIIC component [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MKKEERMAKEISEQLGGVKNIRGIAHCMTRLRLTLHDESKVNMDLLKKVEGVMGVIEDETLQVVVGPGTVNKVAAEMEGL
TGLRIGEVADYHLEDLGQEMKSEIKKKNNTPVKNFLRKIGSIFIPLIPGLVASGIINGVANFAKNAGADPNATWLQMLLL
IGGGIFTFLGILVGWNTAKEFGGTPVLGAIAGILIFNPAMANVKLFGEALVPGRGGLFAVIFAAWLMVVVERQVRKAVPN
AVDIIITPLITVLVVSIVTMLAIQPVAGFLSEGITNGINGILNIGGAFAGAVLAGTFLPLVMVGLHHGLTPIHMEFINQT
HVTPLLPVLAMAGAGQVGAAIAIFVKTKNKRLRNVIKGALPVGFLGIGEPLLYGVTLPLGKPFLTACLGAAVGGAFQAVM
KTAALGIGVSGLSLIPLIADNKYLLYFLGLVVAYTFGFIFTYFFGFKEEMAENI

Sequences:

>Translated_454_residues
MKKEERMAKEISEQLGGVKNIRGIAHCMTRLRLTLHDESKVNMDLLKKVEGVMGVIEDETLQVVVGPGTVNKVAAEMEGL
TGLRIGEVADYHLEDLGQEMKSEIKKKNNTPVKNFLRKIGSIFIPLIPGLVASGIINGVANFAKNAGADPNATWLQMLLL
IGGGIFTFLGILVGWNTAKEFGGTPVLGAIAGILIFNPAMANVKLFGEALVPGRGGLFAVIFAAWLMVVVERQVRKAVPN
AVDIIITPLITVLVVSIVTMLAIQPVAGFLSEGITNGINGILNIGGAFAGAVLAGTFLPLVMVGLHHGLTPIHMEFINQT
HVTPLLPVLAMAGAGQVGAAIAIFVKTKNKRLRNVIKGALPVGFLGIGEPLLYGVTLPLGKPFLTACLGAAVGGAFQAVM
KTAALGIGVSGLSLIPLIADNKYLLYFLGLVVAYTFGFIFTYFFGFKEEMAENI
>Mature_454_residues
MKKEERMAKEISEQLGGVKNIRGIAHCMTRLRLTLHDESKVNMDLLKKVEGVMGVIEDETLQVVVGPGTVNKVAAEMEGL
TGLRIGEVADYHLEDLGQEMKSEIKKKNNTPVKNFLRKIGSIFIPLIPGLVASGIINGVANFAKNAGADPNATWLQMLLL
IGGGIFTFLGILVGWNTAKEFGGTPVLGAIAGILIFNPAMANVKLFGEALVPGRGGLFAVIFAAWLMVVVERQVRKAVPN
AVDIIITPLITVLVVSIVTMLAIQPVAGFLSEGITNGINGILNIGGAFAGAVLAGTFLPLVMVGLHHGLTPIHMEFINQT
HVTPLLPVLAMAGAGQVGAAIAIFVKTKNKRLRNVIKGALPVGFLGIGEPLLYGVTLPLGKPFLTACLGAAVGGAFQAVM
KTAALGIGVSGLSLIPLIADNKYLLYFLGLVVAYTFGFIFTYFFGFKEEMAENI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788769, Length=480, Percent_Identity=39.375, Blast_Score=271, Evalue=8e-74,
Organism=Escherichia coli, GI1790159, Length=424, Percent_Identity=30.4245283018868, Blast_Score=178, Evalue=8e-46,
Organism=Escherichia coli, GI2367362, Length=442, Percent_Identity=25.3393665158371, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI48994906, Length=478, Percent_Identity=27.8242677824268, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 48033; Mature: 48033

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEERMAKEISEQLGGVKNIRGIAHCMTRLRLTLHDESKVNMDLLKKVEGVMGVIEDET
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCC
LQVVVGPGTVNKVAAEMEGLTGLRIGEVADYHLEDLGQEMKSEIKKKNNTPVKNFLRKIG
EEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SIFIPLIPGLVASGIINGVANFAKNAGADPNATWLQMLLLIGGGIFTFLGILVGWNTAKE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCHHHH
FGGTPVLGAIAGILIFNPAMANVKLFGEALVPGRGGLFAVIFAAWLMVVVERQVRKAVPN
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCH
AVDIIITPLITVLVVSIVTMLAIQPVAGFLSEGITNGINGILNIGGAFAGAVLAGTFLPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMVGLHHGLTPIHMEFINQTHVTPLLPVLAMAGAGQVGAAIAIFVKTKNKRLRNVIKGAL
HHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHC
PVGFLGIGEPLLYGVTLPLGKPFLTACLGAAVGGAFQAVMKTAALGIGVSGLSLIPLIAD
CCCHHCCCCHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEC
NKYLLYFLGLVVAYTFGFIFTYFFGFKEEMAENI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKEERMAKEISEQLGGVKNIRGIAHCMTRLRLTLHDESKVNMDLLKKVEGVMGVIEDET
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCC
LQVVVGPGTVNKVAAEMEGLTGLRIGEVADYHLEDLGQEMKSEIKKKNNTPVKNFLRKIG
EEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SIFIPLIPGLVASGIINGVANFAKNAGADPNATWLQMLLLIGGGIFTFLGILVGWNTAKE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCHHHH
FGGTPVLGAIAGILIFNPAMANVKLFGEALVPGRGGLFAVIFAAWLMVVVERQVRKAVPN
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCH
AVDIIITPLITVLVVSIVTMLAIQPVAGFLSEGITNGINGILNIGGAFAGAVLAGTFLPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMVGLHHGLTPIHMEFINQTHVTPLLPVLAMAGAGQVGAAIAIFVKTKNKRLRNVIKGAL
HHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHC
PVGFLGIGEPLLYGVTLPLGKPFLTACLGAAVGGAFQAVMKTAALGIGVSGLSLIPLIAD
CCCHHCCCCHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEC
NKYLLYFLGLVVAYTFGFIFTYFFGFKEEMAENI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]