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The map label for this gene is cwlH [H]

Identifier: 227816260

GI number: 227816260

Start: 3357080

End: 3358126

Strand: Direct

Name: cwlH [H]

Synonym: BAMEG_3687

Alternate gene names: 227816260

Gene position: 3357080-3358126 (Clockwise)

Preceding gene: 227816259

Following gene: 227816263

Centisome position: 64.19

GC content: 37.54

Gene sequence:

>1047_bases
ATGATTGTTCCAAAAGGGAATGAAAATATTCGTCCTGGCTACGCAATGGAACCGAAATACATTACAATTCATGAAACTGC
TAATACAAGTAAAGGTGCGAACGCCTTAAATCATGCGAAGTACTTAGATAATCAAGCCCGCGGAAATACTGATCGTTCAG
CATCTTGGCATTTTACAGTAGATGACAAAGAAATTTATCAACATTTACCATTAAATGAAGTTGGTTGGCATGCCGGAAAT
AAAACTGGAAACTATGAATCTATCGGAATTGAAATTGCTGTGAATAGTGATGGGAATTATACGAAAGCGGTAGAAAACGC
AAAAAAGTTAGCTGCTTATTTAATGAATGAATTAAACATATCTCTAGATCACGTGCAAAAGCATCAATTTTGGAGCGGAA
AAAACTGCCCTGCATTTATGATTCAACGCGGACAATGGGATGCTTTCTTAAAAGGTACGAACGCTTACTATAATGAACAT
CATAAAGATGTTATTCCGCCGCCAGAAGTCCCTCATGAAAAAGACGACATTACAGGTGGATGGTATGAACAAGACATTCG
TCAATTGGCTGCTAGAAAAATCATGTTCGGTGACGGCAATGGTTCTTACTGGCCAAACCGTCTTGTAACAAGAGCTGAGT
TTGCAAATTTAATGTCGAGAGCTTTAAATTTACCTGCTGGAAACGCTAAATTTACAGATTTAAACGAAGCACATCCATCT
TTAGTAGATGGAATTAACCGCGCCGCTAGCGCAGGTATCATTAATGGTCGTGGGAATAATAAATTTGATCCTAATGCTAC
AATAACACGTGATGAAGCTGTTATTATGATTGACCGTGCACTAGAATATAACTGGATTTATAGAAAAGAAGTGAAATTAC
CATTCACTGACCAACACTTAGCATACGATAAAAAAGCATTACAAAACGTTTATGCATATGGAATTGTAAAAGGGAACGAA
CGAAATGAATTCGTACCAAAAGGCACAGCAACACGCGCTGAATCCGCCGCATTTTTAAATCGAATGCTTAAAGTTATTGA
AGCTTAA

Upstream 100 bases:

>100_bases
TATTCGAAAAAAAGTTTCTATGGAAAATAGAAGCGCCGCATTTTTCAAAACACCATCTCATTTACCTACAAATAACGTTT
ATGGTGTTACATTTAAAGAA

Downstream 100 bases:

>100_bases
ATAAAAAAACAAAAGGCATAAGAGATAATATTTCTCTTATGCCTTTTATAAAACATATTAGTTATTTTTAGTCATAAATC
TTAAGCGTATCCCTAATAAA

Product: N-acetylmuramoyl-L-alanine amidase, family 2

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MIVPKGNENIRPGYAMEPKYITIHETANTSKGANALNHAKYLDNQARGNTDRSASWHFTVDDKEIYQHLPLNEVGWHAGN
KTGNYESIGIEIAVNSDGNYTKAVENAKKLAAYLMNELNISLDHVQKHQFWSGKNCPAFMIQRGQWDAFLKGTNAYYNEH
HKDVIPPPEVPHEKDDITGGWYEQDIRQLAARKIMFGDGNGSYWPNRLVTRAEFANLMSRALNLPAGNAKFTDLNEAHPS
LVDGINRAASAGIINGRGNNKFDPNATITRDEAVIMIDRALEYNWIYRKEVKLPFTDQHLAYDKKALQNVYAYGIVKGNE
RNEFVPKGTATRAESAAFLNRMLKVIEA

Sequences:

>Translated_348_residues
MIVPKGNENIRPGYAMEPKYITIHETANTSKGANALNHAKYLDNQARGNTDRSASWHFTVDDKEIYQHLPLNEVGWHAGN
KTGNYESIGIEIAVNSDGNYTKAVENAKKLAAYLMNELNISLDHVQKHQFWSGKNCPAFMIQRGQWDAFLKGTNAYYNEH
HKDVIPPPEVPHEKDDITGGWYEQDIRQLAARKIMFGDGNGSYWPNRLVTRAEFANLMSRALNLPAGNAKFTDLNEAHPS
LVDGINRAASAGIINGRGNNKFDPNATITRDEAVIMIDRALEYNWIYRKEVKLPFTDQHLAYDKKALQNVYAYGIVKGNE
RNEFVPKGTATRAESAAFLNRMLKVIEA
>Mature_348_residues
MIVPKGNENIRPGYAMEPKYITIHETANTSKGANALNHAKYLDNQARGNTDRSASWHFTVDDKEIYQHLPLNEVGWHAGN
KTGNYESIGIEIAVNSDGNYTKAVENAKKLAAYLMNELNISLDHVQKHQFWSGKNCPAFMIQRGQWDAFLKGTNAYYNEH
HKDVIPPPEVPHEKDDITGGWYEQDIRQLAARKIMFGDGNGSYWPNRLVTRAEFANLMSRALNLPAGNAKFTDLNEAHPS
LVDGINRAASAGIINGRGNNKFDPNATITRDEAVIMIDRALEYNWIYRKEVKLPFTDQHLAYDKKALQNVYAYGIVKGNE
RNEFVPKGTATRAESAAFLNRMLKVIEA

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 39194; Mature: 39194

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVPKGNENIRPGYAMEPKYITIHETANTSKGANALNHAKYLDNQARGNTDRSASWHFTV
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEE
DDKEIYQHLPLNEVGWHAGNKTGNYESIGIEIAVNSDGNYTKAVENAKKLAAYLMNELNI
CHHHHHHHCCCCHHCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
SLDHVQKHQFWSGKNCPAFMIQRGQWDAFLKGTNAYYNEHHKDVIPPPEVPHEKDDITGG
CHHHHHHHHCCCCCCCCEEEEECCCCCEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCC
WYEQDIRQLAARKIMFGDGNGSYWPNRLVTRAEFANLMSRALNLPAGNAKFTDLNEAHPS
HHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
LVDGINRAASAGIINGRGNNKFDPNATITRDEAVIMIDRALEYNWIYRKEVKLPFTDQHL
HHHHHHHHHHCCEEECCCCCCCCCCCEEECCCEEEEEECCCCCCEEEEEEECCCCCCHHH
AYDKKALQNVYAYGIVKGNERNEFVPKGTATRAESAAFLNRMLKVIEA
HHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIVPKGNENIRPGYAMEPKYITIHETANTSKGANALNHAKYLDNQARGNTDRSASWHFTV
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEE
DDKEIYQHLPLNEVGWHAGNKTGNYESIGIEIAVNSDGNYTKAVENAKKLAAYLMNELNI
CHHHHHHHCCCCHHCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
SLDHVQKHQFWSGKNCPAFMIQRGQWDAFLKGTNAYYNEHHKDVIPPPEVPHEKDDITGG
CHHHHHHHHCCCCCCCCEEEEECCCCCEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCC
WYEQDIRQLAARKIMFGDGNGSYWPNRLVTRAEFANLMSRALNLPAGNAKFTDLNEAHPS
HHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
LVDGINRAASAGIINGRGNNKFDPNATITRDEAVIMIDRALEYNWIYRKEVKLPFTDQHL
HHHHHHHHHHCCEEECCCCCCCCCCCEEECCCEEEEEECCCCCCEEEEEEECCCCCCHHH
AYDKKALQNVYAYGIVKGNERNEFVPKGTATRAESAAFLNRMLKVIEA
HHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]