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The map label for this gene is leuA

Identifier: 227815752

GI number: 227815752

Start: 2894005

End: 2895525

Strand: Reverse

Name: leuA

Synonym: BAMEG_3175

Alternate gene names: 227815752

Gene position: 2895525-2894005 (Counterclockwise)

Preceding gene: 227815753

Following gene: 227815751

Centisome position: 55.36

GC content: 38.4

Gene sequence:

>1521_bases
ATGAAGCAAATTTTGTTCATGGATACGACGCTACGTGATGGCGAACAATCACCAGGAGTAAATTTAAATGAACAAGAGAA
ATTGCAAATTGCAAGGCAATTAGAGAGACTAGGGATTCATGTCATGGAAGCTGGCTTTGCAGCAGCTTCCGAAGGGGATT
TTCAATCGGTAAAGCGCATCGCAAATACAATTCAAAATGCTACAGTTATGAGTTTAGCAAGAGCGAAAGAAAGTGATATT
CGAAGAGCATATGAAGCTGTGAAAGGTGCGGTATCTCCTCGTTTACACGTATTTTTAGCTACGAGTGATATTCATATGAA
GTATAAACTTTGTATGTCAAAAGAAGATGTATTAGATAGTATTTATCGATCGGTTACACTTGGGAAATCGTTATTTCCGA
CAGTACAATTTTCAGCAGAAGATGCAACAAGAACAGCAAGAGATTTTTTAGCTGAAGCGGTAGAAGTTGCGATTCGTGCA
GGAGCGAATGTAATTAATATTCCAGATACAGTTGGGTATACGAATCCAGAAGAATATTATGCTCTTTTTAAATATTTACA
AGAATCCGTTCCTTCATATGAAAAAGCAATTTTCTCTTGTCATTGTCATGATGATCTTGGGATGGCGGTAGCAAATTCAT
TAGCTGCAGTTGAAGGCGGAGCGTTGCAAGTAGAAGGAACAATTAATGGGATTGGAGAAAGAGCAGGGAATGCGGCGTTA
GAAGAGGTTGCAGTTGCTCTTCATATTAGGAAAGATTTCTATAAAGCAGAGCCTTCTATGACGCTAAAAGAAATTAAAGC
GACAAGTACATTAGTAAGCAGATTAACGGGTATGGTTGTATCGAAAAATAAAGCGATTGTTGGAGCAAATGCATTCGCTC
ATGAATCAGGCATTCATCAAGATGGTGTATTAAAAGAAGTGACAACATATGAAATTATTGAACCAGCGCTAGTAGGCGAA
TCTCAAAATCTATTTGTACTTGGAAAACATTCTGGGCGTCACGCGTTTACAGAAAAAATGAAAGAACTTGGCTATGAATT
TACAAACGACGAGCGGGATGCAGTATTTGAAGCATTTAAAAAATTAGCTGATCGTAAGAAGGAAATTACTGAGGAAGATT
TACGAGCACTTATGCTTGGTGAAGCAGCATTTGCGGCTCAGCAATATAACATTACGCAATTGCAAGTACATTTCGTATCA
AATAGTACACAATGTGCGACAGTTGTACTAAAAGATGAGGAAGGAAATGTATTCGAAGATGCAGCTACTGGCTCTGGTAG
TATTGAAGCGATTTATAATGCAATCCAAAGAATTTTAGGATTGGAATGTGAATTGGCGGATTATCGCATACAATCTATTA
CACAAGGTCAAGATGCACTGGCTCATGTTCATGTTGAATTAAAAGAAGGAGCACATCAAGTATCAGGTTTTGGTGTTGCA
CAAGACGTATTGGAAGCGTCGGCAAGAGCATATGTCCACGCAGCGGGGAAATTGAAATCTTTTATCCAGCTTGTGAAATA
A

Upstream 100 bases:

>100_bases
AATGAAAAAGAGAATGAACATGAAATCGAAGTAGTTGGACGTAAATTACGTGGAATGATGCCTTTCGTACAGCCGCGAGT
AAAGGCAGGGGTCAAATAAT

Downstream 100 bases:

>100_bases
GTGTTTGATAGGTGAGAGGAGCTATTCTCGCCTATCAAATTAAAGTTCTGAAAATTCGGTTAAAAGGAGTGTTGAAATTG
GAAAAACGTATCGTTTGTTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRIANTIQNATVMSLARAKESDI
RRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDSIYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRA
GANVINIPDTVGYTNPEEYYALFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL
EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVSKNKAIVGANAFAHESGIHQDGVLKEVTTYEIIEPALVGE
SQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFKKLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVS
NSTQCATVVLKDEEGNVFEDAATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA
QDVLEASARAYVHAAGKLKSFIQLVK

Sequences:

>Translated_506_residues
MKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRIANTIQNATVMSLARAKESDI
RRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDSIYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRA
GANVINIPDTVGYTNPEEYYALFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL
EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVSKNKAIVGANAFAHESGIHQDGVLKEVTTYEIIEPALVGE
SQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFKKLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVS
NSTQCATVVLKDEEGNVFEDAATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA
QDVLEASARAYVHAAGKLKSFIQLVK
>Mature_506_residues
MKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRIANTIQNATVMSLARAKESDI
RRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDSIYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRA
GANVINIPDTVGYTNPEEYYALFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL
EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVSKNKAIVGANAFAHESGIHQDGVLKEVTTYEIIEPALVGE
SQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFKKLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVS
NSTQCATVVLKDEEGNVFEDAATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA
QDVLEASARAYVHAAGKLKSFIQLVK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=493, Percent_Identity=45.2332657200811, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6320071, Length=366, Percent_Identity=28.9617486338798, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320019, Length=361, Percent_Identity=29.0858725761773, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6324225, Length=528, Percent_Identity=24.6212121212121, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6324682, Length=529, Percent_Identity=24.007561436673, Blast_Score=127, Evalue=4e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_BACAA (C3P4Z6)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002865912.1
- ProteinModelPortal:   C3P4Z6
- EnsemblBacteria:   EBBACT00000126391
- GeneID:   7848302
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_1487
- GeneTree:   EBGT00050000000890
- ProtClustDB:   PRK00915
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 55375; Mature: 55375

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRI
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHHH
ANTIQNATVMSLARAKESDIRRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEHHHHCCHHHHHHH
IYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRAGANVINIPDTVGYTNPEEYY
HHHHHHCCCHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHH
ALFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL
HHHHHHHHHCCHHHHHHEEEECCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCHHHH
EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVSKNKAIVGANAFAHESGIHQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCEEEECCHHHHCCCCCC
DGVLKEVTTYEIIEPALVGESQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFK
CCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
KLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVSNSTQCATVVLKDEEGNVFED
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCEECC
AATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHEEEEEEHHHCHHHHCCCHHH
QDVLEASARAYVHAAGKLKSFIQLVK
HHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRI
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHHH
ANTIQNATVMSLARAKESDIRRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEHHHHCCHHHHHHH
IYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRAGANVINIPDTVGYTNPEEYY
HHHHHHCCCHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHH
ALFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL
HHHHHHHHHCCHHHHHHEEEECCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCHHHH
EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVSKNKAIVGANAFAHESGIHQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCEEEECCHHHHCCCCCC
DGVLKEVTTYEIIEPALVGESQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFK
CCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
KLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVSNSTQCATVVLKDEEGNVFED
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCEECC
AATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHEEEEEEHHHCHHHHCCCHHH
QDVLEASARAYVHAAGKLKSFIQLVK
HHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA