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The map label for this gene is flhF [H]

Identifier: 227815456

GI number: 227815456

Start: 2622009

End: 2623316

Strand: Reverse

Name: flhF [H]

Synonym: BAMEG_2869

Alternate gene names: 227815456

Gene position: 2623316-2622009 (Counterclockwise)

Preceding gene: 227815457

Following gene: 227815455

Centisome position: 50.16

GC content: 36.24

Gene sequence:

>1308_bases
ATGGAAAGTACAGAAAAGAAAGAAGCGTTAATGCGAATAAAAGCAGCTTCGAAAAATGAATTGTATCGAAAGTTATTTGA
CCAATATGGTACAGATTATTATTACGTTGTCGATGAAAGTGTAAAACGCAATATCCCGTTTTTTTGGAAAAAGAATTATG
AAATGTTAGTTGCTTTTCCAGAGGATAAACAGGAAGAAGTGAACGAAGGTACAGCTCAATTTCATGAGCAATTAATGGAT
GTTGTGAATGACCCTTCAGAACAAATTGTGAAGGCGAACGGAATTCAATCGGTACTGCACAATTTAGAAAACGTGACGAC
TCCTATGTCATATGCGGCGATGCAAACAGGAAATAGTGAAGAATGGGCAAGAAAGAAAGAGAAACTATTAAAGCTGTTTG
AAAAAGGTATCGTCGTTGTGAAACAGACGGAAGAAACAAAAGTAACGAAAAAGCAAAAAGCTGTGAAAAAAGTCGTTCCT
GTCAAGAAAGAAGAAGTGGTTGTAAAAAAAGAAAAGCAAGAATCTGTACCATTTATTATTCAAAAAGTAATTCGCATGCT
AGAGCAAAACGATGTAGAACAATACTTCATTCATGCCTATGCTGAAAAGCTAAAAGTGAAGTTTGAGAATGCAACGATGA
TTACAGAAGAAGTAATCGAATATATATTGGAAGATATGAGCTCTCATTTCAACACAGAAAACGTATTTGAAAAAGAAGTG
CAAACAATTGCATTAATTGGCCCAACTGGTGTTGGAAAAACGACGACACTTGCGAAAATGGCGTGGCAGTTTCACGGTAA
GAAAAAAACGGTTGGTTTTATTACGACAGACCATTCGCGCATTGGAACAGTGCAGCAACTGCAAGATTACGTAAAGACAA
TTGGATTTGAAGTAATCGCTGTACGTGATGAAGCTGCAATGACAAGAGCGCTTACGTATTTTAAAGAAGAAGCGCGCGTC
GATTATATTTTAATTGATACAGCTGGGAAAAATTATCGTGCGTCAGAAACAGTGGAAGAAATGATTGAAACGATGGGACA
AGTAGAGCCAGATTATATTTGTTTAACGTTATCAGCTTCGATGAAAAGTAAAGATATGATTGAAATCATTACGAACTTTA
AAGATATTCATATTGATGGTATCGTGTTTACGAAATTTGATGAAACGGCAAGTAGTGGTGAACTGTTGAAAATTCCAGCA
GTATCATCAGCTCCCATTGTATTAATGACAGACGGACAAGACGTGAAGAAAAACATACATATCGCTACAGCTGAACATTT
AGCGAAACAAATGTTGCAAACATCATAG

Upstream 100 bases:

>100_bases
AGCGCTATCAAGTAGAAGCGCAAGTACTTTGTATTAGCGAATTAGCACCAGAAATTGTTGTAGATCAAATTGCCTATATT
GAATAGTAGGGGGTGAAGTA

Downstream 100 bases:

>100_bases
AAAAGAGGTGAAGGATAAGGCAACTGCCTTATTCAAACGAGTATGAACGGTCTTTATATAGGTTCTATGGGTATGATGAA
TTACATGCAGCGCATTAATG

Product: flagellar biosynthesis regulator FlhF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MESTEKKEALMRIKAASKNELYRKLFDQYGTDYYYVVDESVKRNIPFFWKKNYEMLVAFPEDKQEEVNEGTAQFHEQLMD
VVNDPSEQIVKANGIQSVLHNLENVTTPMSYAAMQTGNSEEWARKKEKLLKLFEKGIVVVKQTEETKVTKKQKAVKKVVP
VKKEEVVVKKEKQESVPFIIQKVIRMLEQNDVEQYFIHAYAEKLKVKFENATMITEEVIEYILEDMSSHFNTENVFEKEV
QTIALIGPTGVGKTTTLAKMAWQFHGKKKTVGFITTDHSRIGTVQQLQDYVKTIGFEVIAVRDEAAMTRALTYFKEEARV
DYILIDTAGKNYRASETVEEMIETMGQVEPDYICLTLSASMKSKDMIEIITNFKDIHIDGIVFTKFDETASSGELLKIPA
VSSAPIVLMTDGQDVKKNIHIATAEHLAKQMLQTS

Sequences:

>Translated_435_residues
MESTEKKEALMRIKAASKNELYRKLFDQYGTDYYYVVDESVKRNIPFFWKKNYEMLVAFPEDKQEEVNEGTAQFHEQLMD
VVNDPSEQIVKANGIQSVLHNLENVTTPMSYAAMQTGNSEEWARKKEKLLKLFEKGIVVVKQTEETKVTKKQKAVKKVVP
VKKEEVVVKKEKQESVPFIIQKVIRMLEQNDVEQYFIHAYAEKLKVKFENATMITEEVIEYILEDMSSHFNTENVFEKEV
QTIALIGPTGVGKTTTLAKMAWQFHGKKKTVGFITTDHSRIGTVQQLQDYVKTIGFEVIAVRDEAAMTRALTYFKEEARV
DYILIDTAGKNYRASETVEEMIETMGQVEPDYICLTLSASMKSKDMIEIITNFKDIHIDGIVFTKFDETASSGELLKIPA
VSSAPIVLMTDGQDVKKNIHIATAEHLAKQMLQTS
>Mature_435_residues
MESTEKKEALMRIKAASKNELYRKLFDQYGTDYYYVVDESVKRNIPFFWKKNYEMLVAFPEDKQEEVNEGTAQFHEQLMD
VVNDPSEQIVKANGIQSVLHNLENVTTPMSYAAMQTGNSEEWARKKEKLLKLFEKGIVVVKQTEETKVTKKQKAVKKVVP
VKKEEVVVKKEKQESVPFIIQKVIRMLEQNDVEQYFIHAYAEKLKVKFENATMITEEVIEYILEDMSSHFNTENVFEKEV
QTIALIGPTGVGKTTTLAKMAWQFHGKKKTVGFITTDHSRIGTVQQLQDYVKTIGFEVIAVRDEAAMTRALTYFKEEARV
DYILIDTAGKNYRASETVEEMIETMGQVEPDYICLTLSASMKSKDMIEIITNFKDIHIDGIVFTKFDETASSGELLKIPA
VSSAPIVLMTDGQDVKKNIHIATAEHLAKQMLQTS

Specific function: Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum [H]

COG id: COG1419

COG function: function code N; Flagellar GTP-binding protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding SRP family [H]

Homologues:

Organism=Escherichia coli, GI1789874, Length=213, Percent_Identity=27.6995305164319, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325345, Length=240, Percent_Identity=23.3333333333333, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR000897
- InterPro:   IPR020006 [H]

Pfam domain/function: PF00448 SRP54 [H]

EC number: NA

Molecular weight: Translated: 49738; Mature: 49738

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESTEKKEALMRIKAASKNELYRKLFDQYGTDYYYVVDESVKRNIPFFWKKNYEMLVAFP
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCHHHCCCCEEEECCCEEEEECC
EDKQEEVNEGTAQFHEQLMDVVNDPSEQIVKANGIQSVLHNLENVTTPMSYAAMQTGNSE
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHCCCCCH
EWARKKEKLLKLFEKGIVVVKQTEETKVTKKQKAVKKVVPVKKEEVVVKKEKQESVPFII
HHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHH
QKVIRMLEQNDVEQYFIHAYAEKLKVKFENATMITEEVIEYILEDMSSHFNTENVFEKEV
HHHHHHHHHCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QTIALIGPTGVGKTTTLAKMAWQFHGKKKTVGFITTDHSRIGTVQQLQDYVKTIGFEVIA
HEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCHHCCCHHHHHHHHHHCCEEEEE
VRDEAAMTRALTYFKEEARVDYILIDTAGKNYRASETVEEMIETMGQVEPDYICLTLSAS
ECCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECC
MKSKDMIEIITNFKDIHIDGIVFTKFDETASSGELLKIPAVSSAPIVLMTDGQDVKKNIH
CCCCHHHHHHHCCCCEEECEEEEEECCCCCCCCCEEEECCCCCCCEEEEECCCHHHHCEE
IATAEHLAKQMLQTS
EEHHHHHHHHHHHCC
>Mature Secondary Structure
MESTEKKEALMRIKAASKNELYRKLFDQYGTDYYYVVDESVKRNIPFFWKKNYEMLVAFP
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCHHHCCCCEEEECCCEEEEECC
EDKQEEVNEGTAQFHEQLMDVVNDPSEQIVKANGIQSVLHNLENVTTPMSYAAMQTGNSE
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHCCCCCH
EWARKKEKLLKLFEKGIVVVKQTEETKVTKKQKAVKKVVPVKKEEVVVKKEKQESVPFII
HHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHH
QKVIRMLEQNDVEQYFIHAYAEKLKVKFENATMITEEVIEYILEDMSSHFNTENVFEKEV
HHHHHHHHHCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QTIALIGPTGVGKTTTLAKMAWQFHGKKKTVGFITTDHSRIGTVQQLQDYVKTIGFEVIA
HEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCHHCCCHHHHHHHHHHCCEEEEE
VRDEAAMTRALTYFKEEARVDYILIDTAGKNYRASETVEEMIETMGQVEPDYICLTLSAS
ECCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECC
MKSKDMIEIITNFKDIHIDGIVFTKFDETASSGELLKIPAVSSAPIVLMTDGQDVKKNIH
CCCCHHHHHHHCCCCEEECEEEEEECCCCCCCCCEEEECCCCCCCEEEEECCCHHHHCEE
IATAEHLAKQMLQTS
EEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]