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The map label for this gene is rocR2 [H]

Identifier: 227814884

GI number: 227814884

Start: 2091693

End: 2093072

Strand: Reverse

Name: rocR2 [H]

Synonym: BAMEG_2295

Alternate gene names: 227814884

Gene position: 2093072-2091693 (Counterclockwise)

Preceding gene: 227814885

Following gene: 227814883

Centisome position: 40.02

GC content: 36.3

Gene sequence:

>1380_bases
TTGCTAGCAGTTTCGACACAAGAAGTCATTGAAGCGATTTTGGGCAGTATTGATGAGGCTATACACGCAGTAGATGAAAA
TGGTATTACAATTTTCTATAATACTGTAGCTGCAAAACACGATGGATCAAAAATTGAAAAAGTGTTAGGTAAACATTTGT
TAGAAGCGTTCCCTTCTTTATCTAGGGAAACAAGTACGTTGATGAAAGTGTTAGATACAAAAAAACCAATCGTTCATCAA
GTGCAGCATTATCAAAATTTAAATGGAGAAGACATTTGCACAGTAAATACGACGTTACCTATTTTTATAGATGGAAATAT
TGCTGGAGCTGTTGAAATTGCAAAGGATTATTCCACAGTTCAAAAGCTTACAGATACAATAGTCGATTTGCAGTCGAAAA
TAAAGCGGTCATCTAGAAAAAAAGCAATCAAAAAGCATGTTGCATTTGAGACAATCGTAACGAATGACGCTCGGTTTAAA
CAGACGAAAGAATTAGCACAAAAAGTAGCTCCAACTGATGCGAATGTTTTAATATATGGGGAAACAGGAACAGGGAAAGA
GTTATTTGTACAAGCGATTCATGAAGCTTCTAAACGGAAAAACAAGCCGTTTATTGCACAAAACTGCGCAGCTTTGCCAG
AGTCTCTATTAGAAAGTTTATTGTTTGGAACGACGAAGGGTAGTTATACTGGGGCAATTGAACGAGCGGGGTTATTTGAA
CTTGTAGATGGAGGGACATTGTTTTTAGATGAACTGAATTCAATGCCACTCGATTTACAAGCGAAAATGCTACGTGTATT
AGAAGATGGTGTTATTCGTCGTATTGGTGATAATAAGACGAGAAAAGTAGATGTTCGCGTTATTACAGCAATGAATCAGC
CGCCAGAAGTATGTTTAAAAGAGAATAAAATTCGCACTGATTTATACTATCGATTAAATGTCTTTTCATTATATATTCCG
CCGCTCCGTGAAAGAACGGAGGACGTATTATTATTAGCGTCTTATTTTTTGAAAGAATATAATAAAAGTTATAAAAAAGG
TGTACTTCAAATGGATAAGGAAGCGAAAGAAAGACTACAATCTTACCAATGGCCTGGAAATGTTCGGGAGCTAAAACATA
CGATTGAACATGCTGTCATTATTGCAGAAGGGAATACATTAACAGCCAATTGTTTACCGCGTACATTTCGGAAAGAGAGT
TTTCCGAAGAAGAAAAGCATATTGCCGCTTAGAGAAGCACTTCATCAAACAGAAAAAGAATTAATAGATCAAGCGTTAAT
TGAAACGGAAGGGAATATACTACAAGCTGCAAAAATGTTAGGCATCCCTCGTCAAACGCTTCAATATAAACTGAGTAAGT
ACGACAAAACCGCCGAATAA

Upstream 100 bases:

>100_bases
TATAAAAATAAAACGGAACCGTCATGCTTATAATCGCACATAGGTATGCCGGAAATTCGGCATAGTATTTGCTGAATTTT
CGGCAAGAGGGGGGATGACA

Downstream 100 bases:

>100_bases
TTGGCGGTTTTTGTGTGTTTGCACGAATAAAAGAGCGTTTACATATATGTAAAGTAATGAATATGATAGTATGTATGAAT
TTCATACAGTGCTTAAAAAA

Product: arginine utilization regulatory protein RocR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLAVSTQEVIEAILGSIDEAIHAVDENGITIFYNTVAAKHDGSKIEKVLGKHLLEAFPSLSRETSTLMKVLDTKKPIVHQ
VQHYQNLNGEDICTVNTTLPIFIDGNIAGAVEIAKDYSTVQKLTDTIVDLQSKIKRSSRKKAIKKHVAFETIVTNDARFK
QTKELAQKVAPTDANVLIYGETGTGKELFVQAIHEASKRKNKPFIAQNCAALPESLLESLLFGTTKGSYTGAIERAGLFE
LVDGGTLFLDELNSMPLDLQAKMLRVLEDGVIRRIGDNKTRKVDVRVITAMNQPPEVCLKENKIRTDLYYRLNVFSLYIP
PLRERTEDVLLLASYFLKEYNKSYKKGVLQMDKEAKERLQSYQWPGNVRELKHTIEHAVIIAEGNTLTANCLPRTFRKES
FPKKKSILPLREALHQTEKELIDQALIETEGNILQAAKMLGIPRQTLQYKLSKYDKTAE

Sequences:

>Translated_459_residues
MLAVSTQEVIEAILGSIDEAIHAVDENGITIFYNTVAAKHDGSKIEKVLGKHLLEAFPSLSRETSTLMKVLDTKKPIVHQ
VQHYQNLNGEDICTVNTTLPIFIDGNIAGAVEIAKDYSTVQKLTDTIVDLQSKIKRSSRKKAIKKHVAFETIVTNDARFK
QTKELAQKVAPTDANVLIYGETGTGKELFVQAIHEASKRKNKPFIAQNCAALPESLLESLLFGTTKGSYTGAIERAGLFE
LVDGGTLFLDELNSMPLDLQAKMLRVLEDGVIRRIGDNKTRKVDVRVITAMNQPPEVCLKENKIRTDLYYRLNVFSLYIP
PLRERTEDVLLLASYFLKEYNKSYKKGVLQMDKEAKERLQSYQWPGNVRELKHTIEHAVIIAEGNTLTANCLPRTFRKES
FPKKKSILPLREALHQTEKELIDQALIETEGNILQAAKMLGIPRQTLQYKLSKYDKTAE
>Mature_459_residues
MLAVSTQEVIEAILGSIDEAIHAVDENGITIFYNTVAAKHDGSKIEKVLGKHLLEAFPSLSRETSTLMKVLDTKKPIVHQ
VQHYQNLNGEDICTVNTTLPIFIDGNIAGAVEIAKDYSTVQKLTDTIVDLQSKIKRSSRKKAIKKHVAFETIVTNDARFK
QTKELAQKVAPTDANVLIYGETGTGKELFVQAIHEASKRKNKPFIAQNCAALPESLLESLLFGTTKGSYTGAIERAGLFE
LVDGGTLFLDELNSMPLDLQAKMLRVLEDGVIRRIGDNKTRKVDVRVITAMNQPPEVCLKENKIRTDLYYRLNVFSLYIP
PLRERTEDVLLLASYFLKEYNKSYKKGVLQMDKEAKERLQSYQWPGNVRELKHTIEHAVIIAEGNTLTANCLPRTFRKES
FPKKKSILPLREALHQTEKELIDQALIETEGNILQAAKMLGIPRQTLQYKLSKYDKTAE

Specific function: Positive regulator of arginine catabolism. Controls the transcription of the two operons rocABC and rocDEF and probably acts by binding to the corresponding upstream activating sequences [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=341, Percent_Identity=43.108504398827, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI87082117, Length=437, Percent_Identity=35.4691075514874, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1788905, Length=311, Percent_Identity=42.7652733118971, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1790437, Length=288, Percent_Identity=44.0972222222222, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI87082152, Length=291, Percent_Identity=45.360824742268, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789087, Length=371, Percent_Identity=38.2749326145553, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=39.8753894080997, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1790299, Length=308, Percent_Identity=39.9350649350649, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1786524, Length=328, Percent_Identity=37.1951219512195, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87081872, Length=311, Percent_Identity=38.2636655948553, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1787583, Length=275, Percent_Identity=38.5454545454545, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI87081858, Length=309, Percent_Identity=31.3915857605178, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1789828, Length=319, Percent_Identity=29.153605015674, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF08448 PAS_4; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51639; Mature: 51639

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAVSTQEVIEAILGSIDEAIHAVDENGITIFYNTVAAKHDGSKIEKVLGKHLLEAFPSL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
SRETSTLMKVLDTKKPIVHQVQHYQNLNGEDICTVNTTLPIFIDGNIAGAVEIAKDYSTV
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECEEEEEECCCCCCHHHHHHHHHHH
QKLTDTIVDLQSKIKRSSRKKAIKKHVAFETIVTNDARFKQTKELAQKVAPTDANVLIYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEE
ETGTGKELFVQAIHEASKRKNKPFIAQNCAALPESLLESLLFGTTKGSYTGAIERAGLFE
CCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHE
LVDGGTLFLDELNSMPLDLQAKMLRVLEDGVIRRIGDNKTRKVDVRVITAMNQPPEVCLK
EECCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHC
ENKIRTDLYYRLNVFSLYIPPLRERTEDVLLLASYFLKEYNKSYKKGVLQMDKEAKERLQ
CCCCCCHHEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYQWPGNVRELKHTIEHAVIIAEGNTLTANCLPRTFRKESFPKKKSILPLREALHQTEKE
HCCCCCCHHHHHHHHHHEEEEECCCEEEHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LIDQALIETEGNILQAAKMLGIPRQTLQYKLSKYDKTAE
HHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLAVSTQEVIEAILGSIDEAIHAVDENGITIFYNTVAAKHDGSKIEKVLGKHLLEAFPSL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
SRETSTLMKVLDTKKPIVHQVQHYQNLNGEDICTVNTTLPIFIDGNIAGAVEIAKDYSTV
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECEEEEEECCCCCCHHHHHHHHHHH
QKLTDTIVDLQSKIKRSSRKKAIKKHVAFETIVTNDARFKQTKELAQKVAPTDANVLIYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEE
ETGTGKELFVQAIHEASKRKNKPFIAQNCAALPESLLESLLFGTTKGSYTGAIERAGLFE
CCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHE
LVDGGTLFLDELNSMPLDLQAKMLRVLEDGVIRRIGDNKTRKVDVRVITAMNQPPEVCLK
EECCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHC
ENKIRTDLYYRLNVFSLYIPPLRERTEDVLLLASYFLKEYNKSYKKGVLQMDKEAKERLQ
CCCCCCHHEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYQWPGNVRELKHTIEHAVIIAEGNTLTANCLPRTFRKESFPKKKSILPLREALHQTEKE
HCCCCCCHHHHHHHHHHEEEEECCCEEEHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LIDQALIETEGNILQAAKMLGIPRQTLQYKLSKYDKTAE
HHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113162; 9384377 [H]