Click here to switch to the map view.

The map label for this gene is murE [H]

Identifier: 227814596

GI number: 227814596

Start: 1824229

End: 1825434

Strand: Reverse

Name: murE [H]

Synonym: BAMEG_2006

Alternate gene names: 227814596

Gene position: 1825434-1824229 (Counterclockwise)

Preceding gene: 227814597

Following gene: 227814595

Centisome position: 34.9

GC content: 32.92

Gene sequence:

>1206_bases
TTGAAACTAAAAGAGCTGGCGAATTTGTTATTGGTTAAAGAAACTATTGGCGATATGAATGTAAAAATTACGGGATTAGA
AATGGATTCTAGAAAGATAACTAATGGTAACCTATTTATATGTGTATCAGGTATCGATGGTTTTCTTGAAGATCGACATC
AATTTGTTGAAGATGCAGTGAAAAATGGTGCTGTAGCGCTAATTGTAGAAAGAGATGTAAATATTGAGATACCTAAAATA
ATTGTTAAAGATGCTAGGTATGCTATGGCAGTTATTGCGTCACATTTCTATGGTTATCCATCTAATAAAATGAAGTTAAT
TGGAATTACAGGGACAAATGGGAAAACAACTACTTCCTATTTACTTGAAAAAATTTTAAGTGATTATGGATATCACACGG
GGCTCATGGGGAATAACGGAGTGAAAGTGGGTTCAAAATGGTATCCTACTGATATTAATACGCAAGAACCTCCAACACTT
CAACGGAATTTGCAAATGATGAAAAATCAAAATGCAGATTATTGTGTTATGGAAGTTACGTCACAAGGGTTACATATGGA
AAGAGTTAAAGGGTGTAACTTTAAAATAGCTGTTTTCACAAATTTAACACAAGATCATTTAGATTATCATGGAACATTTG
ATGAATATAAGCATGTAAAAAGTCTTCTTTTTGCGAGATTAGGAAATAATTTATCTACTATAGATAAAAAAATAGCTGTT
TTGAATGCAGACGATCCATCGGTAGAATACTTTAAAAAAATTACTTCAGCTGAGGTTATCACATATGGAATACATAATGG
AGCAGATGTTCAAGCGAAGAACATAACTTTAAGTCCAAAAGGAATTCGTTTTTTAGTTTCGTCCTTTAATGGCGAAATAG
AGGTTAGCCTTAATCTTGTAGGTCGTTTTAATGTTTATAATGCATTAGCAGCAATAACAGTTGCATTGGTTGAAGGTATT
CCGCTTACAAACATTAGTGATAGCTTATCTAACTTAAAGAGTATTGAAGGTAGAATGGAAATCATAGATGAAAATCAAGA
TTTCCTTGTAATAGTGGACTATGCTCATACACCGGATGCGTTAGAAAATGTTCTCTCTACAATTAGAGAGTTTGCAAAAG
GTAAAATTATTACTGTTTTCGGTTGTGGTGGAGATAGAGACGCAAAAAAAACGATCTATAATGGGTGGAATTGCTGGAAG
TTATAG

Upstream 100 bases:

>100_bases
AAGGGTGATTAAATTTAGTTCAAAAAATATGGAATTTTAAACCATTGTTTTTATACAAAGTGGTAAACTTAATAAGGTAA
TATGTAAGGGGGCGGGGGAT

Downstream 100 bases:

>100_bases
TGATTTTGTTTTTATTACTTCTGATAATCCACGTTCGGAAGATCCGCAGGCAATAATGAAAGATATTGAAAAAGGATTTT
CGCAAAATAATAATTTGAAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKLKELANLLLVKETIGDMNVKITGLEMDSRKITNGNLFICVSGIDGFLEDRHQFVEDAVKNGAVALIVERDVNIEIPKI
IVKDARYAMAVIASHFYGYPSNKMKLIGITGTNGKTTTSYLLEKILSDYGYHTGLMGNNGVKVGSKWYPTDINTQEPPTL
QRNLQMMKNQNADYCVMEVTSQGLHMERVKGCNFKIAVFTNLTQDHLDYHGTFDEYKHVKSLLFARLGNNLSTIDKKIAV
LNADDPSVEYFKKITSAEVITYGIHNGADVQAKNITLSPKGIRFLVSSFNGEIEVSLNLVGRFNVYNALAAITVALVEGI
PLTNISDSLSNLKSIEGRMEIIDENQDFLVIVDYAHTPDALENVLSTIREFAKGKIITVFGCGGDRDAKKTIYNGWNCWK
L

Sequences:

>Translated_401_residues
MKLKELANLLLVKETIGDMNVKITGLEMDSRKITNGNLFICVSGIDGFLEDRHQFVEDAVKNGAVALIVERDVNIEIPKI
IVKDARYAMAVIASHFYGYPSNKMKLIGITGTNGKTTTSYLLEKILSDYGYHTGLMGNNGVKVGSKWYPTDINTQEPPTL
QRNLQMMKNQNADYCVMEVTSQGLHMERVKGCNFKIAVFTNLTQDHLDYHGTFDEYKHVKSLLFARLGNNLSTIDKKIAV
LNADDPSVEYFKKITSAEVITYGIHNGADVQAKNITLSPKGIRFLVSSFNGEIEVSLNLVGRFNVYNALAAITVALVEGI
PLTNISDSLSNLKSIEGRMEIIDENQDFLVIVDYAHTPDALENVLSTIREFAKGKIITVFGCGGDRDAKKTIYNGWNCWK
L
>Mature_401_residues
MKLKELANLLLVKETIGDMNVKITGLEMDSRKITNGNLFICVSGIDGFLEDRHQFVEDAVKNGAVALIVERDVNIEIPKI
IVKDARYAMAVIASHFYGYPSNKMKLIGITGTNGKTTTSYLLEKILSDYGYHTGLMGNNGVKVGSKWYPTDINTQEPPTL
QRNLQMMKNQNADYCVMEVTSQGLHMERVKGCNFKIAVFTNLTQDHLDYHGTFDEYKHVKSLLFARLGNNLSTIDKKIAV
LNADDPSVEYFKKITSAEVITYGIHNGADVQAKNITLSPKGIRFLVSSFNGEIEVSLNLVGRFNVYNALAAITVALVEGI
PLTNISDSLSNLKSIEGRMEIIDENQDFLVIVDYAHTPDALENVLSTIREFAKGKIITVFGCGGDRDAKKTIYNGWNCWK
L

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=387, Percent_Identity=35.6589147286822, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI1786274, Length=339, Percent_Identity=30.0884955752212, Blast_Score=102, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 44593; Mature: 44593

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKELANLLLVKETIGDMNVKITGLEMDSRKITNGNLFICVSGIDGFLEDRHQFVEDAV
CCHHHHHHHHHHHHHHCCCEEEEEEEEECCCEECCCCEEEEEECCCHHHHHHHHHHHHHH
KNGAVALIVERDVNIEIPKIIVKDARYAMAVIASHFYGYPSNKMKLIGITGTNGKTTTSY
CCCCEEEEEECCCCEECCHHEEHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHH
LLEKILSDYGYHTGLMGNNGVKVGSKWYPTDINTQEPPTLQRNLQMMKNQNADYCVMEVT
HHHHHHHHCCCCCCCCCCCCEEECCEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEC
SQGLHMERVKGCNFKIAVFTNLTQDHLDYHGTFDEYKHVKSLLFARLGNNLSTIDKKIAV
CCCCCHHHHCCCCEEEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHCCEEEE
LNADDPSVEYFKKITSAEVITYGIHNGADVQAKNITLSPKGIRFLVSSFNGEIEVSLNLV
EECCCCCHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCHHHHHEECCCCEEEEEEEEE
GRFNVYNALAAITVALVEGIPLTNISDSLSNLKSIEGRMEIIDENQDFLVIVDYAHTPDA
EECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCHH
LENVLSTIREFAKGKIITVFGCGGDRDAKKTIYNGWNCWKL
HHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHCCEEEECC
>Mature Secondary Structure
MKLKELANLLLVKETIGDMNVKITGLEMDSRKITNGNLFICVSGIDGFLEDRHQFVEDAV
CCHHHHHHHHHHHHHHCCCEEEEEEEEECCCEECCCCEEEEEECCCHHHHHHHHHHHHHH
KNGAVALIVERDVNIEIPKIIVKDARYAMAVIASHFYGYPSNKMKLIGITGTNGKTTTSY
CCCCEEEEEECCCCEECCHHEEHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHH
LLEKILSDYGYHTGLMGNNGVKVGSKWYPTDINTQEPPTLQRNLQMMKNQNADYCVMEVT
HHHHHHHHCCCCCCCCCCCCEEECCEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEC
SQGLHMERVKGCNFKIAVFTNLTQDHLDYHGTFDEYKHVKSLLFARLGNNLSTIDKKIAV
CCCCCHHHHCCCCEEEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHCCEEEE
LNADDPSVEYFKKITSAEVITYGIHNGADVQAKNITLSPKGIRFLVSSFNGEIEVSLNLV
EECCCCCHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCHHHHHEECCCCEEEEEEEEE
GRFNVYNALAAITVALVEGIPLTNISDSLSNLKSIEGRMEIIDENQDFLVIVDYAHTPDA
EECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCHH
LENVLSTIREFAKGKIITVFGCGGDRDAKKTIYNGWNCWKL
HHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436954; 9384377; 8289242 [H]