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The map label for this gene is dnaN2 [H]

Identifier: 227814502

GI number: 227814502

Start: 1737949

End: 1739079

Strand: Reverse

Name: dnaN2 [H]

Synonym: BAMEG_1910

Alternate gene names: 227814502

Gene position: 1739079-1737949 (Counterclockwise)

Preceding gene: 227814503

Following gene: 227814501

Centisome position: 33.25

GC content: 31.92

Gene sequence:

>1131_bases
ATGGAGTTTATCGTTAATCACAAACATTTTACACAAGCACTTTCAGATGTAAGTAAAGCAATATCAACGAAAGCTATAAT
TCCTATATTATCTGGCATAAAAATAACAGCAGATCAATCTGGGATTACTTTAATCGCAAGTAATTCGAATATTTTCATCG
AAAAATTTATACCTAGTGCTATAGATGACGAACAAATTACGACTATCTTACAAGCAGGAACAATTGTTGTACCTGCAAAA
TACTTCATTGAAATTATAAAGAAAATGCCAAGCGATATAGTAATAAAAAGTAAGAATGAGCAAACAATTACCATACAATC
AGGAGAAATCACATTAAACTTAAATGGCTTTCCAGCGAATGAATTTCCTAACGTACCACAAATAGACGATCATACAGAAA
TACAAATAGAAACAAAACAATTGATTGATGCGTTTAAACAAACAGTCTTTGCAGTAGCGAAAAATGAATCTAGACACGTT
CTTACTGGCGTACATATCGAGTTAGACCATAACAAATTAATATGCGCTGCAACTGACTCTCATAGATTAGCTATACGTGA
AACACTAATTTCTACTAATATGAAAGCGAATTGTATTGTACCAAGTGCAACCATTAATGAGCTTTTAAAATTAATGAACA
GCAATTTAGAATTCGTTTCTATTTATCTATCAGAAAGTCACATTATTTTTACATTCGGCACAACTACGTTATATTCACGA
CTAATTGAAGGGAAATATCCTAATATTTCTACTCTCATTCCAAATGAATTTCAAACCGTCATTAACATAGATAGACAAAG
AATGTTACAAGGGGTAGATCGATCAAGCTTATTAGCGAGTGAATGGGCAAATAACAATGTTAACTTAGAAATTGTAAACG
AATCTACAATTCAAATTTCTTCTAACGCTTCTCAGATCGGAAAAATATCTGAGAAGCAACAGATAGATGTAATTCAAGGT
AAAAAACAATTAAATATATCTTTCGATGGACGATTTATGTTGGATGCTTTAAGGGCAATAAAAGAAGAAACGGTTACTTT
AAGTTTCAGCGGTTCTATGAGACCAATATTAATTGAAGCAGGGACACAATCTGCAGCAATCCATCTTATATCTCCAGTAA
GAGCTTATTAA

Upstream 100 bases:

>100_bases
AATATGTTAAAAAAGAAAGAAACGGTACCTTACTTTTTTAACGGAACTATCGTTCATCAATCTTCATAAAAATATTATTT
CATTTTAGGTGGGGGAAAAG

Downstream 100 bases:

>100_bases
AAACAGAGGAAGGAATCACATTATGTACAAACACACTTTATGTTTCATAAAAAGAAATGAAGAGATACTTATGCTAAATA
GAAAATATGACCCTGTAAAA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MEFIVNHKHFTQALSDVSKAISTKAIIPILSGIKITADQSGITLIASNSNIFIEKFIPSAIDDEQITTILQAGTIVVPAK
YFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPANEFPNVPQIDDHTEIQIETKQLIDAFKQTVFAVAKNESRHV
LTGVHIELDHNKLICAATDSHRLAIRETLISTNMKANCIVPSATINELLKLMNSNLEFVSIYLSESHIIFTFGTTTLYSR
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASEWANNNVNLEIVNESTIQISSNASQIGKISEKQQIDVIQG
KKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEAGTQSAAIHLISPVRAY

Sequences:

>Translated_376_residues
MEFIVNHKHFTQALSDVSKAISTKAIIPILSGIKITADQSGITLIASNSNIFIEKFIPSAIDDEQITTILQAGTIVVPAK
YFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPANEFPNVPQIDDHTEIQIETKQLIDAFKQTVFAVAKNESRHV
LTGVHIELDHNKLICAATDSHRLAIRETLISTNMKANCIVPSATINELLKLMNSNLEFVSIYLSESHIIFTFGTTTLYSR
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASEWANNNVNLEIVNESTIQISSNASQIGKISEKQQIDVIQG
KKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEAGTQSAAIHLISPVRAY
>Mature_376_residues
MEFIVNHKHFTQALSDVSKAISTKAIIPILSGIKITADQSGITLIASNSNIFIEKFIPSAIDDEQITTILQAGTIVVPAK
YFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPANEFPNVPQIDDHTEIQIETKQLIDAFKQTVFAVAKNESRHV
LTGVHIELDHNKLICAATDSHRLAIRETLISTNMKANCIVPSATINELLKLMNSNLEFVSIYLSESHIIFTFGTTTLYSR
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASEWANNNVNLEIVNESTIQISSNASQIGKISEKQQIDVIQG
KKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEAGTQSAAIHLISPVRAY

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=380, Percent_Identity=24.2105263157895, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41706; Mature: 41706

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFIVNHKHFTQALSDVSKAISTKAIIPILSGIKITADQSGITLIASNSNIFIEKFIPSA
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCEEEEECCCCC
IDDEQITTILQAGTIVVPAKYFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPAN
CCHHHHHHHHHCCCEEECHHHHHHHHHHCCCCEEEECCCCCEEEEECCEEEEEECCCCCC
EFPNVPQIDDHTEIQIETKQLIDAFKQTVFAVAKNESRHVLTGVHIELDHNKLICAATDS
CCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHEECCCCCEEEEEEEEEECCCEEEEEECCC
HRLAIRETLISTNMKANCIVPSATINELLKLMNSNLEFVSIYLSESHIIFTFGTTTLYSR
CCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEEECCEEEEEECHHHHHHH
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASEWANNNVNLEIVNESTIQIS
HHCCCCCCCCEECCCHHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCEEEEE
SNASQIGKISEKQQIDVIQGKKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEA
CCHHHHHCCCCCCCCCEECCCEEEEEEECCHHHHHHHHHHHHCEEEEEECCCCCEEEEEC
GTQSAAIHLISPVRAY
CCCCEEEEEECCCCCC
>Mature Secondary Structure
MEFIVNHKHFTQALSDVSKAISTKAIIPILSGIKITADQSGITLIASNSNIFIEKFIPSA
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCEEEEECCCCC
IDDEQITTILQAGTIVVPAKYFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPAN
CCHHHHHHHHHCCCEEECHHHHHHHHHHCCCCEEEECCCCCEEEEECCEEEEEECCCCCC
EFPNVPQIDDHTEIQIETKQLIDAFKQTVFAVAKNESRHVLTGVHIELDHNKLICAATDS
CCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHEECCCCCEEEEEEEEEECCCEEEEEECCC
HRLAIRETLISTNMKANCIVPSATINELLKLMNSNLEFVSIYLSESHIIFTFGTTTLYSR
CCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEEECCEEEEEECHHHHHHH
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASEWANNNVNLEIVNESTIQIS
HHCCCCCCCCEECCCHHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCEEEEE
SNASQIGKISEKQQIDVIQGKKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEA
CCHHHHHCCCCCCCCCEECCCEEEEEEECCHHHHHHHHHHHHCEEEEEECCCCCEEEEEC
GTQSAAIHLISPVRAY
CCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]