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The map label for this gene is opuAB [H]

Identifier: 227814403

GI number: 227814403

Start: 1648961

End: 1649797

Strand: Direct

Name: opuAB [H]

Synonym: BAMEG_1811

Alternate gene names: 227814403

Gene position: 1648961-1649797 (Clockwise)

Preceding gene: 227814402

Following gene: 227814404

Centisome position: 31.53

GC content: 35.36

Gene sequence:

>837_bases
ATGAATAGTATTCCGCGTATTCCATTAGGGGAATGGGTTGATTCATTTGTTGCAAGTTTATATGAGCACTTTGAAGGCTT
ATTCCGAGGATTCTCTTATATTATTGGAGGATTCGTTGATTTACTCACTAATTTTTTAATAATAATACCGGCCATATTGA
TGATTATTTTACTGTGTTTCCTAATTTGGTACACAACGAGAAAAGTATCTTTAGTTATTTTTACACTGATCGGATTATTA
TTTATTTTAAATATTAACTACTGGGCACAGACGATGCAAACATTAGCACTCGTACTTACTTCCGTTATTATTTCAATTAT
TGTTGGAATTCCAATTGGGATATTAGCATCTCAAAATGAGCGTTTCTCAAAATTTTTAAAACCGACATTAGATTTTATGC
AGACAATGCCGGCTTTCGTATATCTGATTCCTGCGATTACATTTTTCGGAGTAGGAGTAGTACCTGGGATTATTGCATCA
GTAATCTTTGCAATGCCACCGACAATTCGTTTTACTGATTTAGGAATTAGACAAGTTCCAGAAGATTTAATAGAAGCAGC
GAATGCATTTGGTTCAACCGCATCACAAAAACTATTTAAAGTACAATTACCACTCGCAACTGGAACGATTATGGCTGGTG
TGAATCAAAGCATTATGCTTTCGTTATCAATGGTAGTAACAGCATCTCTTGTAGGAGCACCAGGTCTCGGCGTTGATGTA
TACCGTTCAGTAACACAAGTTAATATCGGAATGGGATTTGAAGCCGGACTAGCTATTGTTGTCATTGCGATTATATTAGA
CCGCATTACACAAGGATTTCATCAAAAAAGAAGATAA

Upstream 100 bases:

>100_bases
TTACCCGTAATTGATGAGAAGAAAAGACTTAGAGGAATCATTAAACGTGAAAGTGTCATTCAAGCTCTTGCAGGAAACAT
TGAGGAAGAGGTGAAAGACG

Downstream 100 bases:

>100_bases
CAAAAAAACACACTTTATTATACAAAGTGTGTTTTTTGTTTGAATAAGATTACATACGAACAATGACAGAACCGTCAGCT
GGGGCACAGATTTGGTTTTG

Product: glycine betaine/L-proline ABC transporter, permease protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MNSIPRIPLGEWVDSFVASLYEHFEGLFRGFSYIIGGFVDLLTNFLIIIPAILMIILLCFLIWYTTRKVSLVIFTLIGLL
FILNINYWAQTMQTLALVLTSVIISIIVGIPIGILASQNERFSKFLKPTLDFMQTMPAFVYLIPAITFFGVGVVPGIIAS
VIFAMPPTIRFTDLGIRQVPEDLIEAANAFGSTASQKLFKVQLPLATGTIMAGVNQSIMLSLSMVVTASLVGAPGLGVDV
YRSVTQVNIGMGFEAGLAIVVIAIILDRITQGFHQKRR

Sequences:

>Translated_278_residues
MNSIPRIPLGEWVDSFVASLYEHFEGLFRGFSYIIGGFVDLLTNFLIIIPAILMIILLCFLIWYTTRKVSLVIFTLIGLL
FILNINYWAQTMQTLALVLTSVIISIIVGIPIGILASQNERFSKFLKPTLDFMQTMPAFVYLIPAITFFGVGVVPGIIAS
VIFAMPPTIRFTDLGIRQVPEDLIEAANAFGSTASQKLFKVQLPLATGTIMAGVNQSIMLSLSMVVTASLVGAPGLGVDV
YRSVTQVNIGMGFEAGLAIVVIAIILDRITQGFHQKRR
>Mature_278_residues
MNSIPRIPLGEWVDSFVASLYEHFEGLFRGFSYIIGGFVDLLTNFLIIIPAILMIILLCFLIWYTTRKVSLVIFTLIGLL
FILNINYWAQTMQTLALVLTSVIISIIVGIPIGILASQNERFSKFLKPTLDFMQTMPAFVYLIPAITFFGVGVVPGIIAS
VIFAMPPTIRFTDLGIRQVPEDLIEAANAFGSTASQKLFKVQLPLATGTIMAGVNQSIMLSLSMVVTASLVGAPGLGVDV
YRSVTQVNIGMGFEAGLAIVVIAIILDRITQGFHQKRR

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=276, Percent_Identity=46.3768115942029, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1788451, Length=193, Percent_Identity=26.9430051813472, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788449, Length=181, Percent_Identity=28.7292817679558, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30451; Mature: 30451

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIPRIPLGEWVDSFVASLYEHFEGLFRGFSYIIGGFVDLLTNFLIIIPAILMIILLCF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIWYTTRKVSLVIFTLIGLLFILNINYWAQTMQTLALVLTSVIISIIVGIPIGILASQNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCH
RFSKFLKPTLDFMQTMPAFVYLIPAITFFGVGVVPGIIASVIFAMPPTIRFTDLGIRQVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHCC
EDLIEAANAFGSTASQKLFKVQLPLATGTIMAGVNQSIMLSLSMVVTASLVGAPGLGVDV
HHHHHHHHHHCCHHHHHHHHEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHH
YRSVTQVNIGMGFEAGLAIVVIAIILDRITQGFHQKRR
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNSIPRIPLGEWVDSFVASLYEHFEGLFRGFSYIIGGFVDLLTNFLIIIPAILMIILLCF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIWYTTRKVSLVIFTLIGLLFILNINYWAQTMQTLALVLTSVIISIIVGIPIGILASQNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCH
RFSKFLKPTLDFMQTMPAFVYLIPAITFFGVGVVPGIIASVIFAMPPTIRFTDLGIRQVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHCC
EDLIEAANAFGSTASQKLFKVQLPLATGTIMAGVNQSIMLSLSMVVTASLVGAPGLGVDV
HHHHHHHHHHCCHHHHHHHHEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHH
YRSVTQVNIGMGFEAGLAIVVIAIILDRITQGFHQKRR
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]