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The map label for this gene is 227814350

Identifier: 227814350

GI number: 227814350

Start: 1604076

End: 1604408

Strand: Reverse

Name: 227814350

Synonym: BAMEG_1758

Alternate gene names: NA

Gene position: 1604408-1604076 (Counterclockwise)

Preceding gene: 227814351

Following gene: 227814349

Centisome position: 30.68

GC content: 38.74

Gene sequence:

>333_bases
ATGAGTAGAATTACACATCACCCTATTTTAGGAAGTTTAAATAATAGCAATCACATCACTTTTCAATTTAACGGTCAACA
ATATGAAGCATATGAACATGAAACGATTGCGGCCGCTTTACTTGCAAACGGAATAAGAACATTAAGAGTGCACGAAGATA
ACGGGACGCCGCGAGGTATTTACTGTAATATCGGACATTGTTCTGAATGCCGCGTCACTGTAAACAATCAAGCAAACGTA
CGAGCATGCTTAACTGTTGTAGAAAACGGTATGGTTGTTGATAGTGGTAAACAGCATCCAAATATCGTTAGAGAGATGGT
GAAAAAGCGATGA

Upstream 100 bases:

>100_bases
TGATTGAACATGCAAATCCAGGCGTAACTACAAATGATATTCCATTGAAATATCAACCACCGGTACGAGCAGTTACTTTT
GGATCGGTAGGTGAAAGTAA

Downstream 100 bases:

>100_bases
ATGATGTCATAATTATCGGAGCTGGACCAGCGGGTTTATCAGCCTCTATCTCTTGTGCTCGTTTTGGACTAAATGTACTT
GTTATTGATGAATTTATGAA

Product: putative sarcosine oxidase, alpha subunit

Products: glycine; formaldehyde; H2O2

Alternate protein names: Sarcosine Oxidase Subunit Alpha; Proline Dehydrogenase Alpha Subunit; FAD-Dependent Pyridine Nucleotide-Disulfide Oxidoreductase; FAD-Dependent Pyridine Nucleotide-Disulphide Oxidoreductase; D-Nopaline Dehydrogenase; SoxA Protein; Dye-Linked L-Proline Dehydrogenase; Dye-L-ProDH Alpha

Number of amino acids: Translated: 110; Mature: 109

Protein sequence:

>110_residues
MSRITHHPILGSLNNSNHITFQFNGQQYEAYEHETIAAALLANGIRTLRVHEDNGTPRGIYCNIGHCSECRVTVNNQANV
RACLTVVENGMVVDSGKQHPNIVREMVKKR

Sequences:

>Translated_110_residues
MSRITHHPILGSLNNSNHITFQFNGQQYEAYEHETIAAALLANGIRTLRVHEDNGTPRGIYCNIGHCSECRVTVNNQANV
RACLTVVENGMVVDSGKQHPNIVREMVKKR
>Mature_109_residues
SRITHHPILGSLNNSNHITFQFNGQQYEAYEHETIAAALLANGIRTLRVHEDNGTPRGIYCNIGHCSECRVTVNNQANVR
ACLTVVENGMVVDSGKQHPNIVREMVKKR

Specific function: Unknown

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.5.3.1

Molecular weight: Translated: 12310; Mature: 12179

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRITHHPILGSLNNSNHITFQFNGQQYEAYEHETIAAALLANGIRTLRVHEDNGTPRGI
CCCCCCCCEEECCCCCCEEEEEECCCEEECCCHHHHHHHHHHCCEEEEEEECCCCCCCEE
YCNIGHCSECRVTVNNQANVRACLTVVENGMVVDSGKQHPNIVREMVKKR
EEECCCCCCEEEEECCCCCHHHEEEHHHCCEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SRITHHPILGSLNNSNHITFQFNGQQYEAYEHETIAAALLANGIRTLRVHEDNGTPRGI
CCCCCCCEEECCCCCCEEEEEECCCEEECCCHHHHHHHHHHCCEEEEEEECCCCCCCEE
YCNIGHCSECRVTVNNQANVRACLTVVENGMVVDSGKQHPNIVREMVKKR
EEECCCCCCEEEEECCCCCHHHEEEHHHCCEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sarcosine; H2O; O2

Specific reaction: sarcosine + H2O + O2 = glycine + formaldehyde + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA