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The map label for this gene is ppsA [H]

Identifier: 227814087

GI number: 227814087

Start: 1370292

End: 1372898

Strand: Direct

Name: ppsA [H]

Synonym: BAMEG_1494

Alternate gene names: 227814087

Gene position: 1370292-1372898 (Clockwise)

Preceding gene: 227814084

Following gene: 227814089

Centisome position: 26.2

GC content: 38.55

Gene sequence:

>2607_bases
ATGAGTTCTTTCGTTCTCGATTTTCAGGAAATAGAAAAAACGCAGCTTTCACTCGTTGGTGGAAAAGGTTTGAATTTAGG
GGCGTTATCCAACATGCAAGGGATACAAGTGCCAGAAGGTTTTTGTGTTACAACGGCAGGATATGAAAAGGCCATCGAGC
AAAATGAGGAGCTTCAAGCTTTGTTGCAACAGCTAACAAAGTTGAAACGTGAAGAGCGAGTTCGAATTGGTGAAATCAGC
AAGAAGATTAGAGAAAACATTATGGCGGTACAAATCCCTTCTGATGTAGTGGAAGCAGTAGCTCATTATCTCTCACGCTT
TGGAAATGAACATGCGTATGCCGTACGCTCTAGTGCGACGGCTGAAGATTTACCGTATGCCTCGTTTGCTGGTCAACAAG
ATACGTATTTAAATATTATCGGAGAAAACGCAATTTTACAGCATGTAAGAAAGTGCTGGGCTTCTTTATTTACAGATCGT
GCAGTTATGTACCGCATGCAAAATGGTTTTGAACATAATCAAGTTTCTATATGTGTTGTCGTTCAAAAAATGGTTTTTCC
GCAGGCTTCAGGAATTTTATTTACCGCTGATCCGATTACTTCTAACCGGAAGGTGTTATCAATCGATGCCAGTTTTGGAC
TTGGCGAGGCATTAGTATCGGGACTCGTCTCTGCCGATAATTATAAAGTAAAAGAAGGCGAAATCATCGATAAGATGATT
GCAACTAAAAAATTAGCTATTTATGCTTTAAAAGAGGGCGGAACTGAGACGAAACAGAATGATCCGGTTCAGCAAAAAAT
TCAAACACTATCTGACCAACAAATATTACAACTAGTACAGATTGGAAGGCAGATTGAAGCTTATTTTGGCTGTCCGCAAG
ATATTGAATGGTGTTTAGTCGATCACACATTTTATATTGTCCAAAGTCGTCCGATAACGACTTTATATCCAATTCCGGAA
GAAAATGATGAGGGAAATCATGTGTATATATCAGTTGGTCATCAACAAATGATGACCGATGCGATGAAACCACTAGGGTT
ATCTTTTTTCTTGTTAACGACTAATGCTCCTATGCGTAAAGCGGGAGGCAGGCTGTTTGTAGATGCTACGCAACAATTAG
TTTTACCTGCTAGCAGAGATTATTTAATAAACACTTTAGGGAAATCGGATCCACTTGTAAGAGATGCATTGACGACTATA
ATCGAGCGAGATGATTTTATTACATTATTACCGGATGAAGAAAAAGAAAAGAGTGCTAGCAAAAGTAAGCCACCAGTAAG
TTCGCAACCAGAAATCGAAAATGATCCAGCAATCGTTACGGAATTAATAAGGAATAGTGAAGCATCACTTGAAGAGTTAA
AACAAAATATGCAACTGAAATCAGGAGTGGATGTATTTGATTTTATTTTGGAAGACATCCAGCAATTAAAGAAAGTATTA
TTTAATCCGCAAAGTATAGCTGTCATCATGGCAGGTATGAATGCTTCAACATGGATTAATGAAAAAATGGAGCAGTGGCT
AGGTGAAAAGAATGCAGCAGACATGCTTTCTCAATCGGTACAAAATAATATTACATCAGAAATGGGTTTAGCATTAATGG
ATGTTGCTGATGCAATTCGTCCGTATCCAGAAGTTATTACGTATTTACAACATGTAGAAGATGATAGCTTTTTAGATGAA
CTTGTTCAGTATAAAGGCGGAGAAAAAGCTCGAGATACAATCGTTGCTTTTCTAAATAAATACGGAATGAGATGTAGCGG
CGAAATCGACATTACGAAAACGCGCTGGAGTGAGAAGCCAACAACCATTATCCCAATGATATTAAATAATATAAGAGACT
TTGAAGCGGGTGCTAGTAAGCAGAAGTTTGAAAAAGGACTGCAGGAAGCTTTGAATAAAGAAGAAGAGTTATTAGACCGA
TTGCAACATTTGCCGAAGGGTAAACAAAAAGTAGAAGAAACGAAGCGAATGATTCGTAATATCCGTAATTTTATTGGCTA
TCGTGAATATCCAAAATACGGCATGATTAATCGTTACTTCATATATAAGCAGGCGTTATTGAAAGAAGCTGAGCAACTTG
TACAAAGCGGTGTCATTTGTGAGGTCGATGATATATACTATCTAACTTTTGAAGAACTTCATGAAGTTGTTCGCACGAAT
AAACTAGATTACGAGCTTATCCAAAAACAAAAAAATGATTATAAATTGTATGAAAAACTAACGCCTCCCCGCGTTATGAC
GTCTGATGGAGAAATCATTACAGGTAAATATAAACGAGAAAATCTCCCAGCTGACGCGATTGTAGGCCTGCCTGTTTCTT
CCGGAGTAGTTGAGGGGAGAGCCCGTGTTATTTTAAACATGGAAGAAGCGAATTTAGAAGAGGGAGATATATTAGTTACT
GCTTTTACTGATCCTGGCTGGACACCGTTGTTTGTTTCTATAAAAGGGTTAGTGACCGAAGTTGGTGGGCTCATGACACA
CGGGGCAGTGATTGCACGTGAATATGGATTACCAGCAGTTGTTGGAGTAGAGAATGCTACGAAACGAATCAAAGATGGGC
AACGAATTCGGGTACATGGAACAGAAGGGTATATTGAAATGTTGTAA

Upstream 100 bases:

>100_bases
AACTTAATTATACCACGTAAATAAAATTCAAAATAGACTGTTTCTTCTTATGTACTACTGCTACAATCAAGAACACATGC
ATAGTAAGGAGAGATGTTAC

Downstream 100 bases:

>100_bases
TGAAACAAAGGATCATCCGCTATTATAGTGGATGATCCTTTGTTTCATATATACTTGACTTATTTCTTTAAACCAGCTGC
ATAAAAAGGTCCATGAACTG

Product: phosphoenolpyruvate synthase

Products: AMP; phosphoenolpyruvate; phosphate

Alternate protein names: Putative PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 868; Mature: 867

Protein sequence:

>868_residues
MSSFVLDFQEIEKTQLSLVGGKGLNLGALSNMQGIQVPEGFCVTTAGYEKAIEQNEELQALLQQLTKLKREERVRIGEIS
KKIRENIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGENAILQHVRKCWASLFTDR
AVMYRMQNGFEHNQVSICVVVQKMVFPQASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSADNYKVKEGEIIDKMI
ATKKLAIYALKEGGTETKQNDPVQQKIQTLSDQQILQLVQIGRQIEAYFGCPQDIEWCLVDHTFYIVQSRPITTLYPIPE
ENDEGNHVYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRKAGGRLFVDATQQLVLPASRDYLINTLGKSDPLVRDALTTI
IERDDFITLLPDEEKEKSASKSKPPVSSQPEIENDPAIVTELIRNSEASLEELKQNMQLKSGVDVFDFILEDIQQLKKVL
FNPQSIAVIMAGMNASTWINEKMEQWLGEKNAADMLSQSVQNNITSEMGLALMDVADAIRPYPEVITYLQHVEDDSFLDE
LVQYKGGEKARDTIVAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEAGASKQKFEKGLQEALNKEEELLDR
LQHLPKGKQKVEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVICEVDDIYYLTFEELHEVVRTN
KLDYELIQKQKNDYKLYEKLTPPRVMTSDGEIITGKYKRENLPADAIVGLPVSSGVVEGRARVILNMEEANLEEGDILVT
AFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKRIKDGQRIRVHGTEGYIEML

Sequences:

>Translated_868_residues
MSSFVLDFQEIEKTQLSLVGGKGLNLGALSNMQGIQVPEGFCVTTAGYEKAIEQNEELQALLQQLTKLKREERVRIGEIS
KKIRENIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGENAILQHVRKCWASLFTDR
AVMYRMQNGFEHNQVSICVVVQKMVFPQASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSADNYKVKEGEIIDKMI
ATKKLAIYALKEGGTETKQNDPVQQKIQTLSDQQILQLVQIGRQIEAYFGCPQDIEWCLVDHTFYIVQSRPITTLYPIPE
ENDEGNHVYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRKAGGRLFVDATQQLVLPASRDYLINTLGKSDPLVRDALTTI
IERDDFITLLPDEEKEKSASKSKPPVSSQPEIENDPAIVTELIRNSEASLEELKQNMQLKSGVDVFDFILEDIQQLKKVL
FNPQSIAVIMAGMNASTWINEKMEQWLGEKNAADMLSQSVQNNITSEMGLALMDVADAIRPYPEVITYLQHVEDDSFLDE
LVQYKGGEKARDTIVAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEAGASKQKFEKGLQEALNKEEELLDR
LQHLPKGKQKVEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVICEVDDIYYLTFEELHEVVRTN
KLDYELIQKQKNDYKLYEKLTPPRVMTSDGEIITGKYKRENLPADAIVGLPVSSGVVEGRARVILNMEEANLEEGDILVT
AFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKRIKDGQRIRVHGTEGYIEML
>Mature_867_residues
SSFVLDFQEIEKTQLSLVGGKGLNLGALSNMQGIQVPEGFCVTTAGYEKAIEQNEELQALLQQLTKLKREERVRIGEISK
KIRENIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGENAILQHVRKCWASLFTDRA
VMYRMQNGFEHNQVSICVVVQKMVFPQASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSADNYKVKEGEIIDKMIA
TKKLAIYALKEGGTETKQNDPVQQKIQTLSDQQILQLVQIGRQIEAYFGCPQDIEWCLVDHTFYIVQSRPITTLYPIPEE
NDEGNHVYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRKAGGRLFVDATQQLVLPASRDYLINTLGKSDPLVRDALTTII
ERDDFITLLPDEEKEKSASKSKPPVSSQPEIENDPAIVTELIRNSEASLEELKQNMQLKSGVDVFDFILEDIQQLKKVLF
NPQSIAVIMAGMNASTWINEKMEQWLGEKNAADMLSQSVQNNITSEMGLALMDVADAIRPYPEVITYLQHVEDDSFLDEL
VQYKGGEKARDTIVAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEAGASKQKFEKGLQEALNKEEELLDRL
QHLPKGKQKVEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVICEVDDIYYLTFEELHEVVRTNK
LDYELIQKQKNDYKLYEKLTPPRVMTSDGEIITGKYKRENLPADAIVGLPVSSGVVEGRARVILNMEEANLEEGDILVTA
FTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKRIKDGQRIRVHGTEGYIEML

Specific function: Might catalyze the phosphorylation of pyruvate to phosphoenolpyruvate (Potential) [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=335, Percent_Identity=34.9253731343284, Blast_Score=176, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17564524, Length=324, Percent_Identity=29.6296296296296, Blast_Score=125, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR002192 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]

EC number: 2.7.9.2

Molecular weight: Translated: 97618; Mature: 97487

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFVLDFQEIEKTQLSLVGGKGLNLGALSNMQGIQVPEGFCVTTAGYEKAIEQNEELQA
CCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHCCHHHHH
LLQQLTKLKREERVRIGEISKKIRENIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSAT
HHHHHHHHHHHHCCHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCC
AEDLPYASFAGQQDTYLNIIGENAILQHVRKCWASLFTDRAVMYRMQNGFEHNQVSICVV
CCCCCCHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHH
VQKMVFPQASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSADNYKVKEGEIIDKMI
HHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEECCCHHHHHHH
ATKKLAIYALKEGGTETKQNDPVQQKIQTLSDQQILQLVQIGRQIEAYFGCPQDIEWCLV
HHHHHEEEEEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
DHTFYIVQSRPITTLYPIPEENDEGNHVYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRK
EEEEEEEECCCCEEEECCCCCCCCCCEEEEEECHHHHHHHHHHHCCCEEEEEECCCCHHH
AGGRLFVDATQQLVLPASRDYLINTLGKSDPLVRDALTTIIERDDFITLLPDEEKEKSAS
CCCEEEEECHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHC
KSKPPVSSQPEIENDPAIVTELIRNSEASLEELKQNMQLKSGVDVFDFILEDIQQLKKVL
CCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FNPQSIAVIMAGMNASTWINEKMEQWLGEKNAADMLSQSVQNNITSEMGLALMDVADAIR
CCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PYPEVITYLQHVEDDSFLDELVQYKGGEKARDTIVAFLNKYGMRCSGEIDITKTRWSEKP
CHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
TTIIPMILNNIRDFEAGASKQKFEKGLQEALNKEEELLDRLQHLPKGKQKVEETKRMIRN
CEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVICEVDDIYYLTFEELHEVVRTN
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEEHHHHHHHHHCC
KLDYELIQKQKNDYKLYEKLTPPRVMTSDGEIITGKYKRENLPADAIVGLPVSSGVVEGR
CCCHHHHHHCCCCHHHHHHCCCCCEEECCCCEEECCCCCCCCCCCCEEECCCCCCCCCCC
ARVILNMEEANLEEGDILVTAFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAV
EEEEEEEHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCEE
VGVENATKRIKDGQRIRVHGTEGYIEML
EECCHHHHHCCCCCEEEEECCCCCEECC
>Mature Secondary Structure 
SSFVLDFQEIEKTQLSLVGGKGLNLGALSNMQGIQVPEGFCVTTAGYEKAIEQNEELQA
CCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHCCHHHHH
LLQQLTKLKREERVRIGEISKKIRENIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSAT
HHHHHHHHHHHHCCHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCC
AEDLPYASFAGQQDTYLNIIGENAILQHVRKCWASLFTDRAVMYRMQNGFEHNQVSICVV
CCCCCCHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHH
VQKMVFPQASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSADNYKVKEGEIIDKMI
HHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEECCCHHHHHHH
ATKKLAIYALKEGGTETKQNDPVQQKIQTLSDQQILQLVQIGRQIEAYFGCPQDIEWCLV
HHHHHEEEEEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
DHTFYIVQSRPITTLYPIPEENDEGNHVYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRK
EEEEEEEECCCCEEEECCCCCCCCCCEEEEEECHHHHHHHHHHHCCCEEEEEECCCCHHH
AGGRLFVDATQQLVLPASRDYLINTLGKSDPLVRDALTTIIERDDFITLLPDEEKEKSAS
CCCEEEEECHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHC
KSKPPVSSQPEIENDPAIVTELIRNSEASLEELKQNMQLKSGVDVFDFILEDIQQLKKVL
CCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FNPQSIAVIMAGMNASTWINEKMEQWLGEKNAADMLSQSVQNNITSEMGLALMDVADAIR
CCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PYPEVITYLQHVEDDSFLDELVQYKGGEKARDTIVAFLNKYGMRCSGEIDITKTRWSEKP
CHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
TTIIPMILNNIRDFEAGASKQKFEKGLQEALNKEEELLDRLQHLPKGKQKVEETKRMIRN
CEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVICEVDDIYYLTFEELHEVVRTN
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEEHHHHHHHHHCC
KLDYELIQKQKNDYKLYEKLTPPRVMTSDGEIITGKYKRENLPADAIVGLPVSSGVVEGR
CCCHHHHHHCCCCHHHHHHCCCCCEEECCCCEEECCCCCCCCCCCCEEECCCCCCCCCCC
ARVILNMEEANLEEGDILVTAFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAV
EEEEEEEHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCEE
VGVENATKRIKDGQRIRVHGTEGYIEML
EECCHHHHHCCCCCEEEEECCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10.5 {phosphate}} 0.028 {ATP}} 0.083 {pyruvate}} [C]

Substrates: ATP; pyruvate; H2O

Specific reaction: ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 7704256 [H]