Click here to switch to the map view.

The map label for this gene is ytsJ [H]

Identifier: 227814059

GI number: 227814059

Start: 1346270

End: 1347514

Strand: Direct

Name: ytsJ [H]

Synonym: BAMEG_1466

Alternate gene names: 227814059

Gene position: 1346270-1347514 (Clockwise)

Preceding gene: 227814058

Following gene: 227814060

Centisome position: 25.74

GC content: 35.9

Gene sequence:

>1245_bases
ATGTCACTTTTACGAGATGAAGCATTAAAAATGCATAAAGAAAAACAGGGGAAGATTGGTGTTTATTCTAAAGTTAAAGT
ACAAAATTCAACAGATTTAAGTCTCGCTTACTCACCAGGTGTTGCTGAGCCATGCTTAGAGATTTATAAAGATGAAAGCA
AAGTATATGATTATACAATGAAAGGGAATCTAGTTGGAGTTATATCTGATGGAACTGCAGTATTAGGTCTTGGGAATATT
GGGCCTAAAGCAGCTATGCCAGTGATGGAGGGAAAAGCTTTACTTTTCAAATCATTTGCTGAGATCGATGCTTTTCCTAT
TTGTTTAAACACTAATAATCCGGATAAGATTGTTGAGGTTGTTAAATTATTAGAACCAAATTTTGGTGGGATAAACTTAG
AAGATATTGCAGCACCACAATGCTTTGAAATTGAGGAGCGCCTAAGAAAATCTTGTGATATTCCTGTTTTCCATGATGAT
CAACACGGTACAGCTATTGTAACGATTGCTGGCCTAATTAATGCACTTAAGTTAACAAATAAAAAAATTGAAGATGTGCA
AATCGTTGTGAATGGTGCTGGTGCTGCTGGGATAGCGATTGTTAAGCTGTTACTTCATATGCATGTAAAAAATGTTATTT
TGTGTGACACAAAAGGGATAATATATGAACAGCGACCTATTGGAATGAATGCGCTTAAAGAAGAAATAGCTCGTATTACA
AATATAGAACAGAAGCGGGGAACATTAGCAGAGGCCCTTATTGATGCTGATGTATTTATTGGAGTATCAGCTGCAGATGT
AGTTGATGAGGATATGATTCGTTCTATGAATCATAATCCTATTATTTTTGCCTTGGCTAACCCTACACCTGAAATTATGC
CTAATAAAGCAAAAATAGCAGGAGCACTAGTAGTTGGTACAGGACGTTCTGATTTTCCTAATCAAGTAAATAATGTACTA
GCTTTCCCCGGAGTTTTTCGTGGAGCTTTAGATGTACAAGCAAAAGAAATTAATGAAGAAATGAAGCTAGCAGCTGTTTA
TGCAATAGCTGAGTTAATTTCAGAAGAAGAGCTTCATGTCAACTATGTCATTCCAAATCCGTTTGATCCTAGGGTGGTGC
CACATGTAGCATATGCTGTCGCACGTGCTGCAGTGGAAACCGGTGTTTCAAGAAAAAATATCAGCATGTCTGAAATTAAA
AACAATCTATTCAGGTTAACAAAAGAGGTAAATATATTAAAATAA

Upstream 100 bases:

>100_bases
ATTAGAAGTCTTACTTATATCATACAATATCAAGTTTAATATAGAGTTAATAAAATATTGATTTAATTAATTCTTAACTT
AATTATAGGAGCGAAATTAT

Downstream 100 bases:

>100_bases
ATATTTTTCTTAAGAAAATATTATGTAGTAAAGGTTATCTACTTAGTTCTTAGCGTATAAAAAGTTAAATTATGTATATC
TCCCTTTCTGATATTTGTAA

Product: putative malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MSLLRDEALKMHKEKQGKIGVYSKVKVQNSTDLSLAYSPGVAEPCLEIYKDESKVYDYTMKGNLVGVISDGTAVLGLGNI
GPKAAMPVMEGKALLFKSFAEIDAFPICLNTNNPDKIVEVVKLLEPNFGGINLEDIAAPQCFEIEERLRKSCDIPVFHDD
QHGTAIVTIAGLINALKLTNKKIEDVQIVVNGAGAAGIAIVKLLLHMHVKNVILCDTKGIIYEQRPIGMNALKEEIARIT
NIEQKRGTLAEALIDADVFIGVSAADVVDEDMIRSMNHNPIIFALANPTPEIMPNKAKIAGALVVGTGRSDFPNQVNNVL
AFPGVFRGALDVQAKEINEEMKLAAVYAIAELISEEELHVNYVIPNPFDPRVVPHVAYAVARAAVETGVSRKNISMSEIK
NNLFRLTKEVNILK

Sequences:

>Translated_414_residues
MSLLRDEALKMHKEKQGKIGVYSKVKVQNSTDLSLAYSPGVAEPCLEIYKDESKVYDYTMKGNLVGVISDGTAVLGLGNI
GPKAAMPVMEGKALLFKSFAEIDAFPICLNTNNPDKIVEVVKLLEPNFGGINLEDIAAPQCFEIEERLRKSCDIPVFHDD
QHGTAIVTIAGLINALKLTNKKIEDVQIVVNGAGAAGIAIVKLLLHMHVKNVILCDTKGIIYEQRPIGMNALKEEIARIT
NIEQKRGTLAEALIDADVFIGVSAADVVDEDMIRSMNHNPIIFALANPTPEIMPNKAKIAGALVVGTGRSDFPNQVNNVL
AFPGVFRGALDVQAKEINEEMKLAAVYAIAELISEEELHVNYVIPNPFDPRVVPHVAYAVARAAVETGVSRKNISMSEIK
NNLFRLTKEVNILK
>Mature_413_residues
SLLRDEALKMHKEKQGKIGVYSKVKVQNSTDLSLAYSPGVAEPCLEIYKDESKVYDYTMKGNLVGVISDGTAVLGLGNIG
PKAAMPVMEGKALLFKSFAEIDAFPICLNTNNPDKIVEVVKLLEPNFGGINLEDIAAPQCFEIEERLRKSCDIPVFHDDQ
HGTAIVTIAGLINALKLTNKKIEDVQIVVNGAGAAGIAIVKLLLHMHVKNVILCDTKGIIYEQRPIGMNALKEEIARITN
IEQKRGTLAEALIDADVFIGVSAADVVDEDMIRSMNHNPIIFALANPTPEIMPNKAKIAGALVVGTGRSDFPNQVNNVLA
FPGVFRGALDVQAKEINEEMKLAAVYAIAELISEEELHVNYVIPNPFDPRVVPHVAYAVARAAVETGVSRKNISMSEIKN
NLFRLTKEVNILK

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=362, Percent_Identity=26.7955801104972, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI62420882, Length=363, Percent_Identity=27.5482093663912, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI62420880, Length=363, Percent_Identity=27.5482093663912, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI239049447, Length=363, Percent_Identity=27.5482093663912, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI4505145, Length=361, Percent_Identity=27.1468144044321, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI270265879, Length=320, Percent_Identity=27.8125, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1788806, Length=429, Percent_Identity=50.5827505827506, Blast_Score=400, Evalue=1e-112,
Organism=Escherichia coli, GI87081919, Length=456, Percent_Identity=25.8771929824561, Blast_Score=88, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17537199, Length=423, Percent_Identity=27.1867612293144, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322823, Length=347, Percent_Identity=28.2420749279539, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24646388, Length=359, Percent_Identity=28.9693593314763, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24646386, Length=359, Percent_Identity=28.9693593314763, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI78707242, Length=408, Percent_Identity=26.9607843137255, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI78707238, Length=408, Percent_Identity=26.9607843137255, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI78707240, Length=361, Percent_Identity=27.7008310249307, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI21356279, Length=451, Percent_Identity=25.4988913525499, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281362672, Length=451, Percent_Identity=25.4988913525499, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI281362674, Length=451, Percent_Identity=25.4988913525499, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI78707232, Length=389, Percent_Identity=26.2210796915167, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281363505, Length=358, Percent_Identity=26.536312849162, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI78707236, Length=358, Percent_Identity=26.536312849162, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281363503, Length=358, Percent_Identity=26.536312849162, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 45088; Mature: 44957

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLRDEALKMHKEKQGKIGVYSKVKVQNSTDLSLAYSPGVAEPCLEIYKDESKVYDYTM
CCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHCCCCCEEEEEE
KGNLVGVISDGTAVLGLGNIGPKAAMPVMEGKALLFKSFAEIDAFPICLNTNNPDKIVEV
CCCEEEEEECCCEEEEECCCCCHHCCCHHCCHHHHHHHHHHCCCEEEEEECCCHHHHHHH
VKLLEPNFGGINLEDIAAPQCFEIEERLRKSCDIPVFHDDQHGTAIVTIAGLINALKLTN
HHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHHCC
KKIEDVQIVVNGAGAAGIAIVKLLLHMHVKNVILCDTKGIIYEQRPIGMNALKEEIARIT
CCCCEEEEEEECCCHHHHHHHHHHHHHHHCCEEEECCCCCEEECCCCCHHHHHHHHHHHH
NIEQKRGTLAEALIDADVFIGVSAADVVDEDMIRSMNHNPIIFALANPTPEIMPNKAKIA
CCHHHHHHHHHHHHCCHHEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHEE
GALVVGTGRSDFPNQVNNVLAFPGVFRGALDVQAKEINEEMKLAAVYAIAELISEEELHV
EEEEEECCCCCCCHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
NYVIPNPFDPRVVPHVAYAVARAAVETGVSRKNISMSEIKNNLFRLTKEVNILK
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SLLRDEALKMHKEKQGKIGVYSKVKVQNSTDLSLAYSPGVAEPCLEIYKDESKVYDYTM
CCHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHCCCCCEEEEEE
KGNLVGVISDGTAVLGLGNIGPKAAMPVMEGKALLFKSFAEIDAFPICLNTNNPDKIVEV
CCCEEEEEECCCEEEEECCCCCHHCCCHHCCHHHHHHHHHHCCCEEEEEECCCHHHHHHH
VKLLEPNFGGINLEDIAAPQCFEIEERLRKSCDIPVFHDDQHGTAIVTIAGLINALKLTN
HHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHHCC
KKIEDVQIVVNGAGAAGIAIVKLLLHMHVKNVILCDTKGIIYEQRPIGMNALKEEIARIT
CCCCEEEEEEECCCHHHHHHHHHHHHHHHCCEEEECCCCCEEECCCCCHHHHHHHHHHHH
NIEQKRGTLAEALIDADVFIGVSAADVVDEDMIRSMNHNPIIFALANPTPEIMPNKAKIA
CCHHHHHHHHHHHHCCHHEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHEE
GALVVGTGRSDFPNQVNNVLAFPGVFRGALDVQAKEINEEMKLAAVYAIAELISEEELHV
EEEEEECCCCCCCHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
NYVIPNPFDPRVVPHVAYAVARAAVETGVSRKNISMSEIKNNLFRLTKEVNILK
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]