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The map label for this gene is pykA1 [H]

Identifier: 227813838

GI number: 227813838

Start: 1125488

End: 1126546

Strand: Direct

Name: pykA1 [H]

Synonym: BAMEG_1243

Alternate gene names: 227813838

Gene position: 1125488-1126546 (Clockwise)

Preceding gene: 227813837

Following gene: 227813841

Centisome position: 21.52

GC content: 35.69

Gene sequence:

>1059_bases
ATGACAATCGATCGAGTTTGTACAATTGGGCCAGCAAGTAATAATAAAGAAACGTTAGCGAAGTTAATAAACAATGGTAT
GAAAATTGTTCGTCTAAATTTATCACATGGTACGCATGAAAGTCATAAAGATATTATTCGTTTAGTGAAATCGTTAGATG
ATTCTATTAAAATTTTAGGCGATGTACAAGGTCCTAAAATAAGGTTAGGTGAAATAAAAGGAGAACAAATTACACTTCAG
GCGGGAGATTCTTTTATGTTACGTACGCAACCAGTTACAGGGAGTAGTACGGAAGCAAGTGTTGATTATGAAGGAATCGC
GAATGATGTGAAAGTTGGAAGTAGAATTTTAATGAATGATGGCGAAGTTGAGTTAATTGTTGAGAAGGTAAGTACGGATA
AAATAGAAACAAAGGTCAAAACAGGTGGTAATATCTCATCACATAAAGGAGTGAACTTACCAGGGGCAATCGTTAGTTTA
CCAGCTATTACAGAGAAAGATAAAAAAGATATTCAGTTTCTTTTAGAAGAGGATGTTGATTTTATTGCGTGTTCTTTCGT
AAGAAAACCTAGTCATATAAAAGAAATACGAGATTTTATACAACAGTATAAAGAAACGTCACCTAATTTAATTGCAAAAA
TAGAAACGATGGAAGCAATCGAGAATTTTCAAGATATATGTAAAGAAGCAGACGGAATTATGATTGCAAGGGGCGATTTA
GGAGTTGAGTTGCCGTATCAATTTATTCCGCTCTTGCAGAAAATGATGATTCAGGAATGTAATCGAACGAATACATATGT
TATTACAGCGACACAAATGCTTCAATCTATGGTAGATCATTCGATTCCAACAAGGGCTGAGGTAACTGATGTGTTTCAAG
CTGTACTGGATGGAACGAATGCTGTTATGCTTTCTGCTGAAAGTGCTTCAGGAGAGCATCCGGTTGAAAGTGTGAGTACA
CTACGTCTCGTTTCTGAATTTGCTGAGCATGTAAAAAAAGACGGTCCTTTTGTGATGAAAGATGTACTGGAATTGCTGCA
TAAATCTTTGGATGAGTGA

Upstream 100 bases:

>100_bases
TTTCGTAGTTTCAAAAGCCTACTTGTGTGAGTAGGCTTTTTTTACGTTGAGAGTGTAATTAAAGTTTATGGGAATCCTAA
TGAAAAAGGATGGTGGAAAT

Downstream 100 bases:

>100_bases
AAAATAAAAAGAGGGCGGATATGTATCCGTCCTCGTGTGTTTCATTCTATTCTTGCTCTTGCTCATCCAAAATCTTTTCA
AACGCAGCAGATACTTTTTC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 2 [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MTIDRVCTIGPASNNKETLAKLINNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKILGDVQGPKIRLGEIKGEQITLQ
AGDSFMLRTQPVTGSSTEASVDYEGIANDVKVGSRILMNDGEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVSL
PAITEKDKKDIQFLLEEDVDFIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDICKEADGIMIARGDL
GVELPYQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTNAVMLSAESASGEHPVESVST
LRLVSEFAEHVKKDGPFVMKDVLELLHKSLDE

Sequences:

>Translated_352_residues
MTIDRVCTIGPASNNKETLAKLINNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKILGDVQGPKIRLGEIKGEQITLQ
AGDSFMLRTQPVTGSSTEASVDYEGIANDVKVGSRILMNDGEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVSL
PAITEKDKKDIQFLLEEDVDFIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDICKEADGIMIARGDL
GVELPYQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTNAVMLSAESASGEHPVESVST
LRLVSEFAEHVKKDGPFVMKDVLELLHKSLDE
>Mature_351_residues
TIDRVCTIGPASNNKETLAKLINNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKILGDVQGPKIRLGEIKGEQITLQA
GDSFMLRTQPVTGSSTEASVDYEGIANDVKVGSRILMNDGEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVSLP
AITEKDKKDIQFLLEEDVDFIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDICKEADGIMIARGDLG
VELPYQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTNAVMLSAESASGEHPVESVSTL
RLVSEFAEHVKKDGPFVMKDVLELLHKSLDE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=340, Percent_Identity=42.6470588235294, Blast_Score=275, Evalue=3e-74,
Organism=Homo sapiens, GI33286420, Length=340, Percent_Identity=42.6470588235294, Blast_Score=275, Evalue=3e-74,
Organism=Homo sapiens, GI33286418, Length=340, Percent_Identity=42.6470588235294, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI10835121, Length=340, Percent_Identity=40.8823529411765, Blast_Score=254, Evalue=8e-68,
Organism=Homo sapiens, GI32967597, Length=340, Percent_Identity=40.8823529411765, Blast_Score=254, Evalue=8e-68,
Organism=Homo sapiens, GI310128732, Length=283, Percent_Identity=40.9893992932862, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI310128730, Length=283, Percent_Identity=40.9893992932862, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI310128736, Length=231, Percent_Identity=38.5281385281385, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI310128734, Length=231, Percent_Identity=38.5281385281385, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=36.7441860465116, Blast_Score=139, Evalue=3e-33,
Organism=Escherichia coli, GI1787965, Length=332, Percent_Identity=43.6746987951807, Blast_Score=285, Evalue=2e-78,
Organism=Escherichia coli, GI1788160, Length=335, Percent_Identity=40.5970149253731, Blast_Score=230, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI71984413, Length=336, Percent_Identity=46.4285714285714, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI71984406, Length=336, Percent_Identity=46.4285714285714, Blast_Score=279, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17506829, Length=336, Percent_Identity=46.4285714285714, Blast_Score=279, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17506831, Length=336, Percent_Identity=46.4285714285714, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17544584, Length=350, Percent_Identity=41.4285714285714, Blast_Score=265, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6319279, Length=336, Percent_Identity=38.0952380952381, Blast_Score=220, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6324923, Length=334, Percent_Identity=35.9281437125749, Blast_Score=201, Evalue=1e-52,
Organism=Drosophila melanogaster, GI28571814, Length=340, Percent_Identity=43.8235294117647, Blast_Score=271, Evalue=5e-73,
Organism=Drosophila melanogaster, GI24648964, Length=340, Percent_Identity=43.8235294117647, Blast_Score=271, Evalue=6e-73,
Organism=Drosophila melanogaster, GI24648966, Length=322, Percent_Identity=39.7515527950311, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24581235, Length=339, Percent_Identity=34.5132743362832, Blast_Score=201, Evalue=7e-52,
Organism=Drosophila melanogaster, GI24646914, Length=182, Percent_Identity=39.5604395604396, Blast_Score=139, Evalue=3e-33,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 38860; Mature: 38729

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDRVCTIGPASNNKETLAKLINNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKILG
CCCCCEEECCCCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCHHHEEHC
DVQGPKIRLGEIKGEQITLQAGDSFMLRTQPVTGSSTEASVDYEGIANDVKVGSRILMND
CCCCCEEEEEECCCCEEEEEECCCEEEEECCCCCCCCCCCCCHHHCHHHHHHCCEEEECC
GEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVSLPAITEKDKKDIQFLLEEDVD
CCEEEEEEHHCCHHHHHHHCCCCCCCCCCCCCCCHHHEECCCCCCCCHHHHHHHHHHCCH
FIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDICKEADGIMIARGDL
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
GVELPYQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTN
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
AVMLSAESASGEHPVESVSTLRLVSEFAEHVKKDGPFVMKDVLELLHKSLDE
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIDRVCTIGPASNNKETLAKLINNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKILG
CCCCEEECCCCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCHHHEEHC
DVQGPKIRLGEIKGEQITLQAGDSFMLRTQPVTGSSTEASVDYEGIANDVKVGSRILMND
CCCCCEEEEEECCCCEEEEEECCCEEEEECCCCCCCCCCCCCHHHCHHHHHHCCEEEECC
GEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVSLPAITEKDKKDIQFLLEEDVD
CCEEEEEEHHCCHHHHHHHCCCCCCCCCCCCCCCHHHEECCCCCCCCHHHHHHHHHHCCH
FIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDICKEADGIMIARGDL
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
GVELPYQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTN
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
AVMLSAESASGEHPVESVSTLRLVSEFAEHVKKDGPFVMKDVLELLHKSLDE
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]