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The map label for this gene is cwlH [H]

Identifier: 227813494

GI number: 227813494

Start: 801333

End: 803084

Strand: Direct

Name: cwlH [H]

Synonym: BAMEG_0898

Alternate gene names: 227813494

Gene position: 801333-803084 (Clockwise)

Preceding gene: 227813493

Following gene: 227813495

Centisome position: 15.32

GC content: 36.42

Gene sequence:

>1752_bases
ATGATATGCGTGTTAAGTAGTATGCTGAAAAAAGGATTTTATAATGTTTTAGCTGCATCAATTGTATTTAGTATGGTAAC
GATTCCAAATTATTCCTATGCTAACGAACTTGACAAAACGGTTACAGTTTCACCTGATGAACAAGCTTTAAAAAGTATTG
AAAACCATATGAAAGATGAAGATGGTAGAGGAGAAGATAAAGGAGTAAGAAATGAAGTGCAAGGTGAATTTCTTGTACAT
ATAGTGAAAGAGGTACCTTTATACGATTCTAGTAATTTTCAAAAAGAGACAGGGGTTCGTATTTCGAATCAAGTCGTAAA
AGCTGAGAAGAGAAAAGGAAACGCTTACTATGTTCAAACTTCATCGGGAACTGGTTGGATACAAAATAGCGATGGGAATG
TAGAAGTTAAAGAAATTCACCCGTTATTAAGTGAGAAGTTAATTGTAAATGAAGAAACCTCTACATACTCTGAACCATTT
GCTTCATACAAAGAAGAAAATGTACTGGAGCCACAAACTATTCAAGCAATTGGACAGGCTGGAGAATGGTTCCAAGTAAA
AATAAATAATGAGATGAAATGGATTCATTCACCTTCAGCAAAATTTGAAGGAACAAAAGCTTCGCTTATACAAGGCGCAG
CACCAATACGTACAAAATATGCAGCAGCTATGTATGCTGCACCAATAGAAGAGAAGACAACGGATATTTATGGTGTACCT
TTAAAAGAAATGATTGTACCGAAAGGAAATGAAAATATTCGTCCGGGTTATGCGATGAACCCGAAGTATATTACGATTCA
TGAGACAGATAATTACAGTGTGGGGGCAAATGCTAGAAACCATGCGATATATTTATATAACCAAGCAACGGGAACGGAAG
ATCGTTCAGCGTCGTGGCATTTTACAGTAGATGATAAAGAGATTTATCAACACCTGCCATTAAACGAAAATGCTTGGCAT
GCAGGAGATGGGGCAGAAGGAACTGGAAATAGAGAATCAATTGCGATTGAAATTGCTGTAAACGAAGATGGTGATTATAA
TAAAGCCGTTGAAAATGCTCGTAAACTAGCAGCTTATTTAATGGGGGAATTAAATATTCCTTTAGAGAATGTAAAAAAAC
ATCAATTTTGGAGTGGGAAGATTTGTCCAGCCATTATGATTAAAAATAATGGCTGGGAACCATTCTTACAAGGAACAAAA
ATGTATTATGATGCGAACCATAAAGATGATATAACGGGTGGTTGGTACGAAGCAGCCATTCGTGAATTAGATAAGCGCGG
CATTATGGTTGGAGATGGGAAAGGTTCTTATTGGCCAGAGCGTCTTGTGACACGTGGTGAATTTGCGAACTTCATTTCAA
GATCATTGCAGTTACCTGAAGGATCATCGAATTTTAGTGACTTAAATGCAGCACATCCATCGCTAATAGATGGTATTAAT
CGTGCAGCAAGTGCCGGGATAATAAGTGGTAGAGGAAATGGGATATTTGCTCCAAATGATACGATTAAACGAGATGAAGT
TGTTATCATGATTGACCGTGCACTGCAATATAAAAAAATTAAAGGTAACTTAGTTCCACTGCCATTCTCGGATCAAGATT
TAGCATATGATAAACAAGCTGTACAACGTGTTTATGGATTAGGCATTGTAAAAGGAAATGAAAATAATGAATTTATGCCG
AAAGGTTCAGCAACGCGTGGAGAATCAGCAGCATTTATTAATAGAATGTTAGAAGTAATAGAAAGTAATTAA

Upstream 100 bases:

>100_bases
AATTGGAATGAGTCTGTTATATTGTAAATGGTTATGTTGTTTAATAGTTTAGTAGATAACCTATATTTTGGCTGAGGAGG
GGAATTTTAACACGGGTTTA

Downstream 100 bases:

>100_bases
TAAAAAAGCATTCGCTTTTGCGAGTGCTTTTTTATTTTTCAATATGTTACGGAAGGGAGTGGAGGTTTTTGAGTGGAATA
AGGAATGAAAGTGGAGGTGA

Product: surface-layer N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MICVLSSMLKKGFYNVLAASIVFSMVTIPNYSYANELDKTVTVSPDEQALKSIENHMKDEDGRGEDKGVRNEVQGEFLVH
IVKEVPLYDSSNFQKETGVRISNQVVKAEKRKGNAYYVQTSSGTGWIQNSDGNVEVKEIHPLLSEKLIVNEETSTYSEPF
ASYKEENVLEPQTIQAIGQAGEWFQVKINNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPIEEKTTDIYGVP
LKEMIVPKGNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEIYQHLPLNENAWH
AGDGAEGTGNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPLENVKKHQFWSGKICPAIMIKNNGWEPFLQGTK
MYYDANHKDDITGGWYEAAIRELDKRGIMVGDGKGSYWPERLVTRGEFANFISRSLQLPEGSSNFSDLNAAHPSLIDGIN
RAASAGIISGRGNGIFAPNDTIKRDEVVIMIDRALQYKKIKGNLVPLPFSDQDLAYDKQAVQRVYGLGIVKGNENNEFMP
KGSATRGESAAFINRMLEVIESN

Sequences:

>Translated_583_residues
MICVLSSMLKKGFYNVLAASIVFSMVTIPNYSYANELDKTVTVSPDEQALKSIENHMKDEDGRGEDKGVRNEVQGEFLVH
IVKEVPLYDSSNFQKETGVRISNQVVKAEKRKGNAYYVQTSSGTGWIQNSDGNVEVKEIHPLLSEKLIVNEETSTYSEPF
ASYKEENVLEPQTIQAIGQAGEWFQVKINNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPIEEKTTDIYGVP
LKEMIVPKGNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEIYQHLPLNENAWH
AGDGAEGTGNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPLENVKKHQFWSGKICPAIMIKNNGWEPFLQGTK
MYYDANHKDDITGGWYEAAIRELDKRGIMVGDGKGSYWPERLVTRGEFANFISRSLQLPEGSSNFSDLNAAHPSLIDGIN
RAASAGIISGRGNGIFAPNDTIKRDEVVIMIDRALQYKKIKGNLVPLPFSDQDLAYDKQAVQRVYGLGIVKGNENNEFMP
KGSATRGESAAFINRMLEVIESN
>Mature_583_residues
MICVLSSMLKKGFYNVLAASIVFSMVTIPNYSYANELDKTVTVSPDEQALKSIENHMKDEDGRGEDKGVRNEVQGEFLVH
IVKEVPLYDSSNFQKETGVRISNQVVKAEKRKGNAYYVQTSSGTGWIQNSDGNVEVKEIHPLLSEKLIVNEETSTYSEPF
ASYKEENVLEPQTIQAIGQAGEWFQVKINNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPIEEKTTDIYGVP
LKEMIVPKGNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEIYQHLPLNENAWH
AGDGAEGTGNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPLENVKKHQFWSGKICPAIMIKNNGWEPFLQGTK
MYYDANHKDDITGGWYEAAIRELDKRGIMVGDGKGSYWPERLVTRGEFANFISRSLQLPEGSSNFSDLNAAHPSLIDGIN
RAASAGIISGRGNGIFAPNDTIKRDEVVIMIDRALQYKKIKGNLVPLPFSDQDLAYDKQAVQRVYGLGIVKGNENNEFMP
KGSATRGESAAFINRMLEVIESN

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 64893; Mature: 64893

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MICVLSSMLKKGFYNVLAASIVFSMVTIPNYSYANELDKTVTVSPDEQALKSIENHMKDE
CEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCHHHHHHHHHHHHCCC
DGRGEDKGVRNEVQGEFLVHIVKEVPLYDSSNFQKETGVRISNQVVKAEKRKGNAYYVQT
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCEEEEEE
SSGTGWIQNSDGNVEVKEIHPLLSEKLIVNEETSTYSEPFASYKEENVLEPQTIQAIGQA
CCCCCEEECCCCCEEHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHCCC
GEWFQVKINNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPIEEKTTDIYGVP
CCEEEEEECCCEEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEECCC
LKEMIVPKGNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQATGTEDRSASWH
HHHHCCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCEEEEEECCCCCCCCCCEEE
FTVDDKEIYQHLPLNENAWHAGDGAEGTGNRESIAIEIAVNEDGDYNKAVENARKLAAYL
EEECHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHH
MGELNIPLENVKKHQFWSGKICPAIMIKNNGWEPFLQGTKMYYDANHKDDITGGWYEAAI
HHCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCHHHCCCEEEECCCCCCCCCCHHHHHHH
RELDKRGIMVGDGKGSYWPERLVTRGEFANFISRSLQLPEGSSNFSDLNAAHPSLIDGIN
HHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
RAASAGIISGRGNGIFAPNDTIKRDEVVIMIDRALQYKKIKGNLVPLPFSDQDLAYDKQA
HHHHCCEEECCCCEEECCCCCCCCCCEEEEECCCHHHHHHCCCEEECCCCCCCCHHHHHH
VQRVYGLGIVKGNENNEFMPKGSATRGESAAFINRMLEVIESN
HHHHHHCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MICVLSSMLKKGFYNVLAASIVFSMVTIPNYSYANELDKTVTVSPDEQALKSIENHMKDE
CEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCHHHHHHHHHHHHCCC
DGRGEDKGVRNEVQGEFLVHIVKEVPLYDSSNFQKETGVRISNQVVKAEKRKGNAYYVQT
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCEEEEEE
SSGTGWIQNSDGNVEVKEIHPLLSEKLIVNEETSTYSEPFASYKEENVLEPQTIQAIGQA
CCCCCEEECCCCCEEHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHCCC
GEWFQVKINNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPIEEKTTDIYGVP
CCEEEEEECCCEEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEECCC
LKEMIVPKGNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQATGTEDRSASWH
HHHHCCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCEEEEEECCCCCCCCCCEEE
FTVDDKEIYQHLPLNENAWHAGDGAEGTGNRESIAIEIAVNEDGDYNKAVENARKLAAYL
EEECHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHH
MGELNIPLENVKKHQFWSGKICPAIMIKNNGWEPFLQGTKMYYDANHKDDITGGWYEAAI
HHCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCHHHCCCEEEECCCCCCCCCCHHHHHHH
RELDKRGIMVGDGKGSYWPERLVTRGEFANFISRSLQLPEGSSNFSDLNAAHPSLIDGIN
HHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
RAASAGIISGRGNGIFAPNDTIKRDEVVIMIDRALQYKKIKGNLVPLPFSDQDLAYDKQA
HHHHCCEEECCCCEEECCCCCCCCCCEEEEECCCHHHHHHCCCEEECCCCCCCCHHHHHH
VQRVYGLGIVKGNENNEFMPKGSATRGESAAFINRMLEVIESN
HHHHHHCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]