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The map label for this gene is lexA [H]

Identifier: 227813477

GI number: 227813477

Start: 784184

End: 784855

Strand: Reverse

Name: lexA [H]

Synonym: BAMEG_0881

Alternate gene names: 227813477

Gene position: 784855-784184 (Counterclockwise)

Preceding gene: 227813480

Following gene: 227813468

Centisome position: 15.01

GC content: 38.54

Gene sequence:

>672_bases
ATGTTATACTTATTAATAAGAAAAATGAAAGAAAACGAGGTGTTAGAAAACATGGAAAAGTTAACGAAACGCCAGCAAGA
CATTCTCGACTTTATTAAGCTAAAAGTACAAGAAAAAGGATATCCACCCTCCGTACGTGAAATCGGTCAAGCAGTCGGCC
TCGCTTCTAGTTCTACAGTGCACGGACATTTATCAAGATTAGAAGAAAAAGGATACATTCGACGCGATCCAACAAAACCA
CGTGCAATTGAAATTTTAGGTGAAGACCGAATGGATACAGAAACACAATCTGTTATTCAAGTTCCAATTGTCGGAAAAGT
TACTGCCGGTTTACCAATTACAGCGGTCGAAAGCGTAGAGGAGCACTTCCCTCTTCCAGCTAGCATTGTCGCAGGAGCAG
ATCAAGTGTTTATGTTACGTATTTCCGGGGATAGTATGATTGAGGCTGGCATTTTCGATGGAGATTTAGTTGTTGTTCGC
CAACAACAGTCTGCATATAATGGTGAAATTGTAGTCGCTTTAACAGAAGATAATGAAGCAACTGTTAAACGTTTCTATAA
AGAAAAAGACCATTTCCGTCTACAACCGGAAAACTCTTCATTAGAACCTATCATTTTAAAGCAAGTGTCAGTTATCGGTA
AAGTAATTGGCGTATATCGTGATTTACATTAA

Upstream 100 bases:

>100_bases
TTGCATTCATTATGAACCCCTCTTTCTTACTATTATTTCCCTTCAGTGTAACATAAATTCGCCTTTTGTTCGAACTTATG
TTTGTAGAATGTTTTTTCAC

Downstream 100 bases:

>100_bases
GAATATAAAAGAAGATAATTACCTTATTTATATACGGTAATTATCTTCTTTTTTTGTATTTTGATAGATTTTCACACATT
CTATTTCTTATGAACGCGTT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MLYLLIRKMKENEVLENMEKLTKRQQDILDFIKLKVQEKGYPPSVREIGQAVGLASSSTVHGHLSRLEEKGYIRRDPTKP
RAIEILGEDRMDTETQSVIQVPIVGKVTAGLPITAVESVEEHFPLPASIVAGADQVFMLRISGDSMIEAGIFDGDLVVVR
QQQSAYNGEIVVALTEDNEATVKRFYKEKDHFRLQPENSSLEPIILKQVSVIGKVIGVYRDLH

Sequences:

>Translated_223_residues
MLYLLIRKMKENEVLENMEKLTKRQQDILDFIKLKVQEKGYPPSVREIGQAVGLASSSTVHGHLSRLEEKGYIRRDPTKP
RAIEILGEDRMDTETQSVIQVPIVGKVTAGLPITAVESVEEHFPLPASIVAGADQVFMLRISGDSMIEAGIFDGDLVVVR
QQQSAYNGEIVVALTEDNEATVKRFYKEKDHFRLQPENSSLEPIILKQVSVIGKVIGVYRDLH
>Mature_223_residues
MLYLLIRKMKENEVLENMEKLTKRQQDILDFIKLKVQEKGYPPSVREIGQAVGLASSSTVHGHLSRLEEKGYIRRDPTKP
RAIEILGEDRMDTETQSVIQVPIVGKVTAGLPITAVESVEEHFPLPASIVAGADQVFMLRISGDSMIEAGIFDGDLVVVR
QQQSAYNGEIVVALTEDNEATVKRFYKEKDHFRLQPENSSLEPIILKQVSVIGKVIGVYRDLH

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family [H]

Homologues:

Organism=Escherichia coli, GI1790476, Length=209, Percent_Identity=38.2775119617225, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787431, Length=117, Percent_Identity=35.042735042735, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24 [H]

EC number: =3.4.21.88 [H]

Molecular weight: Translated: 25009; Mature: 25009

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYLLIRKMKENEVLENMEKLTKRQQDILDFIKLKVQEKGYPPSVREIGQAVGLASSSTV
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHH
HGHLSRLEEKGYIRRDPTKPRAIEILGEDRMDTETQSVIQVPIVGKVTAGLPITAVESVE
HHHHHHHHHCCCEECCCCCCCEEEEECCCCCCCCCHHEEEECCEEEECCCCCHHHHHHHH
EHFPLPASIVAGADQVFMLRISGDSMIEAGIFDGDLVVVRQQQSAYNGEIVVALTEDNEA
HHCCCCHHHHCCCCEEEEEEECCCCEEECCCCCCCEEEEEECCCCCCCEEEEEEECCCHH
TVKRFYKEKDHFRLQPENSSLEPIILKQVSVIGKVIGVYRDLH
HHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLYLLIRKMKENEVLENMEKLTKRQQDILDFIKLKVQEKGYPPSVREIGQAVGLASSSTV
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHH
HGHLSRLEEKGYIRRDPTKPRAIEILGEDRMDTETQSVIQVPIVGKVTAGLPITAVESVE
HHHHHHHHHCCCEECCCCCCCEEEEECCCCCCCCCHHEEEECCEEEECCCCCHHHHHHHH
EHFPLPASIVAGADQVFMLRISGDSMIEAGIFDGDLVVVRQQQSAYNGEIVVALTEDNEA
HHCCCCHHHHCCCCEEEEEEECCCCEEECCCCCCCEEEEEECCCCCCCEEEEEEECCCHH
TVKRFYKEKDHFRLQPENSSLEPIILKQVSVIGKVIGVYRDLH
HHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA