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The map label for this gene is rasP [H]

Identifier: 227813272

GI number: 227813272

Start: 601678

End: 602934

Strand: Direct

Name: rasP [H]

Synonym: BAMEG_0674

Alternate gene names: 227813272

Gene position: 601678-602934 (Clockwise)

Preceding gene: 227813271

Following gene: 227813273

Centisome position: 11.5

GC content: 36.75

Gene sequence:

>1257_bases
TTGAATACAGCGATTGCCTTTATATTAATTTTCGGTGCACTCGTATTTTTCCATGAGCTAGGGCATCTATATTTCGCAAA
AAGAGCAGGTATTTTATGCCGCGAGTTTGCGATTGGTTTTGGTCCGAAAATATTCTCATTTGAAAAGAATGAAACGGTGT
ATACGATTCGATTACTGCCCCTTGGTGGCTATGTAAGAATGGCTGGCGAGGATGCAGACACAGTTGAGTTAAAGCCTGGG
AAAAAGGTTGGCCTTGTATTAAATGCAAAAGACGAAGTTGCAAAATTAGTTTTTGATGGATATGAAAAGTATCCCAATGT
TCGCGTTATCGAAGTCGAACAAGCTGATTTAGAGCATAATTTAACAATTTCGGGTTATGAAGAGTACGAGGAAGAGCTTC
AAACATTCCGAGTGAACGAGAAGGCTCGTATTATTACTGCCGGTGAAGAAATTCAAATTGCTCCGTATAATAGGCAGTTT
GGCTCTAAGAAATTAGGACAACGTGCGTTAACAATCTTTGCGGGCCCTGCAATGAACTTCATTTTAGCATTTGTTATTTT
TGTGATTCTTGGATTTGTACAAGGTGTTCCTGTTGACAAACCAATGGTCGGAAAAGTGATGGAGAATAGTGCAGCAGAGC
AAGCTGGATTAAAAGAAAATGATACAATTCAAGCTATTGATGGGAAAAACACAAGTACATGGAAAGATGTTGTTACCATT
GTACGTGAAAATCCAAATAAAGAAATTACGTTACAAGTAAAGCGTGATAGTGAACAGTTTAATGTGAAAGTAACGCCAAC
GCTTGATAAAGAAGGTAAAGAAGAAGTTGGTAGAATTGGTGTTTACTCTCCTGTAGAGAAAACAGTGATGGGTTCTATTA
AATCAGGTTTTGAACAAACATACCAATGGACGAAACTAATTTTTGAGTCTTTTGTGAAATTAGTAACTGGTCAATTCTCT
ATTAATGAGTTGTCAGGTCCAGTAGGAATTTATAATCTAACAGATCAAGTTGTAGATTATGGATTTACGCGTGTTTTAAG
TTTAGCAGCCGTTTTAAGTATTAACCTTGGTTTATTTAATTTACTACCAGTTCCTGCTTTAGACGGTGGACGTTTGTTCT
TCTTCTTAATTGAGGCATTGCGTGGGAAACCAATTGATCGCCAGAAAGAAGGAATGGTTCACTTTATTGGTTTTGCATTA
TTAATGTTACTTATGTTAGTTGTAACTTGGAATGACATTCGTAAGTTTTTCTTGTAA

Upstream 100 bases:

>100_bases
CACAATGTCATTGCACGTCCGAGCTTAGAGGAAATTCTGGAAATTGATGCAGCCACAAGACGGTTTGTGATGGAACAAAT
TTAGCAAAGGTGGTATGGAA

Downstream 100 bases:

>100_bases
AATAAAGTGAAACTTATAATGAAATAGAGCTCGTAGAATAAAATCCCTCACCTAACTCGAGGTGAGGGGTTTTCCTGCGT
GTAAGAGTAAAATGAAGAGG

Product: RIP metalloprotease RasP

Products: NA

Alternate protein names: Regulating alternative sigma factor protease; Regulating anti-sigma-W factor activity protease [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MNTAIAFILIFGALVFFHELGHLYFAKRAGILCREFAIGFGPKIFSFEKNETVYTIRLLPLGGYVRMAGEDADTVELKPG
KKVGLVLNAKDEVAKLVFDGYEKYPNVRVIEVEQADLEHNLTISGYEEYEEELQTFRVNEKARIITAGEEIQIAPYNRQF
GSKKLGQRALTIFAGPAMNFILAFVIFVILGFVQGVPVDKPMVGKVMENSAAEQAGLKENDTIQAIDGKNTSTWKDVVTI
VRENPNKEITLQVKRDSEQFNVKVTPTLDKEGKEEVGRIGVYSPVEKTVMGSIKSGFEQTYQWTKLIFESFVKLVTGQFS
INELSGPVGIYNLTDQVVDYGFTRVLSLAAVLSINLGLFNLLPVPALDGGRLFFFLIEALRGKPIDRQKEGMVHFIGFAL
LMLLMLVVTWNDIRKFFL

Sequences:

>Translated_418_residues
MNTAIAFILIFGALVFFHELGHLYFAKRAGILCREFAIGFGPKIFSFEKNETVYTIRLLPLGGYVRMAGEDADTVELKPG
KKVGLVLNAKDEVAKLVFDGYEKYPNVRVIEVEQADLEHNLTISGYEEYEEELQTFRVNEKARIITAGEEIQIAPYNRQF
GSKKLGQRALTIFAGPAMNFILAFVIFVILGFVQGVPVDKPMVGKVMENSAAEQAGLKENDTIQAIDGKNTSTWKDVVTI
VRENPNKEITLQVKRDSEQFNVKVTPTLDKEGKEEVGRIGVYSPVEKTVMGSIKSGFEQTYQWTKLIFESFVKLVTGQFS
INELSGPVGIYNLTDQVVDYGFTRVLSLAAVLSINLGLFNLLPVPALDGGRLFFFLIEALRGKPIDRQKEGMVHFIGFAL
LMLLMLVVTWNDIRKFFL
>Mature_418_residues
MNTAIAFILIFGALVFFHELGHLYFAKRAGILCREFAIGFGPKIFSFEKNETVYTIRLLPLGGYVRMAGEDADTVELKPG
KKVGLVLNAKDEVAKLVFDGYEKYPNVRVIEVEQADLEHNLTISGYEEYEEELQTFRVNEKARIITAGEEIQIAPYNRQF
GSKKLGQRALTIFAGPAMNFILAFVIFVILGFVQGVPVDKPMVGKVMENSAAEQAGLKENDTIQAIDGKNTSTWKDVVTI
VRENPNKEITLQVKRDSEQFNVKVTPTLDKEGKEEVGRIGVYSPVEKTVMGSIKSGFEQTYQWTKLIFESFVKLVTGQFS
INELSGPVGIYNLTDQVVDYGFTRVLSLAAVLSINLGLFNLLPVPALDGGRLFFFLIEALRGKPIDRQKEGMVHFIGFAL
LMLLMLVVTWNDIRKFFL

Specific function: Is responsible for Site-2 cleavage of the RsiW anti- sigma factor. This results, after a third proteolytic step catalyzed by the ClpXP protease, in the release of SigW and the transcription activation of the genes under the control of the sigma-W factor [

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1786373, Length=448, Percent_Identity=29.4642857142857, Blast_Score=164, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915 [H]

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 46836; Mature: 46836

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTAIAFILIFGALVFFHELGHLYFAKRAGILCREFAIGFGPKIFSFEKNETVYTIRLLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCEEEEEEEEE
LGGYVRMAGEDADTVELKPGKKVGLVLNAKDEVAKLVFDGYEKYPNVRVIEVEQADLEHN
CCCEEEECCCCCCEEEECCCCEEEEEEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
LTISGYEEYEEELQTFRVNEKARIITAGEEIQIAPYNRQFGSKKLGQRALTIFAGPAMNF
CEECCHHHHHHHHHHEECCCCEEEEECCCEEEEECCCCCCCHHHHHHHEEEEEECHHHHH
ILAFVIFVILGFVQGVPVDKPMVGKVMENSAAEQAGLKENDTIQAIDGKNTSTWKDVVTI
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHH
VRENPNKEITLQVKRDSEQFNVKVTPTLDKEGKEEVGRIGVYSPVEKTVMGSIKSGFEQT
HHCCCCCEEEEEEEECCCEEEEEEECCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHH
YQWTKLIFESFVKLVTGQFSINELSGPVGIYNLTDQVVDYGFTRVLSLAAVLSINLGLFN
HHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LLPVPALDGGRLFFFLIEALRGKPIDRQKEGMVHFIGFALLMLLMLVVTWNDIRKFFL
HCCCCCCCCCHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTAIAFILIFGALVFFHELGHLYFAKRAGILCREFAIGFGPKIFSFEKNETVYTIRLLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCEEEEEEEEE
LGGYVRMAGEDADTVELKPGKKVGLVLNAKDEVAKLVFDGYEKYPNVRVIEVEQADLEHN
CCCEEEECCCCCCEEEECCCCEEEEEEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
LTISGYEEYEEELQTFRVNEKARIITAGEEIQIAPYNRQFGSKKLGQRALTIFAGPAMNF
CEECCHHHHHHHHHHEECCCCEEEEECCCEEEEECCCCCCCHHHHHHHEEEEEECHHHHH
ILAFVIFVILGFVQGVPVDKPMVGKVMENSAAEQAGLKENDTIQAIDGKNTSTWKDVVTI
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHH
VRENPNKEITLQVKRDSEQFNVKVTPTLDKEGKEEVGRIGVYSPVEKTVMGSIKSGFEQT
HHCCCCCEEEEEEEECCCEEEEEEECCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHH
YQWTKLIFESFVKLVTGQFSINELSGPVGIYNLTDQVVDYGFTRVLSLAAVLSINLGLFN
HHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LLPVPALDGGRLFFFLIEALRGKPIDRQKEGMVHFIGFALLMLLMLVVTWNDIRKFFL
HCCCCCCCCCHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]