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The map label for this gene is yaaJ [H]

Identifier: 227082480

GI number: 227082480

Start: 2448814

End: 2450244

Strand: Reverse

Name: yaaJ [H]

Synonym: VCM66_2279

Alternate gene names: 227082480

Gene position: 2450244-2448814 (Counterclockwise)

Preceding gene: 227082482

Following gene: 227082479

Centisome position: 84.71

GC content: 48.71

Gene sequence:

>1431_bases
GTGACAGACTTAATTAATTTAATGAATGACCTACTATGGGGTTCCATCTTGGTATACCTATTGGTCGGGGTCGGTATCTA
CTTCACTTTGCGATTGGGTTTTATCCAATTTCGTCATTTCACCCACACTTTTACCGTCCTCAAAAATAGCCGTAAAGCGG
ATAACGCTGGCATCTCCTCTTTCCAAGCGTTATGTACCAGCCTTGCCGCACGTGTCGGTACGGGGAACATGGCCGGGGTG
GCGGTTGCTTTAACCGCTGGTGGTCCTGGCGCCATCTTCTGGATGTGGTTGATTGCCATGTTAGGCATGGCGACTTCTTT
CGCTGAAAGTACCCTAGCTCAGTTGTATAAAACGCGTGACAAAGATGGCAATTACCGCGGCGGTCCAGCGTATTACATGG
AAAAAGGCCTTGGTATGCGTTGGATGGGGGTGCTGTTCTCCATCTTCCTGATCATTGCGTTTGGTTTGGTGTTTAACGCG
GTGCAAGCCAACTCAATCACCAATGCGATGAAAACGGCTTTCGGTTTTGAACCCATGCTGATCGGTATTGGTATCGTGTT
GCTGACCGCTTTCGTGATTTTTGGTGGCATCCGTAAGATTGCACGGACGGCAGAGCTGATTGTGCCTTTCATGGCGTTTG
CTTATCTGGCGATTGCTCTGTTTGTCGTGGTCATGAATTATGAGAAAGTGCCTGATGCGATTAGCCTGATTTTCAAAAGT
GCCTTCGGTTTACAAGAAGCGGCTGCAGGTGGCCTAGGCTATGCGATTGCACAAGCTATGATCAACGGTGTGAAACGTGG
CTTGTTCTCAAACGAAGCGGGTATGGGTTCTGCACCTAACGCCGCGGCTTCTGCAACGCCATATCCACCACACCCAGCTT
CACAAGGTTACGTGCAAATGCTTGGTGTGTTCATCGATACGATTGTGATCTGTACCGCGACAGTGGCGATTATCCTTATG
TCTGGTGAGTATGTACCGCACGGCGAGTTAACGGGTATTGAGCTGACTCAGCGTGCACTCAGTTCACAAGTAGGGGATTG
GGGAGGTATCTTTATCGCATTTGCGATCTTCTTCTTCGCTTTTACTTCGATCATCGCCAACTACTCGTATGCGGAAACCA
ACCTGATTTTCCTTGAGCACAACAATAAGAAAGGCTTAGTGCTGTTTCGTCTCGTGTTCTTAGGTATGGTGATGTTTGGC
TCGTTGGCAACATTGCCAACGGTGTGGGCGATGGCGGATGTCTCGATGGGCTTGATGGCGATTGTGAACTTGGTCGCTAT
CCTCCTGCTCTCAGGTATCGTGATTAAACTGGCGAAAGATTACAACCGTCAGCTGGATGCTGGCAAAGTACCGACCTTTG
ATGCCAATGATTACCCAGAGTTGAAATCTCAGCTTGAAGATGGCATTTGGGATAACAACAAAGCGTCATAG

Upstream 100 bases:

>100_bases
TGACATACACGGCAAAAAGATAATCTCAATACCGTATCCGTGTAACAGAAACTCTTACTCATCGGAGTCAGAGCAGCTCA
TCACTCTATGCGAGGTTCCT

Downstream 100 bases:

>100_bases
ACAGCGCCGATGTCAGAAATTGAAAAAGCCATGCAGACACCGCATGGCTTTTTTTGTACCCTAGCTTGCGTCATCTTATA
AAAAATTTAATCACTTGGAA

Product: sodium/alanine symporter

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTDLINLMNDLLWGSILVYLLVGVGIYFTLRLGFIQFRHFTHTFTVLKNSRKADNAGISSFQALCTSLAARVGTGNMAGV
AVALTAGGPGAIFWMWLIAMLGMATSFAESTLAQLYKTRDKDGNYRGGPAYYMEKGLGMRWMGVLFSIFLIIAFGLVFNA
VQANSITNAMKTAFGFEPMLIGIGIVLLTAFVIFGGIRKIARTAELIVPFMAFAYLAIALFVVVMNYEKVPDAISLIFKS
AFGLQEAAAGGLGYAIAQAMINGVKRGLFSNEAGMGSAPNAAASATPYPPHPASQGYVQMLGVFIDTIVICTATVAIILM
SGEYVPHGELTGIELTQRALSSQVGDWGGIFIAFAIFFFAFTSIIANYSYAETNLIFLEHNNKKGLVLFRLVFLGMVMFG
SLATLPTVWAMADVSMGLMAIVNLVAILLLSGIVIKLAKDYNRQLDAGKVPTFDANDYPELKSQLEDGIWDNNKAS

Sequences:

>Translated_476_residues
MTDLINLMNDLLWGSILVYLLVGVGIYFTLRLGFIQFRHFTHTFTVLKNSRKADNAGISSFQALCTSLAARVGTGNMAGV
AVALTAGGPGAIFWMWLIAMLGMATSFAESTLAQLYKTRDKDGNYRGGPAYYMEKGLGMRWMGVLFSIFLIIAFGLVFNA
VQANSITNAMKTAFGFEPMLIGIGIVLLTAFVIFGGIRKIARTAELIVPFMAFAYLAIALFVVVMNYEKVPDAISLIFKS
AFGLQEAAAGGLGYAIAQAMINGVKRGLFSNEAGMGSAPNAAASATPYPPHPASQGYVQMLGVFIDTIVICTATVAIILM
SGEYVPHGELTGIELTQRALSSQVGDWGGIFIAFAIFFFAFTSIIANYSYAETNLIFLEHNNKKGLVLFRLVFLGMVMFG
SLATLPTVWAMADVSMGLMAIVNLVAILLLSGIVIKLAKDYNRQLDAGKVPTFDANDYPELKSQLEDGIWDNNKAS
>Mature_475_residues
TDLINLMNDLLWGSILVYLLVGVGIYFTLRLGFIQFRHFTHTFTVLKNSRKADNAGISSFQALCTSLAARVGTGNMAGVA
VALTAGGPGAIFWMWLIAMLGMATSFAESTLAQLYKTRDKDGNYRGGPAYYMEKGLGMRWMGVLFSIFLIIAFGLVFNAV
QANSITNAMKTAFGFEPMLIGIGIVLLTAFVIFGGIRKIARTAELIVPFMAFAYLAIALFVVVMNYEKVPDAISLIFKSA
FGLQEAAAGGLGYAIAQAMINGVKRGLFSNEAGMGSAPNAAASATPYPPHPASQGYVQMLGVFIDTIVICTATVAIILMS
GEYVPHGELTGIELTQRALSSQVGDWGGIFIAFAIFFFAFTSIIANYSYAETNLIFLEHNNKKGLVLFRLVFLGMVMFGS
LATLPTVWAMADVSMGLMAIVNLVAILLLSGIVIKLAKDYNRQLDAGKVPTFDANDYPELKSQLEDGIWDNNKAS

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=472, Percent_Identity=51.6949152542373, Blast_Score=481, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 51342; Mature: 51211

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TDLINLMNDLLWGSILVYLLVGVGIYFTLRLGFIQFRHFTHTFTVLKNSRKADNAGISS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
FQALCTSLAARVGTGNMAGVAVALTAGGPGAIFWMWLIAMLGMATSFAESTLAQLYKTRD
HHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KDGNYRGGPAYYMEKGLGMRWMGVLFSIFLIIAFGLVFNAVQANSITNAMKTAFGFEPML
CCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IGIGIVLLTAFVIFGGIRKIARTAELIVPFMAFAYLAIALFVVVMNYEKVPDAISLIFKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
AFGLQEAAAGGLGYAIAQAMINGVKRGLFSNEAGMGSAPNAAASATPYPPHPASQGYVQM
HHCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
LGVFIDTIVICTATVAIILMSGEYVPHGELTGIELTQRALSSQVGDWGGIFIAFAIFFFA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FTSIIANYSYAETNLIFLEHNNKKGLVLFRLVFLGMVMFGSLATLPTVWAMADVSMGLMA
HHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVNLVAILLLSGIVIKLAKDYNRQLDAGKVPTFDANDYPELKSQLEDGIWDNNKAS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503 [H]