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The map label for this gene is dctD-1 [H]

Identifier: 227082056

GI number: 227082056

Start: 2014555

End: 2015895

Strand: Direct

Name: dctD-1 [H]

Synonym: VCM66_1850

Alternate gene names: 227082056

Gene position: 2014555-2015895 (Clockwise)

Preceding gene: 227082055

Following gene: 227082063

Centisome position: 69.65

GC content: 49.52

Gene sequence:

>1341_bases
ATGTGTGATGTATTTTTGATTGATGATGAGAGCGATATCCGCATTGCGCTCGCTCAAAGTTTCGAATTGGCAGACCTCAA
CGCGCAGTTTTTTGCCAGTGTCGAAGAGGCACTGCTGGCAATCAAGGCGCAAGGACTCCCATTGGTGATCGTCAGTGATA
TCTGCTTACCCGGTTTAAGTGGACAGAATTTACTGAGCTCCGTATTACATCAAGACAGTGAACTGCCTGTCATTCTTATC
ACTGGACATGGCGATATTTCGATGGCGGTCAAAGCCATGCATGATGGTGCTTACGATTTTATTGAAAAGCCCTTCGCGCC
TGAGAGGTTGATTGACACGGTACACCGTGCCATCGAAAAACGTCGCCTTACTTTGGAGAACCGTCAGCTAAAACGCAGTT
TAAAAGTCAGCCAAACCTTAGGCCCGCGAATTATTGGTGAAACCGCCGCCATTCAAACTCTGCGCGATACCATTGCCCAA
GTCGCGGATACCCAAGCGGATATTTTATTGTTTGGTGAAACAGGAACCGGCAAGGAGTTGGTTGCCCGTTCACTGCATGA
ACAGAGCTCGCGCCGTGAACAGAATTTTGTTGCGATTAACTGTGGTGCGGTGCCGGAGAACTTGATTGAGAGCGAGCTCT
ACGGCCATGAAAAAGGCGCGTTTACGGGGGCAGACAGCCGTCGAGTGGGTAAGTTTGAGCATGCTCAAGGTGGCACTCTG
TTTTTAGATGAGATTGAATCGATGCCGATGCAAGCGCAGATCCGGCTGCTGCGTGTGCTGCAAGAGCGAGTGATCGAACG
GATTGGCTCGAATGAACTCATTCCCCTTGATATTCGGGTGATTGCCGCCACTAAAATCGATTTAAAACAAGCCGCTGCCG
AAGGGAAATTTCGGCAAGATCTCTACTATCGGCTCAACATTGTCACCTTAACGATCCCACCGCTGCGTGAGCGCAGAGAG
GACATTCCTGCCCTTTTCCACCATTTCTTACTGGTCGCTGCCGCACGTTATGGTAAAGCGGCTACAGCACTGACGGCCTC
CGATGTGCAAAGCTTGCTTAGTCACGATTGGCCGGGCAACGTGCGGGAATTGCGTAATGCCGCAGAACGCTATGTGTTAC
TCGGTAAGTTGGCGCAATTTGCTGAAAGCCATGAGCCAAAATCCAAATTACTCGGACTGAATGAACAAGTCGCGGAGTTT
GAGAAATCACTTTTGGAGCAGACGCTGATTGAGTGTGGTGGCAGCATTAAAGCCACCATGGAGCGTCTCCACCTTCCACG
TAAAACCTTGTATGACAAAATGCAGAAATATCAGCTCGATAAAGAGAGTTACAAACCCTGA

Upstream 100 bases:

>100_bases
ACAGCATGCAGGGAACATTGAGCGTGGCGACTTCACCACTCGGTGGAGCGCGCTTTACCATCACCCTGCCTCGTTTCACA
CCTCTCAATATGGACTAACC

Downstream 100 bases:

>100_bases
AACCATCCCGCGTTCTGGTTAAAAGTCTAGGTCTCAGATTAAAGGTCGGCATAAAAAAACCAAGCCCTTCGCATGCGAAG
GGCTTGTCGTTATTTGCAAA

Product: putative C4-dicarboxylate transport transcriptional regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MCDVFLIDDESDIRIALAQSFELADLNAQFFASVEEALLAIKAQGLPLVIVSDICLPGLSGQNLLSSVLHQDSELPVILI
TGHGDISMAVKAMHDGAYDFIEKPFAPERLIDTVHRAIEKRRLTLENRQLKRSLKVSQTLGPRIIGETAAIQTLRDTIAQ
VADTQADILLFGETGTGKELVARSLHEQSSRREQNFVAINCGAVPENLIESELYGHEKGAFTGADSRRVGKFEHAQGGTL
FLDEIESMPMQAQIRLLRVLQERVIERIGSNELIPLDIRVIAATKIDLKQAAAEGKFRQDLYYRLNIVTLTIPPLRERRE
DIPALFHHFLLVAAARYGKAATALTASDVQSLLSHDWPGNVRELRNAAERYVLLGKLAQFAESHEPKSKLLGLNEQVAEF
EKSLLEQTLIECGGSIKATMERLHLPRKTLYDKMQKYQLDKESYKP

Sequences:

>Translated_446_residues
MCDVFLIDDESDIRIALAQSFELADLNAQFFASVEEALLAIKAQGLPLVIVSDICLPGLSGQNLLSSVLHQDSELPVILI
TGHGDISMAVKAMHDGAYDFIEKPFAPERLIDTVHRAIEKRRLTLENRQLKRSLKVSQTLGPRIIGETAAIQTLRDTIAQ
VADTQADILLFGETGTGKELVARSLHEQSSRREQNFVAINCGAVPENLIESELYGHEKGAFTGADSRRVGKFEHAQGGTL
FLDEIESMPMQAQIRLLRVLQERVIERIGSNELIPLDIRVIAATKIDLKQAAAEGKFRQDLYYRLNIVTLTIPPLRERRE
DIPALFHHFLLVAAARYGKAATALTASDVQSLLSHDWPGNVRELRNAAERYVLLGKLAQFAESHEPKSKLLGLNEQVAEF
EKSLLEQTLIECGGSIKATMERLHLPRKTLYDKMQKYQLDKESYKP
>Mature_446_residues
MCDVFLIDDESDIRIALAQSFELADLNAQFFASVEEALLAIKAQGLPLVIVSDICLPGLSGQNLLSSVLHQDSELPVILI
TGHGDISMAVKAMHDGAYDFIEKPFAPERLIDTVHRAIEKRRLTLENRQLKRSLKVSQTLGPRIIGETAAIQTLRDTIAQ
VADTQADILLFGETGTGKELVARSLHEQSSRREQNFVAINCGAVPENLIESELYGHEKGAFTGADSRRVGKFEHAQGGTL
FLDEIESMPMQAQIRLLRVLQERVIERIGSNELIPLDIRVIAATKIDLKQAAAEGKFRQDLYYRLNIVTLTIPPLRERRE
DIPALFHHFLLVAAARYGKAATALTASDVQSLLSHDWPGNVRELRNAAERYVLLGKLAQFAESHEPKSKLLGLNEQVAEF
EKSLLEQTLIECGGSIKATMERLHLPRKTLYDKMQKYQLDKESYKP

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=454, Percent_Identity=38.5462555066079, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI1790299, Length=373, Percent_Identity=43.9678284182306, Blast_Score=304, Evalue=8e-84,
Organism=Escherichia coli, GI1790437, Length=442, Percent_Identity=38.4615384615385, Blast_Score=292, Evalue=2e-80,
Organism=Escherichia coli, GI1788905, Length=403, Percent_Identity=36.7245657568238, Blast_Score=267, Evalue=9e-73,
Organism=Escherichia coli, GI1789087, Length=343, Percent_Identity=41.9825072886297, Blast_Score=260, Evalue=2e-70,
Organism=Escherichia coli, GI87082117, Length=333, Percent_Identity=43.8438438438438, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1789233, Length=319, Percent_Identity=41.0658307210031, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1786524, Length=276, Percent_Identity=43.1159420289855, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI87082152, Length=241, Percent_Identity=45.643153526971, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI87081872, Length=231, Percent_Identity=42.4242424242424, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1787583, Length=333, Percent_Identity=33.033033033033, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87081858, Length=300, Percent_Identity=32.3333333333333, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI1789828, Length=233, Percent_Identity=39.4849785407725, Blast_Score=138, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49779; Mature: 49779

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCDVFLIDDESDIRIALAQSFELADLNAQFFASVEEALLAIKAQGLPLVIVSDICLPGLS
CCEEEEEECCCCCEEEEECCCCEECCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCC
GQNLLSSVLHQDSELPVILITGHGDISMAVKAMHDGAYDFIEKPFAPERLIDTVHRAIEK
CHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHH
RRLTLENRQLKRSLKVSQTLGPRIIGETAAIQTLRDTIAQVADTQADILLFGETGTGKEL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHH
VARSLHEQSSRREQNFVAINCGAVPENLIESELYGHEKGAFTGADSRRVGKFEHAQGGTL
HHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEE
FLDEIESMPMQAQIRLLRVLQERVIERIGSNELIPLDIRVIAATKIDLKQAAAEGKFRQD
EHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEHHHHHHHHHCCCHHHH
LYYRLNIVTLTIPPLRERREDIPALFHHFLLVAAARYGKAATALTASDVQSLLSHDWPGN
HEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
VRELRNAAERYVLLGKLAQFAESHEPKSKLLGLNEQVAEFEKSLLEQTLIECGGSIKATM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
ERLHLPRKTLYDKMQKYQLDKESYKP
HHHCCCHHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure
MCDVFLIDDESDIRIALAQSFELADLNAQFFASVEEALLAIKAQGLPLVIVSDICLPGLS
CCEEEEEECCCCCEEEEECCCCEECCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCC
GQNLLSSVLHQDSELPVILITGHGDISMAVKAMHDGAYDFIEKPFAPERLIDTVHRAIEK
CHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHH
RRLTLENRQLKRSLKVSQTLGPRIIGETAAIQTLRDTIAQVADTQADILLFGETGTGKEL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHH
VARSLHEQSSRREQNFVAINCGAVPENLIESELYGHEKGAFTGADSRRVGKFEHAQGGTL
HHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEE
FLDEIESMPMQAQIRLLRVLQERVIERIGSNELIPLDIRVIAATKIDLKQAAAEGKFRQD
EHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEHHHHHHHHHCCCHHHH
LYYRLNIVTLTIPPLRERREDIPALFHHFLLVAAARYGKAATALTASDVQSLLSHDWPGN
HEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
VRELRNAAERYVLLGKLAQFAESHEPKSKLLGLNEQVAEFEKSLLEQTLIECGGSIKATM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
ERLHLPRKTLYDKMQKYQLDKESYKP
HHHCCCHHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]