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The map label for this gene is dctB [H]

Identifier: 227082055

GI number: 227082055

Start: 2012759

End: 2014552

Strand: Direct

Name: dctB [H]

Synonym: VCM66_1849

Alternate gene names: 227082055

Gene position: 2012759-2014552 (Clockwise)

Preceding gene: 227082054

Following gene: 227082056

Centisome position: 69.58

GC content: 49.5

Gene sequence:

>1794_bases
ATGCCATCCATTCGTCGTCTCAGCTTGATGTTGTGGGTCATGTTTATTGTGCTGGTGAGCATGAGCACGCACTTATACTG
GCGTTTCCAGTATCAAGCGCTGCTCAATGAGCATCAGTCGCAGCTTGATCGTTTTTCAAGCCATATCGTTGCGACTTTAG
ATAAATACGCCCACATTCCCCATCTGATTTCTAAAGATAAAGAGTTGGTGGATGCACTGCTCTCGGCACAAAATTCGGCG
CAAATCGACATCACCAATCGCTATTTAGAGCAAGTCAATGAAGTCATCCAAGCCGCAGATACCTATTTGATCGACCGCTT
TGGCAACACGATCGCATCCAGTAACTGGAATTTGGATCGCTCTTTTATCGGACGCAACTTTGCATGGCGACCTTATTTTT
ACCTCTCCATTGCGGGTCAAAAAAGCCAATATTTTGCGCTGGGGTCAACCTCAGGACAACGCGGCTACTATTACGCTTAT
CCAGTGATTTATGCGGCGGAAATCTTAGGGGTTATCGTGGTCAAAATGGATTTGTCGGCCATTGAGCAGGGCTGGCAAAG
CAAGAGCAGCTATTTTGTGGCGACTGATGATCATCAAGTGGTGTTTATGTCGAGCCAACCTGCTTGGCTGTTTCATAGCG
TTGCAGATTTATCTCCCGCACAGCTCAACGACATTCGCCAAAGCCAGCAATATTTAGACAGCCCTATTCCTTCCCTCGGC
TGGCAGGGCGACTTGCAAGCGGAGCAAAGCGAATGGCGCAAGCCTGAAAAACATTGGCTGCAAGATGACTACATTGTTTC
GAGTCGCCCGCTTCCTGAACTTGCCCTGACCATTCGCGTGCTTTCACCGAAAATCGAACTCTTTTGGTCGGCGTTTGGCT
TAGTGATTATTCTCTGCATGTTGTTTGCGATTGTGTATTTGGGTTTTCAACTGTGGCATCACCGGCAGCTGCGTCAGCGG
CAAATCGAGCAACTGCAGCAAGAGACCAAGCAGAAGCTTGAATTTGAGGTGATGGAGTGCACCGCCAAATTGCATGCGGA
AATTGCTGAACGGATCAAAACCGAGCACGCGCTGCGCCAAACACAAGATGAGCTGATCCAAGCGGCGAAACTGGCAGTGC
TCGGCCAAATGTCGGCCAGTATTAGCCACGAGCTGAACAATCCGCTGGCGGCGATCCGCAGTTTTGCCGATAACGGCCGC
CGTTTCCTTGCCACCAATAAGCCTGAACGCGCTGAAGAAAATCTCTCGCGTATTTCCGCACTGACGGAGCGCATGGCGAA
AATCAGTGAGCAGCTTAAATCTTTCGCCCGAAAATCCACTTCCAATGAGCAGCAAGACGTGCAGTTGTACCCGTTGATTT
TGTCGGCCAAAGAGTTGATGACTGGGCAATTTAAAGCGCAGATGACGTTGTTAGAGCTGTGCGACCTCGAAACGCCCGTT
TGGGTACGCGTCAATCCCATCCAGTTAGAACAAGTGCTGATTAATCTGCTCACCAATGCACTGCAAGCGTTAGAGGGACA
AGCGGAGCGCGTAGTTCAAATCCGTTTGCAAACCTCTGAACAGCAAATAGCGCTTTTCGTTGATGATAATGGTCCCGGTG
TCGGCATTGAAACCTTGCCCCATCTGTTTGAACCCTTCCACACCACGAAAAAGAATGGTTTAGGGCTTGGACTTTCTATT
TCACAGCAAATTTTGCACAGCATGCAGGGAACATTGAGCGTGGCGACTTCACCACTCGGTGGAGCGCGCTTTACCATCAC
CCTGCCTCGTTTCACACCTCTCAATATGGACTAA

Upstream 100 bases:

>100_bases
TTGCTCTTGTTTTGCAGACCGAGATTGCGATCTTTTTAGACGCTTCGGCTGTGCATTCTCAGTATGATGTAACGAAAGGA
TTTGTTGATAAGAGCCAAGG

Downstream 100 bases:

>100_bases
CCATGTGTGATGTATTTTTGATTGATGATGAGAGCGATATCCGCATTGCGCTCGCTCAAAGTTTCGAATTGGCAGACCTC
AACGCGCAGTTTTTTGCCAG

Product: putative C4-dicarboxylate transport sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MPSIRRLSLMLWVMFIVLVSMSTHLYWRFQYQALLNEHQSQLDRFSSHIVATLDKYAHIPHLISKDKELVDALLSAQNSA
QIDITNRYLEQVNEVIQAADTYLIDRFGNTIASSNWNLDRSFIGRNFAWRPYFYLSIAGQKSQYFALGSTSGQRGYYYAY
PVIYAAEILGVIVVKMDLSAIEQGWQSKSSYFVATDDHQVVFMSSQPAWLFHSVADLSPAQLNDIRQSQQYLDSPIPSLG
WQGDLQAEQSEWRKPEKHWLQDDYIVSSRPLPELALTIRVLSPKIELFWSAFGLVIILCMLFAIVYLGFQLWHHRQLRQR
QIEQLQQETKQKLEFEVMECTAKLHAEIAERIKTEHALRQTQDELIQAAKLAVLGQMSASISHELNNPLAAIRSFADNGR
RFLATNKPERAEENLSRISALTERMAKISEQLKSFARKSTSNEQQDVQLYPLILSAKELMTGQFKAQMTLLELCDLETPV
WVRVNPIQLEQVLINLLTNALQALEGQAERVVQIRLQTSEQQIALFVDDNGPGVGIETLPHLFEPFHTTKKNGLGLGLSI
SQQILHSMQGTLSVATSPLGGARFTITLPRFTPLNMD

Sequences:

>Translated_597_residues
MPSIRRLSLMLWVMFIVLVSMSTHLYWRFQYQALLNEHQSQLDRFSSHIVATLDKYAHIPHLISKDKELVDALLSAQNSA
QIDITNRYLEQVNEVIQAADTYLIDRFGNTIASSNWNLDRSFIGRNFAWRPYFYLSIAGQKSQYFALGSTSGQRGYYYAY
PVIYAAEILGVIVVKMDLSAIEQGWQSKSSYFVATDDHQVVFMSSQPAWLFHSVADLSPAQLNDIRQSQQYLDSPIPSLG
WQGDLQAEQSEWRKPEKHWLQDDYIVSSRPLPELALTIRVLSPKIELFWSAFGLVIILCMLFAIVYLGFQLWHHRQLRQR
QIEQLQQETKQKLEFEVMECTAKLHAEIAERIKTEHALRQTQDELIQAAKLAVLGQMSASISHELNNPLAAIRSFADNGR
RFLATNKPERAEENLSRISALTERMAKISEQLKSFARKSTSNEQQDVQLYPLILSAKELMTGQFKAQMTLLELCDLETPV
WVRVNPIQLEQVLINLLTNALQALEGQAERVVQIRLQTSEQQIALFVDDNGPGVGIETLPHLFEPFHTTKKNGLGLGLSI
SQQILHSMQGTLSVATSPLGGARFTITLPRFTPLNMD
>Mature_596_residues
PSIRRLSLMLWVMFIVLVSMSTHLYWRFQYQALLNEHQSQLDRFSSHIVATLDKYAHIPHLISKDKELVDALLSAQNSAQ
IDITNRYLEQVNEVIQAADTYLIDRFGNTIASSNWNLDRSFIGRNFAWRPYFYLSIAGQKSQYFALGSTSGQRGYYYAYP
VIYAAEILGVIVVKMDLSAIEQGWQSKSSYFVATDDHQVVFMSSQPAWLFHSVADLSPAQLNDIRQSQQYLDSPIPSLGW
QGDLQAEQSEWRKPEKHWLQDDYIVSSRPLPELALTIRVLSPKIELFWSAFGLVIILCMLFAIVYLGFQLWHHRQLRQRQ
IEQLQQETKQKLEFEVMECTAKLHAEIAERIKTEHALRQTQDELIQAAKLAVLGQMSASISHELNNPLAAIRSFADNGRR
FLATNKPERAEENLSRISALTERMAKISEQLKSFARKSTSNEQQDVQLYPLILSAKELMTGQFKAQMTLLELCDLETPVW
VRVNPIQLEQVLINLLTNALQALEGQAERVVQIRLQTSEQQIALFVDDNGPGVGIETLPHLFEPFHTTKKNGLGLGLSIS
QQILHSMQGTLSVATSPLGGARFTITLPRFTPLNMD

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=242, Percent_Identity=36.3636363636364, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1790436, Length=230, Percent_Identity=31.304347826087, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87081816, Length=269, Percent_Identity=28.6245353159851, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI145693157, Length=262, Percent_Identity=25.5725190839695, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787894, Length=246, Percent_Identity=30.4878048780488, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788393, Length=221, Percent_Identity=28.9592760180996, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790346, Length=221, Percent_Identity=28.5067873303167, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786600, Length=285, Percent_Identity=24.5614035087719, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788713, Length=255, Percent_Identity=26.2745098039216, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1786783, Length=382, Percent_Identity=24.869109947644, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790300, Length=255, Percent_Identity=23.921568627451, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1786912, Length=222, Percent_Identity=28.3783783783784, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789808, Length=223, Percent_Identity=26.457399103139, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 68024; Mature: 67893

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSIRRLSLMLWVMFIVLVSMSTHLYWRFQYQALLNEHQSQLDRFSSHIVATLDKYAHIP
CCCHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HLISKDKELVDALLSAQNSAQIDITNRYLEQVNEVIQAADTYLIDRFGNTIASSNWNLDR
HHHHCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCH
SFIGRNFAWRPYFYLSIAGQKSQYFALGSTSGQRGYYYAYPVIYAAEILGVIVVKMDLSA
HHHCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
IEQGWQSKSSYFVATDDHQVVFMSSQPAWLFHSVADLSPAQLNDIRQSQQYLDSPIPSLG
HHHHHCCCCCEEEEECCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
WQGDLQAEQSEWRKPEKHWLQDDYIVSSRPLPELALTIRVLSPKIELFWSAFGLVIILCM
CCCCCCCCHHHCCCCHHHHCCCCEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LFAIVYLGFQLWHHRQLRQRQIEQLQQETKQKLEFEVMECTAKLHAEIAERIKTEHALRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQDELIQAAKLAVLGQMSASISHELNNPLAAIRSFADNGRRFLATNKPERAEENLSRISA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHH
LTERMAKISEQLKSFARKSTSNEQQDVQLYPLILSAKELMTGQFKAQMTLLELCDLETPV
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
WVRVNPIQLEQVLINLLTNALQALEGQAERVVQIRLQTSEQQIALFVDDNGPGVGIETLP
EEEECHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCEEEEEEECCCCCCCHHHHH
HLFEPFHTTKKNGLGLGLSISQQILHSMQGTLSVATSPLGGARFTITLPRFTPLNMD
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHEECCCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
PSIRRLSLMLWVMFIVLVSMSTHLYWRFQYQALLNEHQSQLDRFSSHIVATLDKYAHIP
CCHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HLISKDKELVDALLSAQNSAQIDITNRYLEQVNEVIQAADTYLIDRFGNTIASSNWNLDR
HHHHCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCH
SFIGRNFAWRPYFYLSIAGQKSQYFALGSTSGQRGYYYAYPVIYAAEILGVIVVKMDLSA
HHHCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
IEQGWQSKSSYFVATDDHQVVFMSSQPAWLFHSVADLSPAQLNDIRQSQQYLDSPIPSLG
HHHHHCCCCCEEEEECCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
WQGDLQAEQSEWRKPEKHWLQDDYIVSSRPLPELALTIRVLSPKIELFWSAFGLVIILCM
CCCCCCCCHHHCCCCHHHHCCCCEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LFAIVYLGFQLWHHRQLRQRQIEQLQQETKQKLEFEVMECTAKLHAEIAERIKTEHALRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQDELIQAAKLAVLGQMSASISHELNNPLAAIRSFADNGRRFLATNKPERAEENLSRISA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHH
LTERMAKISEQLKSFARKSTSNEQQDVQLYPLILSAKELMTGQFKAQMTLLELCDLETPV
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
WVRVNPIQLEQVLINLLTNALQALEGQAERVVQIRLQTSEQQIALFVDDNGPGVGIETLP
EEEECHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCEEEEEEECCCCCCCHHHHH
HLFEPFHTTKKNGLGLGLSISQQILHSMQGTLSVATSPLGGARFTITLPRFTPLNMD
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHEECCCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431; 2551890; 2695394 [H]