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The map label for this gene is clpP [H]

Identifier: 227082052

GI number: 227082052

Start: 2010227

End: 2010829

Strand: Reverse

Name: clpP [H]

Synonym: VCM66_1846

Alternate gene names: 227082052

Gene position: 2010829-2010227 (Counterclockwise)

Preceding gene: 227082053

Following gene: 227082051

Centisome position: 69.52

GC content: 46.6

Gene sequence:

>603_bases
ATGTCGCCCATTTTTGACGCACTCGTACCTATGGTGGTTGAACAAACCTCTCGTGGTGAGCGTTCATACGACATCTATTC
TCGCTTACTAAAAGAACGAGTGATTTTCTTAACCGGGCAAGTAGAAGATCACATGGCAAACCTTGTCGTGGCTCAGCTGC
TTTTCTTAGAATCTGAAAACCCTGATAAAGATATTTTCCTTTACATCAACTCACCCGGTGGCAGTGTCACTGCAGGGATG
TCGATTTACGATACTATGCAGTTCATCAAGCCAAACGTGAGCACAGTGTGTATGGGACAAGCCTGTTCAATGGGCGCTTT
CTTGCTTGCTGGTGGTGCACCGGGCAAGCGTTATGTACTGCCGAATTCTCGTGTCATGATTCACCAACCACTGGGTGGAT
TCCAAGGCCAAGCCTCTGATATTCAAATTCATGCTCAAGAAATTCTGACCATTAAAAACAAACTGAACCGTTTGTTGGCA
GAGCATACAGGCCAGCCAATCGAAGTGATTGAACGCGATACTGATCGTGATAACTTCATGTCGGCAGATCAAGCAGTGGA
ATACGGTTTGGTGGATGCGGTGCTGAAACACCGTGGCGAGTAA

Upstream 100 bases:

>100_bases
AAACAAGCAATCATCTGCTAACAATGGTCCGTGTGATACCCATCATCCGGGCCATTTATTTTAGGGATATAAGACTATGA
GCTACCAAGAAAAAAATGCA

Downstream 100 bases:

>100_bases
GCTCGTAATTGTATAACGCAATTTTGATTGAATTGTTATACACTCAAACCAAAGTGAGTAAAGGCTAAGAGGTTAGCGAA
TGACAGACAAAAGCAAAGAG

Product: ATP-dependent Clp protease proteolytic subunit

Products: NA

Alternate protein names: Endopeptidase Clp [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSPIFDALVPMVVEQTSRGERSYDIYSRLLKERVIFLTGQVEDHMANLVVAQLLFLESENPDKDIFLYINSPGGSVTAGM
SIYDTMQFIKPNVSTVCMGQACSMGAFLLAGGAPGKRYVLPNSRVMIHQPLGGFQGQASDIQIHAQEILTIKNKLNRLLA
EHTGQPIEVIERDTDRDNFMSADQAVEYGLVDAVLKHRGE

Sequences:

>Translated_200_residues
MSPIFDALVPMVVEQTSRGERSYDIYSRLLKERVIFLTGQVEDHMANLVVAQLLFLESENPDKDIFLYINSPGGSVTAGM
SIYDTMQFIKPNVSTVCMGQACSMGAFLLAGGAPGKRYVLPNSRVMIHQPLGGFQGQASDIQIHAQEILTIKNKLNRLLA
EHTGQPIEVIERDTDRDNFMSADQAVEYGLVDAVLKHRGE
>Mature_199_residues
SPIFDALVPMVVEQTSRGERSYDIYSRLLKERVIFLTGQVEDHMANLVVAQLLFLESENPDKDIFLYINSPGGSVTAGMS
IYDTMQFIKPNVSTVCMGQACSMGAFLLAGGAPGKRYVLPNSRVMIHQPLGGFQGQASDIQIHAQEILTIKNKLNRLLAE
HTGQPIEVIERDTDRDNFMSADQAVEYGLVDAVLKHRGE

Specific function: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins [H]

COG id: COG0740

COG function: function code OU; Protease subunit of ATP-dependent Clp proteases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S14 family [H]

Homologues:

Organism=Homo sapiens, GI5174419, Length=190, Percent_Identity=57.8947368421053, Blast_Score=238, Evalue=2e-63,
Organism=Escherichia coli, GI1786641, Length=192, Percent_Identity=79.1666666666667, Blast_Score=332, Evalue=8e-93,
Organism=Caenorhabditis elegans, GI17538017, Length=191, Percent_Identity=53.4031413612565, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI20129427, Length=193, Percent_Identity=59.5854922279793, Blast_Score=248, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001907
- InterPro:   IPR018215 [H]

Pfam domain/function: PF00574 CLP_protease [H]

EC number: =3.4.21.92 [H]

Molecular weight: Translated: 22131; Mature: 22000

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00381 CLP_PROTEASE_SER ; PS00382 CLP_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPIFDALVPMVVEQTSRGERSYDIYSRLLKERVIFLTGQVEDHMANLVVAQLLFLESEN
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHCCCC
PDKDIFLYINSPGGSVTAGMSIYDTMQFIKPNVSTVCMGQACSMGAFLLAGGAPGKRYVL
CCCEEEEEEECCCCCEEECHHHHHHHHHHCCCCCEEECCCHHCCCEEEEECCCCCCEEEC
PNSRVMIHQPLGGFQGQASDIQIHAQEILTIKNKLNRLLAEHTGQPIEVIERDTDRDNFM
CCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEHCCCCCCHHHH
SADQAVEYGLVDAVLKHRGE
CHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SPIFDALVPMVVEQTSRGERSYDIYSRLLKERVIFLTGQVEDHMANLVVAQLLFLESEN
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHCCCC
PDKDIFLYINSPGGSVTAGMSIYDTMQFIKPNVSTVCMGQACSMGAFLLAGGAPGKRYVL
CCCEEEEEEECCCCCEEECHHHHHHHHHHCCCCCEEECCCHHCCCEEEEECCCCCCEEEC
PNSRVMIHQPLGGFQGQASDIQIHAQEILTIKNKLNRLLAEHTGQPIEVIERDTDRDNFM
CCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEHCCCCCCHHHH
SADQAVEYGLVDAVLKHRGE
CHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA