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The map label for this gene is miaE [H]

Identifier: 227082040

GI number: 227082040

Start: 1997062

End: 1997838

Strand: Direct

Name: miaE [H]

Synonym: VCM66_1834

Alternate gene names: 227082040

Gene position: 1997062-1997838 (Clockwise)

Preceding gene: 227082039

Following gene: 227082054

Centisome position: 69.04

GC content: 43.11

Gene sequence:

>777_bases
GTGCCCATGATTCATCCATCTGCTATCCAACAACTTTTAGAACCGATTAACCATTTCCTGCAATGTCCTACACCCGACGA
ATGGGTTGAAGAAGCGCGCAAACCTGAAAACCTACGTGTTTTGTTGTTAGACCATTTACTATGTGAGCTGAAAGCTGGTC
AATCCGCCATGTTTCTGATCCGTAAGTATGCGGTTGATAAAGAGAGTGAACTTTCGCTATTGGATTGGTTTAAACCTTAT
GAAGACTTTGCGTATCGCAAAATTGGAGATATCCAATCGCTCAAAGGCAAAAGCCAAATCTCCAAAGCGATCATTGCCAA
ATCGGATTCTCCTTATAGCCAAGATTTGATCGATAAAATGGTATTGCTAATCAAGGAAGAGCTACACCATTTCTATCAAG
TGTTGGAAATTATGCAAGCGCGCAATGTGGAGTATGAGTCAATTCCGGCTAGCCGTTATGCCAAAAGCCTTCTAGCCCAT
ATGAAGACTCATGAACCACAGACATTGATTGATAAGCTGATAGTAGGCGCTTATATTGAGGCTCGCTCTTGTGAGCGCTT
TGCCAAACTCGCCCCCTACTTAGATGACGAGATAGCAAAATTTTATGTTTCTCTGCTTCGCTCTGAAGCTCGCCATTATC
AAGACTACTTGCAACTGGCTGAACAAATTGCGGGCTACGATATCTCTGAACGTGTTGCGTATTTTGGCCGAGTCGAAGCG
GAGTTAATTTCCACTCCCGATAGCGATTTCAAGTTTCACAGTGGTATTCCTAAATAG

Upstream 100 bases:

>100_bases
GACATGGATGTGCTCTTTGGCGAGAAGAAAAAGTGGTAAGATACCGCACGATAATGACTTGATTAACTTCAGGCCGCTTA
TGTGGCCTTTTTCTTAAATA

Downstream 100 bases:

>100_bases
CCTTTTTAAAAAAGCCCAGTACTGTGACTGGGCTTGAGTTTATCCAATCTAGTAAAGCTAAACTAAAGAGCTGTTTATCT
CTTCCAGAACCACTTCAGGA

Product: tRNA-(MS[2]IO[6]A)-hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MPMIHPSAIQQLLEPINHFLQCPTPDEWVEEARKPENLRVLLLDHLLCELKAGQSAMFLIRKYAVDKESELSLLDWFKPY
EDFAYRKIGDIQSLKGKSQISKAIIAKSDSPYSQDLIDKMVLLIKEELHHFYQVLEIMQARNVEYESIPASRYAKSLLAH
MKTHEPQTLIDKLIVGAYIEARSCERFAKLAPYLDDEIAKFYVSLLRSEARHYQDYLQLAEQIAGYDISERVAYFGRVEA
ELISTPDSDFKFHSGIPK

Sequences:

>Translated_258_residues
MPMIHPSAIQQLLEPINHFLQCPTPDEWVEEARKPENLRVLLLDHLLCELKAGQSAMFLIRKYAVDKESELSLLDWFKPY
EDFAYRKIGDIQSLKGKSQISKAIIAKSDSPYSQDLIDKMVLLIKEELHHFYQVLEIMQARNVEYESIPASRYAKSLLAH
MKTHEPQTLIDKLIVGAYIEARSCERFAKLAPYLDDEIAKFYVSLLRSEARHYQDYLQLAEQIAGYDISERVAYFGRVEA
ELISTPDSDFKFHSGIPK
>Mature_257_residues
PMIHPSAIQQLLEPINHFLQCPTPDEWVEEARKPENLRVLLLDHLLCELKAGQSAMFLIRKYAVDKESELSLLDWFKPYE
DFAYRKIGDIQSLKGKSQISKAIIAKSDSPYSQDLIDKMVLLIKEELHHFYQVLEIMQARNVEYESIPASRYAKSLLAHM
KTHEPQTLIDKLIVGAYIEARSCERFAKLAPYLDDEIAKFYVSLLRSEARHYQDYLQLAEQIAGYDISERVAYFGRVEAE
LISTPDSDFKFHSGIPK

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 29854; Mature: 29722

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMIHPSAIQQLLEPINHFLQCPTPDEWVEEARKPENLRVLLLDHLLCELKAGQSAMFLI
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHH
RKYAVDKESELSLLDWFKPYEDFAYRKIGDIQSLKGKSQISKAIIAKSDSPYSQDLIDKM
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHH
VLLIKEELHHFYQVLEIMQARNVEYESIPASRYAKSLLAHMKTHEPQTLIDKLIVGAYIE
HHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ARSCERFAKLAPYLDDEIAKFYVSLLRSEARHYQDYLQLAEQIAGYDISERVAYFGRVEA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ELISTPDSDFKFHSGIPK
HHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PMIHPSAIQQLLEPINHFLQCPTPDEWVEEARKPENLRVLLLDHLLCELKAGQSAMFLI
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHH
RKYAVDKESELSLLDWFKPYEDFAYRKIGDIQSLKGKSQISKAIIAKSDSPYSQDLIDKM
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHH
VLLIKEELHHFYQVLEIMQARNVEYESIPASRYAKSLLAHMKTHEPQTLIDKLIVGAYIE
HHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ARSCERFAKLAPYLDDEIAKFYVSLLRSEARHYQDYLQLAEQIAGYDISERVAYFGRVEA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ELISTPDSDFKFHSGIPK
HHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]