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The map label for this gene is hlyB [H]

Identifier: 227082028

GI number: 227082028

Start: 1981511

End: 1983529

Strand: Direct

Name: hlyB [H]

Synonym: VCM66_1822

Alternate gene names: 227082028

Gene position: 1981511-1983529 (Clockwise)

Preceding gene: 227082021

Following gene: 227082029

Centisome position: 68.5

GC content: 48.59

Gene sequence:

>2019_bases
ATGAGTGATTTTCGAGGGAGAGAAATTATGCAGGGTTCCGTTATTCGGCGTATGTATGCCGGTTTTGCGCTGATCATCAT
AATGTTTGTGGTGACTGTCGCCATCATGCTTCGTGGCATGAATCAAATTCACAGCAATTTTGAAAGTGTTTCAACCGCTG
CATTGCCTCTCGTCTCTCTCTCTAATCAGACGAGTGTTCAACTCTTGTCTGCCGATAAATCGTTTAAAGACTTTCTGACT
ACGCAAGATACTTCGCTCATGGACGGTATGCGTCAAGAGTTTGCTCAGTCTAAACAGCGCTTTCAATCTGTTTTGGCGGA
ACTGAGTCAAGCCAGTGTGAATCATCCCGAACTGGCACAGCCAATCACGGAATTAAAGCAGCTCGAAACCCGCTATTTTA
GTGAAGCTGACCTCGCCATGGATAACTATCAAGCCATGTTTGCTGCTCAAGCTTCCGTGCAAAAGTCGACTCGTGAGTTC
CAACGTCTTCATTCAGAACTGAGTGTCGGGATGAAAGAGTTTGTCGATGACAACGACAGCATTTCGGTCAAAGTGATGGC
CAAAAGCTACTTCATCAAATTAAAAGATGCCGAAGTGATCACTTCTGATGCCTTAGCCAGTTCAGATGTCGCCTTTGTGG
AAAAAGCGGTCAATCAAAACAAAAAAGCGGTTACCCACCTAAACTACGCGTACCGAGGCCTCACCACGCAGATGCCGCAG
TTGAAAGAGCGCTTTGATGAGCCAGTCGCGAAATTCACCCAAGACATCGGCCAAAAAGGCGGCGTACTGGATCAACACAA
TGAATATCTGCAAGCTCGCGCTGCGCTCTATAACAATATTGCCAACCTCGCCAATGAAGTGGACAAAGCAATGACCATTT
TAGGCGCGTTTAACCAAACGGCGACCGACGACCTGAACCAATCCCTTGCTGAAGCCGGCTCAGTTTACCAACAAGGTGCG
ATTAAAGCGGTAGTGATTGGTATTGCCGTCGTTCTATTGGCAACTGCGATTGGTTACCACATTGCGCACAGTGTGCGTGA
ACCCGTAACTCGGATCCTCAAAACCTTAGAAAGCCTCACCCAAGGCAATATGACGCAACGAATTGAGATTCGCTACAACA
ACGAATTCAGCCGCCTGAGCCGTCACATCAATAGTCTTGCCGATAACCTACACGACATTTTAGTCAAGCTCAACAGTGCC
TCCGATGATTTGACCGCGACAGCATCGACAAACCAAACGACCTCACTGAGTGCACAAAACAAGCTCAACAGTCAACGTGA
ACAGACCTCTAGCGTCGCAGCCGCAATGACGGAAATGGCTCACTCGGTGCAAGAAGTCGCCAATAGTGCGCAAAATTCAC
TGGCGATGGTGCAACAAGTCGAAACCGCATCGGAGTCAGGCCGCCAGATCATGAGCACCAACATCAGCACAATCCGTCAA
CTTGAAACACGCTTAATCGAGTCAGTCGAGGCGGTCGGCGACTTACGCAAGATGAGCAGCCAAATCGGCTCTATCTTAGA
TGTCATCCGCAATATTGCTGAGCAGACCAACCTTTTGGCGCTCAATGCTGCGATTGAAGCGGCGCGTGCAGGAGAACAAG
GCCGCGGCTTTGCGGTGGTGGCGGATGAAGTTCGCGTTCTTGCGCAGCGCACAACGCAATCGACGTCAGAAATCGAATCG
ATGATCAGCAATCTGCAATCCAGCTCTTCATTAGCCAGTAAGGTTATTGAGAGCTGTATGCAAGATATGGAACTCTCCGT
CGAACAAGCTTCCCATGCCAACAGTGCCATGGAAGAAATTCAAGCATTGATCATCGAAATCAGCCATATGAGTACGCATA
TCTCTCAAGCGGCCGGTGAGCAAAACGAAACCACCACCTCGATCGCTCGCAGCATCGAAGATATCAACCATATCTCGGAT
GAAAGCTACCAAGCGATGTCACAAATTGCGCAAACCAGTGCGAATTTGACCGCACTCGCCAACCAACAGAGTGAGTTAGT
CCATAGGTTTAAACTCTAA

Upstream 100 bases:

>100_bases
TGCTGTATTGGTCATAAAACGCGTTTCCCAACCTCAATACAATAATGACTGTTTTGTTACTTTTTTAAGACAAATTTGTC
TTTGATGAATTCTAATAACA

Downstream 100 bases:

>100_bases
TCCGATGAACGGCTAGCACTCTTGCTAGATACTCTTACTGTCAATTTTTCCACAAAGAGCGACTTTTCATCAGAGTCGCT
CTTGTTTTTTCAACACCTCA

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 672; Mature: 671

Protein sequence:

>672_residues
MSDFRGREIMQGSVIRRMYAGFALIIIMFVVTVAIMLRGMNQIHSNFESVSTAALPLVSLSNQTSVQLLSADKSFKDFLT
TQDTSLMDGMRQEFAQSKQRFQSVLAELSQASVNHPELAQPITELKQLETRYFSEADLAMDNYQAMFAAQASVQKSTREF
QRLHSELSVGMKEFVDDNDSISVKVMAKSYFIKLKDAEVITSDALASSDVAFVEKAVNQNKKAVTHLNYAYRGLTTQMPQ
LKERFDEPVAKFTQDIGQKGGVLDQHNEYLQARAALYNNIANLANEVDKAMTILGAFNQTATDDLNQSLAEAGSVYQQGA
IKAVVIGIAVVLLATAIGYHIAHSVREPVTRILKTLESLTQGNMTQRIEIRYNNEFSRLSRHINSLADNLHDILVKLNSA
SDDLTATASTNQTTSLSAQNKLNSQREQTSSVAAAMTEMAHSVQEVANSAQNSLAMVQQVETASESGRQIMSTNISTIRQ
LETRLIESVEAVGDLRKMSSQIGSILDVIRNIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRVLAQRTTQSTSEIES
MISNLQSSSSLASKVIESCMQDMELSVEQASHANSAMEEIQALIIEISHMSTHISQAAGEQNETTTSIARSIEDINHISD
ESYQAMSQIAQTSANLTALANQQSELVHRFKL

Sequences:

>Translated_672_residues
MSDFRGREIMQGSVIRRMYAGFALIIIMFVVTVAIMLRGMNQIHSNFESVSTAALPLVSLSNQTSVQLLSADKSFKDFLT
TQDTSLMDGMRQEFAQSKQRFQSVLAELSQASVNHPELAQPITELKQLETRYFSEADLAMDNYQAMFAAQASVQKSTREF
QRLHSELSVGMKEFVDDNDSISVKVMAKSYFIKLKDAEVITSDALASSDVAFVEKAVNQNKKAVTHLNYAYRGLTTQMPQ
LKERFDEPVAKFTQDIGQKGGVLDQHNEYLQARAALYNNIANLANEVDKAMTILGAFNQTATDDLNQSLAEAGSVYQQGA
IKAVVIGIAVVLLATAIGYHIAHSVREPVTRILKTLESLTQGNMTQRIEIRYNNEFSRLSRHINSLADNLHDILVKLNSA
SDDLTATASTNQTTSLSAQNKLNSQREQTSSVAAAMTEMAHSVQEVANSAQNSLAMVQQVETASESGRQIMSTNISTIRQ
LETRLIESVEAVGDLRKMSSQIGSILDVIRNIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRVLAQRTTQSTSEIES
MISNLQSSSSLASKVIESCMQDMELSVEQASHANSAMEEIQALIIEISHMSTHISQAAGEQNETTTSIARSIEDINHISD
ESYQAMSQIAQTSANLTALANQQSELVHRFKL
>Mature_671_residues
SDFRGREIMQGSVIRRMYAGFALIIIMFVVTVAIMLRGMNQIHSNFESVSTAALPLVSLSNQTSVQLLSADKSFKDFLTT
QDTSLMDGMRQEFAQSKQRFQSVLAELSQASVNHPELAQPITELKQLETRYFSEADLAMDNYQAMFAAQASVQKSTREFQ
RLHSELSVGMKEFVDDNDSISVKVMAKSYFIKLKDAEVITSDALASSDVAFVEKAVNQNKKAVTHLNYAYRGLTTQMPQL
KERFDEPVAKFTQDIGQKGGVLDQHNEYLQARAALYNNIANLANEVDKAMTILGAFNQTATDDLNQSLAEAGSVYQQGAI
KAVVIGIAVVLLATAIGYHIAHSVREPVTRILKTLESLTQGNMTQRIEIRYNNEFSRLSRHINSLADNLHDILVKLNSAS
DDLTATASTNQTTSLSAQNKLNSQREQTSSVAAAMTEMAHSVQEVANSAQNSLAMVQQVETASESGRQIMSTNISTIRQL
ETRLIESVEAVGDLRKMSSQIGSILDVIRNIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRVLAQRTTQSTSEIESM
ISNLQSSSSLASKVIESCMQDMELSVEQASHANSAMEEIQALIIEISHMSTHISQAAGEQNETTTSIARSIEDINHISDE
SYQAMSQIAQTSANLTALANQQSELVHRFKL

Specific function: May form a pore through which the hemolysin can be exported [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=325, Percent_Identity=27.3846153846154, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1787690, Length=453, Percent_Identity=26.0485651214128, Blast_Score=126, Evalue=6e-30,
Organism=Escherichia coli, GI1788195, Length=292, Percent_Identity=29.7945205479452, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1788194, Length=314, Percent_Identity=27.7070063694268, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI1789453, Length=239, Percent_Identity=31.3807531380753, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660
- InterPro:   IPR000727 [H]

Pfam domain/function: PF00672 HAMP; PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 73745; Mature: 73614

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2 ; PS50192 T_SNARE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCC
>Mature Secondary Structure 
SDFRGREIMQGSVIRRMYAGFALIIIMFVVTVAIMLRGMNQIHSNFESVSTAALPLVSL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SNQTSVQLLSADKSFKDFLTTQDTSLMDGMRQEFAQSKQRFQSVLAELSQASVNHPELAQ
CCCCHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
PITELKQLETRYFSEADLAMDNYQAMFAAQASVQKSTREFQRLHSELSVGMKEFVDDNDS
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ISVKVMAKSYFIKLKDAEVITSDALASSDVAFVEKAVNQNKKAVTHLNYAYRGLTTQMPQ
EEEEEEEHHHEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHH
LKERFDEPVAKFTQDIGQKGGVLDQHNEYLQARAALYNNIANLANEVDKAMTILGAFNQT
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
ATDDLNQSLAEAGSVYQQGAIKAVVIGIAVVLLATAIGYHIAHSVREPVTRILKTLESLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGNMTQRIEIRYNNEFSRLSRHINSLADNLHDILVKLNSASDDLTATASTNQTTSLSAQN
CCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHH
KLNSQREQTSSVAAAMTEMAHSVQEVANSAQNSLAMVQQVETASESGRQIMSTNISTIRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LETRLIESVEAVGDLRKMSSQIGSILDVIRNIAEQTNLLALNAAIEAARAGEQGRGFAVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEH
ADEVRVLAQRTTQSTSEIESMISNLQSSSSLASKVIESCMQDMELSVEQASHANSAMEEI
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QALIIEISHMSTHISQAAGEQNETTTSIARSIEDINHISDESYQAMSQIAQTSANLTALA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHH
NQQSELVHRFKL
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2162464; 10952301 [H]