Click here to switch to the map view.

The map label for this gene is pflB [H]

Identifier: 227081995

GI number: 227081995

Start: 1943262

End: 1945625

Strand: Direct

Name: pflB [H]

Synonym: VCM66_1789

Alternate gene names: 227081995

Gene position: 1943262-1945625 (Clockwise)

Preceding gene: 227081994

Following gene: 227081996

Centisome position: 67.18

GC content: 47.93

Gene sequence:

>2364_bases
TTGAAGAGTGTACTGCTCCCTTCGGGGATAGCTGGCTCAACGGCGAAAACAGTACGTGTTTTTTCTACTCATAAAGGTAG
GTATGTCATGGCAGAGCAATTTGCTAAAGCTTGGGAAGGTTTTGCGGCAGGTGACTGGCAAAACGAAGTTAACGTTCGTG
ATTTTATTCAAAAGAACTACACACCGTACGAAGGCGACGAATCTTTCCTAGTCTCAGAAGGTACTGAAGCGACCAACAAG
CTTTGGGCTAAAGTAATGGAAGGTATCAGACAGGAAAACTCAACTCACGCTCCTGTTGACTTCGACACTTCTCTTATCTC
TACCATCACAGCTCACGACGCTGGCTACATCAACAAAGATCTTGAAAAGATCGTTGGTCTACAAACCGATGCTCCACTGA
AACGTGCAATCATCCCTAATGGTGGTATCCGTATGGTGGAAGGTTCATGCAAAGCATACGGTCGTGAACTGGATCCACAA
GTTTCAAAAATCTACTCTGAATACCGCAAAACGCACAACGCCGGTGTATTCGACATCTACACTCCAGAAATTCTGGCTTG
TCGTAAATCTGGCGTTCTGACTGGTCTTCCAGATGCTTACGGCCGTGGTCGTATCATCGGTGACTACCGTCGTGTAGCGT
TGTACGGTATCGACTTCCTGATGAAAGACAAACTGGCTCAGTTCAAGTCTCTGCAGGAAAAATTTGAAAACGGCGAAGAT
CTGCAAATGACCATGCAACTGCGTGAAGAAATTGCAGAACAACACCGTGCGCTTGGCCAAATGAAACAGATGGCTGCGAA
ATACGGTTACGATATTTCTCGCCCTGCTGAAACGGCACAAGAAGCAATCCAATGGACTTACTTCGGCTACCTAGCGGCAG
TGAAATCTCAAAACGGTGCTGCAATGTCTCTTGGCCGTACCTCTACTTTCTTGGACGTGTACATTGAGCGCGACATGAAA
GCCGGCAAGATCACTGAAGTAGAAGCTCAGGAAATGATCGACCACTTCGTCATGAAGCTGCGTATGGTTCGTTTCCTACG
TACTCCTGAGTACGATGAGCTGTTTTCTGGTGACCCAATTTGGGCAACCGAATCTATGGGTGGTATGGGTCTAGACGGTC
GTACGCTGGTAACACGTACTAACTTCCGCTTCCTGAACTCACTGTACACCATGGGTCCATCTCCAGAGCCAAACATCACT
GTCCTGTGGTCAGAGCAACTGCCTGAAGGCTTCAAGAAGTTCTGTGCGAAAGTGTCTATCGATACCTCGTCTATCCAGTA
CGAAAACGATGACTTGATGCGTCCTGACTTCAACAATGATGACTACGCTATCGCATGTTGTGTATCACCAATGGTTATTG
GTAAACACATGCAGTTCTTCGGTGCTCGCGCTAACCTTGCTAAAACGCTGCTGTATGTAATCAACGGTGGTGTGGATGAG
AAACTGAAAATCCAAGTGGGTCCAAAAATGCCTAAGATCACCGACGAAGTGCTCGACTTCGACGATGTATGGGGCAAGCT
GGATCACTTCATGGGTTGGCTGGCAACACAATACGTCACTGCACTGAATGCCATCCACTACATGCATGACAAGTACAGCT
ACGAAGCGGCTCTAATGGCGCTGCATGACCGTGATGTACGTCGTACTATGGCGTGTGGTATCGCAGGTCTGTCTGTAGCG
GCTGACTCACTGTCTGCTATCAAGTACGCGAAAGTAAAACCAATTCGTGACGAAGATGGTGTAGCTATCGATTTTGAAAT
TGAAGGCGACTATCCAAAATTCGGTAACAACGATTCACGCGTTGATGACATCGCCTGTGAACTGGTTGAGCGTTTCATGA
ACAAGATCCGTTCACTGAAAACTTACCGTAACGCAGTACCAACTCAGTCTATCCTGACTATCACGTCAAACGTGGTATAC
GGCAAGAAGACAGGTAACACGCCAGACGGTCGCCGTGCAGGTGCGCCATTTGCACCGGGTGCAAACCCAATGCACGGCCG
TGACGAGAAAGGGGCGGTAGCGTCTCTGACTTCCGTCGGTAAACTGCCATTTGCTCACGCGAAAGACGGTATCTCTTATA
CCTTCTCTATCGTGCCAAATGCACTGGGTAAAGATGAGAACTCACAGCGTGCGAACCTAGCTGGCTTGATGGACGGTTAC
TTCCACCACGAAGCAGGCATCGAGGGTGGTCAACACCTGAACGTTAACGTTCTGAACCGTGACACTCTGCTTGATGCAGT
TAAGCATCCAGAGAAGTACCCACAACTGACTATCCGTGTATCTGGATATGCAGTGCGCTTTAACTCTCTGACTGCTGAAC
AACAACAAGACGTTATTGCTCGTACTTTCACTGAATCTCTATAA

Upstream 100 bases:

>100_bases
TAAGGTTTACGAAAAATTTTTAGCGTAGAGTGGCTCCATTAAAGGAACTAATAGCGATATCCATATTCAAATAGTCACAG
TGTGAATTTGGATATCGTTA

Downstream 100 bases:

>100_bases
GATTCAGTAAGCCTCTTCAAAGCCCCGTTTCGACGGGGCTTTTTGTTTTTCCGCGCAACAGGATTTATTTCTGTATCTCA
CGACACATTTGTGTTCTTCT

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 1 [H]

Number of amino acids: Translated: 787; Mature: 787

Protein sequence:

>787_residues
MKSVLLPSGIAGSTAKTVRVFSTHKGRYVMAEQFAKAWEGFAAGDWQNEVNVRDFIQKNYTPYEGDESFLVSEGTEATNK
LWAKVMEGIRQENSTHAPVDFDTSLISTITAHDAGYINKDLEKIVGLQTDAPLKRAIIPNGGIRMVEGSCKAYGRELDPQ
VSKIYSEYRKTHNAGVFDIYTPEILACRKSGVLTGLPDAYGRGRIIGDYRRVALYGIDFLMKDKLAQFKSLQEKFENGED
LQMTMQLREEIAEQHRALGQMKQMAAKYGYDISRPAETAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDVYIERDMK
AGKITEVEAQEMIDHFVMKLRMVRFLRTPEYDELFSGDPIWATESMGGMGLDGRTLVTRTNFRFLNSLYTMGPSPEPNIT
VLWSEQLPEGFKKFCAKVSIDTSSIQYENDDLMRPDFNNDDYAIACCVSPMVIGKHMQFFGARANLAKTLLYVINGGVDE
KLKIQVGPKMPKITDEVLDFDDVWGKLDHFMGWLATQYVTALNAIHYMHDKYSYEAALMALHDRDVRRTMACGIAGLSVA
ADSLSAIKYAKVKPIRDEDGVAIDFEIEGDYPKFGNNDSRVDDIACELVERFMNKIRSLKTYRNAVPTQSILTITSNVVY
GKKTGNTPDGRRAGAPFAPGANPMHGRDEKGAVASLTSVGKLPFAHAKDGISYTFSIVPNALGKDENSQRANLAGLMDGY
FHHEAGIEGGQHLNVNVLNRDTLLDAVKHPEKYPQLTIRVSGYAVRFNSLTAEQQQDVIARTFTESL

Sequences:

>Translated_787_residues
MKSVLLPSGIAGSTAKTVRVFSTHKGRYVMAEQFAKAWEGFAAGDWQNEVNVRDFIQKNYTPYEGDESFLVSEGTEATNK
LWAKVMEGIRQENSTHAPVDFDTSLISTITAHDAGYINKDLEKIVGLQTDAPLKRAIIPNGGIRMVEGSCKAYGRELDPQ
VSKIYSEYRKTHNAGVFDIYTPEILACRKSGVLTGLPDAYGRGRIIGDYRRVALYGIDFLMKDKLAQFKSLQEKFENGED
LQMTMQLREEIAEQHRALGQMKQMAAKYGYDISRPAETAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDVYIERDMK
AGKITEVEAQEMIDHFVMKLRMVRFLRTPEYDELFSGDPIWATESMGGMGLDGRTLVTRTNFRFLNSLYTMGPSPEPNIT
VLWSEQLPEGFKKFCAKVSIDTSSIQYENDDLMRPDFNNDDYAIACCVSPMVIGKHMQFFGARANLAKTLLYVINGGVDE
KLKIQVGPKMPKITDEVLDFDDVWGKLDHFMGWLATQYVTALNAIHYMHDKYSYEAALMALHDRDVRRTMACGIAGLSVA
ADSLSAIKYAKVKPIRDEDGVAIDFEIEGDYPKFGNNDSRVDDIACELVERFMNKIRSLKTYRNAVPTQSILTITSNVVY
GKKTGNTPDGRRAGAPFAPGANPMHGRDEKGAVASLTSVGKLPFAHAKDGISYTFSIVPNALGKDENSQRANLAGLMDGY
FHHEAGIEGGQHLNVNVLNRDTLLDAVKHPEKYPQLTIRVSGYAVRFNSLTAEQQQDVIARTFTESL
>Mature_787_residues
MKSVLLPSGIAGSTAKTVRVFSTHKGRYVMAEQFAKAWEGFAAGDWQNEVNVRDFIQKNYTPYEGDESFLVSEGTEATNK
LWAKVMEGIRQENSTHAPVDFDTSLISTITAHDAGYINKDLEKIVGLQTDAPLKRAIIPNGGIRMVEGSCKAYGRELDPQ
VSKIYSEYRKTHNAGVFDIYTPEILACRKSGVLTGLPDAYGRGRIIGDYRRVALYGIDFLMKDKLAQFKSLQEKFENGED
LQMTMQLREEIAEQHRALGQMKQMAAKYGYDISRPAETAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDVYIERDMK
AGKITEVEAQEMIDHFVMKLRMVRFLRTPEYDELFSGDPIWATESMGGMGLDGRTLVTRTNFRFLNSLYTMGPSPEPNIT
VLWSEQLPEGFKKFCAKVSIDTSSIQYENDDLMRPDFNNDDYAIACCVSPMVIGKHMQFFGARANLAKTLLYVINGGVDE
KLKIQVGPKMPKITDEVLDFDDVWGKLDHFMGWLATQYVTALNAIHYMHDKYSYEAALMALHDRDVRRTMACGIAGLSVA
ADSLSAIKYAKVKPIRDEDGVAIDFEIEGDYPKFGNNDSRVDDIACELVERFMNKIRSLKTYRNAVPTQSILTITSNVVY
GKKTGNTPDGRRAGAPFAPGANPMHGRDEKGAVASLTSVGKLPFAHAKDGISYTFSIVPNALGKDENSQRANLAGLMDGY
FHHEAGIEGGQHLNVNVLNRDTLLDAVKHPEKYPQLTIRVSGYAVRFNSLTAEQQQDVIARTFTESL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=758, Percent_Identity=82.8496042216359, Blast_Score=1347, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=754, Percent_Identity=75.4641909814324, Blast_Score=1238, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=581, Percent_Identity=27.3666092943201, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1790388, Length=656, Percent_Identity=23.3231707317073, Blast_Score=136, Evalue=6e-33,
Organism=Escherichia coli, GI1788933, Length=64, Percent_Identity=87.5, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 87785; Mature: 87785

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVLLPSGIAGSTAKTVRVFSTHKGRYVMAEQFAKAWEGFAAGDWQNEVNVRDFIQKNY
CCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCC
TPYEGDESFLVSEGTEATNKLWAKVMEGIRQENSTHAPVDFDTSLISTITAHDAGYINKD
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHH
LEKIVGLQTDAPLKRAIIPNGGIRMVEGSCKAYGRELDPQVSKIYSEYRKTHNAGVFDIY
HHHHHCCCCCCCHHHHCCCCCCEEEEECCHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEC
TPEILACRKSGVLTGLPDAYGRGRIIGDYRRVALYGIDFLMKDKLAQFKSLQEKFENGED
CCHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LQMTMQLREEIAEQHRALGQMKQMAAKYGYDISRPAETAQEAIQWTYFGYLAAVKSQNGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
AMSLGRTSTFLDVYIERDMKAGKITEVEAQEMIDHFVMKLRMVRFLRTPEYDELFSGDPI
EEECCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCC
WATESMGGMGLDGRTLVTRTNFRFLNSLYTMGPSPEPNITVLWSEQLPEGFKKFCAKVSI
EEECCCCCCCCCCCEEEEECHHHHHHHHHHCCCCCCCCEEEEECHHCHHHHHHHHHEEEC
DTSSIQYENDDLMRPDFNNDDYAIACCVSPMVIGKHMQFFGARANLAKTLLYVINGGVDE
CCCCEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KLKIQVGPKMPKITDEVLDFDDVWGKLDHFMGWLATQYVTALNAIHYMHDKYSYEAALMA
EEEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHDRDVRRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGVAIDFEIEGDYPKFGNNDSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCH
VDDIACELVERFMNKIRSLKTYRNAVPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEECCCEEEECCCCCCCCCCCCCCCCCCC
ANPMHGRDEKGAVASLTSVGKLPFAHAKDGISYTFSIVPNALGKDENSQRANLAGLMDGY
CCCCCCCCCCCHHHHHHHHCCCCCHHCCCCCEEEEEECCHHCCCCCCCCHHHHHHHHHHH
FHHEAGIEGGQHLNVNVLNRDTLLDAVKHPEKYPQLTIRVSGYAVRFNSLTAEQQQDVIA
HHHHCCCCCCCEEEEEEECHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHH
RTFTESL
HHHHHCC
>Mature Secondary Structure
MKSVLLPSGIAGSTAKTVRVFSTHKGRYVMAEQFAKAWEGFAAGDWQNEVNVRDFIQKNY
CCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCC
TPYEGDESFLVSEGTEATNKLWAKVMEGIRQENSTHAPVDFDTSLISTITAHDAGYINKD
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHH
LEKIVGLQTDAPLKRAIIPNGGIRMVEGSCKAYGRELDPQVSKIYSEYRKTHNAGVFDIY
HHHHHCCCCCCCHHHHCCCCCCEEEEECCHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEC
TPEILACRKSGVLTGLPDAYGRGRIIGDYRRVALYGIDFLMKDKLAQFKSLQEKFENGED
CCHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LQMTMQLREEIAEQHRALGQMKQMAAKYGYDISRPAETAQEAIQWTYFGYLAAVKSQNGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
AMSLGRTSTFLDVYIERDMKAGKITEVEAQEMIDHFVMKLRMVRFLRTPEYDELFSGDPI
EEECCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCC
WATESMGGMGLDGRTLVTRTNFRFLNSLYTMGPSPEPNITVLWSEQLPEGFKKFCAKVSI
EEECCCCCCCCCCCEEEEECHHHHHHHHHHCCCCCCCCEEEEECHHCHHHHHHHHHEEEC
DTSSIQYENDDLMRPDFNNDDYAIACCVSPMVIGKHMQFFGARANLAKTLLYVINGGVDE
CCCCEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KLKIQVGPKMPKITDEVLDFDDVWGKLDHFMGWLATQYVTALNAIHYMHDKYSYEAALMA
EEEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHDRDVRRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGVAIDFEIEGDYPKFGNNDSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCH
VDDIACELVERFMNKIRSLKTYRNAVPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEECCCEEEECCCCCCCCCCCCCCCCCCC
ANPMHGRDEKGAVASLTSVGKLPFAHAKDGISYTFSIVPNALGKDENSQRANLAGLMDGY
CCCCCCCCCCCHHHHHHHHCCCCCHHCCCCCEEEEEECCHHCCCCCCCCHHHHHHHHHHH
FHHEAGIEGGQHLNVNVLNRDTLLDAVKHPEKYPQLTIRVSGYAVRFNSLTAEQQQDVIA
HHHHCCCCCCCEEEEEEECHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHH
RTFTESL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3053170; 8905232; 9278503; 2651404; 1310545; 9629924; 10504733 [H]