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The map label for this gene is tolB

Identifier: 227081965

GI number: 227081965

Start: 1913510

End: 1914862

Strand: Reverse

Name: tolB

Synonym: VCM66_1759

Alternate gene names: 227081965

Gene position: 1914862-1913510 (Counterclockwise)

Preceding gene: 227081966

Following gene: 227081964

Centisome position: 66.2

GC content: 45.38

Gene sequence:

>1353_bases
GTGTTTAAGCGATTAGTTTTTGTTTTGATGCTCATCGGTACCGGTTTTTCCAATATTGCGAATGCAGCATTGGAGCTAGT
TATTACTGACGGTATTGATTCTGCACGTCCGATTGCGATAGTTCCTTTCAAATGGGAAGGCGCAACCAAGTTGCCTGAAG
ACGTTTCTGCGGTGATTGCCTCTGACTTACAGCGCAGTGGTAAATTTAGCCCAGTTCCGACCAGCAAAATGCCACAGACG
CCTTATAGCGAAGAGCAGGTTAACTTTGGCAAATGGACATCTATGGGGGTCGATTCATTATTGACGGGTACGATCACGCA
AAATGCGGAAGGTAGCTATGTAATTAGTTATCAACTGGTTGACATTGTGCGTGGACAACTGACTCAAGGCCAAAGCAAAG
CTCTGTCTCAAGATGGTCAATTGGTGCTATCTAAAGATCATGTTTTGTTTAATAAAGTGGCGACCGTTCCTGCATCAAGA
ATGCGTGAATATGCCCACCGTATTGCGGACTTGGTTTATGAGGAATTGACCGGTGAACGCGGCGCCTTCTTAACGCGCAT
CGCTTACGTTGTGGTCAATGATAAGGATCCGTATCCTTATCAACTGAGAATTGCTGATTATGACGGCTATAACGAACGTC
TTGTACTTCGTTCTAAGCAGCCGCTTATGTCACCTGCTTGGTCACCTGATGGTCAGACTTTGGCTTACGTGAGTTTCCAA
AACGGACAAGCCGAAATTTACATGATGAACATCTACAGTGGGAAGCGAGAGAAGCTGACGTCATTCCCACGTCATAATGG
TGCACCCAGATTCTCACCAGATGGTAAAACGCTAGCGCTGGTTTTATCAAAAACTGGTAATTTACAGGTCTACACGATGG
ACTTGGCTACACGTCGCTTAACAGAGGTGACTAGTGGCCGTTCCAACAACACCGAACCTTTCTGGCATCCAGATGGTAAA
TCTTTAATTTTCACTTCAGATCGGGGTGGTAAACCACAGATTTATCAAGTAAATTTATCCGGCGGTGAAACCAAGCGTTT
AACTTGGCAAGGTAGCCAAAACTTGGGTGGCCAAATTACACCGGATGGAAAATTCTTGGTGATGGTGAATCGTAGTGATT
CCGGCTTCAATCTCGCGAAGCAGGATTTAGAAACAGGGGCAATGCAGATTCTGACAAAAACCTTGTTAGATGAGTCCCCA
AGTATTGCGCCGAATGGTGGTATGGTGATTTATAGCTCCATCTACAATAAGGCCAACGTCTTGTCGATGGTATCCATTGA
TGGACGCTTTAAAGCAAGATTACCGGCAACCAATGGGCGCGTTCGTGCTCCAGCATGGTCACCGTTTTTATAA

Upstream 100 bases:

>100_bases
AAGTGAATAGTTTTCCATTGCCGAAAGATCAACCTGACGTTGTGGAAAAACTAAAGAATATTAATTTAACCGTAGCACCT
GAATAAGGAAGGGAAGGCTT

Downstream 100 bases:

>100_bases
CAAGTAACTGTTTAATAGAAAGGAATAGGAAATGCAACTTAACAAAGTTCTTAAGAGTCTGCTGATTGCGCTACCAGTTT
TAGCTGTTACCGCTTGTAGC

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MFKRLVFVLMLIGTGFSNIANAALELVITDGIDSARPIAIVPFKWEGATKLPEDVSAVIASDLQRSGKFSPVPTSKMPQT
PYSEEQVNFGKWTSMGVDSLLTGTITQNAEGSYVISYQLVDIVRGQLTQGQSKALSQDGQLVLSKDHVLFNKVATVPASR
MREYAHRIADLVYEELTGERGAFLTRIAYVVVNDKDPYPYQLRIADYDGYNERLVLRSKQPLMSPAWSPDGQTLAYVSFQ
NGQAEIYMMNIYSGKREKLTSFPRHNGAPRFSPDGKTLALVLSKTGNLQVYTMDLATRRLTEVTSGRSNNTEPFWHPDGK
SLIFTSDRGGKPQIYQVNLSGGETKRLTWQGSQNLGGQITPDGKFLVMVNRSDSGFNLAKQDLETGAMQILTKTLLDESP
SIAPNGGMVIYSSIYNKANVLSMVSIDGRFKARLPATNGRVRAPAWSPFL

Sequences:

>Translated_450_residues
MFKRLVFVLMLIGTGFSNIANAALELVITDGIDSARPIAIVPFKWEGATKLPEDVSAVIASDLQRSGKFSPVPTSKMPQT
PYSEEQVNFGKWTSMGVDSLLTGTITQNAEGSYVISYQLVDIVRGQLTQGQSKALSQDGQLVLSKDHVLFNKVATVPASR
MREYAHRIADLVYEELTGERGAFLTRIAYVVVNDKDPYPYQLRIADYDGYNERLVLRSKQPLMSPAWSPDGQTLAYVSFQ
NGQAEIYMMNIYSGKREKLTSFPRHNGAPRFSPDGKTLALVLSKTGNLQVYTMDLATRRLTEVTSGRSNNTEPFWHPDGK
SLIFTSDRGGKPQIYQVNLSGGETKRLTWQGSQNLGGQITPDGKFLVMVNRSDSGFNLAKQDLETGAMQILTKTLLDESP
SIAPNGGMVIYSSIYNKANVLSMVSIDGRFKARLPATNGRVRAPAWSPFL
>Mature_450_residues
MFKRLVFVLMLIGTGFSNIANAALELVITDGIDSARPIAIVPFKWEGATKLPEDVSAVIASDLQRSGKFSPVPTSKMPQT
PYSEEQVNFGKWTSMGVDSLLTGTITQNAEGSYVISYQLVDIVRGQLTQGQSKALSQDGQLVLSKDHVLFNKVATVPASR
MREYAHRIADLVYEELTGERGAFLTRIAYVVVNDKDPYPYQLRIADYDGYNERLVLRSKQPLMSPAWSPDGQTLAYVSFQ
NGQAEIYMMNIYSGKREKLTSFPRHNGAPRFSPDGKTLALVLSKTGNLQVYTMDLATRRLTEVTSGRSNNTEPFWHPDGK
SLIFTSDRGGKPQIYQVNLSGGETKRLTWQGSQNLGGQITPDGKFLVMVNRSDSGFNLAKQDLETGAMQILTKTLLDESP
SIAPNGGMVIYSSIYNKANVLSMVSIDGRFKARLPATNGRVRAPAWSPFL

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=447, Percent_Identity=48.3221476510067, Blast_Score=460, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_VIBC3 (A5F753)

Other databases:

- EMBL:   AF187269
- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F753
- SMR:   A5F753
- STRING:   A5F753
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1429
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- ProtClustDB:   PRK04792
- BioCyc:   VCHO345073:VC0395_A1429-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 49645; Mature: 49645

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRLVFVLMLIGTGFSNIANAALELVITDGIDSARPIAIVPFKWEGATKLPEDVSAVIA
CHHHHHHHHHHHHCCHHHHHHHEEEEEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHHH
SDLQRSGKFSPVPTSKMPQTPYSEEQVNFGKWTSMGVDSLLTGTITQNAEGSYVISYQLV
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCEEEEEEEE
DIVRGQLTQGQSKALSQDGQLVLSKDHVLFNKVATVPASRMREYAHRIADLVYEELTGER
HHHHHHCCCCHHHHHCCCCCEEEECCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
GAFLTRIAYVVVNDKDPYPYQLRIADYDGYNERLVLRSKQPLMSPAWSPDGQTLAYVSFQ
CCEEEEEEEEEEECCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEC
NGQAEIYMMNIYSGKREKLTSFPRHNGAPRFSPDGKTLALVLSKTGNLQVYTMDLATRRL
CCCEEEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECHHHHHH
TEVTSGRSNNTEPFWHPDGKSLIFTSDRGGKPQIYQVNLSGGETKRLTWQGSQNLGGQIT
HHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEC
PDGKFLVMVNRSDSGFNLAKQDLETGAMQILTKTLLDESPSIAPNGGMVIYSSIYNKANV
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCE
LSMVSIDGRFKARLPATNGRVRAPAWSPFL
EEEEEECCEEEEECCCCCCEEECCCCCCCC
>Mature Secondary Structure
MFKRLVFVLMLIGTGFSNIANAALELVITDGIDSARPIAIVPFKWEGATKLPEDVSAVIA
CHHHHHHHHHHHHCCHHHHHHHEEEEEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHHH
SDLQRSGKFSPVPTSKMPQTPYSEEQVNFGKWTSMGVDSLLTGTITQNAEGSYVISYQLV
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCEEEEEEEE
DIVRGQLTQGQSKALSQDGQLVLSKDHVLFNKVATVPASRMREYAHRIADLVYEELTGER
HHHHHHCCCCHHHHHCCCCCEEEECCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
GAFLTRIAYVVVNDKDPYPYQLRIADYDGYNERLVLRSKQPLMSPAWSPDGQTLAYVSFQ
CCEEEEEEEEEEECCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEC
NGQAEIYMMNIYSGKREKLTSFPRHNGAPRFSPDGKTLALVLSKTGNLQVYTMDLATRRL
CCCEEEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECHHHHHH
TEVTSGRSNNTEPFWHPDGKSLIFTSDRGGKPQIYQVNLSGGETKRLTWQGSQNLGGQIT
HHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEC
PDGKFLVMVNRSDSGFNLAKQDLETGAMQILTKTLLDESPSIAPNGGMVIYSSIYNKANV
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCE
LSMVSIDGRFKARLPATNGRVRAPAWSPFL
EEEEEECCEEEEECCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10692381