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The map label for this gene is orfA [H]

Identifier: 227081935

GI number: 227081935

Start: 1885521

End: 1885865

Strand: Reverse

Name: orfA [H]

Synonym: VCM66_1728

Alternate gene names: 227081935

Gene position: 1885865-1885521 (Counterclockwise)

Preceding gene: 227081938

Following gene: 227081934

Centisome position: 65.2

GC content: 43.19

Gene sequence:

>345_bases
GTGATATCATCACCTCATAAGTTAACAGGTGACATTATGACAAAACGTACAAGACGACTATTTAGCGCAGAATTTAAGTT
AGAAGCAGCGCAGCTAGTCTTAGACCAAAATTACTCAGTGACGGAAGCAGCCCAAGCCATGAATGTGGGCAAGTCCACGA
TGGATAAATGGGTTCGCCAGCTTAGAGAAGAACGCCAAGGGAAAACACCTAAAGCTTCACCTATGACCCCTGAGCAAATA
GAAATTCGGGAATTGAAAAAGAAGCTGGCTCGCCTTGAAGAGCATAATGAAATACTAAAAAAAGCCACGGCTCTCTTGAT
GTCGGACTCACTGAACAATTCTTGA

Upstream 100 bases:

>100_bases
TTCCGTTGCGACATAGGCGAGAAAGTTACTCTCCCATAACTGATAGAGCTCTCGCGCATCACGGTGTAGTGGCATAGTGA
ATTTGGTCACTTAGTTAGAG

Downstream 100 bases:

>100_bases
TAATCAAGAAACTCAAGCAGAGCCACAGCGTAAAAATATTATGCGAAGTCTTCAATGTTCATCGAAGTAGTTATCACTAT
TGGCTTAAACGCCCAGCGTT

Product: ISVch4 transposase, OrfA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 114; Mature: 114

Protein sequence:

>114_residues
MISSPHKLTGDIMTKRTRRLFSAEFKLEAAQLVLDQNYSVTEAAQAMNVGKSTMDKWVRQLREERQGKTPKASPMTPEQI
EIRELKKKLARLEEHNEILKKATALLMSDSLNNS

Sequences:

>Translated_114_residues
MISSPHKLTGDIMTKRTRRLFSAEFKLEAAQLVLDQNYSVTEAAQAMNVGKSTMDKWVRQLREERQGKTPKASPMTPEQI
EIRELKKKLARLEEHNEILKKATALLMSDSLNNS
>Mature_114_residues
MISSPHKLTGDIMTKRTRRLFSAEFKLEAAQLVLDQNYSVTEAAQAMNVGKSTMDKWVRQLREERQGKTPKASPMTPEQI
EIRELKKKLARLEEHNEILKKATALLMSDSLNNS

Specific function: Involved in the transposition of the insertion sequence IS911 [H]

COG id: COG2963

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 8 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002514 [H]

Pfam domain/function: PF01527 Transposase_8 [H]

EC number: NA

Molecular weight: Translated: 13027; Mature: 13027

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISSPHKLTGDIMTKRTRRLFSAEFKLEAAQLVLDQNYSVTEAAQAMNVGKSTMDKWVRQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHH
LREERQGKTPKASPMTPEQIEIRELKKKLARLEEHNEILKKATALLMSDSLNNS
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MISSPHKLTGDIMTKRTRRLFSAEFKLEAAQLVLDQNYSVTEAAQAMNVGKSTMDKWVRQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHH
LREERQGKTPKASPMTPEQIEIRELKKKLARLEEHNEILKKATALLMSDSLNNS
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2163395 [H]