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The map label for this gene is nanM

Identifier: 227081919

GI number: 227081919

Start: 1871028

End: 1872182

Strand: Reverse

Name: nanM

Synonym: VCM66_1712

Alternate gene names: 227081919

Gene position: 1872182-1871028 (Counterclockwise)

Preceding gene: 227081920

Following gene: 227081918

Centisome position: 64.72

GC content: 41.65

Gene sequence:

>1155_bases
ATGAATATGAAAACTCTATTGACTTACGCTACGTTACTTTCTGTGACAGCATTTAGTCATGTGGTCTATGCCGATAATCA
ATGGCCTGATTTACCTACTGGATTTAAAGATGGGGTTGGTGCACAAGTTGGTTCAAAAGTGTATGTAGGACTTGGATCTC
TGGGGAAAAGCTTTTATGTACTTGATTTGAATGCATTATCGAAAGGATGGCAAAAGATTGCTGATTTTACCGGTGCTGAA
CGAAGTGGAGCGACGGCATCTGTAATAGGAAATTATATTTATCTCTTTGGTGGCTCCGGCAAAGCAGAACCGTCTGATCC
ATCGCCTATTTTGTTTGATTCGGTATATCGCTACGACACCAAGAAAGATTCATGGGAGAAAATGAATACGACCTCGCCTG
TAGGACTTCTTGGTGCTTCTTCTTATTCTCCGGACAATAGACAGATCCTCTTTTTTGGTGGTTATAACAAAGCCTATTTT
GATCGCTATCTGCGAGATATATCGACCACTGACAAGCAAGTAAATCCTGAAGTATGGCAACGTATTGTTGATGATTATAT
GGGAATGACGCCTACGGATTATAAATGGAACCGAAACGTGATCAGTTATTTGCCTGAAAAACAAGAGTGGCGTGATTTAG
GCGTGTCTACGTATCTCCCTAATTGTGGTAGCGCAACCGTTATTGAAGGGAACAAAGTAACTCTCATCAGTGGTGAGATA
AAACCGGGTTTACGCACCGCTGAAGTTAAGCAATATGAATTTGGAATGGATCAACCGTGGAAAAGTTTGTTGCCCTTACC
TGCACCCCAAACGAGCAATATCCAAGAGGGTGTTGCAGGTGCATTTTCTGGGAAGACAAATGGAGTTGTAGTTGTTGCTG
GTGGAGCGAATTTCCATGGTGCGAAACAAGCATTTGAAAACGGAAAAATGTTCGCACATGAAGGGCTGCCTAAGGCGTTT
AATTCCGAAATTTATGTAGAAAAAGAGGGCATTTGGTCAACGGTAAATTCGCTTCCTGAGGGCTTGGCATATGGGGCTTC
ATTTACTACATCAGAGGGTGTTTTAATTGTTGGTGGAGAAAAAAGTGGTAAAGAAATGAGTCATAAGGTTTATATGTTGG
CTTGGAATGGCTCTACTGTTGAAGTGATAGACTAA

Upstream 100 bases:

>100_bases
TTAATGGTGAAGAAATTAATCTTCAAAACAAGGTGTTAATGTTCAGTTTCTCATTGAACACCTGACAAAAGTATGGATAG
CAATTAATAAGGACAAATTA

Downstream 100 bases:

>100_bases
TTAAGGAGAGCATTATGGCAATCTCATTTCAATTACTCCCTGAGCTTCCAGTAGGAGTTAAACACGGAGTGGGTGGAATA
ATAGGTCAAAAATTGTATGC

Product: N-acetylneuraminic acid mutarotase

Products: NA

Alternate protein names: N-acetylneuraminate mutarotase; Neu5Ac mutarotase; Sialic acid epimerase

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MNMKTLLTYATLLSVTAFSHVVYADNQWPDLPTGFKDGVGAQVGSKVYVGLGSLGKSFYVLDLNALSKGWQKIADFTGAE
RSGATASVIGNYIYLFGGSGKAEPSDPSPILFDSVYRYDTKKDSWEKMNTTSPVGLLGASSYSPDNRQILFFGGYNKAYF
DRYLRDISTTDKQVNPEVWQRIVDDYMGMTPTDYKWNRNVISYLPEKQEWRDLGVSTYLPNCGSATVIEGNKVTLISGEI
KPGLRTAEVKQYEFGMDQPWKSLLPLPAPQTSNIQEGVAGAFSGKTNGVVVVAGGANFHGAKQAFENGKMFAHEGLPKAF
NSEIYVEKEGIWSTVNSLPEGLAYGASFTTSEGVLIVGGEKSGKEMSHKVYMLAWNGSTVEVID

Sequences:

>Translated_384_residues
MNMKTLLTYATLLSVTAFSHVVYADNQWPDLPTGFKDGVGAQVGSKVYVGLGSLGKSFYVLDLNALSKGWQKIADFTGAE
RSGATASVIGNYIYLFGGSGKAEPSDPSPILFDSVYRYDTKKDSWEKMNTTSPVGLLGASSYSPDNRQILFFGGYNKAYF
DRYLRDISTTDKQVNPEVWQRIVDDYMGMTPTDYKWNRNVISYLPEKQEWRDLGVSTYLPNCGSATVIEGNKVTLISGEI
KPGLRTAEVKQYEFGMDQPWKSLLPLPAPQTSNIQEGVAGAFSGKTNGVVVVAGGANFHGAKQAFENGKMFAHEGLPKAF
NSEIYVEKEGIWSTVNSLPEGLAYGASFTTSEGVLIVGGEKSGKEMSHKVYMLAWNGSTVEVID
>Mature_384_residues
MNMKTLLTYATLLSVTAFSHVVYADNQWPDLPTGFKDGVGAQVGSKVYVGLGSLGKSFYVLDLNALSKGWQKIADFTGAE
RSGATASVIGNYIYLFGGSGKAEPSDPSPILFDSVYRYDTKKDSWEKMNTTSPVGLLGASSYSPDNRQILFFGGYNKAYF
DRYLRDISTTDKQVNPEVWQRIVDDYMGMTPTDYKWNRNVISYLPEKQEWRDLGVSTYLPNCGSATVIEGNKVTLISGEI
KPGLRTAEVKQYEFGMDQPWKSLLPLPAPQTSNIQEGVAGAFSGKTNGVVVVAGGANFHGAKQAFENGKMFAHEGLPKAF
NSEIYVEKEGIWSTVNSLPEGLAYGASFTTSEGVLIVGGEKSGKEMSHKVYMLAWNGSTVEVID

Specific function: Converts alpha-N-acetylneuranimic acid (Neu5Ac) to the beta-anomer, accelerating the equilibrium between the alpha- and beta-anomers. Probably facilitates sialidase-negative bacteria to compete sucessfully for limited amounts of extracellular Neu5Ac, whic

COG id: COG3055

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 7 Kelch repeats

Homologues:

Organism=Escherichia coli, GI87082419, Length=361, Percent_Identity=35.1800554016621, Blast_Score=213, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NANM_VIBC3 (A5F7B3)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F7B3
- SMR:   A5F7B3
- STRING:   A5F7B3
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1372
- eggNOG:   COG3055
- HOGENOM:   HBG365569
- OMA:   FTTAEII
- ProtClustDB:   PRK14131
- BioCyc:   VCHO345073:VC0395_A1372-MONOMER
- HAMAP:   MF_01195
- InterPro:   IPR015915
- InterPro:   IPR006652
- InterPro:   IPR019936
- Gene3D:   G3DSA:2.120.10.80
- TIGRFAMs:   TIGR03547

Pfam domain/function: PF01344 Kelch_1

EC number: NA

Molecular weight: Translated: 41945; Mature: 41945

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: ACT_SITE 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMKTLLTYATLLSVTAFSHVVYADNQWPDLPTGFKDGVGAQVGSKVYVGLGSLGKSFYV
CCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEE
LDLNALSKGWQKIADFTGAERSGATASVIGNYIYLFGGSGKAEPSDPSPILFDSVYRYDT
EEHHHHHHHHHHHHHHCCCCCCCCHHHEECCEEEEECCCCCCCCCCCCCCHHHHHHHCCC
KKDSWEKMNTTSPVGLLGASSYSPDNRQILFFGGYNKAYFDRYLRDISTTDKQVNPEVWQ
CCCCHHHCCCCCCCEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHH
RIVDDYMGMTPTDYKWNRNVISYLPEKQEWRDLGVSTYLPNCGSATVIEGNKVTLISGEI
HHHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHCCCHHCCCCCCCEEEEECCEEEEEECCC
KPGLRTAEVKQYEFGMDQPWKSLLPLPAPQTSNIQEGVAGAFSGKTNGVVVVAGGANFHG
CCCCCCHHHHHHHCCCCCCHHHHCCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCCCCCH
AKQAFENGKMFAHEGLPKAFNSEIYVEKEGIWSTVNSLPEGLAYGASFTTSEGVLIVGGE
HHHHHHCCCEEEECCCCHHHCCCEEEECCCHHHHHHHHHHHHHCCCCEECCCCEEEECCC
KSGKEMSHKVYMLAWNGSTVEVID
CCCCCCCCEEEEEEECCCEEEEEC
>Mature Secondary Structure
MNMKTLLTYATLLSVTAFSHVVYADNQWPDLPTGFKDGVGAQVGSKVYVGLGSLGKSFYV
CCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEE
LDLNALSKGWQKIADFTGAERSGATASVIGNYIYLFGGSGKAEPSDPSPILFDSVYRYDT
EEHHHHHHHHHHHHHHCCCCCCCCHHHEECCEEEEECCCCCCCCCCCCCCHHHHHHHCCC
KKDSWEKMNTTSPVGLLGASSYSPDNRQILFFGGYNKAYFDRYLRDISTTDKQVNPEVWQ
CCCCHHHCCCCCCCEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHH
RIVDDYMGMTPTDYKWNRNVISYLPEKQEWRDLGVSTYLPNCGSATVIEGNKVTLISGEI
HHHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHCCCHHCCCCCCCEEEEECCEEEEEECCC
KPGLRTAEVKQYEFGMDQPWKSLLPLPAPQTSNIQEGVAGAFSGKTNGVVVVAGGANFHG
CCCCCCHHHHHHHCCCCCCHHHHCCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCCCCCH
AKQAFENGKMFAHEGLPKAFNSEIYVEKEGIWSTVNSLPEGLAYGASFTTSEGVLIVGGE
HHHHHHCCCEEEECCCCHHHCCCEEEECCCHHHHHHHHHHHHHCCCCEECCCCEEEECCC
KSGKEMSHKVYMLAWNGSTVEVID
CCCCCCCCEEEEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA