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The map label for this gene is mukF [H]

Identifier: 227081865

GI number: 227081865

Start: 1801488

End: 1802825

Strand: Reverse

Name: mukF [H]

Synonym: VCM66_1656

Alternate gene names: 227081865

Gene position: 1802825-1801488 (Counterclockwise)

Preceding gene: 227081866

Following gene: 227081864

Centisome position: 62.33

GC content: 50.15

Gene sequence:

>1338_bases
ATGAGTGAGTTTACTCAGGATACTGTGCAAAAGCCCATCGACGAACTCGTCACTTGGGTAAAACAGTATGACTTCTCATT
GAATCTGCCTACTGAGCGGCTGGCATTTTTGCTCGCGATTGCTGTGCTCAGTAATGAAAGATTTGATGAAGAGCTCGGAG
AAGGGGAACTTCACGATGCATTCGCGATAGTCACGCGCTTGTTTGCAGAATCGGGAGAAGCCTCAGCCTTTCGGGCTAAC
AATGCGATTAATGATCTGGTCAAACAGCGCTTACTAAGCCGCTTTACCAGCGAAATGACGGAAGGTGCCAGCATCTACCG
TTTGACCCCTTTAGCGATCGGCATTACCGATTACTATGTTCGTCATCGCGAATTTTCCAAACTGAAACTATCCATCCAAC
TCTCTATGGTCGCGGATGAGATGGCGAAAGCCGTGGAATCGGCGCAGCAGGGTGGCAGTGTCGCCCATTGGCGCAAAAAC
GTGTTTGGCGTGCTCAAATATTCGGTGAGTGAAATCTTCGATCGCATCGACCTTAACCAGCGTGTGATGGATGAGCAGCA
GCAATCGGTCAAAGAGCAGATTGCGGATCTGCTGAATAAAGATTGGCGCGATGCGATCAACAACTGTGAAGCCTTGCTGT
CTGAGACTTCCGCCACACTGCGTGAGCTGCAAGATACCTTGCAAGCGGCAGGCGATGAGTTGCAAACGCAAATCCTCGAT
ATTCAGGAGTGCGTGTATGGCGATCTGGAATTGGATTTCATCGAAGAGACGCTGTCTGCGCTGCAAATGAAGCTCGACCG
CATTACGAGCTGGGGTCAGCAGTCGATTGATCTTTGGATTGGTTACGATCGCCACGTCCACAAGTTTATTCGTACCGCGA
TTGATATGGACCAGAACCGCGCGTTCAGCCAACGTCTGCGTCAGTCAATGAACGACTATTTTGAACAACCTTGGTATCTC
ACTTACGCCGATGCAGAACGTCTATCCGATCTGCGTGATGAAACCTTGACCCTGCGTGACGAAGAAGTTACCGGTCATGT
GCCGACAGAAGTGGAATACGAAGAGCTGCAACAAGTGAACGATGAACTTGCGCAGCGGATTGGCGACATGCTGAAAGTGC
ATAAAGAGCAGGGCGCAGCAATTGATTTAGCACTTGTGCTGCGTGATTACCTAGCTAGCCATCCACGAACCCATCATTTC
GATTTAGCCCGAATGGTTGTCGACCAAGCGGTAAGACTGGGTTACTCCGAGTCCGACTACCGCGCTATCCAGCCTGACTG
GACGGCGATCAACGATTTTGGCGCAAAGGTACAAGCGAATGTCATCGACCGATATTAA

Upstream 100 bases:

>100_bases
TGGTGGCACTGGAGCGCAAATTCTGCCGTCAGGAGCCTTACCTCTCATTAGGCCGATACATCCACGTATGGGCTAAGAAA
AAACAATAACAGGACACACA

Downstream 100 bases:

>100_bases
TGAATACATGCCAGAGAATCTGGCCAAAGCGATTGCTAACCCGCTATTTCCAGCGCTAGACAGTTTGCTGCGCGCGGGGC
GTCATGTCTCCAGTGACGAT

Product: condesin subunit F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSEFTQDTVQKPIDELVTWVKQYDFSLNLPTERLAFLLAIAVLSNERFDEELGEGELHDAFAIVTRLFAESGEASAFRAN
NAINDLVKQRLLSRFTSEMTEGASIYRLTPLAIGITDYYVRHREFSKLKLSIQLSMVADEMAKAVESAQQGGSVAHWRKN
VFGVLKYSVSEIFDRIDLNQRVMDEQQQSVKEQIADLLNKDWRDAINNCEALLSETSATLRELQDTLQAAGDELQTQILD
IQECVYGDLELDFIEETLSALQMKLDRITSWGQQSIDLWIGYDRHVHKFIRTAIDMDQNRAFSQRLRQSMNDYFEQPWYL
TYADAERLSDLRDETLTLRDEEVTGHVPTEVEYEELQQVNDELAQRIGDMLKVHKEQGAAIDLALVLRDYLASHPRTHHF
DLARMVVDQAVRLGYSESDYRAIQPDWTAINDFGAKVQANVIDRY

Sequences:

>Translated_445_residues
MSEFTQDTVQKPIDELVTWVKQYDFSLNLPTERLAFLLAIAVLSNERFDEELGEGELHDAFAIVTRLFAESGEASAFRAN
NAINDLVKQRLLSRFTSEMTEGASIYRLTPLAIGITDYYVRHREFSKLKLSIQLSMVADEMAKAVESAQQGGSVAHWRKN
VFGVLKYSVSEIFDRIDLNQRVMDEQQQSVKEQIADLLNKDWRDAINNCEALLSETSATLRELQDTLQAAGDELQTQILD
IQECVYGDLELDFIEETLSALQMKLDRITSWGQQSIDLWIGYDRHVHKFIRTAIDMDQNRAFSQRLRQSMNDYFEQPWYL
TYADAERLSDLRDETLTLRDEEVTGHVPTEVEYEELQQVNDELAQRIGDMLKVHKEQGAAIDLALVLRDYLASHPRTHHF
DLARMVVDQAVRLGYSESDYRAIQPDWTAINDFGAKVQANVIDRY
>Mature_444_residues
SEFTQDTVQKPIDELVTWVKQYDFSLNLPTERLAFLLAIAVLSNERFDEELGEGELHDAFAIVTRLFAESGEASAFRANN
AINDLVKQRLLSRFTSEMTEGASIYRLTPLAIGITDYYVRHREFSKLKLSIQLSMVADEMAKAVESAQQGGSVAHWRKNV
FGVLKYSVSEIFDRIDLNQRVMDEQQQSVKEQIADLLNKDWRDAINNCEALLSETSATLRELQDTLQAAGDELQTQILDI
QECVYGDLELDFIEETLSALQMKLDRITSWGQQSIDLWIGYDRHVHKFIRTAIDMDQNRAFSQRLRQSMNDYFEQPWYLT
YADAERLSDLRDETLTLRDEEVTGHVPTEVEYEELQQVNDELAQRIGDMLKVHKEQGAAIDLALVLRDYLASHPRTHHFD
LARMVVDQAVRLGYSESDYRAIQPDWTAINDFGAKVQANVIDRY

Specific function: Involved in chromosome condensation, segregation and cell cycle progression. May participate in facilitating chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division. Not required for mini-F plasmid

COG id: COG3006

COG function: function code D; Uncharacterized protein involved in chromosome partitioning

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Restricted to the nucleoid region (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mukF family [H]

Homologues:

Organism=Escherichia coli, GI1787152, Length=445, Percent_Identity=60.2247191011236, Blast_Score=563, Evalue=1e-161,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005582 [H]

Pfam domain/function: PF03882 KicB [H]

EC number: NA

Molecular weight: Translated: 51209; Mature: 51078

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFTQDTVQKPIDELVTWVKQYDFSLNLPTERLAFLLAIAVLSNERFDEELGEGELHDA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCHHHHH
FAIVTRLFAESGEASAFRANNAINDLVKQRLLSRFTSEMTEGASIYRLTPLAIGITDYYV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHH
RHREFSKLKLSIQLSMVADEMAKAVESAQQGGSVAHWRKNVFGVLKYSVSEIFDRIDLNQ
HHHHHHHHEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
RVMDEQQQSVKEQIADLLNKDWRDAINNCEALLSETSATLRELQDTLQAAGDELQTQILD
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQECVYGDLELDFIEETLSALQMKLDRITSWGQQSIDLWIGYDRHVHKFIRTAIDMDQNR
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCH
AFSQRLRQSMNDYFEQPWYLTYADAERLSDLRDETLTLRDEEVTGHVPTEVEYEELQQVN
HHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHH
DELAQRIGDMLKVHKEQGAAIDLALVLRDYLASHPRTHHFDLARMVVDQAVRLGYSESDY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCC
RAIQPDWTAINDFGAKVQANVIDRY
CCCCCCCHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure 
SEFTQDTVQKPIDELVTWVKQYDFSLNLPTERLAFLLAIAVLSNERFDEELGEGELHDA
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCHHHHH
FAIVTRLFAESGEASAFRANNAINDLVKQRLLSRFTSEMTEGASIYRLTPLAIGITDYYV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHH
RHREFSKLKLSIQLSMVADEMAKAVESAQQGGSVAHWRKNVFGVLKYSVSEIFDRIDLNQ
HHHHHHHHEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
RVMDEQQQSVKEQIADLLNKDWRDAINNCEALLSETSATLRELQDTLQAAGDELQTQILD
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQECVYGDLELDFIEETLSALQMKLDRITSWGQQSIDLWIGYDRHVHKFIRTAIDMDQNR
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCH
AFSQRLRQSMNDYFEQPWYLTYADAERLSDLRDETLTLRDEEVTGHVPTEVEYEELQQVN
HHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHH
DELAQRIGDMLKVHKEQGAAIDLALVLRDYLASHPRTHHFDLARMVVDQAVRLGYSESDY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCC
RAIQPDWTAINDFGAKVQANVIDRY
CCCCCCCHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA