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The map label for this gene is metE

Identifier: 227081853

GI number: 227081853

Start: 1782209

End: 1784494

Strand: Reverse

Name: metE

Synonym: VCM66_1644

Alternate gene names: 227081853

Gene position: 1784494-1782209 (Counterclockwise)

Preceding gene: 227081856

Following gene: 227081844

Centisome position: 61.69

GC content: 50.35

Gene sequence:

>2286_bases
ATGACAACGACACACATTCTTGGTTATCCCCGCATTGGCGAAAAACGCGAACTAAAATTTGCTCTTGAAAAATACTGGCG
TGGTGAGATTGACCAAGCGGCTTTGAAACAGGTCGGTAGTCAAATTCGCCAAAAAAACTGGGCATTGCAAAAAGAAGCCG
GATTAGACTTTGTGACCGCGGGTGACTTTGCTTGGTACGACCATGTACTCACCACCACGCTGTTACTCGGCCATGTGCCT
AAGCGCCATAGCCACGGTTTTCCTGATTTAGATACTTTGTTCCGTGTCGGTCGCGGTCAGTCACAAAATGCTTGTGGTTG
TGGAACGGGTAGCGCGGCTTCTGATATGACCAAGTGGTTTAACACTAACTATCACTATATCGTGCCGGAGTTCAGCTCTA
ACGACACGTTTAATGTGAGCTGGCCACAACTGTTTGAAGAGGTGAATGAAGCGCTGCAAGCGGGTCACGATGTAAAACCT
GTGCTGCTTGGCCCAATCAGTTACCTCTATTTAGGCAAAGAAGTGGAAGAGGGTTTTGATCGGTTAACGTTGTTGCCGCG
TCTATTGACCGCTTACCAAGCGATTCTGAGCAAGCTGGCGAAGCAGGGTGTGCAATGGGTACAAATCGATGAGCCTATTT
TGGCGTTAGAGCTGGAACCCCGTTGGCAAGAAGCATTTAAACTGGCTTATCAGGTGATCCGCGGTGACGTCAAACTGCTG
CTGACCACTTACTTTGATTCAGTGTTAGATACGCTTGATAAGATAGTCGAACTGCCTGTCGATGGTTTGCATGTCGATAT
TTCGGCCGCTCCAGCGCAGCTCGAAACTATTGTGAACCGCTTGCCCTCCGATTGGGTGCTTTCTGCGGGGGTCATTAATG
GCCGTAATGTTTGGCGTGCCGATTTGAGTGCCATTCTGGCTCGTCTACAACCTGTAAAAACCTTGCTGGGTGAGCGTTTG
TGGGTCGCCAGTTCCTGTTCACTGCTGCACAGCCCAGTCGATCTGGATTTGGAAGGCGATCTCAGCGCAGAAACACGCAG
CTGGTTTGCGTTTGCTAAGCAGAAAGTGACGGAAGTGGCCTTGCTGGGGCGTGCATTGGAAGGGGATGCTGCGGCGATTC
TGGCTTGTGATACCTATAGCCAGCCAATTGTTGCGCGTAAGTCTTCTCACATCGTGAACAAGGCATCAGTACAAACGCGC
ATCAACAATATTACTGCTGCGTTGGCAGAGCGTAGTGCCCCTTACATTGAGCGTGCTCATCACCAAGCGGAAGTGTTGGG
TCTGCCTTTCTTACCGACCACAACCATCGGCTCTTTCCCACAAACTGGTGAGATCCGTACTGAGCGTAGCGCGTATCGCC
AAGGCAAACTGAGCGAGCAAGAGTATGTGCAAGCCCTGAAAGGCCATATTGCTGATGCGGTGAAACGCCAAGAAGCACTA
GGGCTAGATGTGCTGGTACACGGTGAAGCGGAACGTAACGACATGGTGGAATACTTTGCAGAAAACCTCGCCGGTTTCCA
AACCACGCAGTTTGGTTGGGTGCAGAGTTACGGTTCACGCTGCGTAAAACCTGCGATTGTGGTGGCGGATATCGAACGTG
AAAAACCGATCACGGTAGAGTGGTCAACTTACGCGCAATCTCTAACTTCTAAGCAGATGAAAGGAATGTTGACGGGGCCT
GTGACCATTCTATGCTGGACCTTCCCACGTGAAGACATCAGTCGCCAAGAGATTGCTCAGCAATTAGCGTTGGCACTGCG
TGATGAAGTGGCGGATCTGCAAGATGCAGGCATCAATATTATTCAAATTGACGAACCCGCGATTCGTGAAGGTTTACCAC
TGAAAAAACGCGATCATCAAACGTATTTAGATTGGGCAGTGCAAGCGTTTAAGATTTCGGCGGGTAGCGCGCGTCCGGAA
ACGCAAATTCACACCCATATGTGTTACAGCGAATTCAACGAGATCATCGAATCGGTTGCGGCACTGGATGCCGACGTGAT
CACCATTGAAACATCGCGCTCCAACATGGAGCTGCTCAAAGCGTTTGAAGAGTTTAACTACCCGAACGAAATTGGCCCAG
GCGTGTACGATATTCACTCGCCTAACATTCCAGCGCAAGCATGGATTGAAGATCTGCTTCGCAAAGCGGCAGAAAAAATT
CCAGCACAGCGTTTGTGGGTCAACCCAGATTGCGGTCTAAAAACCCGTAACTGGCCAGAAGTGGAAGCGGCACTGACCAA
CATGGTCAACGCAGCGAAAGCACTGCGCGCGGAATGGCAAGCTTGA

Upstream 100 bases:

>100_bases
AGGCGTACAACGCGATAGTTTAGCCATCCAGACATCTTTCGATGTGCTGAGGCAGCTTAGAGCTTCTACGACCTATTAAG
AACAGACAAAGGATTTTCAC

Downstream 100 bases:

>100_bases
TATACGCTTCCTACTTGAGGTTTACCTACAACGCCAAGTAGTTTGAGTAAACCGTTAGAAACCGCAAAGGCCTCTTATGA
GGCCTTTGTTCTTTTCGTTT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 761; Mature: 760

Protein sequence:

>761_residues
MTTTHILGYPRIGEKRELKFALEKYWRGEIDQAALKQVGSQIRQKNWALQKEAGLDFVTAGDFAWYDHVLTTTLLLGHVP
KRHSHGFPDLDTLFRVGRGQSQNACGCGTGSAASDMTKWFNTNYHYIVPEFSSNDTFNVSWPQLFEEVNEALQAGHDVKP
VLLGPISYLYLGKEVEEGFDRLTLLPRLLTAYQAILSKLAKQGVQWVQIDEPILALELEPRWQEAFKLAYQVIRGDVKLL
LTTYFDSVLDTLDKIVELPVDGLHVDISAAPAQLETIVNRLPSDWVLSAGVINGRNVWRADLSAILARLQPVKTLLGERL
WVASSCSLLHSPVDLDLEGDLSAETRSWFAFAKQKVTEVALLGRALEGDAAAILACDTYSQPIVARKSSHIVNKASVQTR
INNITAALAERSAPYIERAHHQAEVLGLPFLPTTTIGSFPQTGEIRTERSAYRQGKLSEQEYVQALKGHIADAVKRQEAL
GLDVLVHGEAERNDMVEYFAENLAGFQTTQFGWVQSYGSRCVKPAIVVADIEREKPITVEWSTYAQSLTSKQMKGMLTGP
VTILCWTFPREDISRQEIAQQLALALRDEVADLQDAGINIIQIDEPAIREGLPLKKRDHQTYLDWAVQAFKISAGSARPE
TQIHTHMCYSEFNEIIESVAALDADVITIETSRSNMELLKAFEEFNYPNEIGPGVYDIHSPNIPAQAWIEDLLRKAAEKI
PAQRLWVNPDCGLKTRNWPEVEAALTNMVNAAKALRAEWQA

Sequences:

>Translated_761_residues
MTTTHILGYPRIGEKRELKFALEKYWRGEIDQAALKQVGSQIRQKNWALQKEAGLDFVTAGDFAWYDHVLTTTLLLGHVP
KRHSHGFPDLDTLFRVGRGQSQNACGCGTGSAASDMTKWFNTNYHYIVPEFSSNDTFNVSWPQLFEEVNEALQAGHDVKP
VLLGPISYLYLGKEVEEGFDRLTLLPRLLTAYQAILSKLAKQGVQWVQIDEPILALELEPRWQEAFKLAYQVIRGDVKLL
LTTYFDSVLDTLDKIVELPVDGLHVDISAAPAQLETIVNRLPSDWVLSAGVINGRNVWRADLSAILARLQPVKTLLGERL
WVASSCSLLHSPVDLDLEGDLSAETRSWFAFAKQKVTEVALLGRALEGDAAAILACDTYSQPIVARKSSHIVNKASVQTR
INNITAALAERSAPYIERAHHQAEVLGLPFLPTTTIGSFPQTGEIRTERSAYRQGKLSEQEYVQALKGHIADAVKRQEAL
GLDVLVHGEAERNDMVEYFAENLAGFQTTQFGWVQSYGSRCVKPAIVVADIEREKPITVEWSTYAQSLTSKQMKGMLTGP
VTILCWTFPREDISRQEIAQQLALALRDEVADLQDAGINIIQIDEPAIREGLPLKKRDHQTYLDWAVQAFKISAGSARPE
TQIHTHMCYSEFNEIIESVAALDADVITIETSRSNMELLKAFEEFNYPNEIGPGVYDIHSPNIPAQAWIEDLLRKAAEKI
PAQRLWVNPDCGLKTRNWPEVEAALTNMVNAAKALRAEWQA
>Mature_760_residues
TTTHILGYPRIGEKRELKFALEKYWRGEIDQAALKQVGSQIRQKNWALQKEAGLDFVTAGDFAWYDHVLTTTLLLGHVPK
RHSHGFPDLDTLFRVGRGQSQNACGCGTGSAASDMTKWFNTNYHYIVPEFSSNDTFNVSWPQLFEEVNEALQAGHDVKPV
LLGPISYLYLGKEVEEGFDRLTLLPRLLTAYQAILSKLAKQGVQWVQIDEPILALELEPRWQEAFKLAYQVIRGDVKLLL
TTYFDSVLDTLDKIVELPVDGLHVDISAAPAQLETIVNRLPSDWVLSAGVINGRNVWRADLSAILARLQPVKTLLGERLW
VASSCSLLHSPVDLDLEGDLSAETRSWFAFAKQKVTEVALLGRALEGDAAAILACDTYSQPIVARKSSHIVNKASVQTRI
NNITAALAERSAPYIERAHHQAEVLGLPFLPTTTIGSFPQTGEIRTERSAYRQGKLSEQEYVQALKGHIADAVKRQEALG
LDVLVHGEAERNDMVEYFAENLAGFQTTQFGWVQSYGSRCVKPAIVVADIEREKPITVEWSTYAQSLTSKQMKGMLTGPV
TILCWTFPREDISRQEIAQQLALALRDEVADLQDAGINIIQIDEPAIREGLPLKKRDHQTYLDWAVQAFKISAGSARPET
QIHTHMCYSEFNEIIESVAALDADVITIETSRSNMELLKAFEEFNYPNEIGPGVYDIHSPNIPAQAWIEDLLRKAAEKIP
AQRLWVNPDCGLKTRNWPEVEAALTNMVNAAKALRAEWQA

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=757, Percent_Identity=61.1624834874505, Blast_Score=934, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=774, Percent_Identity=44.4444444444444, Blast_Score=664, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_VIBCH (Q9KRD8)

Other databases:

- EMBL:   AE003852
- PIR:   E82167
- RefSeq:   NP_231340.1
- ProteinModelPortal:   Q9KRD8
- SMR:   Q9KRD8
- GeneID:   2613709
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1704
- TIGR:   VC_1704
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- BRENDA:   2.1.1.14
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 85048; Mature: 84916

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTHILGYPRIGEKRELKFALEKYWRGEIDQAALKQVGSQIRQKNWALQKEAGLDFVTA
CCCCEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCEEEC
GDFAWYDHVLTTTLLLGHVPKRHSHGFPDLDTLFRVGRGQSQNACGCGTGSAASDMTKWF
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
NTNYHYIVPEFSSNDTFNVSWPQLFEEVNEALQAGHDVKPVLLGPISYLYLGKEVEEGFD
CCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHCHHHHHHHH
RLTLLPRLLTAYQAILSKLAKQGVQWVQIDEPILALELEPRWQEAFKLAYQVIRGDVKLL
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHH
LTTYFDSVLDTLDKIVELPVDGLHVDISAAPAQLETIVNRLPSDWVLSAGVINGRNVWRA
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCEEECCCCCCCHHHHH
DLSAILARLQPVKTLLGERLWVASSCSLLHSPVDLDLEGDLSAETRSWFAFAKQKVTEVA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LLGRALEGDAAAILACDTYSQPIVARKSSHIVNKASVQTRINNITAALAERSAPYIERAH
HHHHHCCCCCEEEEEECCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
HQAEVLGLPFLPTTTIGSFPQTGEIRTERSAYRQGKLSEQEYVQALKGHIADAVKRQEAL
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
GLDVLVHGEAERNDMVEYFAENLAGFQTTQFGWVQSYGSRCVKPAIVVADIEREKPITVE
CCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEE
WSTYAQSLTSKQMKGMLTGPVTILCWTFPREDISRQEIAQQLALALRDEVADLQDAGINI
HHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEE
IQIDEPAIREGLPLKKRDHQTYLDWAVQAFKISAGSARPETQIHTHMCYSEFNEIIESVA
EEECCHHHHCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
ALDADVITIETSRSNMELLKAFEEFNYPNEIGPGVYDIHSPNIPAQAWIEDLLRKAAEKI
HCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHC
PAQRLWVNPDCGLKTRNWPEVEAALTNMVNAAKALRAEWQA
CHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTTHILGYPRIGEKRELKFALEKYWRGEIDQAALKQVGSQIRQKNWALQKEAGLDFVTA
CCCEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCEEEC
GDFAWYDHVLTTTLLLGHVPKRHSHGFPDLDTLFRVGRGQSQNACGCGTGSAASDMTKWF
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
NTNYHYIVPEFSSNDTFNVSWPQLFEEVNEALQAGHDVKPVLLGPISYLYLGKEVEEGFD
CCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHCHHHHHHHH
RLTLLPRLLTAYQAILSKLAKQGVQWVQIDEPILALELEPRWQEAFKLAYQVIRGDVKLL
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHH
LTTYFDSVLDTLDKIVELPVDGLHVDISAAPAQLETIVNRLPSDWVLSAGVINGRNVWRA
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCEEECCCCCCCHHHHH
DLSAILARLQPVKTLLGERLWVASSCSLLHSPVDLDLEGDLSAETRSWFAFAKQKVTEVA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LLGRALEGDAAAILACDTYSQPIVARKSSHIVNKASVQTRINNITAALAERSAPYIERAH
HHHHHCCCCCEEEEEECCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
HQAEVLGLPFLPTTTIGSFPQTGEIRTERSAYRQGKLSEQEYVQALKGHIADAVKRQEAL
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
GLDVLVHGEAERNDMVEYFAENLAGFQTTQFGWVQSYGSRCVKPAIVVADIEREKPITVE
CCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEE
WSTYAQSLTSKQMKGMLTGPVTILCWTFPREDISRQEIAQQLALALRDEVADLQDAGINI
HHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEE
IQIDEPAIREGLPLKKRDHQTYLDWAVQAFKISAGSARPETQIHTHMCYSEFNEIIESVA
EEECCHHHHCCCCCCCCCCHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
ALDADVITIETSRSNMELLKAFEEFNYPNEIGPGVYDIHSPNIPAQAWIEDLLRKAAEKI
HCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHC
PAQRLWVNPDCGLKTRNWPEVEAALTNMVNAAKALRAEWQA
CHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301