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The map label for this gene is focA [H]

Identifier: 227081844

GI number: 227081844

Start: 1776404

End: 1777246

Strand: Reverse

Name: focA [H]

Synonym: VCM66_1635

Alternate gene names: 227081844

Gene position: 1777246-1776404 (Counterclockwise)

Preceding gene: 227081853

Following gene: 227081843

Centisome position: 61.44

GC content: 46.38

Gene sequence:

>843_bases
ATGGAACATAACCAATTCGATTCTCTTCTCCCACCCCAGATGGCAGAACGTGCCGCCATTACGGGTGAAGGTAAAGCTAA
AAAAGCGGCGTACAAATCTTTCCTGTTGGCGATTTCTGCTGGCATCCAAATCGGTATTGCCTTTGTTTTTTATACCGTAG
TCACGACTGGTGCCCACGATATGCCTTACGGGGTGACTAAGCTGCTCGGCGGCTTAGCTTTTAGCTTAGGTTTAATCCTA
GTCGTCATTACTGGCGGTGAGCTGTTTACCAGCTCAGTGTTGATTTTGGTGGCGAAAGCGAGTGGTAAAATTTCGTGGAA
AGAGCTGGTTCGCAACTGGACGGTGGTCTACTTCGGTAACTTATGTGGCTCGATTATCTTAGTGTTTATCATGCTCGCCA
CCCGTCAGTTTATGGAAGATGGTGGTCAGCTTGGCTTAAATGCGATGGCGATTTCCCAGCACAAATTGCATCACACCTTC
TTGCAAGCTTTCGCGCTTGGATTGATGTGTAACATCCTAGTTTGTTTGGCGGTTTGGATGACCTTTAGTGCCCGCTCGTT
AACCGATAAAGTGATGGTCTTGATCCTGCCGGTGGCCATGTTTGTCTCTTCCGGTTTTGAGCACTGTATTGCCAATATGT
TCCAAGTACCGATGGCGATTGGGATTAAGTATTTTGCGCCAGAGAGTTTTTGGGCAATGACAGGAGCGAATATCGCTCAG
TACGCAGATCTCAACTTTGTTAACTTCATCGTCAATAACCTCATTCCAGTGACCTTAGGCAATATTGTTGGTGGCGGTGT
GTTTGTTGGCATGTGGTATTGGCTGATCTATCTAAAAGATTAA

Upstream 100 bases:

>100_bases
AAACATTTTTCGTGAACATGACAAATAGCCCTTGAACTCAAGGGGAAATAGAGCGATTATCCGCCACCTCTTCTGTTGTG
TGTCAAAATGTAACAAGCTC

Downstream 100 bases:

>100_bases
TCCTGACAACCATCAGCGCCTCCCCAATACCCCACAATCCCTGAGTGTTACTCGGGGATTTTTTTACCTGTTTTTCCTTG
CACTCATGGGGTTATCATCG

Product: putative formate transporter

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MEHNQFDSLLPPQMAERAAITGEGKAKKAAYKSFLLAISAGIQIGIAFVFYTVVTTGAHDMPYGVTKLLGGLAFSLGLIL
VVITGGELFTSSVLILVAKASGKISWKELVRNWTVVYFGNLCGSIILVFIMLATRQFMEDGGQLGLNAMAISQHKLHHTF
LQAFALGLMCNILVCLAVWMTFSARSLTDKVMVLILPVAMFVSSGFEHCIANMFQVPMAIGIKYFAPESFWAMTGANIAQ
YADLNFVNFIVNNLIPVTLGNIVGGGVFVGMWYWLIYLKD

Sequences:

>Translated_280_residues
MEHNQFDSLLPPQMAERAAITGEGKAKKAAYKSFLLAISAGIQIGIAFVFYTVVTTGAHDMPYGVTKLLGGLAFSLGLIL
VVITGGELFTSSVLILVAKASGKISWKELVRNWTVVYFGNLCGSIILVFIMLATRQFMEDGGQLGLNAMAISQHKLHHTF
LQAFALGLMCNILVCLAVWMTFSARSLTDKVMVLILPVAMFVSSGFEHCIANMFQVPMAIGIKYFAPESFWAMTGANIAQ
YADLNFVNFIVNNLIPVTLGNIVGGGVFVGMWYWLIYLKD
>Mature_280_residues
MEHNQFDSLLPPQMAERAAITGEGKAKKAAYKSFLLAISAGIQIGIAFVFYTVVTTGAHDMPYGVTKLLGGLAFSLGLIL
VVITGGELFTSSVLILVAKASGKISWKELVRNWTVVYFGNLCGSIILVFIMLATRQFMEDGGQLGLNAMAISQHKLHHTF
LQAFALGLMCNILVCLAVWMTFSARSLTDKVMVLILPVAMFVSSGFEHCIANMFQVPMAIGIKYFAPESFWAMTGANIAQ
YADLNFVNFIVNNLIPVTLGNIVGGGVFVGMWYWLIYLKD

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=277, Percent_Identity=54.5126353790614, Blast_Score=318, Evalue=2e-88,
Organism=Escherichia coli, GI1788837, Length=276, Percent_Identity=41.304347826087, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI87082249, Length=219, Percent_Identity=31.5068493150685, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321779, Length=230, Percent_Identity=27.3913043478261, Blast_Score=86, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 30556; Mature: 30556

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS01005 FORMATE_NITRITE_TP_1 ; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHNQFDSLLPPQMAERAAITGEGKAKKAAYKSFLLAISAGIQIGIAFVFYTVVTTGAHD
CCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
MPYGVTKLLGGLAFSLGLILVVITGGELFTSSVLILVAKASGKISWKELVRNWTVVYFGN
CCHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHEEEECCCCCCHHHHHHCCHHEEHHH
LCGSIILVFIMLATRQFMEDGGQLGLNAMAISQHKLHHTFLQAFALGLMCNILVCLAVWM
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFSARSLTDKVMVLILPVAMFVSSGFEHCIANMFQVPMAIGIKYFAPESFWAMTGANIAQ
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHCCCCHHHHHCCCCHHH
YADLNFVNFIVNNLIPVTLGNIVGGGVFVGMWYWLIYLKD
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEHNQFDSLLPPQMAERAAITGEGKAKKAAYKSFLLAISAGIQIGIAFVFYTVVTTGAHD
CCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
MPYGVTKLLGGLAFSLGLILVVITGGELFTSSVLILVAKASGKISWKELVRNWTVVYFGN
CCHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHEEEECCCCCCHHHHHHCCHHEEHHH
LCGSIILVFIMLATRQFMEDGGQLGLNAMAISQHKLHHTFLQAFALGLMCNILVCLAVWM
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFSARSLTDKVMVLILPVAMFVSSGFEHCIANMFQVPMAIGIKYFAPESFWAMTGANIAQ
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHCCCCHHHHHCCCCHHH
YADLNFVNFIVNNLIPVTLGNIVGGGVFVGMWYWLIYLKD
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]