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The map label for this gene is cls [H]

Identifier: 227081821

GI number: 227081821

Start: 1749457

End: 1750911

Strand: Direct

Name: cls [H]

Synonym: VCM66_1610

Alternate gene names: 227081821

Gene position: 1749457-1750911 (Clockwise)

Preceding gene: 227081818

Following gene: 227081822

Centisome position: 60.48

GC content: 48.93

Gene sequence:

>1455_bases
ATGGACAAGGTTTATCACTTCCTCACTTTGCTCAGTATCGGCATCTATTGGCTTTTGGTCGCCGGTGTCACGATTCGCGT
TGTCTTGAAAAGACGTGCGGTCAGCGTTTCTTTAGCTTGGTTGATGGTGATATACATCATCCCGTTTGTGGGGGTGCTCT
GTTATTTCCTGTTTGGGGAACTCAACCTCGGCCGTAAAAGAGCCGAACGTGCTAAAGAGATGTTCACGCCTTTCGGCCAC
TGGTTCCGCTCTCTCAATGATTGCCAAGCCCATGTACAAGAAGGGATGGGAGCACACATCTCACGAATTGATGAGCTGTG
TAACAACCGGATGGGCATACCCGCACTCAGTGGCAATACCTTATCTTTACTCAACTCTCCGAATGAAATCTTGCACGCCA
TCATTGATGATATTGAACGCGCCCAGTTTCAAATTCGTATGGTGTTCTACATCTGGCATCCTGGTGGTTTAGCCGATGCC
GTTGCTTCGGCCGTGATTCAAGCCTCAAAACGCGGTGTCAACGTCAAACTGTTGCTCGATTCTGCTGGCAGCCCACGTTT
TTTCCGTAGCCCTTGGGAAAAAATGATGCGTGATGCCGGAATTGAGGTAGTACAAGCGTTAGAAGTCAGCCCTTGGCGGA
TCTTCCTACGTCGGCTAGATCTGCGCCAACATCGCAAAATCATTGTGATTGACGATGAAATCGCCTACACCGGCTCCATG
AATATGGTTGACCCTGCCTACTTCAAGCAAAATGCAGGCGTGGGCCAGTGGATCGATATTATGGTTCGAGTGACAGGACC
GACCGTGAATGTCCTCTCGGCCATTCATTGCTGGGATTGGGAATTTGAAACCGGCTCACGCATGTTGCCGAAAAATCCGG
AATGCCGCCTCGAACCGAATCAGCCTCAGCATCCAATCCAAGTTGTCCCTTCTGGCCCTGGTATGCCTGAGAATTTAATT
TCTCAAGTGCTGACGTTAGCGATCAACCAAGCCAATCGCTCAGTCTGCATCACTACACCTTATTTCGTGCCAAGCGCAGA
TTTGCTGGCCACATTAAAAATGACGGCGCAACGTGGCATCAAAGTCGACATTATTATTCCGAAAAAGAACGACTCGCTCA
TGGTGCAATGGGCTTCAAGAGCGTTTTACGGCGAATTGCTCGAAGCTGGCGTACAGATCCATGAGTTTGATGGAGGTTTG
TTGCACACCAAGTCTGTCGTGATTGACCAGCAGTTCTGTTTAGTAGGGACGGTGAATCTGGATATGCGCAGCTTATGGCT
TAACTTTGAGCTTACACTCGCAGTGGATGATCTGGAATTTACTCAACAGATGCATTGGCTGCAGCAGCAGTACATTGATC
AGTCTCATTCCGTCGTTTATGCCGAGTGGGAGCAGCGCCCATGGTACAACCGATTCTTGGAGCGCGTATTTTATCTCTTT
AACCCATTATTGTGA

Upstream 100 bases:

>100_bases
GGATAAGCCTAACTATTGTATTAATTTGGCCAGTTCGCTTCCTTCTCTTATGCTTTCGCTTTACTATCCCCTTTGAATAT
CAGTTTGTTATTGGATAGGC

Downstream 100 bases:

>100_bases
TTTTCTCACTGAGTGGGGTTGCCATCCGGCACGGCACCATGGTTTAAATAACGTTTTGTAAAGTCAGACAAGGACAGCAG
ATGTCAGAAGGTAAAAAGAC

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MDKVYHFLTLLSIGIYWLLVAGVTIRVVLKRRAVSVSLAWLMVIYIIPFVGVLCYFLFGELNLGRKRAERAKEMFTPFGH
WFRSLNDCQAHVQEGMGAHISRIDELCNNRMGIPALSGNTLSLLNSPNEILHAIIDDIERAQFQIRMVFYIWHPGGLADA
VASAVIQASKRGVNVKLLLDSAGSPRFFRSPWEKMMRDAGIEVVQALEVSPWRIFLRRLDLRQHRKIIVIDDEIAYTGSM
NMVDPAYFKQNAGVGQWIDIMVRVTGPTVNVLSAIHCWDWEFETGSRMLPKNPECRLEPNQPQHPIQVVPSGPGMPENLI
SQVLTLAINQANRSVCITTPYFVPSADLLATLKMTAQRGIKVDIIIPKKNDSLMVQWASRAFYGELLEAGVQIHEFDGGL
LHTKSVVIDQQFCLVGTVNLDMRSLWLNFELTLAVDDLEFTQQMHWLQQQYIDQSHSVVYAEWEQRPWYNRFLERVFYLF
NPLL

Sequences:

>Translated_484_residues
MDKVYHFLTLLSIGIYWLLVAGVTIRVVLKRRAVSVSLAWLMVIYIIPFVGVLCYFLFGELNLGRKRAERAKEMFTPFGH
WFRSLNDCQAHVQEGMGAHISRIDELCNNRMGIPALSGNTLSLLNSPNEILHAIIDDIERAQFQIRMVFYIWHPGGLADA
VASAVIQASKRGVNVKLLLDSAGSPRFFRSPWEKMMRDAGIEVVQALEVSPWRIFLRRLDLRQHRKIIVIDDEIAYTGSM
NMVDPAYFKQNAGVGQWIDIMVRVTGPTVNVLSAIHCWDWEFETGSRMLPKNPECRLEPNQPQHPIQVVPSGPGMPENLI
SQVLTLAINQANRSVCITTPYFVPSADLLATLKMTAQRGIKVDIIIPKKNDSLMVQWASRAFYGELLEAGVQIHEFDGGL
LHTKSVVIDQQFCLVGTVNLDMRSLWLNFELTLAVDDLEFTQQMHWLQQQYIDQSHSVVYAEWEQRPWYNRFLERVFYLF
NPLL
>Mature_484_residues
MDKVYHFLTLLSIGIYWLLVAGVTIRVVLKRRAVSVSLAWLMVIYIIPFVGVLCYFLFGELNLGRKRAERAKEMFTPFGH
WFRSLNDCQAHVQEGMGAHISRIDELCNNRMGIPALSGNTLSLLNSPNEILHAIIDDIERAQFQIRMVFYIWHPGGLADA
VASAVIQASKRGVNVKLLLDSAGSPRFFRSPWEKMMRDAGIEVVQALEVSPWRIFLRRLDLRQHRKIIVIDDEIAYTGSM
NMVDPAYFKQNAGVGQWIDIMVRVTGPTVNVLSAIHCWDWEFETGSRMLPKNPECRLEPNQPQHPIQVVPSGPGMPENLI
SQVLTLAINQANRSVCITTPYFVPSADLLATLKMTAQRGIKVDIIIPKKNDSLMVQWASRAFYGELLEAGVQIHEFDGGL
LHTKSVVIDQQFCLVGTVNLDMRSLWLNFELTLAVDDLEFTQQMHWLQQQYIDQSHSVVYAEWEQRPWYNRFLERVFYLF
NPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=486, Percent_Identity=52.4691358024691, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI1787007, Length=365, Percent_Identity=27.9452054794521, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 55359; Mature: 55359

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKVYHFLTLLSIGIYWLLVAGVTIRVVLKRRAVSVSLAWLMVIYIIPFVGVLCYFLFGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LNLGRKRAERAKEMFTPFGHWFRSLNDCQAHVQEGMGAHISRIDELCNNRMGIPALSGNT
CCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCE
LSLLNSPNEILHAIIDDIERAQFQIRMVFYIWHPGGLADAVASAVIQASKRGVNVKLLLD
EEECCCCHHHHHHHHHHHHHCEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEE
SAGSPRFFRSPWEKMMRDAGIEVVQALEVSPWRIFLRRLDLRQHRKIIVIDDEIAYTGSM
CCCCCCHHCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHCCEEEEEECCEEECCCC
NMVDPAYFKQNAGVGQWIDIMVRVTGPTVNVLSAIHCWDWEFETGSRMLPKNPECRLEPN
CCCCHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCC
QPQHPIQVVPSGPGMPENLISQVLTLAINQANRSVCITTPYFVPSADLLATLKMTAQRGI
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCC
KVDIIIPKKNDSLMVQWASRAFYGELLEAGVQIHEFDGGLLHTKSVVIDQQFCLVGTVNL
EEEEEEECCCCCEEEEEHHHHHHHHHHHCCCEEEECCCCEEEEHEEEEECCEEEEEEECC
DMRSLWLNFELTLAVDDLEFTQQMHWLQQQYIDQSHSVVYAEWEQRPWYNRFLERVFYLF
CEEEEEEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHH
NPLL
CCCC
>Mature Secondary Structure
MDKVYHFLTLLSIGIYWLLVAGVTIRVVLKRRAVSVSLAWLMVIYIIPFVGVLCYFLFGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LNLGRKRAERAKEMFTPFGHWFRSLNDCQAHVQEGMGAHISRIDELCNNRMGIPALSGNT
CCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCE
LSLLNSPNEILHAIIDDIERAQFQIRMVFYIWHPGGLADAVASAVIQASKRGVNVKLLLD
EEECCCCHHHHHHHHHHHHHCEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEE
SAGSPRFFRSPWEKMMRDAGIEVVQALEVSPWRIFLRRLDLRQHRKIIVIDDEIAYTGSM
CCCCCCHHCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHCCEEEEEECCEEECCCC
NMVDPAYFKQNAGVGQWIDIMVRVTGPTVNVLSAIHCWDWEFETGSRMLPKNPECRLEPN
CCCCHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCC
QPQHPIQVVPSGPGMPENLISQVLTLAINQANRSVCITTPYFVPSADLLATLKMTAQRGI
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCC
KVDIIIPKKNDSLMVQWASRAFYGELLEAGVQIHEFDGGLLHTKSVVIDQQFCLVGTVNL
EEEEEEECCCCCEEEEEHHHHHHHHHHHCCCEEEECCCCEEEEHEEEEECCEEEEEEECC
DMRSLWLNFELTLAVDDLEFTQQMHWLQQQYIDQSHSVVYAEWEQRPWYNRFLERVFYLF
CEEEEEEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHH
NPLL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA