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The map label for this gene is ywbI [H]

Identifier: 227081770

GI number: 227081770

Start: 1677807

End: 1678697

Strand: Reverse

Name: ywbI [H]

Synonym: VCM66_1557

Alternate gene names: 227081770

Gene position: 1678697-1677807 (Counterclockwise)

Preceding gene: 227081773

Following gene: 227081768

Centisome position: 58.04

GC content: 44.56

Gene sequence:

>891_bases
TTGCGATATACAGGATATATGATGGAAGTTCGACAACTGCGTCACTTTGTGACCGTGGCAGAATGGGAAAGTTTTACCTT
AGCCGCGCAGCAACTGCATATTGCGCAGCCTGCACTGAGTATTTCGATTAAGAAATTTGAGCAGCAGCTCGGTGTTACTC
TGTTTAAAAGAGATGAAAGAAAAGTTGCGCTGACCCATGAAGGTGAAGTGTTGCTTGAACATGCCAAGCGCATTTTGCAA
CAAGTCAATGATGCCCAATTGGCGGTTGATGAGCTCAAAGGTGTTGTGAAAGGCGAAGTACGCCTTGGTGCGCCAAGCAT
GATGGGCTCTTACTTTTTCCCGCAAATCATTATGGCGTTTAAAAGCCACTTTCCGAATCTGAAAATGACGGTGATTGAAG
CGGGTACTCAATCGATCCGCAGAATGCTGCTCTCAGGCGAGTTGGATATTGGGGTGATTTTGAATCACGATCTGCCGGAC
GATTTGGCTGCAGATCCGCTGCTCTCTTCGCAAATGGTTGCTGTAGTCGGCAAAAATCATGAACTAGCTGAACGTCGTCA
TCTTACTTTTGCCGAGTTTTTTGAGCATGAACTGGTAATGTTTAAACCCGGCTATTTTCACCGAGATTTTATCGATAAAG
TGTGCCGAGAACATAAATTTACGGCAGATTTTTCTTTTGAAACCAATTTGTTACCTATGATTTTAAGCATAGTGAAACAT
GAATATGCGATTACCGCGCTGTTGGAGTTGGTGACCAGCAATGAACCGGAAGTGAAGGCGATTCCGTTTGAACCGCCGGT
GTGGCTTGATCTTGCATTGGCTTGGCGTAAAGAAGGTTATCTTTCCAATGCCGATCGTACTTTCATCGAGTTTGTGAAAA
AGTACGTTTAG

Upstream 100 bases:

>100_bases
AATGCAGTTCAATCATACAGCGGATCCAAGATGCAAAAAGGCGGTGATGTGATCACGATAGCAGTGGCATCTCGATTAAT
GAAATATATTAAAAATATGG

Downstream 100 bases:

>100_bases
CGCCAAGAAGAGCATTCATCTTTGCTGCTATTAATCTTTACTGCTGTAAATACGTTCAAAATTGGTGTGGTTCCAGCCGA
TCATCAAACGATCACCAATT

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MRYTGYMMEVRQLRHFVTVAEWESFTLAAQQLHIAQPALSISIKKFEQQLGVTLFKRDERKVALTHEGEVLLEHAKRILQ
QVNDAQLAVDELKGVVKGEVRLGAPSMMGSYFFPQIIMAFKSHFPNLKMTVIEAGTQSIRRMLLSGELDIGVILNHDLPD
DLAADPLLSSQMVAVVGKNHELAERRHLTFAEFFEHELVMFKPGYFHRDFIDKVCREHKFTADFSFETNLLPMILSIVKH
EYAITALLELVTSNEPEVKAIPFEPPVWLDLALAWRKEGYLSNADRTFIEFVKKYV

Sequences:

>Translated_296_residues
MRYTGYMMEVRQLRHFVTVAEWESFTLAAQQLHIAQPALSISIKKFEQQLGVTLFKRDERKVALTHEGEVLLEHAKRILQ
QVNDAQLAVDELKGVVKGEVRLGAPSMMGSYFFPQIIMAFKSHFPNLKMTVIEAGTQSIRRMLLSGELDIGVILNHDLPD
DLAADPLLSSQMVAVVGKNHELAERRHLTFAEFFEHELVMFKPGYFHRDFIDKVCREHKFTADFSFETNLLPMILSIVKH
EYAITALLELVTSNEPEVKAIPFEPPVWLDLALAWRKEGYLSNADRTFIEFVKKYV
>Mature_296_residues
MRYTGYMMEVRQLRHFVTVAEWESFTLAAQQLHIAQPALSISIKKFEQQLGVTLFKRDERKVALTHEGEVLLEHAKRILQ
QVNDAQLAVDELKGVVKGEVRLGAPSMMGSYFFPQIIMAFKSHFPNLKMTVIEAGTQSIRRMLLSGELDIGVILNHDLPD
DLAADPLLSSQMVAVVGKNHELAERRHLTFAEFFEHELVMFKPGYFHRDFIDKVCREHKFTADFSFETNLLPMILSIVKH
EYAITALLELVTSNEPEVKAIPFEPPVWLDLALAWRKEGYLSNADRTFIEFVKKYV

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=283, Percent_Identity=29.6819787985866, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI145693105, Length=283, Percent_Identity=28.9752650176678, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790399, Length=260, Percent_Identity=28.4615384615385, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787879, Length=290, Percent_Identity=30, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI2367136, Length=286, Percent_Identity=27.2727272727273, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788887, Length=200, Percent_Identity=32, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788297, Length=162, Percent_Identity=33.9506172839506, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788481, Length=289, Percent_Identity=23.8754325259516, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788748, Length=289, Percent_Identity=24.9134948096886, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1787601, Length=156, Percent_Identity=31.4102564102564, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1788296, Length=204, Percent_Identity=27.4509803921569, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87081904, Length=213, Percent_Identity=28.6384976525822, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082132, Length=294, Percent_Identity=25.5102040816327, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786401, Length=159, Percent_Identity=30.188679245283, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789204, Length=243, Percent_Identity=23.8683127572016, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1787530, Length=235, Percent_Identity=22.1276595744681, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789506, Length=277, Percent_Identity=26.3537906137184, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787589, Length=194, Percent_Identity=28.3505154639175, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=196, Percent_Identity=26.530612244898, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789639, Length=128, Percent_Identity=25.78125, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790208, Length=145, Percent_Identity=26.8965517241379, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787806, Length=160, Percent_Identity=26.875, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788706, Length=183, Percent_Identity=27.8688524590164, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1786448, Length=176, Percent_Identity=28.9772727272727, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33991; Mature: 33991

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYTGYMMEVRQLRHFVTVAEWESFTLAAQQLHIAQPALSISIKKFEQQLGVTLFKRDER
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEEECCCC
KVALTHEGEVLLEHAKRILQQVNDAQLAVDELKGVVKGEVRLGAPSMMGSYFFPQIIMAF
EEEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHH
KSHFPNLKMTVIEAGTQSIRRMLLSGELDIGVILNHDLPDDLAADPLLSSQMVAVVGKNH
HHHCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHCCCCCCCCHHEEECCCC
ELAERRHLTFAEFFEHELVMFKPGYFHRDFIDKVCREHKFTADFSFETNLLPMILSIVKH
HHHHHHCCHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
EYAITALLELVTSNEPEVKAIPFEPPVWLDLALAWRKEGYLSNADRTFIEFVKKYV
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MRYTGYMMEVRQLRHFVTVAEWESFTLAAQQLHIAQPALSISIKKFEQQLGVTLFKRDER
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEEECCCC
KVALTHEGEVLLEHAKRILQQVNDAQLAVDELKGVVKGEVRLGAPSMMGSYFFPQIIMAF
EEEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHH
KSHFPNLKMTVIEAGTQSIRRMLLSGELDIGVILNHDLPDDLAADPLLSSQMVAVVGKNH
HHHCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHCCCCCCCCHHEEECCCC
ELAERRHLTFAEFFEHELVMFKPGYFHRDFIDKVCREHKFTADFSFETNLLPMILSIVKH
HHHHHHCCHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
EYAITALLELVTSNEPEVKAIPFEPPVWLDLALAWRKEGYLSNADRTFIEFVKKYV
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]