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The map label for this gene is nagK

Identifier: 227081688

GI number: 227081688

Start: 1592476

End: 1593384

Strand: Reverse

Name: nagK

Synonym: VCM66_1474

Alternate gene names: 227081688

Gene position: 1593384-1592476 (Counterclockwise)

Preceding gene: 227081689

Following gene: 227081686

Centisome position: 55.09

GC content: 49.72

Gene sequence:

>909_bases
ATGTATTACGGCTTTGATGTGGGCGGAACCAAAATTGAATTTGGTGCGTTCAATGAACAGTTAGAACGCGTTGCCACGGA
ACGCGTCGCGACACCAACGGATGACTATGCCAAATTGGTGGAGACTATTGCCGGGTTAGTCCATAAATACGATGCGCAAT
TTGGCGTCGAAGGTACGGTTGGTCTGGGTATTCCGGGCATGGAAGATGCCGATAATGGCTGCGTATTGACCGTTAACGTG
CCTGCAGCCAAAGGCAAACCGCTGCGTGCCGATTTGGAAACCAAACTCGGCCGCGCAGTGAAAGTGGAAAATGATGCGAA
CTGTTTTGCGCTCTCTGAAGCTTGGGATGATGAGCTAAAAGAAGCGGCTTCCGTGATGGGCTTGATTCTAGGTACCGGTT
TTGGTGGCGGACTGGTCTACGAAGGTAAAGTGTTCTCAGGTCGTAACCACGTAGCGGGTGAAATTGGCCACATGCGCCTA
CCGATTGATGCTTGGTTCCATCTTGGTGAAAAAGCCCCATTACTGGGCTGTGGCTGCGGCAATAAAGGTTGTATGGACAA
CTATCTCTCTGGCCGTGGCTTTGAGCTGTTATACGAGCACTATTACGGCGAAAAGAAAAAAGCGATTGATATCATCACCG
CACAGAAAGAGGGTGAATCCAAAGCCGTTGAGCACGTTGAGCGCTTTATGGAATTGCTGGCGATCTGTTTCGCCAATATT
TTTACTGCCAACGATCCACACGTGGTGGTGTTAGGCGGCGGATTATCAAACTACGATCTGATTTATGAAGAAATGCCAAA
GCGTGTGCCTAAGCATCTGCTCTCTGTTGCGAAATGCCCGAAAATCGTTAAAGCCAAACATGGCGACTCCGGCGGCGTGC
GCGGAGCGGCTTTCCTCAACATTAAATAA

Upstream 100 bases:

>100_bases
TTTGTCACGGACTGTTGAGGGTAGCTTGTGGATAATACGCCTCACTTTTGTGTTTTTATTTTTTCCCAAGGCTGGGATAG
GAAATTGAGGAGAGAAGGCC

Downstream 100 bases:

>100_bases
GTTGAAGATCAACAGAATAAATTAAGGGCTTGCACTGCAAGCCCTTTTCCCCTTCCTACTTGAAGTTGCAGCGGTGTTGG
CTGCGTTCATTCACCCCAAT

Product: N-acetyl-D-glucosamine kinase

Products: NA

Alternate protein names: GlcNAc kinase

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MYYGFDVGGTKIEFGAFNEQLERVATERVATPTDDYAKLVETIAGLVHKYDAQFGVEGTVGLGIPGMEDADNGCVLTVNV
PAAKGKPLRADLETKLGRAVKVENDANCFALSEAWDDELKEAASVMGLILGTGFGGGLVYEGKVFSGRNHVAGEIGHMRL
PIDAWFHLGEKAPLLGCGCGNKGCMDNYLSGRGFELLYEHYYGEKKKAIDIITAQKEGESKAVEHVERFMELLAICFANI
FTANDPHVVVLGGGLSNYDLIYEEMPKRVPKHLLSVAKCPKIVKAKHGDSGGVRGAAFLNIK

Sequences:

>Translated_302_residues
MYYGFDVGGTKIEFGAFNEQLERVATERVATPTDDYAKLVETIAGLVHKYDAQFGVEGTVGLGIPGMEDADNGCVLTVNV
PAAKGKPLRADLETKLGRAVKVENDANCFALSEAWDDELKEAASVMGLILGTGFGGGLVYEGKVFSGRNHVAGEIGHMRL
PIDAWFHLGEKAPLLGCGCGNKGCMDNYLSGRGFELLYEHYYGEKKKAIDIITAQKEGESKAVEHVERFMELLAICFANI
FTANDPHVVVLGGGLSNYDLIYEEMPKRVPKHLLSVAKCPKIVKAKHGDSGGVRGAAFLNIK
>Mature_302_residues
MYYGFDVGGTKIEFGAFNEQLERVATERVATPTDDYAKLVETIAGLVHKYDAQFGVEGTVGLGIPGMEDADNGCVLTVNV
PAAKGKPLRADLETKLGRAVKVENDANCFALSEAWDDELKEAASVMGLILGTGFGGGLVYEGKVFSGRNHVAGEIGHMRL
PIDAWFHLGEKAPLLGCGCGNKGCMDNYLSGRGFELLYEHYYGEKKKAIDIITAQKEGESKAVEHVERFMELLAICFANI
FTANDPHVVVLGGGLSNYDLIYEEMPKRVPKHLLSVAKCPKIVKAKHGDSGGVRGAAFLNIK

Specific function: Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family. NagK subfamily

Homologues:

Organism=Escherichia coli, GI1787363, Length=301, Percent_Identity=53.156146179402, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI87081733, Length=304, Percent_Identity=38.8157894736842, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1790522, Length=307, Percent_Identity=27.0358306188925, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786891, Length=232, Percent_Identity=24.5689655172414, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NAGK_VIBCH (Q9KRV2)

Other databases:

- EMBL:   AE003852
- PIR:   B82189
- RefSeq:   NP_231172.1
- HSSP:   Q8ZPZ9
- ProteinModelPortal:   Q9KRV2
- SMR:   Q9KRV2
- GeneID:   2613911
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1532
- TIGR:   VC_1532
- HOGENOM:   HBG730260
- OMA:   GMEDADD
- ProtClustDB:   PRK13310
- BRENDA:   2.7.1.59
- HAMAP:   MF_01271
- InterPro:   IPR000600

Pfam domain/function: PF00480 ROK

EC number: =2.7.1.59

Molecular weight: Translated: 32604; Mature: 32604

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS01125 ROK; PS00963 RIBOSOMAL_S2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYGFDVGGTKIEFGAFNEQLERVATERVATPTDDYAKLVETIAGLVHKYDAQFGVEGTV
CEEEECCCCCEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEE
GLGIPGMEDADNGCVLTVNVPAAKGKPLRADLETKLGRAVKVENDANCFALSEAWDDELK
ECCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCEEEECCCCCEEEEHHHHHHHHH
EAASVMGLILGTGFGGGLVYEGKVFSGRNHVAGEIGHMRLPIDAWFHLGEKAPLLGCGCG
HHHHHHHHHHHCCCCCCEEEECEEECCCCCCCCCCCCEECCHHHHHHCCCCCCEEEECCC
NKGCMDNYLSGRGFELLYEHYYGEKKKAIDIITAQKEGESKAVEHVERFMELLAICFANI
CCCCHHHHCCCCCHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
FTANDPHVVVLGGGLSNYDLIYEEMPKRVPKHLLSVAKCPKIVKAKHGDSGGVRGAAFLN
HCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEEE
IK
CC
>Mature Secondary Structure
MYYGFDVGGTKIEFGAFNEQLERVATERVATPTDDYAKLVETIAGLVHKYDAQFGVEGTV
CEEEECCCCCEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEE
GLGIPGMEDADNGCVLTVNVPAAKGKPLRADLETKLGRAVKVENDANCFALSEAWDDELK
ECCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCEEEECCCCCEEEEHHHHHHHHH
EAASVMGLILGTGFGGGLVYEGKVFSGRNHVAGEIGHMRLPIDAWFHLGEKAPLLGCGCG
HHHHHHHHHHHCCCCCCEEEECEEECCCCCCCCCCCCEECCHHHHHHCCCCCCEEEECCC
NKGCMDNYLSGRGFELLYEHYYGEKKKAIDIITAQKEGESKAVEHVERFMELLAICFANI
CCCCHHHHCCCCCHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
FTANDPHVVVLGGGLSNYDLIYEEMPKRVPKHLLSVAKCPKIVKAKHGDSGGVRGAAFLN
HCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEEE
IK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301