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The map label for this gene is pyrD

Identifier: 227081650

GI number: 227081650

Start: 1544299

End: 1545309

Strand: Reverse

Name: pyrD

Synonym: VCM66_1434

Alternate gene names: 227081650

Gene position: 1545309-1544299 (Counterclockwise)

Preceding gene: 227081651

Following gene: 227081649

Centisome position: 53.42

GC content: 45.4

Gene sequence:

>1011_bases
ATGCTTTACCGCTTAGCCAGAGCTGGCTTTTTCCAATTGGATGCCGAAAAGGCACACGATCTGGCCATCTCTAATTTCAA
ACGTTTCACTGGCACTCCTTTCGATCTCTTCTATCGTCAACAACTTCCTCATCGTCCAGTTCAATGCATGGGCTTAACCT
TTAAAAATCCAGTCGGTTTAGCAGCAGGGCTCGACAAAAACGGCGAGTGCATCGAAGCGTTTGGCGCGATGGGCTTCGGA
TTTGTTGAAGTAGGCACGGTCACACCAAGACCACAAGCAGGTAACGACAAACCACGCCTGTTTCGTTTAGTGCATGCTGA
AGGCATCATCAATCGAATGGGCTTTAACAATCTGGGTGTTGATCACTTGGTTGAGAATGTTAAGCGAGCCAAATACGATG
GGATCATCGGGATCAACATCGGTAAAAACAAAGATACTCCGATTGAGAAAGGGGCAGAGGACTATTTGATCTGTATGGAT
AAAGTTTATCCTTACGCAGGTTACATCGCCGTAAATATCTCTTCTCCGAACACACCAGGACTTCGTTCTCTACAATACGG
TGAAGCGCTGGATGAACTGCTTGCTGCATTGAAAACTCGCCAAGCTGAATTAGCAGCGAAACATGATAAATATGTCCCGC
TTGCACTTAAGATTGCACCAGATTTAAGTGACGATGAAATTCAGCAAATCTGCCAATCACTTTTGAAAAACAAAATCGAT
AGTGTCATCGCGACAAACACCACCTTAGATCGTTCATTGGTTGAAGGGATGAAGTTTGCCAACGAAGCTGGCGGCCTCAG
TGGACGACCTTTGCAAAACCGCAGTACAGAAGTTATTAAGTGTCTGTATAAAGAACTCGGTGAAGAAATTCCGATCATCG
GGGTCGGTGGTATCGATTCCTACATCTCCGCCAAAGAAAAGCTCTTAGCAGGAGCAAAATTAGTTCAGGTCTATAGCGGC
TTTATTTATCAAGGACCAGGGCTGGTCGCCGATATCGTCAAGAACCTGTAA

Upstream 100 bases:

>100_bases
AACTTAAATTGTCTCACTGTAGAATAAGCATTGAATTGCCTGACGAATGAGTCAATAATAACGCCCCGTTTATTCGGGGC
TTTTTTCTTTCGGAGGCATT

Downstream 100 bases:

>100_bases
GTTCCCCGATTTATCAGCGGAATTACTGAGGAAATGTCTAACGCATTTCCTCTTTTTTTTTCTCTGCACCTTCATAGAAT
TACTGATATTCATAGGCAAG

Product: dihydroorotate dehydrogenase 2

Products: NA

Alternate protein names: DHOdehase; DHOD; DHODase; Dihydroorotate oxidase

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MLYRLARAGFFQLDAEKAHDLAISNFKRFTGTPFDLFYRQQLPHRPVQCMGLTFKNPVGLAAGLDKNGECIEAFGAMGFG
FVEVGTVTPRPQAGNDKPRLFRLVHAEGIINRMGFNNLGVDHLVENVKRAKYDGIIGINIGKNKDTPIEKGAEDYLICMD
KVYPYAGYIAVNISSPNTPGLRSLQYGEALDELLAALKTRQAELAAKHDKYVPLALKIAPDLSDDEIQQICQSLLKNKID
SVIATNTTLDRSLVEGMKFANEAGGLSGRPLQNRSTEVIKCLYKELGEEIPIIGVGGIDSYISAKEKLLAGAKLVQVYSG
FIYQGPGLVADIVKNL

Sequences:

>Translated_336_residues
MLYRLARAGFFQLDAEKAHDLAISNFKRFTGTPFDLFYRQQLPHRPVQCMGLTFKNPVGLAAGLDKNGECIEAFGAMGFG
FVEVGTVTPRPQAGNDKPRLFRLVHAEGIINRMGFNNLGVDHLVENVKRAKYDGIIGINIGKNKDTPIEKGAEDYLICMD
KVYPYAGYIAVNISSPNTPGLRSLQYGEALDELLAALKTRQAELAAKHDKYVPLALKIAPDLSDDEIQQICQSLLKNKID
SVIATNTTLDRSLVEGMKFANEAGGLSGRPLQNRSTEVIKCLYKELGEEIPIIGVGGIDSYISAKEKLLAGAKLVQVYSG
FIYQGPGLVADIVKNL
>Mature_336_residues
MLYRLARAGFFQLDAEKAHDLAISNFKRFTGTPFDLFYRQQLPHRPVQCMGLTFKNPVGLAAGLDKNGECIEAFGAMGFG
FVEVGTVTPRPQAGNDKPRLFRLVHAEGIINRMGFNNLGVDHLVENVKRAKYDGIIGINIGKNKDTPIEKGAEDYLICMD
KVYPYAGYIAVNISSPNTPGLRSLQYGEALDELLAALKTRQAELAAKHDKYVPLALKIAPDLSDDEIQQICQSLLKNKID
SVIATNTTLDRSLVEGMKFANEAGGLSGRPLQNRSTEVIKCLYKELGEEIPIIGVGGIDSYISAKEKLLAGAKLVQVYSG
FIYQGPGLVADIVKNL

Specific function: Pyrimidine biosynthesis; fourth step. [C]

COG id: COG0167

COG function: function code F; Dihydroorotate dehydrogenase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily

Homologues:

Organism=Homo sapiens, GI45006951, Length=336, Percent_Identity=41.9642857142857, Blast_Score=230, Evalue=2e-60,
Organism=Escherichia coli, GI1787177, Length=336, Percent_Identity=67.8571428571429, Blast_Score=492, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17509475, Length=333, Percent_Identity=41.1411411411411, Blast_Score=234, Evalue=6e-62,
Organism=Drosophila melanogaster, GI281361352, Length=328, Percent_Identity=42.6829268292683, Blast_Score=250, Evalue=8e-67,
Organism=Drosophila melanogaster, GI17137316, Length=328, Percent_Identity=42.6829268292683, Blast_Score=250, Evalue=8e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRD_VIBC3 (A5F839)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F839
- SMR:   A5F839
- STRING:   A5F839
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1098
- eggNOG:   COG0167
- HOGENOM:   HBG351027
- OMA:   SYVTVNI
- ProtClustDB:   PRK05286
- BioCyc:   VCHO345073:VC0395_A1098-MONOMER
- HAMAP:   MF_00225
- InterPro:   IPR013785
- InterPro:   IPR012135
- InterPro:   IPR005719
- InterPro:   IPR001295
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000164
- TIGRFAMs:   TIGR01036

Pfam domain/function: PF01180 DHO_dh

EC number: =1.3.5.2

Molecular weight: Translated: 36766; Mature: 36766

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS00911 DHODEHASE_1; PS00912 DHODEHASE_2

Important sites: ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYRLARAGFFQLDAEKAHDLAISNFKRFTGTPFDLFYRQQLPHRPVQCMGLTFKNPVGL
CCEEHHHCCCEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHEECCEECCCCCE
AAGLDKNGECIEAFGAMGFGFVEVGTVTPRPQAGNDKPRLFRLVHAEGIINRMGFNNLGV
EECCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCH
DHLVENVKRAKYDGIIGINIGKNKDTPIEKGAEDYLICMDKVYPYAGYIAVNISSPNTPG
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHEEEHHHCCCCCCEEEEEECCCCCCC
LRSLQYGEALDELLAALKTRQAELAAKHDKYVPLALKIAPDLSDDEIQQICQSLLKNKID
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
SVIATNTTLDRSLVEGMKFANEAGGLSGRPLQNRSTEVIKCLYKELGEEIPIIGVGGIDS
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHH
YISAKEKLLAGAKLVQVYSGFIYQGPGLVADIVKNL
HHHHHHHHHHHHHHHHHHHCHHCCCCCHHHHHHHCC
>Mature Secondary Structure
MLYRLARAGFFQLDAEKAHDLAISNFKRFTGTPFDLFYRQQLPHRPVQCMGLTFKNPVGL
CCEEHHHCCCEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHEECCEECCCCCE
AAGLDKNGECIEAFGAMGFGFVEVGTVTPRPQAGNDKPRLFRLVHAEGIINRMGFNNLGV
EECCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCH
DHLVENVKRAKYDGIIGINIGKNKDTPIEKGAEDYLICMDKVYPYAGYIAVNISSPNTPG
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHEEEHHHCCCCCCEEEEEECCCCCCC
LRSLQYGEALDELLAALKTRQAELAAKHDKYVPLALKIAPDLSDDEIQQICQSLLKNKID
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
SVIATNTTLDRSLVEGMKFANEAGGLSGRPLQNRSTEVIKCLYKELGEEIPIIGVGGIDS
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHH
YISAKEKLLAGAKLVQVYSGFIYQGPGLVADIVKNL
HHHHHHHHHHHHHHHHHHHCHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA