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The map label for this gene is luxQ [H]

Identifier: 227081617

GI number: 227081617

Start: 1498895

End: 1500613

Strand: Direct

Name: luxQ [H]

Synonym: VCM66_1401

Alternate gene names: 227081617

Gene position: 1498895-1500613 (Clockwise)

Preceding gene: 227081616

Following gene: 227081621

Centisome position: 51.82

GC content: 38.92

Gene sequence:

>1719_bases
ATGAAAATGGATTCTGAATACACAGAGAAAGTAAATGAATTGACTCTTGAGTTACAAAGAAGCATTGAACGAGGAAAACA
GCTTGCAGAAGAGAATAGGGTAATTCTCGCTGCTATCTCTGCATTCAGTGTTGCCACCAACAAAAATCATATCTTTGAAG
AATTAAAAACATTATTGAATCGATATATTCAGTTTGATGATTTTATTGTTATTTCCAAAGATAAAAACGGTCACCAGTTT
CGTACGGCGATTGCCTCAAATGCCTCATTTAATGATGTTGTTTGGCCAAACGGAGTTAAATTTCAGCGTGTGCTGAATGG
TGAGTGCATTCTGTTATTTGAACCTTGTAAGTTAGATGAGTTTGCCTGCTTAATGGGGTCGATTGATAAGCCGGTTTATT
CCGCTTTGATTACTGGGATTCAAGCTCAAATCAGCGATTCTGTCCTCATCTTATTAGGGCAGAATAAAGGTCAGTTTTCT
CTTGAAGCAAAAGAGACGTTGTCACGTTTTAGGCCGCTGTTAGAAAGAGCTATCTCAGATATTGAACATAAAGAAGAGTT
ACAAAAACTCGTAGAAATAAGAACGCGTGAACTGCGTTTGGCGCAAAAACTGGCAGAGCAAGCTAACCAATCTAAGTCCA
ACTTCCTCGCGATGATGAGCCATGAGCTCAGAACTCCGTTGAATGCGGTGCTTGGATTGATTGATATTTTACGTGGAGAG
TCAACGAGTTACCAAGTTGAGCTGCTTGAGCAGATGGAAAACTCTGCCGAACTTCTTCTCATTATTATCAATGATATTTT
AGATTTAAGCCGAATCGAGTCTGGGCACTTTTCATTACACTGTCACTGGTTGGATCTAGAAAAGAAACTAGATCAATCTT
TGGTTTATCACAGACAGATTGCGCAAGATAAAGGTGTGCATTTTTATGTTCAATCGAATAAGAATCCAACATTAGAGTAC
TACACCGATTCGGCAAGGCTCACACAGATACTTTTTAATATTGTTGGTAACGCCATCAAGTTCACTAAACAAGGTCAAGT
TGATGTAGTATTAGACTACACCAAGGATGGCGTCGTATTTCGAGTCAAAGATACAGGAATTGGGATTGAAAAGAATCGGG
TAGAACAATTATTTACCCCCTTTATTCAAGCGGATAATTCAATTACAAGAAATTATGGTGGTACAGGGTTAGGCCTTGCG
ATTACCAAACATTTGGTTGAATTGATGGGTGGCACAATCCAAGTTGAAAGTGAGTTCGGTATAGGCACAACCTTTACTTT
ATATATTCCACTTCAATCTCGCTTGAAAAATATTAAACCGAGTACGTCCTCATCCCAACAAATCGTAAATGTAAAAAATC
ATCACATTCTTGTTGTGGAAGACACAAAGACCAATCAAATGGTCATTCAATTACTACTGAATAAGTTGGGCTATGATGTC
ACGATTGCAGAAAATGGGCTGCAAGCGGTGGAGCTTTTAGAAAAAAATCATGTATTTGATCTGGTGCTAATGGATATTTC
CATGCCGATTATGGATGGTATTGCTGCAACCAAAATACTCCGAGATAAACATATTGAAATCCCCATTATCGCACTCACTG
CTCACACCGCCGGGAGTGACAAACAAAACTGTATTGATGCGGGAATGAATGACATTGTACTAAAACCGATCCGCAGCAAA
GACATCATGGAGGTTGTGAAACGTTTCCTTAATCCATAG

Upstream 100 bases:

>100_bases
CTTTTATTTGTCCCTTCACTTTTGGAGAGCAAGGCCGTTTTATTAATGGTGAAAATGCGCACGGTAACTTAATGATTTCT
TCTGTTGTATTTTATGAGGC

Downstream 100 bases:

>100_bases
CTAGATTGGTAGATCTCAGCCTGCATTAACCTGCGGATCTTCTACCGAATTCTCAACCTGCTATTCTCCCACTTGTTGCG
TCCAAAGTTTGGCGTACAGC

Product: sensor histidine kinase/response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MKMDSEYTEKVNELTLELQRSIERGKQLAEENRVILAAISAFSVATNKNHIFEELKTLLNRYIQFDDFIVISKDKNGHQF
RTAIASNASFNDVVWPNGVKFQRVLNGECILLFEPCKLDEFACLMGSIDKPVYSALITGIQAQISDSVLILLGQNKGQFS
LEAKETLSRFRPLLERAISDIEHKEELQKLVEIRTRELRLAQKLAEQANQSKSNFLAMMSHELRTPLNAVLGLIDILRGE
STSYQVELLEQMENSAELLLIIINDILDLSRIESGHFSLHCHWLDLEKKLDQSLVYHRQIAQDKGVHFYVQSNKNPTLEY
YTDSARLTQILFNIVGNAIKFTKQGQVDVVLDYTKDGVVFRVKDTGIGIEKNRVEQLFTPFIQADNSITRNYGGTGLGLA
ITKHLVELMGGTIQVESEFGIGTTFTLYIPLQSRLKNIKPSTSSSQQIVNVKNHHILVVEDTKTNQMVIQLLLNKLGYDV
TIAENGLQAVELLEKNHVFDLVLMDISMPIMDGIAATKILRDKHIEIPIIALTAHTAGSDKQNCIDAGMNDIVLKPIRSK
DIMEVVKRFLNP

Sequences:

>Translated_572_residues
MKMDSEYTEKVNELTLELQRSIERGKQLAEENRVILAAISAFSVATNKNHIFEELKTLLNRYIQFDDFIVISKDKNGHQF
RTAIASNASFNDVVWPNGVKFQRVLNGECILLFEPCKLDEFACLMGSIDKPVYSALITGIQAQISDSVLILLGQNKGQFS
LEAKETLSRFRPLLERAISDIEHKEELQKLVEIRTRELRLAQKLAEQANQSKSNFLAMMSHELRTPLNAVLGLIDILRGE
STSYQVELLEQMENSAELLLIIINDILDLSRIESGHFSLHCHWLDLEKKLDQSLVYHRQIAQDKGVHFYVQSNKNPTLEY
YTDSARLTQILFNIVGNAIKFTKQGQVDVVLDYTKDGVVFRVKDTGIGIEKNRVEQLFTPFIQADNSITRNYGGTGLGLA
ITKHLVELMGGTIQVESEFGIGTTFTLYIPLQSRLKNIKPSTSSSQQIVNVKNHHILVVEDTKTNQMVIQLLLNKLGYDV
TIAENGLQAVELLEKNHVFDLVLMDISMPIMDGIAATKILRDKHIEIPIIALTAHTAGSDKQNCIDAGMNDIVLKPIRSK
DIMEVVKRFLNP
>Mature_572_residues
MKMDSEYTEKVNELTLELQRSIERGKQLAEENRVILAAISAFSVATNKNHIFEELKTLLNRYIQFDDFIVISKDKNGHQF
RTAIASNASFNDVVWPNGVKFQRVLNGECILLFEPCKLDEFACLMGSIDKPVYSALITGIQAQISDSVLILLGQNKGQFS
LEAKETLSRFRPLLERAISDIEHKEELQKLVEIRTRELRLAQKLAEQANQSKSNFLAMMSHELRTPLNAVLGLIDILRGE
STSYQVELLEQMENSAELLLIIINDILDLSRIESGHFSLHCHWLDLEKKLDQSLVYHRQIAQDKGVHFYVQSNKNPTLEY
YTDSARLTQILFNIVGNAIKFTKQGQVDVVLDYTKDGVVFRVKDTGIGIEKNRVEQLFTPFIQADNSITRNYGGTGLGLA
ITKHLVELMGGTIQVESEFGIGTTFTLYIPLQSRLKNIKPSTSSSQQIVNVKNHHILVVEDTKTNQMVIQLLLNKLGYDV
TIAENGLQAVELLEKNHVFDLVLMDISMPIMDGIAATKILRDKHIEIPIIALTAHTAGSDKQNCIDAGMNDIVLKPIRSK
DIMEVVKRFLNP

Specific function: At low cell density, in absence of autoinducer has a kinase activity, and autophosphorylates on a histidine residue. The phosphoryl group is then transferred to an aspartate residue in the response regulator domain. The phosphoryl group is transferred to

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI48994928, Length=385, Percent_Identity=35.8441558441558, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788713, Length=379, Percent_Identity=32.4538258575198, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1789149, Length=301, Percent_Identity=38.5382059800665, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI87081816, Length=400, Percent_Identity=29.25, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI145693157, Length=248, Percent_Identity=38.7096774193548, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1790436, Length=222, Percent_Identity=32.4324324324324, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1790346, Length=231, Percent_Identity=31.1688311688312, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1786600, Length=233, Percent_Identity=28.755364806867, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786912, Length=240, Percent_Identity=27.9166666666667, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788393, Length=233, Percent_Identity=26.6094420600858, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1786783, Length=227, Percent_Identity=29.0748898678414, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788549, Length=261, Percent_Identity=26.8199233716475, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788279, Length=206, Percent_Identity=28.6407766990291, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787894, Length=240, Percent_Identity=29.1666666666667, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790437, Length=118, Percent_Identity=33.8983050847458, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787229, Length=117, Percent_Identity=30.7692307692308, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87082128, Length=248, Percent_Identity=25, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=119, Percent_Identity=40.3361344537815, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6322044, Length=74, Percent_Identity=45.945945945946, Blast_Score=84, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR015387
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789
- ProDom:   PD142495 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF09308 LuxQ-periplasm; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 64562; Mature: 64562

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMDSEYTEKVNELTLELQRSIERGKQLAEENRVILAAISAFSVATNKNHIFEELKTLLN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHEECCCHHHHHHHHHHHH
RYIQFDDFIVISKDKNGHQFRTAIASNASFNDVVWPNGVKFQRVLNGECILLFEPCKLDE
HHHCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCH
FACLMGSIDKPVYSALITGIQAQISDSVLILLGQNKGQFSLEAKETLSRFRPLLERAISD
HHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHH
IEHKEELQKLVEIRTRELRLAQKLAEQANQSKSNFLAMMSHELRTPLNAVLGLIDILRGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
STSYQVELLEQMENSAELLLIIINDILDLSRIESGHFSLHCHWLDLEKKLDQSLVYHRQI
CCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHHHH
AQDKGVHFYVQSNKNPTLEYYTDSARLTQILFNIVGNAIKFTKQGQVDVVLDYTKDGVVF
HCCCCEEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHEECCCCCEEEEEEECCCCEEE
RVKDTGIGIEKNRVEQLFTPFIQADNSITRNYGGTGLGLAITKHLVELMGGTIQVESEFG
EEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCC
IGTTFTLYIPLQSRLKNIKPSTSSSQQIVNVKNHHILVVEDTKTNQMVIQLLLNKLGYDV
CCEEEEEEEEHHHHHCCCCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHCCEE
TIAENGLQAVELLEKNHVFDLVLMDISMPIMDGIAATKILRDKHIEIPIIALTAHTAGSD
EEECCCHHHHHHHHHCCCEEEEEEHHCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCC
KQNCIDAGMNDIVLKPIRSKDIMEVVKRFLNP
CHHHHHCCCCCEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKMDSEYTEKVNELTLELQRSIERGKQLAEENRVILAAISAFSVATNKNHIFEELKTLLN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHEECCCHHHHHHHHHHHH
RYIQFDDFIVISKDKNGHQFRTAIASNASFNDVVWPNGVKFQRVLNGECILLFEPCKLDE
HHHCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCH
FACLMGSIDKPVYSALITGIQAQISDSVLILLGQNKGQFSLEAKETLSRFRPLLERAISD
HHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHH
IEHKEELQKLVEIRTRELRLAQKLAEQANQSKSNFLAMMSHELRTPLNAVLGLIDILRGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
STSYQVELLEQMENSAELLLIIINDILDLSRIESGHFSLHCHWLDLEKKLDQSLVYHRQI
CCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHHHH
AQDKGVHFYVQSNKNPTLEYYTDSARLTQILFNIVGNAIKFTKQGQVDVVLDYTKDGVVF
HCCCCEEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHEECCCCCEEEEEEECCCCEEE
RVKDTGIGIEKNRVEQLFTPFIQADNSITRNYGGTGLGLAITKHLVELMGGTIQVESEFG
EEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCC
IGTTFTLYIPLQSRLKNIKPSTSSSQQIVNVKNHHILVVEDTKTNQMVIQLLLNKLGYDV
CCEEEEEEEEHHHHHCCCCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHCCEE
TIAENGLQAVELLEKNHVFDLVLMDISMPIMDGIAATKILRDKHIEIPIIALTAHTAGSD
EEECCCHHHHHHHHHCCCEEEEEEHHCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCC
KQNCIDAGMNDIVLKPIRSKDIMEVVKRFLNP
CHHHHHCCCCCEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]