Click here to switch to the map view.

The map label for this gene is potD-2 [H]

Identifier: 227081597

GI number: 227081597

Start: 1480483

End: 1481583

Strand: Reverse

Name: potD-2 [H]

Synonym: VCM66_1381

Alternate gene names: 227081597

Gene position: 1481583-1480483 (Counterclockwise)

Preceding gene: 227081598

Following gene: 227081596

Centisome position: 51.22

GC content: 44.96

Gene sequence:

>1101_bases
ATGACGGAAGTCATTCTGTTTTTCTTTGCAACGCCCAAAAGGGCAACGTTTAGTTTGGAGCTAACGTCGATGAAAAAACT
GGCTACTCTTTTAGCAGGTAGCGCATGTGCGCTTTCTCTGTTGTCTGGCACAGCGGCAGCGGAAGATAAGGAACTGGTGT
TTATGAACTGGGGACCGTATATCAATAGCGGTATCCTAGAACAGTTCACCAAGGAAACGGGAATCAAAGTGATTTACTCA
ACTTATGAGTCAAATGAAACTTTGTATGCAAAACTCAAAACGCACAATCAAGGTTATGATCTGGTAGTTCCTTCTACCTA
CTTCGTGGCAAAAATGCGTGATGAAGGGATGCTGCAAAAAATTGACAAATCTAAGCTGAGCAACTTCAAAAACCTAGATT
CCAACTACCTAGATAAACCTTACGACCCAAACAACGACTACTCAATTCCACACGTTGTAGCAATTACCGGTCTCGCGGTT
AATACCGACATGTATGATCCTAATGACTTCCAAAGTTGGGGCGATCTATGGAAGCCTGAGCTAAAAGGTCAACTGATGTT
GATGGATGATACGCGTGAAGTATTCCATATCGCTCTGCGTAAACTGGGTTACTCAGGTAACTCAACGGACGACAAGCAGA
TTGATGAAGCGTATGCCGAGCTACAAAAGCTGATGCCAAACGTACTGGTTTTCAACTCTGATAACCCTGCAGCTCCTTAC
CTTTCAGGTGAAGTAGGTCTTGGTATGCTGTGGAACGGCTCAGCAGCGGCGGCACAGAAAGAAGGTTTACCAATCAAGCT
AGTCTTCCCGAAAGAAGGCGGTATCGGTTGGGTTGATAACTTCGCCATCTCTTCTGGTGCCAAAAACGTCGATGCAGCAC
ACAAGATGATCGATTTCTTGTTACGTCCTGAAATTGCAGAGCAAATTTCACGCGACACAGGTTATCTGACTGGCGTTAAA
GCCTCAAACGACAAATTCCAAGATGTCGCGCCACTGTTCCCATCTCAAGAAGATCTGGACCGTGTTGAGTGGCAATCTGC
CGTTGGCGATAAGACAGTGAAGTATGAAGAGTACTTCATGAAGTTGAAAGCTGGCCAATAA

Upstream 100 bases:

>100_bases
CATGCTTGTGGTTTCTTTGGTACTTGTTGTCGCTTCACAGTTGTTAGCAAGAGAAAAAGTGAAGTAAAATCCACGTCCGT
TTGGAAACACGCGAAACAGA

Downstream 100 bases:

>100_bases
GGTCTAAGAAAAGCATCAAATAGGCAGCCATTGCTGCCTATTTTTTTTTATCTTGTTATACTCCCCTCTCCAACGCCTAG
TTAGGTTCTTTCTATACACT

Product: spermidine/putrescine ABC transporter, periplasmic spermidine/putrescine-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MTEVILFFFATPKRATFSLELTSMKKLATLLAGSACALSLLSGTAAAEDKELVFMNWGPYINSGILEQFTKETGIKVIYS
TYESNETLYAKLKTHNQGYDLVVPSTYFVAKMRDEGMLQKIDKSKLSNFKNLDSNYLDKPYDPNNDYSIPHVVAITGLAV
NTDMYDPNDFQSWGDLWKPELKGQLMLMDDTREVFHIALRKLGYSGNSTDDKQIDEAYAELQKLMPNVLVFNSDNPAAPY
LSGEVGLGMLWNGSAAAAQKEGLPIKLVFPKEGGIGWVDNFAISSGAKNVDAAHKMIDFLLRPEIAEQISRDTGYLTGVK
ASNDKFQDVAPLFPSQEDLDRVEWQSAVGDKTVKYEEYFMKLKAGQ

Sequences:

>Translated_366_residues
MTEVILFFFATPKRATFSLELTSMKKLATLLAGSACALSLLSGTAAAEDKELVFMNWGPYINSGILEQFTKETGIKVIYS
TYESNETLYAKLKTHNQGYDLVVPSTYFVAKMRDEGMLQKIDKSKLSNFKNLDSNYLDKPYDPNNDYSIPHVVAITGLAV
NTDMYDPNDFQSWGDLWKPELKGQLMLMDDTREVFHIALRKLGYSGNSTDDKQIDEAYAELQKLMPNVLVFNSDNPAAPY
LSGEVGLGMLWNGSAAAAQKEGLPIKLVFPKEGGIGWVDNFAISSGAKNVDAAHKMIDFLLRPEIAEQISRDTGYLTGVK
ASNDKFQDVAPLFPSQEDLDRVEWQSAVGDKTVKYEEYFMKLKAGQ
>Mature_365_residues
TEVILFFFATPKRATFSLELTSMKKLATLLAGSACALSLLSGTAAAEDKELVFMNWGPYINSGILEQFTKETGIKVIYST
YESNETLYAKLKTHNQGYDLVVPSTYFVAKMRDEGMLQKIDKSKLSNFKNLDSNYLDKPYDPNNDYSIPHVVAITGLAVN
TDMYDPNDFQSWGDLWKPELKGQLMLMDDTREVFHIALRKLGYSGNSTDDKQIDEAYAELQKLMPNVLVFNSDNPAAPYL
SGEVGLGMLWNGSAAAAQKEGLPIKLVFPKEGGIGWVDNFAISSGAKNVDAAHKMIDFLLRPEIAEQISRDTGYLTGVKA
SNDKFQDVAPLFPSQEDLDRVEWQSAVGDKTVKYEEYFMKLKAGQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=348, Percent_Identity=57.183908045977, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI1787078, Length=323, Percent_Identity=35.9133126934984, Blast_Score=172, Evalue=2e-44,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40782; Mature: 40650

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVILFFFATPKRATFSLELTSMKKLATLLAGSACALSLLSGTAAAEDKELVFMNWGPY
CCEEEEEEEECCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEECCCC
INSGILEQFTKETGIKVIYSTYESNETLYAKLKTHNQGYDLVVPSTYFVAKMRDEGMLQK
CCHHHHHHHHHHCCCEEEEEEECCCCEEEEEEEECCCCCEEEECCCEEEEEECCCHHHHH
IDKSKLSNFKNLDSNYLDKPYDPNNDYSIPHVVAITGLAVNTDMYDPNDFQSWGDLWKPE
HHHHHHHHHHCCCHHCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCHHHHHHCCCCC
LKGQLMLMDDTREVFHIALRKLGYSGNSTDDKQIDEAYAELQKLMPNVLVFNSDNPAAPY
CCCEEEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCE
LSGEVGLGMLWNGSAAAAQKEGLPIKLVFPKEGGIGWVDNFAISSGAKNVDAAHKMIDFL
ECCCCCEEEEECCCCHHHHHCCCCEEEEEECCCCCCCHHCHHHCCCCCCHHHHHHHHHHH
LRPEIAEQISRDTGYLTGVKASNDKFQDVAPLFPSQEDLDRVEWQSAVGDKTVKYEEYFM
HCHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHH
KLKAGQ
EECCCC
>Mature Secondary Structure 
TEVILFFFATPKRATFSLELTSMKKLATLLAGSACALSLLSGTAAAEDKELVFMNWGPY
CEEEEEEEECCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEECCCC
INSGILEQFTKETGIKVIYSTYESNETLYAKLKTHNQGYDLVVPSTYFVAKMRDEGMLQK
CCHHHHHHHHHHCCCEEEEEEECCCCEEEEEEEECCCCCEEEECCCEEEEEECCCHHHHH
IDKSKLSNFKNLDSNYLDKPYDPNNDYSIPHVVAITGLAVNTDMYDPNDFQSWGDLWKPE
HHHHHHHHHHCCCHHCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCHHHHHHCCCCC
LKGQLMLMDDTREVFHIALRKLGYSGNSTDDKQIDEAYAELQKLMPNVLVFNSDNPAAPY
CCCEEEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCE
LSGEVGLGMLWNGSAAAAQKEGLPIKLVFPKEGGIGWVDNFAISSGAKNVDAAHKMIDFL
ECCCCCEEEEECCCCHHHHHCCCCEEEEEECCCCCCCHHCHHHCCCCCCHHHHHHHHHHH
LRPEIAEQISRDTGYLTGVKASNDKFQDVAPLFPSQEDLDRVEWQSAVGDKTVKYEEYFM
HCHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHH
KLKAGQ
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]