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The map label for this gene is yaaJ [C]

Identifier: 227081593

GI number: 227081593

Start: 1476399

End: 1477775

Strand: Reverse

Name: yaaJ [C]

Synonym: VCM66_1377

Alternate gene names: 227081593

Gene position: 1477775-1476399 (Counterclockwise)

Preceding gene: 227081596

Following gene: 227081585

Centisome position: 51.09

GC content: 49.89

Gene sequence:

>1377_bases
ATGAATAACCTGCAATCTTTTCTGCAAACTGTTGATAGTTTAGTGTGGGGTCCACCACTGTTAATTCTTCTGGTCGGCAC
TGGTGTCTATTTCACCTTCCGCTTGGGCTTACTGCAATTTCGACGCCTGCCGACGGCTTTAGCTATGGTATTTGGCCGCG
AAAAATCTTCAGACAAACAAGGTGATGTATCAAGCTTCGCCGCATTGTGTACTGCTCTTTCTGCCACCATTGGTACTGGA
AACATCGTAGGCGTTGCGACCGCAATCAAGCTCGGCGGCCCAGGTGCCCTGTTCTGGATGTGGCTCGCCGCCCTGTTTGG
CATGGCAACCAAATACGCAGAATGCTTACTCGCGGTGAAATATCGCCAAATCGATGACAAAGGACAAATGGTCGGCGGGC
CAATGTACTACCTGCGTGATGGTGTGAGTTCAAAAACACTGGCAGTATTATTTGCTGTGTTTGCCGTAGGGGTTGCCTGC
TTCGGGATTGGCACTTTCCCACAAGTGAACGCGATCTTAGATGCCACTCAAATCTCTTTTGGTGTCCCTCGTGAAGCCTC
TGCGGTGGTACTGACCGTATTGGTTGCCATTGTCACTATCGGTGGTATTCAATCCATCGCTAAAGTGGCAGGAAAAGTGG
TTCCTGCGATGGCGCTGTTTTATATCGTCGCCTGCTTAAGTGTGATTGTTACCAATGCGGACAAACTCGCAGACGCCGTG
GAATTGGTGCTGGTATCCGCGTTCACCTCAACTGCTGCAACTGGTGGCTTCTTAGGTGCGAGCATTATGCTCGCGATTCA
ATCGGGTATTGCCCGCGGCGTGTTCTCCAATGAATCTGGCTTAGGCAGTGCGCCTATGGCGGCGGCGGCGGCGAAAACTG
ACTCCTGCGTTGAGCAAGGCCTTATCTCTATGACGGGGACTTTCTTTGACACCATTATCATCTGTACGATGACTGGCTTG
GCTCTCATTCTAACCGGCGCTTGGCAAAGCGATCTTTCCGGTGCTGCGATGACCACCTACGCCTTTGCCACAGGCTTAAA
TGCACAAACTATCGGCCCGATGCTGGTTTCCATTGGTTTGATGTTCTTTGCCTTTACGACCATTCTGGGTTGGAACTACT
ACGGCGAACGCTGCATGGTGTTCTTATTCGGAACCAAAGCGGTATTACCTTACAAAATCGTCTTTATTGGTTTGATTGCC
TCAGGCGCATTCCTACACCTCGATTTGATTTGGATTATCGCCGACATTGTAAATGGTTTAATGGCCATTCCGAACCTTAT
TGGCCTTGTTGCCTTACGCCATGTGGTCGTAGAAGAAACGAAGCAATATTTTGCCGCGCGTTATCAATACTCCGAAGCCG
AAGCCCAAGTTCAATAA

Upstream 100 bases:

>100_bases
GAGTGAAAGGCCAATCTTTTAGTGAGCTCGCTAGAGCTCTGCTGTGCATTTTTCGCACCCTTTCTCTCTTCTCCTTATGT
TTATTTTTTAAGGGGAAATC

Downstream 100 bases:

>100_bases
CACCTAGTCGGTCTAAGCACAAAGAAAAAGCGCCCAACGGCGCTTTTTCTACTTTTAAACCTTTATTACACGCTAGGCTT
GAGGTTTCTCTAAGAGCAGT

Product: sodium/alanine symporter

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MNNLQSFLQTVDSLVWGPPLLILLVGTGVYFTFRLGLLQFRRLPTALAMVFGREKSSDKQGDVSSFAALCTALSATIGTG
NIVGVATAIKLGGPGALFWMWLAALFGMATKYAECLLAVKYRQIDDKGQMVGGPMYYLRDGVSSKTLAVLFAVFAVGVAC
FGIGTFPQVNAILDATQISFGVPREASAVVLTVLVAIVTIGGIQSIAKVAGKVVPAMALFYIVACLSVIVTNADKLADAV
ELVLVSAFTSTAATGGFLGASIMLAIQSGIARGVFSNESGLGSAPMAAAAAKTDSCVEQGLISMTGTFFDTIIICTMTGL
ALILTGAWQSDLSGAAMTTYAFATGLNAQTIGPMLVSIGLMFFAFTTILGWNYYGERCMVFLFGTKAVLPYKIVFIGLIA
SGAFLHLDLIWIIADIVNGLMAIPNLIGLVALRHVVVEETKQYFAARYQYSEAEAQVQ

Sequences:

>Translated_458_residues
MNNLQSFLQTVDSLVWGPPLLILLVGTGVYFTFRLGLLQFRRLPTALAMVFGREKSSDKQGDVSSFAALCTALSATIGTG
NIVGVATAIKLGGPGALFWMWLAALFGMATKYAECLLAVKYRQIDDKGQMVGGPMYYLRDGVSSKTLAVLFAVFAVGVAC
FGIGTFPQVNAILDATQISFGVPREASAVVLTVLVAIVTIGGIQSIAKVAGKVVPAMALFYIVACLSVIVTNADKLADAV
ELVLVSAFTSTAATGGFLGASIMLAIQSGIARGVFSNESGLGSAPMAAAAAKTDSCVEQGLISMTGTFFDTIIICTMTGL
ALILTGAWQSDLSGAAMTTYAFATGLNAQTIGPMLVSIGLMFFAFTTILGWNYYGERCMVFLFGTKAVLPYKIVFIGLIA
SGAFLHLDLIWIIADIVNGLMAIPNLIGLVALRHVVVEETKQYFAARYQYSEAEAQVQ
>Mature_458_residues
MNNLQSFLQTVDSLVWGPPLLILLVGTGVYFTFRLGLLQFRRLPTALAMVFGREKSSDKQGDVSSFAALCTALSATIGTG
NIVGVATAIKLGGPGALFWMWLAALFGMATKYAECLLAVKYRQIDDKGQMVGGPMYYLRDGVSSKTLAVLFAVFAVGVAC
FGIGTFPQVNAILDATQISFGVPREASAVVLTVLVAIVTIGGIQSIAKVAGKVVPAMALFYIVACLSVIVTNADKLADAV
ELVLVSAFTSTAATGGFLGASIMLAIQSGIARGVFSNESGLGSAPMAAAAAKTDSCVEQGLISMTGTFFDTIIICTMTGL
ALILTGAWQSDLSGAAMTTYAFATGLNAQTIGPMLVSIGLMFFAFTTILGWNYYGERCMVFLFGTKAVLPYKIVFIGLIA
SGAFLHLDLIWIIADIVNGLMAIPNLIGLVALRHVVVEETKQYFAARYQYSEAEAQVQ

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=454, Percent_Identity=35.0220264317181, Blast_Score=247, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 48257; Mature: 48257

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]