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The map label for this gene is lplA

Identifier: 227081559

GI number: 227081559

Start: 1436596

End: 1437612

Strand: Direct

Name: lplA

Synonym: VCM66_1343

Alternate gene names: 227081559

Gene position: 1436596-1437612 (Clockwise)

Preceding gene: 227081558

Following gene: 227081560

Centisome position: 49.67

GC content: 50.93

Gene sequence:

>1017_bases
ATGACTAAGACTCGCATTCTGCTCTCTGATTCTACGGACCCTTGGTTTAACCTCGCCGTCGAAGACACGATTTTTCGTTC
TATGCCCGCCGATCAGCGTGTGCTGTTTTTGTGGCGTAACGCCGATACCGTCGTGATTGGTCGTGCGCAAAACCCGTGGC
GCGAATGCAAAACGGATCGTATGGAGCAGGACAAAGTGAAACTCGCACGTCGTCAGACTGGGGGCGGGGCAGTTTTCCAT
GATTTGGGCAATACCAACTTCACCTTTATGGCGGGTAAACCGGAGTACGATAAAGAGGTCTCAACCAAGATTGTGTTGGC
GGGGCTGCAAAAGTTGGGGATTCATGGGGTCGCCAACGGGCGCAATGATCTGGTACTGGAAGATGAACAGGGTATTCGTA
AGTTTTCCGGTTCTGCTTATCGCGAAACGCTGGATCGCGGATTTCACCACGGCACGCTGCTGCTGAGTGCCGATCTCAAC
CGTTTAGCCGATTACCTCAATCCCGATCTTAAAAAGCTGCAAGCGAAAGGCATAACCTCGGTGAAATCGCGAGTCATCAA
CCTCAACACGGTTAAAGCGGATATTGAGCACCAACAAGTGTGTGAAGCGATCATGCAAGCCTATTGCGAACATTACCAAC
AGCAGGTTGAGCCTGAGTTGATCTCGCCGCAGTCGTTTTTTGATTTGCCGGGATTTGAGCAAAAATTCGCTCAGCAAAGC
AGTTGGGATTGGAACTTCGGACAAACGCCACCTTTTACTCACCACATGGATGAACGATTTAGCTGGGGTGGCGTCGAGGT
GTATCTTGAAGTTGAGCGCGGCACGATTGTGCAAGCCACGATTTTCAGCGACATGCTGGACCCTTATCCGATGGAGCAAT
TGGCGCTGCGTTTAAGCGGGCTCACCTACAACAAAACGGCGCTTGAACCGTGCCTTGCCCAATTGATGCAGGAATTGCCA
CAATACCAATTGCCTCTAGAAGAGTTTCAGCGCTGGTTCATCGACCAGATTGATTGA

Upstream 100 bases:

>100_bases
CTTTTTTGTGTGGCGAATTTATTGAAAGCAAGGGGTTTGGCTATCTGCGCAGAATTGCGTAACATGGCGCATCTTTTCTA
TTGCTTGGTACGCAACAACC

Downstream 100 bases:

>100_bases
GCCTCACCTGTACGTTTTTGTAACCGCGCTCCTGCTTATCATTTCCTATTCTCGATTCCCTCCTCACTCAGAGGAGGGGC
TCACCAAGCGTATTCACAAA

Product: lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MTKTRILLSDSTDPWFNLAVEDTIFRSMPADQRVLFLWRNADTVVIGRAQNPWRECKTDRMEQDKVKLARRQTGGGAVFH
DLGNTNFTFMAGKPEYDKEVSTKIVLAGLQKLGIHGVANGRNDLVLEDEQGIRKFSGSAYRETLDRGFHHGTLLLSADLN
RLADYLNPDLKKLQAKGITSVKSRVINLNTVKADIEHQQVCEAIMQAYCEHYQQQVEPELISPQSFFDLPGFEQKFAQQS
SWDWNFGQTPPFTHHMDERFSWGGVEVYLEVERGTIVQATIFSDMLDPYPMEQLALRLSGLTYNKTALEPCLAQLMQELP
QYQLPLEEFQRWFIDQID

Sequences:

>Translated_338_residues
MTKTRILLSDSTDPWFNLAVEDTIFRSMPADQRVLFLWRNADTVVIGRAQNPWRECKTDRMEQDKVKLARRQTGGGAVFH
DLGNTNFTFMAGKPEYDKEVSTKIVLAGLQKLGIHGVANGRNDLVLEDEQGIRKFSGSAYRETLDRGFHHGTLLLSADLN
RLADYLNPDLKKLQAKGITSVKSRVINLNTVKADIEHQQVCEAIMQAYCEHYQQQVEPELISPQSFFDLPGFEQKFAQQS
SWDWNFGQTPPFTHHMDERFSWGGVEVYLEVERGTIVQATIFSDMLDPYPMEQLALRLSGLTYNKTALEPCLAQLMQELP
QYQLPLEEFQRWFIDQID
>Mature_337_residues
TKTRILLSDSTDPWFNLAVEDTIFRSMPADQRVLFLWRNADTVVIGRAQNPWRECKTDRMEQDKVKLARRQTGGGAVFHD
LGNTNFTFMAGKPEYDKEVSTKIVLAGLQKLGIHGVANGRNDLVLEDEQGIRKFSGSAYRETLDRGFHHGTLLLSADLNR
LADYLNPDLKKLQAKGITSVKSRVINLNTVKADIEHQQVCEAIMQAYCEHYQQQVEPELISPQSFFDLPGFEQKFAQQSS
WDWNFGQTPPFTHHMDERFSWGGVEVYLEVERGTIVQATIFSDMLDPYPMEQLALRLSGLTYNKTALEPCLAQLMQELPQ
YQLPLEEFQRWFIDQID

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family

Homologues:

Organism=Homo sapiens, GI7706252, Length=278, Percent_Identity=33.8129496402878, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI21729884, Length=278, Percent_Identity=33.8129496402878, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI21729882, Length=278, Percent_Identity=33.8129496402878, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI21729880, Length=278, Percent_Identity=33.8129496402878, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI21729878, Length=278, Percent_Identity=33.8129496402878, Blast_Score=159, Evalue=2e-39,
Organism=Escherichia coli, GI1790846, Length=337, Percent_Identity=62.0178041543027, Blast_Score=447, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI71981909, Length=263, Percent_Identity=29.277566539924, Blast_Score=124, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6322415, Length=196, Percent_Identity=32.1428571428571, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI28573464, Length=336, Percent_Identity=31.547619047619, Blast_Score=141, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPLA_VIBC3 (A5F8E4)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F8E4
- SMR:   A5F8E4
- STRING:   A5F8E4
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1000
- eggNOG:   COG0095
- HOGENOM:   HBG715377
- OMA:   YEQSHLE
- ProtClustDB:   PRK03822
- BioCyc:   VCHO345073:VC0395_A1000-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01602
- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562
- Gene3D:   G3DSA:3.30.390.50
- TIGRFAMs:   TIGR00545

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C

EC number: =2.7.7.63

Molecular weight: Translated: 38797; Mature: 38666

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: BINDING 71-71 BINDING 134-134 BINDING 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTRILLSDSTDPWFNLAVEDTIFRSMPADQRVLFLWRNADTVVIGRAQNPWRECKTDR
CCCCEEEEECCCCCCEEEEHHHHHHHCCCCCCEEEEEEECCCEEEEECCCCCHHHHHHCC
MEQDKVKLARRQTGGGAVFHDLGNTNFTFMAGKPEYDKEVSTKIVLAGLQKLGIHGVANG
CHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCEEECCC
RNDLVLEDEQGIRKFSGSAYRETLDRGFHHGTLLLSADLNRLADYLNPDLKKLQAKGITS
CCCEEEECHHHHHHHCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCHHHHHHHHCCHHH
VKSRVINLNTVKADIEHQQVCEAIMQAYCEHYQQQVEPELISPQSFFDLPGFEQKFAQQS
HHHHEECEEHEEHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCHHHHHHHCC
SWDWNFGQTPPFTHHMDERFSWGGVEVYLEVERGTIVQATIFSDMLDPYPMEQLALRLSG
CCCCCCCCCCCCCCCHHHHCCCCCEEEEEEECCCCEEEHHHHHHHCCCCCHHHHHHHHCC
LTYNKTALEPCLAQLMQELPQYQLPLEEFQRWFIDQID
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TKTRILLSDSTDPWFNLAVEDTIFRSMPADQRVLFLWRNADTVVIGRAQNPWRECKTDR
CCCEEEEECCCCCCEEEEHHHHHHHCCCCCCEEEEEEECCCEEEEECCCCCHHHHHHCC
MEQDKVKLARRQTGGGAVFHDLGNTNFTFMAGKPEYDKEVSTKIVLAGLQKLGIHGVANG
CHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCEEECCC
RNDLVLEDEQGIRKFSGSAYRETLDRGFHHGTLLLSADLNRLADYLNPDLKKLQAKGITS
CCCEEEECHHHHHHHCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCHHHHHHHHCCHHH
VKSRVINLNTVKADIEHQQVCEAIMQAYCEHYQQQVEPELISPQSFFDLPGFEQKFAQQS
HHHHEECEEHEEHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCHHHHHHHCC
SWDWNFGQTPPFTHHMDERFSWGGVEVYLEVERGTIVQATIFSDMLDPYPMEQLALRLSG
CCCCCCCCCCCCCCCHHHHCCCCCEEEEEEECCCCEEEHHHHHHHCCCCCHHHHHHHHCC
LTYNKTALEPCLAQLMQELPQYQLPLEEFQRWFIDQID
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA