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The map label for this gene is exsB

Identifier: 227081537

GI number: 227081537

Start: 1412933

End: 1413628

Strand: Direct

Name: exsB

Synonym: VCM66_1321

Alternate gene names: 227081537

Gene position: 1412933-1413628 (Clockwise)

Preceding gene: 227081536

Following gene: 227081539

Centisome position: 48.85

GC content: 48.71

Gene sequence:

>696_bases
ATGAAAAAAGCCGTCGTCGTGTTCAGTGGTGGGCAAGATTCAACCACCTGTTTAGTTCAGGCGTTAAAAGAGTTTGATGA
AGTGCATGCGATCACCTTTGATTATGGCCAGCGCCATAAATTAGAGATAGAAGTGGCCCAGCAATTGGCGAAGCAGCTTG
GCGTGACTGCGCATAAAGTCATGGACGTGAGTTTACTCAATGAGCTGGCCATCAGCTCGCTGACGCGTGATGACATTCCG
GTTTCTCATGAGCTACAAGCCAATGGACTGCCGAATTCTTTTGTTCCGGGACGCAATATTCTGTTTTTAACTTTGGCGGG
TATCTATGCTTACCAAATCGGCGCGACCACGGTCATTACTGGGGTTTGTGAGACGGATTTTTCCGGTTATCCCGATTGCC
GTGATGAATTCGTACAAGCGATGAACCAAGCACTCGCGAAAGGCATGGATCTGCCTTTAATGATTCGCACTCCATTGATG
TGGCTGAATAAAGCTGAGACGTGGGCATTGGCCGATCAACTGGGAGCATTGGATCTGGTTCGTCACCAAACGCTCACTTG
TTATAACGGGTTGATAGGTGATGGCTGTGGTGAATGCCCAGCCTGTGGTTTACGTCAGGCTGGGCTTAAAGCGTATCTCG
ATAATCGCGATCTGATAATGAGTGCGCTTAAGAGCAAGCAGTCAGCAGCGCATTAG

Upstream 100 bases:

>100_bases
GCGTGCGACAGAGTTGTGCATTCGCACCTGTATTGCTCGCAATTGGCGTTTATCTATCCAAACCCATAAATATCTCAACA
TTGCTTAAAGGGAGCGCATC

Downstream 100 bases:

>100_bases
TGGGATCGGCGGCATCGATTAAGCCGCCATTGCCGCTCTGTTTGGCGATGCTTAGAGCGCTATCCAGCTTAGGATAAAAG
TGCGTAAAACTCGCCAAACC

Product: queuosine biosynthesis protein QueC

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine synthase; PreQ(0) synthase; Queuosine biosynthesis protein queC

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MKKAVVVFSGGQDSTTCLVQALKEFDEVHAITFDYGQRHKLEIEVAQQLAKQLGVTAHKVMDVSLLNELAISSLTRDDIP
VSHELQANGLPNSFVPGRNILFLTLAGIYAYQIGATTVITGVCETDFSGYPDCRDEFVQAMNQALAKGMDLPLMIRTPLM
WLNKAETWALADQLGALDLVRHQTLTCYNGLIGDGCGECPACGLRQAGLKAYLDNRDLIMSALKSKQSAAH

Sequences:

>Translated_231_residues
MKKAVVVFSGGQDSTTCLVQALKEFDEVHAITFDYGQRHKLEIEVAQQLAKQLGVTAHKVMDVSLLNELAISSLTRDDIP
VSHELQANGLPNSFVPGRNILFLTLAGIYAYQIGATTVITGVCETDFSGYPDCRDEFVQAMNQALAKGMDLPLMIRTPLM
WLNKAETWALADQLGALDLVRHQTLTCYNGLIGDGCGECPACGLRQAGLKAYLDNRDLIMSALKSKQSAAH
>Mature_231_residues
MKKAVVVFSGGQDSTTCLVQALKEFDEVHAITFDYGQRHKLEIEVAQQLAKQLGVTAHKVMDVSLLNELAISSLTRDDIP
VSHELQANGLPNSFVPGRNILFLTLAGIYAYQIGATTVITGVCETDFSGYPDCRDEFVQAMNQALAKGMDLPLMIRTPLM
WLNKAETWALADQLGALDLVRHQTLTCYNGLIGDGCGECPACGLRQAGLKAYLDNRDLIMSALKSKQSAAH

Specific function: Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0))

COG id: COG0603

COG function: function code R; Predicted PP-loop superfamily ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queC family

Homologues:

Organism=Escherichia coli, GI1786648, Length=227, Percent_Identity=69.1629955947137, Blast_Score=339, Evalue=8e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEC_VIBC3 (A5F8G4)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F8G4
- SMR:   A5F8G4
- STRING:   A5F8G4
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0980
- eggNOG:   COG0603
- HOGENOM:   HBG553284
- OMA:   CETDYSG
- ProtClustDB:   PRK11106
- BioCyc:   VCHO345073:VC0395_A0980-MONOMER
- HAMAP:   MF_01633_B
- InterPro:   IPR018317
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF006293
- TIGRFAMs:   TIGR00364

Pfam domain/function: PF06508 ExsB

EC number: NA

Molecular weight: Translated: 25159; Mature: 25159

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAVVVFSGGQDSTTCLVQALKEFDEVHAITFDYGQRHKLEIEVAQQLAKQLGVTAHKV
CCCEEEEEECCCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEHHHHHHHHHHHCCHHHHH
MDVSLLNELAISSLTRDDIPVSHELQANGLPNSFVPGRNILFLTLAGIYAYQIGATTVIT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHH
GVCETDFSGYPDCRDEFVQAMNQALAKGMDLPLMIRTPLMWLNKAETWALADQLGALDLV
HHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHCCCHHHHHHHHHHHHHHH
RHQTLTCYNGLIGDGCGECPACGLRQAGLKAYLDNRDLIMSALKSKQSAAH
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKAVVVFSGGQDSTTCLVQALKEFDEVHAITFDYGQRHKLEIEVAQQLAKQLGVTAHKV
CCCEEEEEECCCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEHHHHHHHHHHHCCHHHHH
MDVSLLNELAISSLTRDDIPVSHELQANGLPNSFVPGRNILFLTLAGIYAYQIGATTVIT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHH
GVCETDFSGYPDCRDEFVQAMNQALAKGMDLPLMIRTPLMWLNKAETWALADQLGALDLV
HHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHCCCHHHHHHHHHHHHHHH
RHQTLTCYNGLIGDGCGECPACGLRQAGLKAYLDNRDLIMSALKSKQSAAH
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA