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The map label for this gene is mii [H]

Identifier: 227081510

GI number: 227081510

Start: 1382095

End: 1383297

Strand: Direct

Name: mii [H]

Synonym: VCM66_1294

Alternate gene names: 227081510

Gene position: 1382095-1383297 (Clockwise)

Preceding gene: 227081509

Following gene: 227081511

Centisome position: 47.78

GC content: 54.53

Gene sequence:

>1203_bases
ATGAACGAGACAGTGCCACAAATTCGTATTCCAGCCACCTACATGCGCGGCGGAACCAGCAAAGGGGTGTTTTTCCGTCT
GAACGATTTGCCGCTTGAAGCGCAGCTTCCCGGTGCGGCCCGTGATGCTCTGTTGTTACGAGTGATTGGTAGCCCAGATC
CTTACGGTAAACAGATTGATGGCATGGGCGGCGCGACATCGAGCACCAGCAAAACGGTGATTGTCAGTGCCAGTTCGCGC
ACTGATCACGATGTGGATTATCTGTTCGGGCAAGTGGCGATAGATAAACCGTTTGTCGATTGGAGCGGAAACTGCGGCAA
CCTTTCCGCTGCGGTGGGGCCCTTTGCGATCCATGCTGGGCTGATTCCTGCTGAGTGCATTCCGCACAATGGTGTGGTGG
CGGTGAAAGTGTGGCAAGTCAACATCAGCAAAACCATTGTGGTGCATGTGCCGATAGTCAATGGCGCAGTGCAGGAGTGC
GGTGAATTTGAACTCGATGGCGTGACCTTTGCGGCTGCCGAAGTGCAGGTCGATTTTGTGGATCCTGCGGATGACAGCGG
CGCTATGTTCCCAACTGGTAACGTGATGGATGATTTACAAGTGCCGGGTATCGGCACGTTAAATGCGACTTTCATCAACT
CGGGCATTCCAACCATTTTTGTCGATGCCGAAGCGCTTGGCTATCAAGGCACTGAGTTGCAAGAGGCCATCAACAGTGAT
CCACAAGCCTTGGCCAAGTTTGAAACGCTACGCGCTTATGGCGCACTGAAAATGGGTCTGATTGAGCGCATTGAAGAAGC
GCAAAACCGTCAGCACACGCCAAAAATTGCTTTTGTCAGTGGCGCGAAAAGTTACTTAGCTTCAAGCGGTAAGCGAGTAG
AAGCGGATTCCGTGGATCTGTTGGTGCGCGCGCTCTCGATGGGCAAACTGCATCACGCCATGATGGGCACGGCCGCCGTG
GCGATTGCTTCTGCTGCCGCCGTTCCGGGCACGTTGGTCAATTTGGCTGCCGGGGGCGGCGAGCGTACGTCAGTCACCTT
TGGTCATCCCTCCGGCACGTTAAAAGTCGGAGCGAAAGCCAAGCAAGGTAAACAAGGCTGGCAAATTGAGCGCGCGATCA
TGAGCCGCAGCGCCAGAATTCTAATGGAAGGATGGGTACGCGTTCCCGCAAATTACGCTGCCCTCAGTGAGAGCAAGGAG
TAA

Upstream 100 bases:

>100_bases
TGACGTGTCGTTTGGACACGGCCGATGAAGTGCACGTATATCAAGCGGGTGGCGTGTTGCAACGCTTTGCGCAAGACTTT
TTGGCGCAGTAGGAGGCAGC

Downstream 100 bases:

>100_bases
TGCGATGTCTGCCTATCAAGAGCAATATCAATGGGCGAAAGAGCAGCCCGAAGCCTTCTGGCGTAAACAAGCGCAGCAGA
TCGACTGGTTTCAACCGCCG

Product: hypothetical protein

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MNETVPQIRIPATYMRGGTSKGVFFRLNDLPLEAQLPGAARDALLLRVIGSPDPYGKQIDGMGGATSSTSKTVIVSASSR
TDHDVDYLFGQVAIDKPFVDWSGNCGNLSAAVGPFAIHAGLIPAECIPHNGVVAVKVWQVNISKTIVVHVPIVNGAVQEC
GEFELDGVTFAAAEVQVDFVDPADDSGAMFPTGNVMDDLQVPGIGTLNATFINSGIPTIFVDAEALGYQGTELQEAINSD
PQALAKFETLRAYGALKMGLIERIEEAQNRQHTPKIAFVSGAKSYLASSGKRVEADSVDLLVRALSMGKLHHAMMGTAAV
AIASAAAVPGTLVNLAAGGGERTSVTFGHPSGTLKVGAKAKQGKQGWQIERAIMSRSARILMEGWVRVPANYAALSESKE

Sequences:

>Translated_400_residues
MNETVPQIRIPATYMRGGTSKGVFFRLNDLPLEAQLPGAARDALLLRVIGSPDPYGKQIDGMGGATSSTSKTVIVSASSR
TDHDVDYLFGQVAIDKPFVDWSGNCGNLSAAVGPFAIHAGLIPAECIPHNGVVAVKVWQVNISKTIVVHVPIVNGAVQEC
GEFELDGVTFAAAEVQVDFVDPADDSGAMFPTGNVMDDLQVPGIGTLNATFINSGIPTIFVDAEALGYQGTELQEAINSD
PQALAKFETLRAYGALKMGLIERIEEAQNRQHTPKIAFVSGAKSYLASSGKRVEADSVDLLVRALSMGKLHHAMMGTAAV
AIASAAAVPGTLVNLAAGGGERTSVTFGHPSGTLKVGAKAKQGKQGWQIERAIMSRSARILMEGWVRVPANYAALSESKE
>Mature_400_residues
MNETVPQIRIPATYMRGGTSKGVFFRLNDLPLEAQLPGAARDALLLRVIGSPDPYGKQIDGMGGATSSTSKTVIVSASSR
TDHDVDYLFGQVAIDKPFVDWSGNCGNLSAAVGPFAIHAGLIPAECIPHNGVVAVKVWQVNISKTIVVHVPIVNGAVQEC
GEFELDGVTFAAAEVQVDFVDPADDSGAMFPTGNVMDDLQVPGIGTLNATFINSGIPTIFVDAEALGYQGTELQEAINSD
PQALAKFETLRAYGALKMGLIERIEEAQNRQHTPKIAFVSGAKSYLASSGKRVEADSVDLLVRALSMGKLHHAMMGTAAV
AIASAAAVPGTLVNLAAGGGERTSVTFGHPSGTLKVGAKAKQGKQGWQIERAIMSRSARILMEGWVRVPANYAALSESKE

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=383, Percent_Identity=33.6814621409922, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 42071; Mature: 42071

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNETVPQIRIPATYMRGGTSKGVFFRLNDLPLEAQLPGAARDALLLRVIGSPDPYGKQID
CCCCCCEEECCHHHHCCCCCCCEEEEEECCCEEEECCCCHHCEEEEEEECCCCCCCCCCC
GMGGATSSTSKTVIVSASSRTDHDVDYLFGQVAIDKPFVDWSGNCGNLSAAVGPFAIHAG
CCCCCCCCCCCEEEEEECCCCCCCHHHHHEEEEECCCCCCCCCCCCCCCCCCCCHHEECC
LIPAECIPHNGVVAVKVWQVNISKTIVVHVPIVNGAVQECGEFELDGVTFAAAEVQVDFV
CCCHHHCCCCCEEEEEEEEEECCEEEEEEEEECCCHHHHCCCEEECCEEEEEEEEEEEEE
DPADDSGAMFPTGNVMDDLQVPGIGTLNATFINSGIPTIFVDAEALGYQGTELQEAINSD
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEHHHHCCCCCHHHHHHCCC
PQALAKFETLRAYGALKMGLIERIEEAQNRQHTPKIAFVSGAKSYLASSGKRVEADSVDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCCEEECCHHHH
LVRALSMGKLHHAMMGTAAVAIASAAAVPGTLVNLAAGGGERTSVTFGHPSGTLKVGAKA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCEEEECCCC
KQGKQGWQIERAIMSRSARILMEGWVRVPANYAALSESKE
CCCCCCHHHHHHHHHCCHHEEEECCEECCCCHHHHCCCCC
>Mature Secondary Structure
MNETVPQIRIPATYMRGGTSKGVFFRLNDLPLEAQLPGAARDALLLRVIGSPDPYGKQID
CCCCCCEEECCHHHHCCCCCCCEEEEEECCCEEEECCCCHHCEEEEEEECCCCCCCCCCC
GMGGATSSTSKTVIVSASSRTDHDVDYLFGQVAIDKPFVDWSGNCGNLSAAVGPFAIHAG
CCCCCCCCCCCEEEEEECCCCCCCHHHHHEEEEECCCCCCCCCCCCCCCCCCCCHHEECC
LIPAECIPHNGVVAVKVWQVNISKTIVVHVPIVNGAVQECGEFELDGVTFAAAEVQVDFV
CCCHHHCCCCCEEEEEEEEEECCEEEEEEEEECCCHHHHCCCEEECCEEEEEEEEEEEEE
DPADDSGAMFPTGNVMDDLQVPGIGTLNATFINSGIPTIFVDAEALGYQGTELQEAINSD
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEHHHHCCCCCHHHHHHCCC
PQALAKFETLRAYGALKMGLIERIEEAQNRQHTPKIAFVSGAKSYLASSGKRVEADSVDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCCEEECCHHHH
LVRALSMGKLHHAMMGTAAVAIASAAAVPGTLVNLAAGGGERTSVTFGHPSGTLKVGAKA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCEEEECCCC
KQGKQGWQIERAIMSRSARILMEGWVRVPANYAALSESKE
CCCCCCHHHHHHHHHCCHHEEEECCEECCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA